pET-42a(+)

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pET42a载体基本信息

出品公司: EMD Biosciences (Novagen)
别名: pET42a, pET 42a, pET-42a
质粒类型: 大肠杆菌蛋白表达
表达水平:
克隆方法: 多克隆位点,限制性内切酶
载体大小: 5930 bp
5' 测序引物及序列: T7: 5'-TAATACGACTCACTATAGGG-3'; pGEX-5': 5'-GGGCTGGCAAGCCACGTTTGGTG-3'
3' 测序引物及序列:   T7t: 5'-GCTAGTTATTGCTCAGCGG-3'
载体标签: N-GST, N-His, N-S, C-His, N-Thrombin, N-Factor Xa
载体抗性: 卡那
备注:
pET42a,b,c的差异主要是在多克隆位点处。
产品目录号: 70561-3
稳定性: 瞬时表达
组成型: 组成型
病毒/非病毒: 非病毒

pET42a载体质粒图谱和多克隆位点信息

pet42a载体图谱
pet42a多克隆位点

pET42a载体简介

pET42系列载体是用来进行克隆和高水平表达含有220个氨基酸的GST融合纯化标签的蛋白表达载体。
pET42a载体的单一的多克隆位点见上面的环状质粒图谱。注意:载体序列是以pBR322质粒的编码规矩进行编码的,所以T7蛋白表达区在质粒图谱上面是反向的。T7 RNA聚合酶启动的克隆和表达区域在质粒图谱中也被标注了出来。质粒的F1复制子是被定向的,所以在T7噬菌体聚合酶的作用下,包含有蛋白编码序列的病毒 粒子能够产生,并启动蛋白表达,同时蛋白表达将被T7终止子序列(Cat. No. 69337-3)的作用下终止蛋白翻译。
把表达的基因插入到载体的PshAI限制性内切酶位点时,可以通过Factor Xa蛋白酶将融合表达的GST标签完全切除。
pET Vectors 选择指南

pET42a载体序列

LOCUS       pET-42a(+)    5930 bp     DNA Circular    SYN
DEFINITION  pET-42a(+)
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTOAUTHORAD1|Novagen Inc.|
FEATURES             Location/Qualifiers
     source          1..5930
                     /organism="pET-42 a(+)"
                     /mol_type="other DNA"
     terminator      complement(1..120)
                     /label="T7_terminator"
     misc_feature    69..87
                     /label="T7_Terminal_primer"
     CDS             complement(139..1092)
                     /label="ORF frame 3"
     misc_feature    complement(307..351)
                     /label="S15"
     misc_feature    complement(394..411)
                     /label="6xHIS"
     gene            complement(442..1092)
                     /label="GST (variant)"
                     /gene="GST (variant)"
     CDS             1093..1821
                     /label="ORF frame 1"
     misc_feature    complement(1101..1117)
                     /label="T7_transl_en_RBS"
     misc_feature    complement(1135..1162)
                     /label="lacO"
     promoter        complement(1162..1180)
                     /label="T7_promoter"
     gene            1216..1479
                     /label="tet (300 - 563)"
                     /gene="tet (300 - 563)"
     misc_feature    complement(1268..1287)
                     /label="pBRrevBam_primer"
     misc_feature    1562..2653
                     /label="lacI"
     CDS             1694..2653
                     /label="ORF frame 2"
     misc_feature    3225..3416
                     /label="ROP"
     misc_feature    complement(3410..3432)
                     /label="pGEX_3_primer"
     rep_origin      complement(3831..4450)
                     /label="pBR322_origin"
     gene            4556..5371
                     /label="KanR2"
                     /gene="KanR2"
     CDS             4556..5371
                     /label="ORF frame 2"
ORIGIN
    1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
   61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
  121 tgtttagcag cctaggtatt aatcaattag tggtggtggt ggtggtggtg gtgctcgagt
  181 gcggccgcaa gcttgtcgac ggagctcgcc tgcaggcgcg ccaaggcctg tacagaattc
  241 ggatccccga tatcccatgg acccgcgtcc ctcaataccg gagccaccac cggtacccag
  301 atctgggctg tccatgtgct ggcgttcgaa tttagcagca gcggtttctt tcataccaat
  361 tgcagtacta ccgcgtggca ccagacccgc ggagtgatgg tgatggtgat gaccagaacc
  421 actagttgaa ccatccgatt ttggaggatg gtcgccacca ccaaacgtgg cttgccagcc
  481 ctgcaaaggc catgctatat acttgctgga tttcaagtac ttatcaattt gtgggatagc
  541 ttcaatacgt tttttaaaac aaactaattt tgggaacgca tccaggcaca ttgggtccat
  601 gtataaaaca acatcaagag cgtcatacaa catgaagtca ggatgggtta catgatcacc
  661 atttaaatat gttttatgac ataaacgatc ttcgaacatt ttcagcattt caggtagctt
  721 gctaagaaaa tcaactttga gagtttcaaa gtctttacta tatgcaattc tcgaaacacc
  781 gtatctaata tccaaaaccg ctccttcaag cattgaaatc tctgcacgct cttttggaca
  841 accacccaac atgttgtgct tgtcagctat ataacgtatg atggccatag actgtgttaa
  901 tttaacatca ccatcaatat aataaggaag attgggaaac tccaaaccca attcaaactt
  961 tttgtttcgc catttatcac cttcatcgcg ctcatacaaa tgctcttcat atttttcttc
 1021 aagatattcc aaaagaagtc gagtgggttg cacaaggccc ttaattttcc aataacctag
 1081 tataggggac atatgtatat ctccttctta aagttaaaca aaattatttc tagaggggaa
 1141 ttgttatccg ctcacaattc ccctatagtg agtcgtatta atttcgcggg atcgagatcg
 1201 atctcgatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
 1261 gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
 1321 atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc
 1381 gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta
 1441 ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgagatcc cggacaccat
 1501 cgaatggcgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga gtcaattcag
 1561 ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta
 1621 tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa
 1681 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc
 1741 gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc
 1801 gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc
 1861 gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca
 1921 acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga
 1981 agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa
 2041 cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt
 2101 gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg
 2161 tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga
 2221 aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat
 2281 cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat
 2341 taccgagtcc gggctgcgcg ttggtgcgga catctcggta gtgggatacg acgataccga
 2401 agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg
 2461 gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca
 2521 gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc
 2581 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
 2641 aagcgggcag tgagcgcaac gcaattaatg taagttagct cactcattag gcaccgggat
 2701 ctcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca
 2761 tgactagcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg ggttgcctta
 2821 ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc tgctgcaaaa
 2881 cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg
 2941 aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca ggatgctgct
 3001 ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga ccctgagtga
 3061 tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa cgttccagta
 3121 accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt ttcatcggta
 3181 tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc aaacaggaaa
 3241 aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt ctggagaaac
 3301 tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac gaccacgctg
 3361 atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
 3421 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
 3481 gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta
 3541 gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt
 3601 gcaccatata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
 3661 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
 3721 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
 3781 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
 3841 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
 3901 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
 3961 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
 4021 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
 4081 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
 4141 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
 4201 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
 4261 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
 4321 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
 4381 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
 4441 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
 4501 ttggtcatga acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag
 4561 ccatattcaa cgggaaacgt cttgctctag gccgcgatta aattccaaca tggatgctga
 4621 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg
 4681 attgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc
 4741 caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc
 4801 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc
 4861 cgggaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga
 4921 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa
 4981 cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga
 5041 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat
 5101 gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga
 5161 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat
 5221 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc
 5281 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca
 5341 gtttcatttg atgctcgatg agtttttcta agaattaatt catgagcgga tacatatttg
 5401 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
 5461 ctgaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
 5521 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
 5581 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
 5641 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
 5701 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
 5761 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
 5821 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
 5881 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca
//

pET42a载体质粒图谱pdf版和相关资料下载

pET42a载体应用举例

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