pET-26b(+)

价格:800元

联系方式:I47-825O-882O

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pet26b载体基本信息

出品公司: EMD Biosciences(Novagen)
别名: pET26b, pet-26b(+), pET 26b(+)
质粒类型: 大肠杆菌蛋白表达
表达水平:
克隆方法: 多克隆位点,限制性内切酶
载体大小: 5360bp
5' 测序引物: T7
5' 测序引物序列:   5'-TAATACGACTCACTATAGGG-3'
载体标签: C-His
载体抗性: 卡那(Kanamycin)
备注:
含有pelB信号肽,蛋白表达时,能够将目的基因定位于细胞周质。
产品目录号: 69862-3
稳定性: 瞬时表达 Transient
组成型: 组成型 Constitutive
病毒/非病毒: 非病毒

pet26b载体质粒图谱和多克隆位点信息

pET-26b载体图谱



pET-26b多克隆位点

pET-26b载体特征

Feature Name Start End
T7_terminator 129 1
T7_Terminal_primer 69 87
T7_transl_en_RBS 314 298
lacO 359 332
T7_promoter 377 359
tet (300 - 563) 409 672
pBRrevBam_primer 480 461
lacI 755 1846
tet (576 - 851) 1905 2180
ROP 2655 2846
pGEX_3_primer 2862 2840
pBR322_origin 3880 3261
KanR2 3986 4801

ORF Start End
ORF frame 1 514 1014
ORF frame 2 887 1846
ORF frame 2 3986 4801

Enzyme Name Cut
XhoI 158
NotI 166
EagI 166
HindIII 173
SalI 179
SacI 190
EcoRI 192
BamHI 198
NcoI 220
MscI 225
NdeI 288
XbaI 326
BglII 392
BclI 1128
ApaI 1325
HpaI 1620
FspI 2196
NruI 4074
ClaI 4108
SmaI 4291
XmaI 4289

pet26b载体简介

The pET-26b(+) vector (Cat. No. 69862-3) carries an N-terminal pelB signal sequence for potential periplasmic localization, plus optional C-terminal His•Tag® sequence. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, single-stranded sequencing should be performed using the T7 terminator primer (Cat. No. 69337-3).
pET Vectors 选择指南

pet26b载体序列

LOCUS       pET-26b(+)    5360 bp     DNA    circular    SYN
DEFINITION  pET-26b(+)
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTOAUTHORAD1|Novagen Inc.|
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="pET-26b(+)"
                     /mol_type="other DNA"
     terminator      complement(1..129)
                     /label="T7_terminator"
     misc_feature    69..87
                     /label="T7_Terminal_primer"
     misc_feature    complement(298..314)
                     /label="T7_transl_en_RBS"
     misc_feature    complement(332..359)
                     /label="lacO"
     promoter        complement(359..377)
                     /label="T7_promoter"
     gene            409..672
                     /label="tet (300 - 563)"
                     /gene="tet (300 - 563)"
     misc_feature    complement(461..480)
                     /label="pBRrevBam_primer"
     CDS             514..1014
                     /label="ORF frame 1"
     misc_feature    755..1846
                     /label="lacI"
     CDS             887..1846
                     /label="ORF frame 2"
     gene            1905..2180
                     /label="tet (576 - 851)"
                     /gene="tet (576 - 851)"
     misc_feature    2655..2846
                     /label="ROP"
     misc_feature    complement(2840..2862)
                     /label="pGEX_3_primer"
     rep_origin      complement(3261..3880)
                     /label="pBR322_origin"
     gene            3986..4801
                     /label="KanR2"
                     /gene="KanR2"
     CDS             3986..4801
                     /label="ORF frame 2"
ORIGIN
    1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
   61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
  121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
  181 cgacggagct cgaattcgga tccgaattaa ttccgatatc catggccatc gccggctggg
  241 cagcgaggag cagcagacca gcagcagcgg tcggcagcag gtatttcata tgtatatctc
  301 cttcttaaag ttaaacaaaa ttatttctag aggggaattg ttatccgctc acaattcccc
  361 tatagtgagt cgtattaatt tcgcgggatc gagatctcga tcctctacgc cggacgcatc
  421 gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc
  481 gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg
  541 gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt
  601 gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg
  661 cataagggag agcgtcgaga tcccggacac catcgaatgg cgcaaaacct ttcgcggtat
  721 ggcatgatag cgcccggaag agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt
  781 atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca
  841 ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa
  901 ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt
  961 tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg
 1021 cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc
 1081 ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta
 1141 tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt
 1201 atttcttgat gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg
 1261 tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc
 1321 gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac
 1381 tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt
 1441 tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa
 1501 cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc
 1561 ggatatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtt
 1621 aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca
 1681 actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag
 1741 aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
 1801 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
 1861 atgtaagtta gctcactcat taggcaccgg gatctcgacc gatgcccttg agagccttca
 1921 acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg
 1981 tcttctttat catgcaactc gtaggacagg tgccggcagc gctctgggtc attttcggcg
 2041 aggaccgctt tcgctggagc gcgacgatga tcggcctgtc gcttgcggta ttcggaatct
 2101 tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc
 2161 aggccattat cgccggcatg gcggccccac gggtgcgcat gatcgtgctc ctgtcgttga
 2221 ggacccggct aggctggcgg ggttgcctta ctggttagca gaatgaatca ccgatacgcg
 2281 agcgaacgtg aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg
 2341 tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta
 2401 tgttccggat ctgcatcgca ggatgctgct ggctaccctg tggaacacct acatctgtat
 2461 taacgaagcg ctggcattga ccctgagtga tttttctctg gtcccgccgc atccataccg
 2521 ccagttgttt accctcacaa cgttccagta accgggcatg ttcatcatca gtaacccgta
 2581 tcgtgagcat cctctctcgt ttcatcggta tcattacccc catgaacaga aatccccctt
 2641 acacggaggc atcagtgacc aaacaggaaa aaaccgccct taacatggcc cgctttatca
 2701 gaagccagac attaacgctt ctggagaaac tcaacgagct ggacgcggat gaacaggcag
 2761 acatctgtga atcgcttcac gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt
 2821 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
 2881 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
 2941 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta
 3001 tgcggcatca gagcagattg tactgagagt gcaccatata tgcggtgtga aataccgcac
 3061 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
 3121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
 3181 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
 3241 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
 3301 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
 3361 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
 3421 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
 3481 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
 3541 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
 3601 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
 3661 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
 3721 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
 3781 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
 3841 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
 3901 cagtggaacg aaaactcacg ttaagggatt ttggtcatga acaataaaac tgtctgctta
 3961 cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cttgctctag
 4021 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa
 4081 tgtcgggcaa tcaggtgcga caatctatcg attgtatggg aagcccgatg cgccagagtt
 4141 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact
 4201 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga
 4261 tgatgcatgg ttactcacca ctgcgatccc cgggaaaaca gcattccagg tattagaaga
 4321 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca
 4381 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc
 4441 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg
 4501 ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc
 4561 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat
 4621 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct
 4681 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg
 4741 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta
 4801 agaattaatt catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
 4861 ttccgcgcac atttccccga aaagtgccac ctgaaattgt aaacgttaat attttgttaa
 4921 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
 4981 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
 5041 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
 5101 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
 5161 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
 5221 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
 5281 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
 5341 agggcgcgtc ccattcgcca
//

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