| 质粒名称: | pGTMV |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 118755 |
| 存储实验室: | Jose-Antonio Daros |
|
载体骨架基本信息 |
|
| 载体名称: | pCLEAN-G181 |
| 载体出品公司: | -- |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | 9669 bp |
| 载体修饰: | -- |
| 载体类型: | 植物表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Tobacco mosaic virus,TMV |
| 物种来源: | Tobacco mosaic virus |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | Cauliflower mosaic virus 35S |
| 标签/融合蛋白: | -- |
| 克隆方法: | Gibson克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pGTMV was a gift from Jose-Antonio Daros (Addgene plasmid # 118755 ; http://n2t.net/addgene:118755 ; RRID:Addgene_118755) |
| 参考文献部分: | Efficient production of antifungal proteins in plants using a new transient expression vector derived from tobacco mosaic virus. Shi X, Cordero T, Garrigues S, Marcos JF, Daròs JA, Coca M.. Plant Biotech Journal (2018) 10.1111/pbi.13038 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pGTMV 9669 bp ds-DNA circular SYN 30-NOV-2018
DEFINITION Binary plasmid with a tobacco mosaic virus infectious clone.
ACCESSION .
VERSION .
KEYWORDS pGTMV
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9669)
TITLE Tobacco mosaic virus-derived system to express recombinant proteins
in plants with Gibson assembly
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 9669)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Nov 30, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9669
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 36..56
/label=35S promoter
/note="CaMV 35S promoter, forward primer"
promoter 45..90
/label=minimal CaMV 35S promoter
/note="minimal 35S promoter from cauliflower mosaic virus"
misc_feature 101..156
/label=TMV Omega
/note="translational enhancer from the tobacco mosaic virus
5'-leader sequence (Gallie et al., 1988)"
misc_feature 6774..6798
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
rep_origin complement(6917..7505)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6997..7016)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7676..8491)
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
primer_bind 8399..8418
/label=Kan-R
/note="Kanamycin resistance gene, reverse primer"
primer_bind 8483..8502
/label=pENTR-R
/note="pENTR vectors, reverse primer"
rep_origin 8782..9217
/label=pSa ori
/note="origin of replication from bacterial plasmid pSa"
misc_feature 9368..9392
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
misc_feature 9419..9443
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
ORIGIN
1 atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct
61 atataaggaa gttcatttca tttggagagg tatttttaca acaattacca acaacaacaa
121 acaacaaaca acattacaat tactatttac aattacaatg gcatacacac agacagctac
181 cacatcagct ttgctggaca ctgtccgagg aaacaactcc ttggtcaatg atctagcaaa
241 gcgtcgtctt tacgacacag cggttgaaga gtttaacgct cgtgaccgca ggcccaaggt
301 gaacttttca aaagtaataa gcgaggagca gacgcttatt gctacccggg cgtatccaga
361 attccaaatt acattttata acacgcaaaa tgccgtgcat tcgcttgcag gtggattgcg
421 atctttagaa ctggaatatc tgatgatgca aattccctac ggatcattga cttatgacat
481 aggcgggaat tttgcatcgc atctgttcaa gggacgagca tatgtacact gctgcatgcc
541 caacctggac gttcgagaca tcatgcggca cgaaggccag aaagacagta ttgaactata
601 cctttctagg ctagagagag gggggaaaac agtccccaac ttccaaaagg aagcatttga
661 cagatacgca gaaattcctg aagacgctgt ctgtcacaat actttccaga catgcgaaca
721 tcagccgatg cagcaatcag gcagagtgta tgccattgcg ctacacagca tatatgacat
781 accagccgat gagttcgggg cggcactctt gaggaaaaat gtccatacgt gctatgccgc
841 tttccacttc tccgagaacc tgcttcttga agattcatgc gtcaatttgg acgaaatcaa
901 cgcgtgtttt tcgcgcgatg gagacaagtt gaccttttct tttgcatcag agagtactct
961 taattactgt catagttatt ctaatattct taagtatgtg tgcaaaactt acttcccggc
1021 ctctaataga gaggtttaca tgaaggagtt tttagtcacc agagttaata cctggttttg
1081 taagttttct agaatagata cttttctttt gtacaaaggt gtggcccata aaagtgtaga
1141 tagtgagcag ttttatactg caatggaaga cgcatggcat tacaaaaaga ctcttgcaat
1201 gtgcaacagc gagagaatcc tccttgagga ttcatcatca gtcaattact ggtttcccaa
1261 aatgagggat atggtcatcg taccattatt cgacatttct ttggagacta gtaagaggac
1321 gcgcaaggaa gtcttagtgt ccaaggattt cgtgtttaca gtgcttaacc acattcgaac
1381 ataccaggcg aaagctctta catacgcaaa tgttttgtcc ttcgtcgaat cgattcgatc
1441 gagggtaatc attaacggtg tgacagcgag gtccgaatgg gatgtggaca aatctttgtt
1501 acaatccttg tccatgacgt tttacctgca tactaagctt gccgttctaa aggatgactt
1561 actgattagc aagtttagtc tcggttcgaa aacggtgtgc cagcatgtgt gggatgagat
1621 ttcgctggcg tttgggaacg catttccctc cgtgaaagag aggctcttga acaggaaact
1681 tatcagagtg gcaggcgacg cattagagat cagggtgcct gatctatatg tgaccttcca
1741 cgacagatta gtgactgagt acaaggcctc tgtggacatg cctgcgcttg acattaggaa
1801 gaagatggaa gaaacggaag tgatgtacaa tgcactttca gaattatcgg tgttaaggga
1861 gtctgacaaa ttcgatgttg atgttttttc ccagatgtgc caatctttgg aagttgaccc
1921 aatgacggca gcgaaggtta tagtcgcggt catgagcaat gagagcggtc tgactctcac
1981 atttgaacga cctactgagg cgaatgttgc gctagcttta caggatcaag agaaggcttc
2041 agaaggtgca ttggtagtta cctcaagaga agttgaagaa ccgtccatga agggttcgat
2101 ggccagagga gagttacaat tagctggtct tgctggagat catccggaat cgtcctattc
2161 taagaacgag gagatagagt ctttagagca gtttcatatg gcgacggcag attcgttaat
2221 tcgtaagcag atgagctcga ttgtgtacac gggtccgatt aaagttcagc aaatgaaaaa
2281 ctttatcgat agcctggtag catcactatc tgctgcggtg tcgaatctcg tcaagatcct
2341 caaagataca gctgctattg accttgaaac ccgtcaaaag tttggagtct tggatgttgc
2401 atctaggaag tggttaatca aaccaacggc caagagtcat gcatggggtg ttgttgaaac
2461 ccacgcgagg aagtatcatg tggcgctttt ggaatatgat gagcagggtg tggtgacatg
2521 cgatgattgg agaagagtag ctgttagctc tgagtctgtt gtttattccg acatggcgaa
2581 actcagaact ctgcgcagac tgcttcgaaa cggagaaccg catgtcagta gcgcaaaggt
2641 tgttcttgtg gacggagttc cgggctgtgg aaaaaccaaa gaaattcttt ccagggttaa
2701 ttttgatgaa gatctaattt tagtacctgg gaagcaagcc gcggaaatga tcagaagacg
2761 tgcgaattcc tcagggatta ttgtggccac gaaggacaac gttaaaaccg ttgattcttt
2821 catgatgaat tttgggaaaa gcacacgctg tcagttcaag aggttattca ttgatgaagg
2881 gttgatgttg catactggtt gtgttaattt tcttgtggcg atgtcattgt gcgaaattgc
2941 atatgtttac ggagacacac agcagattcc atacatcaat agagtttcag gattcccgta
3001 ccccgcccat tttgccaaat tggaagttga cgaggtggag acacgcagaa ctactctccg
3061 ttgtccagcc gatgtcacac attatctgaa caggagatat gagggctttg tcatgagcac
3121 ttcttcggtt aaaaagtctg tttcgcagga gatggtcggc ggagccgccg tgatcaatcc
3181 gatctcaaaa cccttgcatg gcaagatcct gacttttacc caatcggata aagaagctct
3241 gctttcaaga gggtattcag atgttcacac tgtgcatgaa gtgcaaggcg agacatactc
3301 tgatgtttca ctagttaggt taacccctac accggtctcc atcattgcag gagacagccc
3361 acatgttttg gtcgcattgt caaggcacac ctgttcgctc aagtactaca ctgttgttat
3421 ggatccttta gttagtatca ttagagatct agagaaactt agctcgtact tgttagatat
3481 gtataaggtc gatgcaggaa cacaatagca attacagatt gactcggtgt tcaaaggttc
3541 caatcttttt gttgcagcgc caaagactgg tgatatttct gatatgcagt tttactatga
3601 taagtgtctc ccaggcaaca gcaccatgat gaataatttt gatgctgtta ccatgaggtt
3661 gactgacatt tcattgaatg tcaaagattg catattggat atgtctaagt ctgttgctgc
3721 gcctaaggat caaatcaaac cactaatacc tatggtacga acggcggcag aaatgccacg
3781 ccagactgga ctattggaaa atttagtggc gatgattaaa agaaacttta acgcacccga
3841 gttgtctggc atcattgata ttgaaaatac tgcatctttg gttgtagata agttttttga
3901 tagttatttg cttaaagaaa aaagaaaacc aaataaaaat gtttctttgt tcagtagaga
3961 gtctctcaat agatggttag aaaagcagga acaggtaaca ataggccagc tcgcagattt
4021 tgattttgtg gatttgccag cagttgatca gtacagacac atgattaaag cacaacccaa
4081 acaaaagttg gacacttcaa tccaaacgga gtacccggct ttgcagacga ttgtgtacca
4141 ttcaaaaaag atcaatgcaa tattcggccc gttgtttagt gagcttacta ggcaattact
4201 ggacagtgtt gattcgagca gatttttgtt tttcacaaga aagacaccag cgcagattga
4261 ggatttcttc ggagatctcg acagtcatgt gccgatggat gtcttggagc tggatatatc
4321 aaaatacgac aaatctcaga atgaattcca ctgtgcagta gaatacgaga tctggcgaag
4381 attgggtttt gaagacttct tgggagaagt ttggaaacaa gggcatagaa agaccaccct
4441 caaggattat accgcaggta taaaaacttg catctggtat caaagaaaga gcggggacgt
4501 cacgacgttc attggaaaca ctgtgatcat tgctgcatgt ttggcctcga tgcttccgat
4561 ggagaaaata atcaaaggag ccttttgcgg tgacgatagt ctgctgtact ttccaaaggg
4621 ttgtgagttt ccggatgtgc aacactccgc aaatcttatg tggaattttg aagcaaaact
4681 gtttaaaaaa cagtatggat acttttgcgg aagatatgta atacatcacg acagaggatg
4741 cattgtgtat tacgatcccc taaagttgat ctcgaaactt ggtgctaaac acatcaagga
4801 ttgggaacac ttggaggagt tcagaaggtc tctttgtgat gttgctgttt cgttgaacaa
4861 ttgtgcgtat tacacacagt tggacgacgc tgtatgggag gttcataaga ccgcccctcc
4921 aggttcgttt gtttataaaa gtctggtgaa gtatttgtct gataaagttc tttttagaag
4981 tttgtttata gatggctcta gttgttaaag gaaaagtgaa tatcaatgag tttatcgacc
5041 tgtcaaaaat ggagaagatc ttaccgtcga tgtttacccc tgtaaagagt gttatgtgtt
5101 ccaaagttga taaaataatg gttcatgaga atgagtcatt gtcagaggtg aaccttctta
5161 aaggagttaa gcttattgat agtggatacg tctgtttagc cggtttggtc gtcacgggcg
5221 agtggaactt gcctgacaat tgcagaggag gtgtgagcgt gtgtctggtg gacaaaagga
5281 tggaaagagc cgacgaggcc actctcggat cttactacac agcagctgca aagaaaagat
5341 ttcagttcaa ggtcgttccc aattatgcta taaccaccca ggacgcgatg aaaaacgtct
5401 ggcaagtttt agttaatatt agaaatgtga agatgtcagc gggtttctgt ccgctttctc
5461 tggagtttgt gtcggtgtgt attgtttata gaaataatat aaaattaggt ttgagagaga
5521 agattacaaa cgtgagagac ggagggccca tggaacttac agaagaagtc gttgatgagt
5581 tcatggaaga tgtccctatg tcgatcaggc ttgcaaagtt tcgatctcga accggaaaaa
5641 agagtgatgt ccgcaaaggg aaaaatagta gtagtgatcg gtcagtgccg aacaagaact
5701 atagaaatgt taaggatttt ggaggaatga gttttaaaaa gaataattta atcgatgatg
5761 attcggaggc tactgtcgcc gaatcggatt cgttttaaat atgtcttaca gtatcactac
5821 tccatctcag ttcgtgttct tgtcatcagc gtgggccgac ccaatagagt taattaattt
5881 atgtactaat gccttaggaa atcagtttca aacacaacaa gctcgaactg tcgttcaaag
5941 acaattcagt gaggtgtgga aaccttcacc acaagtaact gttaggttcc ctgacagtga
6001 ctttaaggtg tacaggtaca atgcggtatt agacccgcta gtcacagcac tgttaggtgc
6061 attcgacact agaaatagaa taatagaagt tgaaaatcag gcgaacccca cgactgccga
6121 gacgttagat gctactcgta gagtagacga cgcaacggtg gccataagga gcgcgataaa
6181 taatttaata gtagaattga tcagaggaac cggatcttat aatcggagct ctttcgagag
6241 ctcttctggt ttggtttgga cctctggtcc tgcaacttga ggtagtcaag atgcataata
6301 aataacggat tgtgtccgta atcacacgtg gtgcgtacga taacgcatag tgtttttccc
6361 tccacttaaa tcgaagggtt gtgtcttgga tcgcgcgggt caaatgtata tggttcatat
6421 acatccgcag gcacgtaata aagcgagggg ttcgaatccc cccgttaccc ccggtagggg
6481 cccagggtcg gcatggcatc tccacctcct cgcggtccga cctgggctac ttcggtaggc
6541 taagggagaa gcgctgaaat caccagtctc tctctacaaa tctatctctc tctattttct
6601 ccataaataa tgtgtgagta gtttcccgat aagggaaatt agggttctta tagggtttcg
6661 ctcatgtgtt gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta
6721 tcaataaaat ttctaattcc taaaaccaaa atccaggggc cctcgacgtt ccttgacagg
6781 atatattggc gggtaaacta agtcgctgta tgtgtttgtt tgagatcctc tagggcatgc
6841 aagctgatct ggatctcatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
6901 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
6961 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
7021 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
7081 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
7141 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
7201 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
7261 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
7321 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
7381 tgctgaagcc agttaccttc ggaagaagag ttggtagctc ttgatccggc aaacaaacca
7441 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
7501 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
7561 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
7621 aaaaatgaag ttttaaatca atctaaagta tatatgtgta acattggtct agtgattaga
7681 aaaactcatc gagcatcaaa tgaaactgta atttattcat atcaggatta tcaataccat
7741 atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga
7801 tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta
7861 atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat
7921 ccggtgagaa tggcaaaagt ttatgcattt ctttccagac ttgttcaaca ggccagccat
7981 tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct
8041 gagcaagacg aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca
8101 accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt
8161 ctaatacctg gaatgctgtt ttccctggga tcgcagtggt gagtaaccat gcatcatcag
8221 gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc
8281 tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact
8341 ctggcgcatc gggcttccca tacaatcggt agattgtcgc acctgattgc ccgacattat
8401 cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat cgcggccttg
8461 agcaagacgt ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag
8521 cagacagttt tattgttcat gatgatatat ttttatcttg tgcaatgtaa catcagagat
8581 tttgagacac aacgtggctt tgttgaataa atcgaacttt tgctgagttg aaggatcaga
8641 tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa gcaaaagttc aaaatcacca
8701 actggtccac ctacaacaaa gctctcatca accgtggctc cctcactttc tggctggatg
8761 atggggcgat tcaggcgatc cccatccaac agcccgccgt cgagcgggct tttttatccc
8821 cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc cgcaaagcct
8881 tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg ctaatcaggg
8941 tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg aaatcgctaa
9001 tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc aaacacccct
9061 cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat tatgaatata
9121 tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac cggctccgcc
9181 cgtggacaac cgcaagcggt tgcccaccgt cgagcgcctt tgcccacaac ccggcggccg
9241 gccgcaacag atcgttttat aaattttttt ttttgaaaaa gaaaaagccc gaaaggcggc
9301 aacctctcgg gcttctggat ttccgatccc cggaattaga tccgtttaaa ctacgtaaga
9361 tcgatcttgg caggatatat tgtggtgtaa acgttcctgc ggcggtcgag atggatcttg
9421 gcaggatata ttgtggtgta aacgttcctg cggccgcgat tccattgccc agctatctgt
9481 cactttattg tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca tcattgcgat
9541 aaaggaaagg ccatcgttga agatgcctct gccgacagtg gtcccaaaga tggaccccca
9601 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
9661 tgatgtgat
//
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