pZsGreen1-DR

价格:2500元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Clontech
载体名称: pZsGreen1-DR
质粒类型: 无启动子载体;荧光报告载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: 无启动子
载体大小: 4251 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: 绿色荧光蛋白ZsGreen1-DR
载体抗性: 卡那霉素
筛选标记: 新霉素(Neomycin)
克隆菌株: DH5α, JM109等
宿主细胞(系): 原核细胞和真核细胞均可
备注: pZsGreen1-DR载体不含启动子,将待研究的目的启动子插入MCS区,用于研究启动子及顺式转录元件在细胞内的转录水平和活性;
既 可用于原核细胞有可用于真核细胞;
ZsGreen1-DR与ZsGreen1相比,半衰期短,适用于需要报告基因逆转速度快的研究领域。
产品目录号: 632428
稳定性: 瞬表达 或 稳表达
组成型/诱导型: --
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pZsGreen1-DR载体图谱



pZsGreen1-DR 多克隆位点

pZsGreen1-DR 载体特征

载体简介

pZsGreen1-DR载体描述

pZsGreen1-DR is a promoterless vector that encodes ZsGreen1-DR, a destabilized variant of
Zoanthus sp. green fluorescent protein (ZsGreen; 1). The ZsGreen1 coding sequence contains
a series of silent base-pair changes, which correspond to human codon-usage preferences, for
optimal expression in mammalian cells (2). In contrast to the original protein, ZsGreen1-DR has a
short half-life, making it well suited for studies that require rapid reporter turnover. This destabilized
variant was constructed by fusing the C-terminus of the protein to amino acid residues 422–461 of
mouse ornithine decarboxylase (MODC), one of the most short-lived proteins in mammalian cells (3).
This region of MODC contains a PEST sequence that targets the protein for degradation, resulting
in rapid protein turnover (3,4). Three point mutations in this sequence further reduce the protein
half-life (3). Sequences upstream of ZsGreen1-DR have been converted to a Kozak consensus
translation initiation site (5) to enhance translation efficiency in eukaryotic cells. A single amino acid
substitution (Asn-65 to Met) has been made to enhance the emission characteristics of ZsGreen1
(excitation maximum = 496 nm; emission maximum = 506 nm).
pZsGreen1-DR can be used to monitor transcription from different promoters and promoter/enhancer
combinations inserted into the multiple cloning site (MCS), located upstream of the ZsGreen1-DR
coding sequence. Downstream SV40 polyadenylation signals direct proper processing of the 3'
end of the ZsGreen1-DR mRNA. The vector backbone contains an SV40 origin for replication in
mammalian cells expressing the SV40 large T antigen, a pUC origin of replication for propagation in
E. coli, and an f1 origin for single-stranded DNA production. A neomycin-resistance cassette (Neor)
allows stably transfected eukaryotic cells to be selected using G418. This cassette consists of the
SV40 early promoter, the neomycin/kanamycin resistance gene of Tn5, and polyadenylation signals
from the Herpes simplex virus thymidine kinase (HSV TK) gene. A bacterial promoter upstream of
the cassette expresses kanamycin resistance in E. coli.

使用:
ZsGreen1-DR can be used as an in vivo reporter of gene expression. Because of its rapid turnover rate, its expression from a promoter 

of interest provides a more accurate assessment of the promoter's activity over time than does the more stable ZsGreen1. 

Promoter/enhancer elements should be inserted in the MCS upstream of the ZsGreen1-DR coding sequence. Without the addition of a 

functional promoter, this vector will not express ZsGreen1-DR. The recombinant pZsGreen1-DR vector can be transfected into mammalian 

cells using any standard transfection method. If required, stable transformants can be selected using G418 (6).

Location of features
 MCS: 12–83
 Destabilized Zoanthus sp. Green Fluorescent Protein (ZsGreen1-DR) gene
Kozak consensus translation initiation site: 83–93
Start codon (ATG): 90–92; Stop codon: 915–917
Asn-65 to Met mutation (A→T, C→G ): 286, 287
Mouse ornithine decarboxylase PEST sequence: 795–917
 SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1070–1075 & 1099–1104
mRNA 3' ends: 1108 & 1120
 f1 single-strand DNA origin: 1167–1622
(Packages noncoding strand of ZsGreen1-DR.)
 Ampicillin resistance (β-lactamase) promoter
–35 region: 1684–1689; –10 region: 1707–1712
Transcription start point: 1719
 SV40 origin of replication: 1963–2098
 SV40 early promoter
Enhancer (72-bp tandem repeats): 1794–1867 & 1868–1939
21-bp repeats: 1943–1963, 1964–1984 & 1986–2006
Early promoter element: 2019–2025
Major transcription start points: 2015, 2053, 2059 & 2064
 Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2147–2149; stop codon: 2939–2941
G→A mutation to remove Pst I site: 2329
C→A (Arg→Ser) mutation to remove BssH II site: 2675
 Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3177–3182 & 3190–3195
 pUC plasmid replication origin: 3526–4169


Excitation and emission maxima of ZsGreen1
 Excitation maximum = 496 nm
 Emission maximum = 506 nm

载体序列

LOCUS       pZsGreen1-DR	4251 bp 	DNA	SYN
DEFINITION  pZsGreen1-DR
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..4251
                     /organism="pZsGreen1-DR"
                     /mol_type="other DNA"
     CDS             complement(0..800)
                     /label="ORF frame 2"
                     /translation="MAPRKLGQGGAGGDGVLGQVPLLVLGVAAVLAGQLVLDEVPVGH
                     LAGHGLGLVHGVELAAQAATVLQQQVHAHVALQDALLGHGDDLLAGGLPVVGHLLHHG
                     AVGGEVHAVELGLVVHAVLLHAHGDVGVADAHGAVLEQEGAVPGVAGGAGVLEVVDDV
                     LGVLGEHAVAVHEGGGQDVLGEGQGAALHHAQVDGLLALEGVADALAGDHELVAVHAA
                     LHAVLHGHLLGQAVLGLGHGGGSRARGTVDCRIRSLSSRSESGSASNN"
     CDS             90..917
                     /label="ORF frame 3"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAI
                     NLCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDG
                     AVCICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGI
                     LKGDVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWH
                     LTEHAIASGSALPKLPRSHGFPPAVAAQDDGTLPMSCAQESGMDRHPAACASARINV*"
     gene            93..782
                     /label="Zs_Green"
                     /gene="Zs_Green"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAI
                     NLCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDG
                     AVCICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGI
                     LKGDVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWH
                     LTEHAIASGSALPKLPRSHGFPPAVAAQDDGTLPMSCAQESGMDRHPAACASARINV*"
     misc_feature    1130..1149
                     /label="EBV_rev_primer"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAI
                     NLCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDG
                     AVCICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGI
                     LKGDVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWH
                     LTEHAIASGSALPKLPRSHGFPPAVAAQDDGTLPMSCAQESGMDRHPAACASARINV*"
     rep_origin      complement(1299..1605)
                     /label="f1_origin"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAI
                     NLCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDG
                     AVCICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGI
                     LKGDVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWH
                     LTEHAIASGSALPKLPRSHGFPPAVAAQDDGTLPMSCAQESGMDRHPAACASARINV*"
     promoter        1684..1712
                     /label="AmpR_promoter"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAI
                     NLCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDG
                     AVCICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGI
                     LKGDVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWH
                     LTEHAIASGSALPKLPRSHGFPPAVAAQDDGTLPMSCAQESGMDRHPAACASARINV*"
     misc_feature    complement(1778..1798)
                     /label="pBABE_3_primer"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAI
                     NLCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDG
                     AVCICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGI
                     LKGDVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWH
                     LTEHAIASGSALPKLPRSHGFPPAVAAQDDGTLPMSCAQESGMDRHPAACASARINV*"
     misc_feature    complement(1784..1999)
                     /label="SV40_enhancer"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAI
                     NLCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDG
                     AVCICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGI
                     LKGDVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWH
                     LTEHAIASGSALPKLPRSHGFPPAVAAQDDGTLPMSCAQESGMDRHPAACASARINV*"
     promoter        1796..2064
                     /label="SV40_promoter"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAI
                     NLCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDG
                     AVCICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGI
                     LKGDVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWH
                     LTEHAIASGSALPKLPRSHGFPPAVAAQDDGTLPMSCAQESGMDRHPAACASARINV*"
     rep_origin      1963..2040
                     /label="SV40_origin"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAI
                     NLCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDG
                     AVCICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGI
                     LKGDVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWH
                     LTEHAIASGSALPKLPRSHGFPPAVAAQDDGTLPMSCAQESGMDRHPAACASARINV*"
     misc_feature    2025..2044
                     /label="SV40pro_F_primer"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAI
                     NLCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDG
                     AVCICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGI
                     LKGDVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWH
                     LTEHAIASGSALPKLPRSHGFPPAVAAQDDGTLPMSCAQESGMDRHPAACASARINV*"
     CDS             2147..2941
                     /label="ORF frame 2"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     gene            2150..2938
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     CDS             complement(2456..2992)
                     /label="ORF frame 3"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     terminator      3116..3385
                     /label="TK_PA_terminator"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     rep_origin      3533..4152
                     /label="pBR322_origin"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
ORIGIN
    1 TAGTTATTAC TAGCGCTACC GGACTCAGAT CTCGAGCTCA AGCTTCGAAT TCTGCAGTCG
   61 ACGGTACCGC GGGCCCGGGA TCCGCCACCA TGGCCCAGTC CAAGCACGGC CTGACCAAGG
  121 AGATGACCAT GAAGTACCGC ATGGAGGGCT GCGTGGACGG CCACAAGTTC GTGATCACCG
  181 GCGAGGGCAT CGGCTACCCC TTCAAGGGCA AGCAGGCCAT CAACCTGTGC GTGGTGGAGG
  241 GCGGCCCCTT GCCCTTCGCC GAGGACATCT TGTCCGCCGC CTTCATGTAC GGCAACCGCG
  301 TGTTCACCGA GTACCCCCAG GACATCGTCG ACTACTTCAA GAACTCCTGC CCCGCCGGCT
  361 ACACCTGGGA CCGCTCCTTC CTGTTCGAGG ACGGCGCCGT GTGCATCTGC AACGCCGACA
  421 TCACCGTGAG CGTGGAGGAG AACTGCATGT ACCACGAGTC CAAGTTCTAC GGCGTGAACT
  481 TCCCCGCCGA CGGCCCCGTG ATGAAGAAGA TGACCGACAA CTGGGAGCCC TCCTGCGAGA
  541 AGATCATCCC CGTGCCCAAG CAGGGCATCT TGAAGGGCGA CGTGAGCATG TACCTGCTGC
  601 TGAAGGACGG TGGCCGCTTG CGCTGCCAGT TCGACACCGT GTACAAGGCC AAGTCCGTGC
  661 CCCGCAAGAT GCCCGACTGG CACTTCATCC AGCACAAGCT GACCCGCGAG GACCGCAGCG
  721 ACGCCAAGAA CCAGAAGTGG CACCTGACCG AGCACGCCAT CGCCTCCGGC TCCGCCTTGC
  781 CCAAGCTTCC GCGGAGCCAT GGCTTCCCGC CGGCGGTGGC GGCGCAGGAT GATGGCACGC
  841 TGCCCATGTC TTGTGCCCAG GAGAGCGGGA TGGACCGTCA CCCTGCAGCC TGTGCTTCTG
  901 CTAGGATCAA TGTGTAGGCG GCCGCGACTC TAGATCATAA TCAGCCATAC CACATTTGTA
  961 GAGGTTTTAC TTGCTTTAAA AAACCTCCCA CACCTCCCCC TGAACCTGAA ACATAAAATG
 1021 AATGCAATTG TTGTTGTTAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT
 1081 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC
 1141 AAACTCATCA ATGTATCTTA AGGCGTAAAT TGTAAGCGTT AATATTTTGT TAAAATTCGC
 1201 GTTAAATTTT TGTTAAATCA GCTCATTTTT TAACCAATAG GCCGAAATCG GCAAAATCCC
 1261 TTATAAATCA AAAGAATAGA CCGAGATAGG GTTGAGTGTT GTTCCAGTTT GGAACAAGAG
 1321 TCCACTATTA AAGAACGTGG ACTCCAACGT CAAAGGGCGA AAAACCGTCT ATCAGGGCGA
 1381 TGGCCCACTA CGTGAACCAT CACCCTAATC AAGTTTTTTG GGGTCGAGGT GCCGTAAAGC
 1441 ACTAAATCGG AACCCTAAAG GGAGCCCCCG ATTTAGAGCT TGACGGGGAA AGCCGGCGAA
 1501 CGTGGCGAGA AAGGAAGGGA AGAAAGCGAA AGGAGCGGGC GCTAGGGCGC TGGCAAGTGT
 1561 AGCGGTCACG CTGCGCGTAA CCACCACACC CGCCGCGCTT AATGCGCCGC TACAGGGCGC
 1621 GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT
 1681 ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG
 1741 AAAAAGGAAG AGTCCTGAGG CGGAAAGAAC CAGCTGTGGA ATGTGTGTCA GTTAGGGTGT
 1801 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA
 1861 GCAACCAGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT
 1921 CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG
 1981 CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC
 2041 GAGGCCGCCT CGGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA
 2101 GGCTTTTGCA AAGATCGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA
 2161 TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC
 2221 ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC
 2281 GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAAG ACGAGGCAGC
 2341 GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC
 2401 TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC
 2461 TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC
 2521 GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG
 2581 TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT
 2641 CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG CCCGACGGCG AGGATCTCGT
 2701 CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG
 2761 ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC
 2821 CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG
 2881 TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG
 2941 AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT
 3001 TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC
 3061 GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCTAGGGGG
 3121 AGGCTAACTG AAACACGGAA GGAGACAATA CCGGAAGGAA CCCGCGCTAT GACGGCAATA
 3181 AAAAGACAGA ATAAAACGCA CGGTGTTGGG TCGTTTGTTC ATAAACGCGG GGTTCGGTCC
 3241 CAGGGCTGGC ACTCTGTCGA TACCCCACCG AGACCCCATT GGGGCCAATA CGCCCGCGTT
 3301 TCTTCCTTTT CCCCACCCCA CCCCCCAAGT TCGGGTGAAG GCCCAGGGCT CGCAGCCAAC
 3361 GTCGGGGCGG CAGGCCCTGC CATAGCCTCA GGTTACTCAT ATATACTTTA GATTGATTTA
 3421 AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC
 3481 AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA
 3541 GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA
 3601 CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA
 3661 ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC
 3721 CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA
 3781 GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA
 3841 CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG
 3901 CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT
 3961 CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC
 4021 ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC
 4081 CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC
 4141 GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC
 4201 TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCATGCA T

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