pET-41a(+)

价格:800元

联系方式:I47-825O-882O

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pET41a载体基本信息

出品公司: EMD Biosciences (Novagen)
别名: pET41a, pET 41a
质粒类型: 大肠杆菌蛋白表达
表达水平:
克隆方法: 多克隆位点,限制性内切酶
载体大小: 5933 bp
5' 测序引物: pGEX-5': 5'-GGGCTGGCAAGCCACGTTTGGTG-3'
3' 测序引物: T7t: 5'-GCTAGTTATTGCTCAGCGG-3'
载体标签: N-GST, N-His, N-Thrombin, N-EK, N-S
载体抗性: 卡那
表达宿主菌: BL21(DE3) Rosetta2(DE3)C43(DE3)
备注:
载体带有GST蛋白纯化标签,pET41 a,b,c 的差异是在于多克隆位点。
产品目录号: 70556-3
稳定性: 瞬时表达
组成型: 组成型
病毒/非病毒: 非病毒

pET41a载体质粒图谱和多克隆位点信息

pet41a载体图谱
pet41a多克隆位点
pet41a载体克隆方法说明

pET41a载体简介

pET41系列载体是设计用来克隆和高水平表达蛋白质的载体,载体融合表达一个含有220个氨基酸的GST标签蛋白纯化片段。pET41a载体的单一的多克隆位点见上面的环状质粒图谱。注意:载体序列是以pBR322质粒的编码规矩进行编码的,所以T7蛋白表达区在质粒图谱上面是反向的。

T7 RNA聚合酶启动的克隆和表达区域在质粒图谱中也被标注了出来。质粒的F1复制子是被定向的,所以在T7噬菌体聚合酶的作用下,包含有蛋白编码序列的病毒 粒子能够产生,并启动蛋白表达,同时蛋白表达将被T7终止子序列(Cat. No. 69337-3)的作用下终止蛋白翻译。

pET-41a载体上面含有一个EK蛋白酶切位点,当将目的基因使用载体上面的PshAI限制性内切酶位点插入进去时,可以通过EK蛋白酶将载体上的融合的所有氨基酸序列包括GST标签序列,全部切除掉。

pET41a载体序列

LOCUS       pET-41 a(+)    5933 bp     DNA  Circular   SYN
DEFINITION  pET-41 a(+)
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTOAUTHORAD1|Novagen Inc.|
FEATURES             Location/Qualifiers
     source          1..5933
                     /organism="pET-41 a(+)"
                     /mol_type="other DNA"
     terminator      complement(1..120)
                     /label="T7_terminator"
     misc_feature    69..87
                     /label="T7_Terminal_primer"
     CDS             complement(139..1095)
                     /label="ORF frame 3"
     misc_feature    complement(265..279)
                     /label="EK"
     misc_feature    complement(310..354)
                     /label="S15"
     misc_feature    complement(397..414)
                     /label="6xHIS"
     gene            complement(445..1095)
                     /label="GST (variant)"
                     /gene="GST (variant)"
     CDS             1096..1824
                     /label="ORF frame 1"
     misc_feature    complement(1104..1120)
                     /label="T7_transl_en_RBS"
     misc_feature    complement(1138..1165)
                     /label="lacO"
     promoter        complement(1165..1183)
                     /label="T7_promoter"
     gene            1219..1482
                     /label="tet (300 - 563)"
                     /gene="tet (300 - 563)"
     misc_feature    complement(1271..1290)
                     /label="pBRrevBam_primer"
     misc_feature    1565..2656
                     /label="lacI"
     CDS             1697..2656
                     /label="ORF frame 2"
     misc_feature    3228..3419
                     /label="ROP"
     misc_feature    complement(3413..3435)
                     /label="pGEX_3_primer"
     rep_origin      complement(3834..4453)
                     /label="pBR322_origin"
     gene            4559..5374
                     /label="KanR2"
                     /gene="KanR2"
     CDS             4559..5374
                     /label="ORF frame 2"
ORIGIN
    1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
   61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
  121 tgtttagcag cctaggtatt aatcaattag tggtggtggt ggtggtggtg gtgctcgagt
  181 gcggccgcaa gcttgtcgac ggagctcgcc tgcaggcgcg ccaaggcctg tacagaattc
  241 ggatccccga tatcccatgg gactcttgtc gtcgtcatca ccggagccac caccggtacc
  301 cagatctggg ctgtccatgt gctggcgttc gaatttagca gcagcggttt ctttcatacc
  361 aattgcagta ctaccgcgtg gcaccagacc cgcggagtga tggtgatggt gatgaccaga
  421 accactagtt gaaccatccg attttggagg atggtcgcca ccaccaaacg tggcttgcca
  481 gccctgcaaa ggccatgcta tatacttgct ggatttcaag tacttatcaa tttgtgggat
  541 agcttcaata cgttttttaa aacaaactaa ttttgggaac gcatccaggc acattgggtc
  601 catgtataaa acaacatcaa gagcgtcata caacatgaag tcaggatggg ttacatgatc
  661 accatttaaa tatgttttat gacataaacg atcttcgaac attttcagca tttcaggtag
  721 cttgctaaga aaatcaactt tgagagtttc aaagtcttta ctatatgcaa ttctcgaaac
  781 accgtatcta atatccaaaa ccgctccttc aagcattgaa atctctgcac gctcttttgg
  841 acaaccaccc aacatgttgt gcttgtcagc tatataacgt atgatggcca tagactgtgt
  901 taatttaaca tcaccatcaa tataataagg aagattggga aactccaaac ccaattcaaa
  961 ctttttgttt cgccatttat caccttcatc gcgctcatac aaatgctctt catatttttc
 1021 ttcaagatat tccaaaagaa gtcgagtggg ttgcacaagg cccttaattt tccaataacc
 1081 tagtataggg gacatatgta tatctccttc ttaaagttaa acaaaattat ttctagaggg
 1141 gaattgttat ccgctcacaa ttcccctata gtgagtcgta ttaatttcgc gggatcgaga
 1201 tcgatctcga tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg
 1261 gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg
 1321 ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg
 1381 ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta
 1441 ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgaga tcccggacac
 1501 catcgaatgg cgcaaaacct ttcgcggtat ggcatgatag cgcccggaag agagtcaatt
 1561 cagggtggtg aatgtgaaac cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc
 1621 ttatcagacc gtttcccgcg tggtgaacca ggccagccac gtttctgcga aaacgcggga
 1681 aaaagtggaa gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg cacaacaact
 1741 ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc agtctggccc tgcacgcgcc
 1801 gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt
 1861 gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc
 1921 gcaacgcgtc agtgggctga tcattaacta tccgctggat gaccaggatg ccattgctgt
 1981 ggaagctgcc tgcactaatg ttccggcgtt atttcttgat gtctctgacc agacacccat
 2041 caacagtatt attttctccc atgaagacgg tacgcgactg ggcgtggagc atctggtcgc
 2101 attgggtcac cagcaaatcg cgctgttagc gggcccatta agttctgtct cggcgcgtct
 2161 gcgtctggct ggctggcata aatatctcac tcgcaatcaa attcagccga tagcggaacg
 2221 ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg
 2281 catcgttccc actgcgatgc tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc
 2341 cattaccgag tccgggctgc gcgttggtgc ggacatctcg gtagtgggat acgacgatac
 2401 cgaagacagc tcatgttata tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct
 2461 ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg tgaagggcaa
 2521 tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac
 2581 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
 2641 ggaaagcggg cagtgagcgc aacgcaatta atgtaagtta gctcactcat taggcaccgg
 2701 gatctcgacc gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg
 2761 gcatgactag catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc
 2821 ttactggtta gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca
 2881 aaacgtctgc gacctgagca acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc
 2941 tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg gatctgcatc gcaggatgct
 3001 gctggctacc ctgtggaaca cctacatctg tattaacgaa gcgctggcat tgaccctgag
 3061 tgatttttct ctggtcccgc cgcatccata ccgccagttg tttaccctca caacgttcca
 3121 gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag catcctctct cgtttcatcg
 3181 gtatcattac ccccatgaac agaaatcccc cttacacgga ggcatcagtg accaaacagg
 3241 aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca gacattaacg cttctggaga
 3301 aactcaacga gctggacgcg gatgaacagg cagacatctg tgaatcgctt cacgaccacg
 3361 ctgatgagct ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac
 3421 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
 3481 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac
 3541 gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag
 3601 agtgcaccat atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
 3661 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
 3721 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
 3781 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
 3841 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
 3901 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
 3961 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
 4021 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
 4081 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
 4141 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
 4201 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
 4261 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
 4321 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
 4381 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
 4441 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
 4501 attttggtca tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat
 4561 gagccatatt caacgggaaa cgtcttgctc taggccgcga ttaaattcca acatggatgc
 4621 tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta
 4681 tcgattgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt
 4741 tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct
 4801 tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat
 4861 ccccgggaaa acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt
 4921 tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt
 4981 taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt
 5041 tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga
 5101 aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact
 5161 tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg
 5221 aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc
 5281 ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt
 5341 gcagtttcat ttgatgctcg atgagttttt ctaagaatta attcatgagc ggatacatat
 5401 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
 5461 cacctgaaat tgtaaacgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
 5521 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
 5581 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
 5641 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
 5701 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
 5761 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
 5821 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
 5881 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg cca

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pET41a载体质粒图谱pdf版和相关资料下载

pET41a载体应用举例

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