| 质粒名称: | M50 Super 8x TOPFlash |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 12456 |
| 存储实验室: | Randall Moon |
|
载体骨架基本信息 |
|
| 载体名称: | pTA-Luc |
| 载体出品公司: | Clontech |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 4871 bp |
| 载体类型: | 荧光素酶表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | TCF/LEF binding sites |
| 插入大小: | 120 bp |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | MluI(未知是否被破坏) |
| 3' 克隆位点: | MluI(未知是否被破坏) |
| 5' 测序引物 | RVPrimer3 |
| 3' 测序引物 | LucNRev |
|
文献引用方法 |
|
| 材料和方法部分: | M50 Super 8x TOPFlash was a gift from Randall Moon (Addgene plasmid # 12456) |
| 参考文献部分: | Zebrafish prickle, a modulator of noncanonical Wnt/Fz signaling, regulates gastrulation movements. Veeman MT, Slusarski DC, Kaykas A, Louie SH, Moon RT. Curr Biol. 2003 Apr 15. 13(8):680-5. 10.1016/S0960-9822(03)00240-9 PubMed 12699626 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS M50 Super 8x TOPFlash 4985 bp DNA SYN
DEFINITION M50 Super 8x TOPFlash
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..4985
/organism="M50 Super 8x TOPFlash"
/mol_type="other DNA"
gene 254..1906
/label="firefly_Luciferase"
/gene="firefly_Luciferase"
CDS 254..1906
/label="ORF frame 2"
misc_feature complement(320..338)
/label="LucNrev_primer"
misc_feature complement(1958..1977)
/label="EBV_rev_primer"
gene complement(3247..4107)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(3247..4107)
/label="ORF frame 3"
promoter complement(4149..4177)
/label="AmpR_promoter"
rep_origin 4256..4562
/label="f1_origin"
misc_feature 4825..4978
/label="TB"
terminator 4825..4873
/label="synth_PA_terminator"
terminator 4825..4978
/label="up_PA_terminator"
misc_feature 4927..4946
/label="RVprimer3_primer"
ORIGIN
1 ggtaccgagc tcttacgcga gatcaaaggg ggtaagatca aagggggtaa gatcaaaggg
61 gcgcgagatc aaagggggta agatcaaagg gggtaagatc aaagggggta agatcaaagg
121 ggcgcgcccg cgtgctagcc cgggctcgag atctagactc tagagggtat ataatggaag
181 ctcgaattcc agcttggcat tccggtactg ttggtaaaaa gcttggcatt ccggtactgt
241 tggtaaagcc accatggaag acgccaaaaa cataaagaaa ggcccggcgc cattctatcc
301 gctggaagat ggaaccgctg gagagcaact gcataaggct atgaagagat acgccctggt
361 tcctggaaca attgctttta cagatgcaca tatcgaggtg gacatcactt acgctgagta
421 cttcgaaatg tccgttcggt tggcagaagc tatgaaacga tatgggctga atacaaatca
481 cagaatcgtc gtatgcagtg aaaactctct tcaattcttt atgccggtgt tgggcgcgtt
541 atttatcgga gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa
601 cagtatgggc atttcgcagc ctaccgtggt gttcgtttcc aaaaaggggt tgcaaaaaat
661 tttgaacgtg caaaaaaagc tcccaatcat ccaaaaaatt attatcatgg attctaaaac
721 ggattaccag ggatttcagt cgatgtacac gttcgtcaca tctcatctac ctcccggttt
781 taatgaatac gattttgtgc cagagtcctt cgatagggac aagacaattg cactgatcat
841 gaactcctct ggatctactg gtctgcctaa aggtgtcgct ctgcctcata gaactgcctg
901 cgtgagattc tcgcatgcca gagatcctat ttttggcaat caaatcattc cggatactgc
961 gattttaagt gttgttccat tccatcacgg ttttggaatg tttactacac tcggatattt
1021 gatatgtgga tttcgagtcg tcttaatgta tagatttgaa gaagagctgt ttctgaggag
1081 ccttcaggat tacaagattc aaagtgcgct gctggtgcca accctattct ccttcttcgc
1141 caaaagcact ctgattgaca aatacgattt atctaattta cacgaaattg cttctggtgg
1201 cgctcccctc tctaaggaag tcggggaagc ggttgccaag aggttccatc tgccaggtat
1261 caggcaagga tatgggctca ctgagactac atcagctatt ctgattacac ccgaggggga
1321 tgataaaccg ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg ttgtggatct
1381 ggataccggg aaaacgctgg gcgttaatca aagaggcgaa ctgtgtgtga gaggtcctat
1441 gattatgtcc ggttatgtaa acaatccgga agcgaccaac gccttgattg acaaggatgg
1501 atggctacat tctggagaca tagcttactg ggacgaagac gaacacttct tcatcgttga
1561 ccgcctgaag tctctgatta agtacaaagg ctatcaggtg gctcccgctg aattggaatc
1621 catcttgctc caacacccca acatcttcga cgcaggtgtc gcaggtcttc ccgacgatga
1681 cgccggtgaa cttcccgccg ccgttgttgt tttggagcac ggaaagacga tgacggaaaa
1741 agagatcgtg gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt
1801 tgtgtttgtg gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag
1861 agagatcctc ataaaggcca agaagggcgg aaagatcgcc gtgtaattct agagtcgggg
1921 cggccggccg cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac
1981 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
2041 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
2101 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtaa
2161 aatcgataag gatccgtcga ccgatgccct tgagagcctt caacccagtc agctccttcc
2221 ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac
2281 tcgtaggaca ggtgccggca gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
2341 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
2401 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
2461 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
2521 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
2581 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
2641 ctgtccgcct ttTctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
2701 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
2761 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
2821 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
2881 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
2941 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
3001 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
3061 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
3121 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
3181 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
3241 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
3301 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
3361 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
3421 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
3481 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
3541 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
3601 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
3661 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
3721 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
3781 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
3841 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
3901 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
3961 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
4021 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
4081 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
4141 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
4201 aggggttccg cgcacatttc cccgaaaagt gccacctgac gcgccctgta gcggcgcatt
4261 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
4321 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
4381 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
4441 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
4501 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
4561 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
4621 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
4681 aacgCttaca atttGccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
4741 gcgggcctct tcgctattac gccagcccaa gctaccatga taagtaagta atattaaggt
4801 acgggaggta cttggagcgg ccgcaataaa atatctttat tttcattaca tctgtgtgtt
4861 ggttttttgt gtgaatcgat agtactaaca tacgctctcc atcaaaacaa aacgaaacaa
4921 aacaaactag caaaataggc tgtccccagt gcaagtgcag gtgccagaac atttctctat
4981 cgata
//
