pET-14b

价格:800元

联系方式:I47-825O-882O

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pET14b载体基本信息

出品公司: EMD Biosciences(Novagen)
别名: pET14b, pet 14b
质粒类型: 大肠杆菌蛋白表达
表达水平:
克隆方法: 多克隆位点,限制性内切酶
载体大小: 4671bp
5' 测序引物: T7
5' 测序引物序列:   5'-TAATACGACTCACTATAGGG-3'
载体标签: N-His, N-thrombin
载体抗性: Ampicillin
备注:
Hosts: E.coli. Related vectors: pBR322.
产品目录号: 69660-3
稳定性: 瞬时表达 Transient
组成型: 组成型 Constitutive
病毒/非病毒: 非病毒

pET14b载体质粒图谱和多克隆位点信息

pET-14b载体图谱



pET-14b多克隆位点

pET-14b载体特征

Feature Name Start End  
tet (w/ gaps) 86 1584
T7_terminator 507 379
T7_Terminal_primer 447 465
T7_transl_en_RBS 607 591
T7_promoter 662 644
pBRrevBam_primer 765 746
ROP 2223 2414
pGEX_3_primer 2430 2408
Ampicillin 4463 3603
AmpR_promoter 4533 4505
ORF Start End  
ORF frame 1 799 1584
ORF frame 1 1389 931
ORF frame 2 4463 3603
Enzyme Name Cut
ClaI 24
HindIII 29
NheI 229
BamHI 510
XhoI 515
NdeI 522
NcoI 580
XbaI 619
BglII 677
SalI 959
EagI 1247
NruI 1282
MscI 1754
PvuII 2374
PstI 3921
AatII 4598
EcoRI 4669

pET14b载体简介


The pET-14b vector (Cat. No. 69660-3) carries an N-terminal His•Tag® sequence followed by a thrombin site and three cloning sites. Unique sites are shown on the circle map. Note that thesequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below.

pET Vectors 选择指南

pET14b载体序列

LOCUS       pET-14b    4671 bp     DNA  circular    SYN
DEFINITION  pET-14b
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTOAUTHORAD1|Novagen Inc.|
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="pET-14b"
                     /mol_type="other DNA"
     gene            86..1584
                     /label="tet (w/ gaps)"
                     /gene="tet (w/ gaps)"
     terminator      complement(379..507)
                     /label="T7_terminator"
     misc_feature    447..465
                     /label="T7_Terminal_primer"
     misc_feature    complement(591..607)
                     /label="T7_transl_en_RBS"
     promoter        complement(644..662)
                     /label="T7_promoter"
     misc_feature    complement(746..765)
                     /label="pBRrevBam_primer"
     CDS             799..1584
                     /label="ORF frame 1"
                     /translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF
                     LMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFG
                     EDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLA
                     FATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPL
                     IVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
     CDS             complement(931..1389)
                     /label="ORF frame 1"
                     /translation="MVVIYLPGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQ
                     RVGRHAGDNGLLLAETFGGGTSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPER
                     CRHLSYELHDKEDSHKCGDDSHAPRPPEGADWVEGSQGHRSTLSLMRLLH*"
     misc_feature    2223..2414
                     /label="ROP"
                     /translation="MVVIYLPGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQ
                     RVGRHAGDNGLLLAETFGGGTSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPER
                     CRHLSYELHDKEDSHKCGDDSHAPRPPEGADWVEGSQGHRSTLSLMRLLH*"
     misc_feature    complement(2408..2430)
                     /label="pGEX_3_primer"
                     /translation="MVVIYLPGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQ
                     RVGRHAGDNGLLLAETFGGGTSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPER
                     CRHLSYELHDKEDSHKCGDDSHAPRPPEGADWVEGSQGHRSTLSLMRLLH*"
     gene            complement(3603..4463)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MVVIYLPGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQ
                     RVGRHAGDNGLLLAETFGGGTSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPER
                     CRHLSYELHDKEDSHKCGDDSHAPRPPEGADWVEGSQGHRSTLSLMRLLH*"
     CDS             complement(3603..4463)
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(4505..4533)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
   61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
  121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
  181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
  241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
  301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
  361 cacacccgtc ctgtggatat ccggatatag ttcctccttt cagcaaaaaa cccctcaaga
  421 cccgtttaga ggccccaagg ggttatgcta gttattgctc agcggtggca gcagccaact
  481 cagcttcctt tcgggctttg ttagcagccg gatcctcgag catatggctg ccgcgcggca
  541 ccaggccgct gctgtgatga tgatgatgat ggctgctgcc catggtatat ctccttctta
  601 aagttaaaca aaattatttc tagagggaaa ccgttgtggt ctccctatag tgagtcgtat
  661 taatttcgcg ggatcgagat ctcgatcctc tacgccggac gcatcgtggc cggcatcacc
  721 ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg
  781 gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg
  841 gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc
  901 aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt
  961 cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg
 1021 actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg
 1081 gcagcgctct gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc
 1141 ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc
 1201 gccaccaaac gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgctg
 1261 ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt
 1321 ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat
 1381 gacgaccatc agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc
 1441 actggaccgc tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg
 1501 gcatggattg taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca
 1561 tggagccggg ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcacca
 1621 ctccaagaat tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt
 1681 ggcagaacat atccatcgcg tccgccatct ccagcagccg cacgcggcgc atctcgggca
 1741 gcgttgggtc ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag
 1801 gctggcgggg ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa
 1861 gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc
 1921 gtgtttcgta aagtctggaa acgcggaagt cagcgccctg caccattatg ttccggatct
 1981 gcatcgcagg atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct
 2041 ggcattgacc ctgagtgatt tttctctggt cccgccgcat ccataccgcc agttgtttac
 2101 cctcacaacg ttccagtaac cgggcatgtt catcatcagt aacccgtatc gtgagcatcc
 2161 tctctcgttt catcggtatc attaccccca tgaacagaaa tcccccttac acggaggcat
 2221 cagtgaccaa acaggaaaaa accgccctta acatggcccg ctttatcaga agccagacat
 2281 taacgcttct ggagaaactc aacgagctgg acgcggatga acaggcagac atctgtgaat
 2341 cgcttcacga ccacgctgat gagctttacc gcagctgcct cgcgcgtttc ggtgatgacg
 2401 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
 2461 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag
 2521 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga
 2581 gcagattgta ctgagagtgc accatatatg cggtgtgaaa taccgcacag atgcgtaagg
 2641 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
 2701 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
 2761 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
 2821 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
 2881 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
 2941 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
 3001 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
 3061 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
 3121 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
 3181 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
 3241 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
 3301 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
 3361 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
 3421 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
 3481 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
 3541 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
 3601 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
 3661 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
 3721 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
 3781 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
 3841 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
 3901 aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
 3961 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
 4021 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
 4081 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
 4141 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
 4201 tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg
 4261 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
 4321 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
 4381 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
 4441 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
 4501 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
 4561 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
 4621 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga a
//

pET14b载体质粒图谱pdf版和相关资料下载

pET14b载体应用举例

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