pJL-TRBO-G

LOCUS       pJL-TRBO-G               11329 bp ds-DNA     circular SYN 06-AUG-2018
DEFINITION  An improved agroinfection-compatible Tobacco mosaic virus 
            (TMV)-based transient expression vector that lacks the TMV CP gene 
            coding sequence and expresses GFP..
ACCESSION   .
VERSION     .
KEYWORDS    pJL-TRBO-G
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11329)
  AUTHORS   Lindbo JA
  TITLE     TRBO: a high-efficiency tobacco mosaic virus RNA-based 
            overexpression vector.
  JOURNAL   Plant Physiol. 2007 Dec;145(4):1232-40. Epub 2007 Aug 24.
  PUBMED    17720752
REFERENCE   2  (bases 1 to 11329)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11329
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(163..1311)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MAQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(1610..2404)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHLERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      2613..3144
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      2621..3235
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     misc_feature    3328..3352
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     3432..3448
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3433..3449
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4168..4188
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        4177..4222
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_feature    4234..4289
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             9987..10706
                     /codon_start=1
                     /product="Cycle 3 GFP (Crameri et al., 1996)"
                     /label=Cycle 3 GFP
                     /note="mammalian codon-optimized"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(10020..10048)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     10643..10663
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     misc_feature    11313..8
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa agggcgtgaa
       61 aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acaggagatc tcagtaaagc
      121 gctggctgct gaacccccag ccggaactga ccccacaagg ccctagcgtt tgcaatgcac
      181 caggtcatca ttgacccagg cgtgttccac caggccgctg cctcgcaact cttcgcaggc
      241 ttcgccgacc tgctcgcgcc acttcttcac gcgggtggaa tccgatccgc acatgaggcg
      301 gaaggtttcc agcttgagcg ggtacggctc ccggtgcgag ctgaaatagt cgaacatccg
      361 tcgggccgtc ggcgacagct tgcggtactt ctcccatatg aatttcgtgt agtggtcgcc
      421 agcaaacagc acgacgattt cctcgtcgat caggacctgg caacgggacg ttttcttgcc
      481 acggtccagg acgcggaagc ggtgcagcag cgacaccgat tccaggtgcc caacgcggtc
      541 ggacgtgaag cccatcgccg tcgcctgtag gcgcgacagg cattcctcgg ccttcgtgta
      601 ataccggcca ttgatcgacc agcccaggtc ctggcaaagc tcgtagaacg tgaaggtgat
      661 cggctcgccg ataggggtgc gcttcgcgta ctccaacacc tgctgccaca ccagttcgtc
      721 atcgtcggcc cgcagctcga cgccggtgta ggtgatcttc acgtccttgt tgacgtggaa
      781 aatgaccttg ttttgcagcg cctcgcgcgg gattttcttg ttgcgcgtgg tgaacagggc
      841 agagcgggcc gtgtcgtttg gcatcgctcg catcgtgtcc ggccacggcg caatatcgaa
      901 caaggaaagc tgcatttcct tgatctgctg cttcgtgtgt ttcagcaacg cggcctgctt
      961 ggcctcgctg acctgttttg ccaggtcctc gccggcggtt tttcgcttct tggtcgtcat
     1021 agttcctcgc gtgtcgatgg tcatcgactt cgccaaacct gccgcctcct gttcgagacg
     1081 acgcgaacgc tccacggcgg ccgatggcgc gggcagggca gggggagcca gttgcacgct
     1141 gtcgcgctcg atcttggccg tagcttgctg ggccatcgag ccgacggact ggaaggtttc
     1201 gcggggcgca cgcatgacgg tgcggcttgc gatggtttcg gcatcctcgg cggaaaaccc
     1261 cgcgtcgatc agttcttgcc tgtatgcctt ccggtcaaac gtccgattca ttcaccctcc
     1321 ttgcgggatt gccccgactc acgccggggc aatgtgccct tattcctgat ttgacccgcc
     1381 tggtgccttg gtgtccagat aatccacctt atcggcaatg aagtcggtcc cgtagaccgt
     1441 ctggccgtcc ttctcgtact tggtattccg aatcttgccc tgcacgaata ccagcgaccc
     1501 cttgcccaaa tacttgccgt gggcctcggc ctgagagcca aaacacttga tgcggaagaa
     1561 gtcggtgcgc tcctgcttgt cgccggcatc gttgcgccac atctaggtac taaaacaatt
     1621 catccagtaa aatataatat tttattttct cccaatcagg cttgatcccc agtaagtcaa
     1681 aaaatagctc gacatactgt tcttccccga tatcctccct gatcgaccgg acgcagaagg
     1741 caatgtcata ccacttgtcc gccctgccgc ttctcccaag atcaataaag ccacttactt
     1801 tgccatcttt cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct
     1861 cttcgggctt ttccgtcttt aaaaaatcat acagctcgcg cggatcttta aatggggtgt
     1921 cttcttccca gttttcgcaa tccacatcgg ccagatcgtt attcagtaag taatccaatt
     1981 cggctaagcg gctgtctaag ctattcgtat agggacaatc cgatatgtcg atggagtgaa
     2041 agagcctgat gcactccgca tacagctcga taatcttttc agggctttgt tcatcttcat
     2101 actcttccga gcaaaggacg ccatcggcct cactcatgag cagattgctc cagccatcat
     2161 gccgttcaag gtgcaggacc tttggaacag gcagctttcc ttccagccat agcatcatgt
     2221 ccttttcccg ttccacatca taggtggtcc ctttataccg gctgtccgtc atttttaaat
     2281 ataggttttc attttctccc accagcttat ataccttagc aggagacatt ccttccgtat
     2341 cttttacgca gcggtatttt tcgatcagtt ttttcaattc cggtgatatt ctcattttag
     2401 ccatttatta tttccttcct cttttctaca gtatttaaag ataccccaag aagctaatta
     2461 taacaagacg aactccaatt cactgttcct tgcattctaa aactttaaat accagaaaac
     2521 agctttttca aagttgtttt caaagttggc gtataacata gtatcgacgg agccgatttt
     2581 gaaaccacaa ttatgggtga tgctgcaact cgagcgggcc gggagggttc gagaaggggg
     2641 ggcacccccc ttcggcgtgc gcggtcacgc gcacagggcg cagccctggt taaaaacaag
     2701 gtttataaat attggtttaa aagcaggtta aaagacaggt tagcggtggc cgaaaaacgg
     2761 gcggaaaccc ttgcaaatgc tggattttct gcctgtggac agcccctcaa atgtcaatag
     2821 gtgcgcccct catctgtcag cactctgccc ctcaagtgtc aaggatcgcg cccctcatct
     2881 gtcagtagtc gcgcccctca agtgtcaata ccgcagggca cttatcccca ggcttgtcca
     2941 catcatctgt gggaaactcg cgtaaaatca ggcgttttcg ccgatttgcg aggctggcca
     3001 gctccacgtc gccggccgaa atcgagcctg cccctcatct gtcaacgccg cgccgggtga
     3061 gtcggcccct caagtgtcaa cgtccgcccc tcatctgtca gtgagggcca agttttccgc
     3121 gaggtatcca caacgccggc ggccggccgc ggtgtctcgc acacggcttc gacggcgttt
     3181 ctggcgcgtt tgcagggcca tagacggccg ccagcccagc ggcgagggca accagcccgg
     3241 tgagcgtctc tagtggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg
     3301 ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac
     3361 gcttagacaa cttaataaca cattgcggac gtttttaaag tactggggtg gttttggtac
     3421 cgggcccccc ctcgaggtcg acggtatcga taagcttgat atcgaattcc tgcaggtcaa
     3481 catggtggag cacgacacac ttgtctactc caaaaatatc aaagatacag tctcagaaga
     3541 ccaaagggca attgagactt ttcaacaaag ggtaatatcc ggaaacctcc tcggattcca
     3601 ttgcccagct atctgtcact ttattgtgaa gatagtggaa aaggaaggtg gctcctacaa
     3661 atgccatcat tgcgataaag gaaaggccat cgttgaagat gcctctgccg acagtggtcc
     3721 caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc
     3781 ttcaaagcaa gtggattgat gtgataacat ggtggagcac gacacacttg tctactccaa
     3841 aaatatcaaa gatacagtct cagaagacca aagggcaatt gagacttttc aacaaagggt
     3901 aatatccgga aacctcctcg gattccattg cccagctatc tgtcacttta ttgtgaagat
     3961 agtggaaaag gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggccatcgt
     4021 tgaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt
     4081 ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac
     4141 tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg
     4201 aagttcattt catttggaga gggtattttt acaacaatta ccaacaacaa caaacaacag
     4261 acaacattac aattactatt tacaattaca atggcataca cacagacagc taccacatca
     4321 gctttgctgg acactgtccg aggaaacaac tccttggtca atgatctagc aaagcgtcgt
     4381 ctttacgaca cagcggttga agagtttaac gctcgtgacc gcaggcccaa ggtgaacttt
     4441 tcaaaagtaa taagcgagga gcagacgctt attgctaccc gggcgtatcc agaattccaa
     4501 attacatttt ataacacgca aaatgccgtg cattcgcttg caggtggatt gcgatcttta
     4561 gaactggaat atctgatgat gcaaattccc tacggatcat tgacttatga cataggcggg
     4621 aattttgcat cgcatctgtt caagggacga gcatatgtac actgctgcat gcccaacctg
     4681 gacgttcgag acatcatgcg gcacgaaggc cagaaagaca gtattgaact atacctttct
     4741 aggctagaga gaggggggaa aacagtcccc aacttccaaa aggaagcatt tgacagatac
     4801 gcagaaattc ctgaagacgc tgtctgtcac aatactttcc agacatgcga acatcagccg
     4861 atgcagcaat caggcagagt gtatgccatt gcgctacaca gcatatatga cataccagcc
     4921 gatgagttcg gggcggcact cttgaggaaa aatgtccata cgtgctatgc cgctttccac
     4981 ttctccgaga acctgcttct tgaagattca tgcgtcaatt tggacgaaat caacgcgtgt
     5041 ttttcgcgcg atggagacaa gttgaccttt tcttttgcat cagagagtac tcttaattac
     5101 tgtcatagtt attctaatat tcttaagtat gtgtgcaaaa cttacttccc ggcctctaat
     5161 agagaggttt acatgaagga gtttttagtc accagagtta atacctggtt ttgtaagttt
     5221 tctagaatag atacttttct tttgtacaaa ggtgtggccc ataaaagtgt agatagtgag
     5281 cagttttata ctgcaatgga agacgcatgg cattacaaaa agactcttgc aatgtgcaac
     5341 agcgagagaa tcctccttga ggattcatca tcagtcaatt actggtttcc caaaatgagg
     5401 gatatggtca tcgtaccatt attcgacatt tctttggaga ctagtaagag gacgcgcaag
     5461 gaagtcttag tgtccaagga tttcgtgttc acagtgctta accacattcg aacataccag
     5521 gcgaaagctc ttacatacgc aaatgttttg tccttcgtcg aatcgattcg atcgagggta
     5581 atcattaacg gtgtgacagc gaggtccgaa tgggatgtgg acaaatcttt gttacaatcc
     5641 ttgtccatga cgttttacct gcatactaag cttgccgttc taaaggatga cttactgatt
     5701 agcaagttta gtctcggttc gaaaacggtg tgccagcatg tgtgggatga gatttcgctg
     5761 gcgtttggga acgcatttcc ctccgtgaaa gagaggctct tgaacaggaa acttatcaga
     5821 gtggcaggcg acgcattaga gatcagggtg cctgatctat atgtgacctt ccacgacaga
     5881 ttagtgactg agtacaaggc ctctgtggac atgcctgcgc ttgacattag gaagaagatg
     5941 gaagaaacgg aagtgatgta caatgcactt tcagaattat cggtgttaag ggagtctgac
     6001 aaattcgatg ttgatgtttt ttcccagatg tgccaatctt tggaagttga cccaatgacg
     6061 gcagcgaagg ttatagtcgc ggtcatgagc aatgagagcg gtctgactct cacatttgaa
     6121 cgacctactg aggcgaatgt tgcgctagct ttacaggatc aagagaaggc ttcagaaggt
     6181 gcattggtag ttacctcaag agaagttgaa gaaccgtcca tgaagggttc gatggccaga
     6241 ggagagttac aattagctgg tcttgctgga gatcatccgg aatcgtccta ttctaagaac
     6301 gaggagatag agtctttaga gcagtttcat atggcgacgg cagattcgtt aattcgtaag
     6361 cagatgagct cgattgtgta cacgggtccg attaaagttc agcaaatgaa aaactttatc
     6421 gatagcctgg tagcatcact atctgctgcg gtgtcgaatc tcgtcaagat cctcaaagat
     6481 acagctgcta ttgaccttga aacccgtcaa aagtttggag tcttggatgt tgcatctagg
     6541 aagtggttaa tcaaaccaac ggccaagagt catgcatggg gtgttgttga aacccacgcg
     6601 aggaagtatc atgtggcgct tttggaatat gatgagcagg gtgtggtgac atgcgatgat
     6661 tggagaagag tagctgttag ctctgagtct gttgtttatt ccgacatggc gaaactcaga
     6721 actctgcgca gactgcttcg aaacggagaa ccgcatgtca gtagcgcaaa ggttgttctt
     6781 gtggacggag ttccgggctg tggaaaaacc aaagaaattc tttccagggt taattttgat
     6841 gaagatctaa ttttagtacc tgggaagcaa gccgcggaaa tgatcagaag acgtgcgaat
     6901 tcctcaggga ttattgtggc cacgaaggac aacgttaaaa ccgttgattc tttcatgatg
     6961 aattttggga aaagcacacg ctgtcagttc aagaggttat tcattgatga agggttgatg
     7021 ttgcatactg gttgtgttaa ttttcttgtg gcgatgtcat tgtgcgaaat tgcatatgtt
     7081 tacggagaca cacagcagat tccatacatc aatagagttt caggattccc gtaccccgcc
     7141 cattttgcca aattggaagt tgacgaggtg gagacacgca gaactactct ccgttgtcca
     7201 gccgatgtca cacattatct gaacaggaga tatgagggct ttgtcatgag cacttcttcg
     7261 gttaaaaagt ctgtttcgca ggagatggtc ggcggagccg ccgtgatcaa tccgatctca
     7321 aaacccttgc atggcaagat cctgactttt acccaatcgg ataaagaagc tctgctttca
     7381 agagggtatt cagatgttca cactgtgcat gaagtgcaag gcgagacata ctctgatgtt
     7441 tcactagtta ggttaacccc tacaccggtc tccatcattg caggagacag cccacatgtt
     7501 ttggtcgcat tgtcaaggca cacctgttcg ctcaagtact acactgttgt tatggatcct
     7561 ttagttagta tcattagaga tctagagaaa cttagctcgt acttgttaga tatgtataag
     7621 gtcgatgcag gaacacaata gcaattacag attgactcgg tgttcaaagg ttccaatctt
     7681 tttgttgcag cgccaaagac tggtgatatt tctgatatgc agttttacta tgataagtgt
     7741 ctcccaggca acagcaccat gatgaataat tttgatgctg ttaccatgag gttgactgac
     7801 atttcattga atgtcaaaga ttgcatattg gatatgtcta agtctgttgc tgcgcctaag
     7861 gatcaaatca aaccactaat acctatggta cgaacggcgg cagaaatgcc acgccagact
     7921 ggactattgg aaaatttagt ggcgatgatt aaaagaaact ttaacgcacc cgagttgtct
     7981 ggcatcattg atattgaaaa tactgcatct ttggttgtag ataagttttt tgatagttat
     8041 ttgcttaaag aaaaaagaaa accaaataaa aatgtttctt tgttcagtag agagtctctc
     8101 aatagatggt tagaaaagca ggaacaggta acaataggcc agctcgcaga ttttgatttt
     8161 gtggatttgc cagcagttga tcagtacaga cacatgatta aagcacaacc caaacaaaag
     8221 ttggacactt caatccaaac ggagtacccg gctttgcaga cgattgtgta ccattcaaaa
     8281 aagatcaatg caatattcgg cccgttgttt agtgagctta ccaggcaatt actggacagt
     8341 gttgattcga gcagattttt gtttttcaca agaaagacac cagcgcagat tgaggatttc
     8401 ttcggagatc tcgacagtca tgtgccgatg gatgtcttgg agctggatat atcaaaatac
     8461 gacaaatctc agaatgaatt ccactgtgca gtagaatacg agatctggcg aagattgggt
     8521 ttcgaagact tcttgggaga agtttggaaa caagggcata gaaagaccac cctcaaggat
     8581 tataccgcag gtataaaaac ttgcatctgg tatcaaagaa agagcgggga cgtcacgacg
     8641 ttcattggaa acactgtgat cattgctgca tgtttggcct cgatgcttcc gatggagaaa
     8701 ataatcaaag gagccttttg cggtgacgat agtctgctgt actttccaaa gggttgtgag
     8761 tttccggatg tgcaacactc cgcgaatctt atgtggaatt ttgaagcaaa actgtttaaa
     8821 aaacagtatg gatacttttg cggaagatat gtaatacatc acgacagagg atgcattgtg
     8881 tattacgatc ccctaaagtt gatctcgaaa cttggtgcta aacacatcaa ggattgggaa
     8941 cacttggagg agttcagaag gtctctttgt gatgttgctg tttcgttgaa caattgtgcg
     9001 tattacacac agttggacga cgctgtatgg gaggttcata agaccgcccc tccaggttcg
     9061 tttgtttata aaagtctggt gaagtatttg tctgataaag ttctttttag aagtttgttt
     9121 atagatggct ctagttgtta aaggaaaagt gaatatcaat gagtttatcg acctgacaaa
     9181 aatggagaag atcttaccgt cgatgtttac ccctgtaaag agtgttatgt gttccaaagt
     9241 tgataaaata atggttcatg agaatgagtc attgtcaggg gtgaaccttc ttaaaggagt
     9301 taagcttatt gatagtggat acgtctgttt agccggtttg gtcgtcacgg gcgagtggaa
     9361 cttgcctgac aattgcagag gaggtgtgag cgtgtgtctg gtggacaaaa ggatggaaag
     9421 agccgacgag gccattctcg gatcttacta cacagcagct gcaaagaaaa gatttcagtt
     9481 caaggtcgtt cccaattatg ctataaccac ccaggacgcg atgaaaaacg tctggcaagt
     9541 tttagttaat attagaaatg tgaagatgtc agcgggtttc tgtccgcttt ctctggagtt
     9601 tgtgtcggtg tgtattgttt atagaaataa tataaaatta ggtttgagag agaagattac
     9661 aaacgtgaga gacggagggc ccatggaact tacagaagaa gtcgttgatg agttcatgga
     9721 agatgtccct atgtcgatca ggcttgcaaa gtttcgatct cgaaccggaa aaaagagtga
     9781 tgtccgcaaa gggaaaaata gtagtagtga tcggtcagtg ccgaacaaga actatagaaa
     9841 tgttaaggat tttgggggaa tgagttttaa aaagaataat ttaatcgatg atgattcgga
     9901 ggctactgtc gccgaatcgg attcgtttta aatagatctt acagtatcac tactccatct
     9961 cagttcgtgt tcttgtcatt aattaaatgg ctagcaaagg agaagaactt ttcactggag
    10021 ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg
    10081 gagagggtga aggtgatgct acatacggaa agcttacact taaatttatt tgcactactg
    10141 gaaaactacc tgttccatgg ccaacacttg tcactacttt ctcttatggt gttcaatgct
    10201 tttcccgtta tccggatcat atgaaacggc atgacttttt caagagtgcc atgcccgaag
    10261 gttatgtaca ggaacgcact atatctttca aagatgacgg gaactacaag acgcgtgctg
    10321 aagtcaagtt tgaaggtgat acccttgtta atcgtatcga gttaaaaggt attgatttta
    10381 aagaagatgg aaacattctc ggacacaaac tcgagtacaa ctataactca cacaatgtat
    10441 acatcacggc agacaaacaa aagaatggaa tcaaagctaa cttcaaaatt cgccacaaca
    10501 ttgaagatgg atccgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg
    10561 gccctgtcct tttaccagac aaccattacc tgtcgacaca atctgccctt tcgaaagatc
    10621 ccaacgaaaa gcgtgaccac atggtccttc ttgagtttgt aactgctgct gggattacac
    10681 atggcatgga tgagctctac aaataaacta gggcggccgc ggtcctgcaa cttgaggtag
    10741 tcaagatgca taataaataa cggattgtgt ccgtaatcac acgtggtgcg tacgataacg
    10801 catagtgttt ttccctccac ttaaatcgaa gggttgtgtc ttggatcgcg cgggtcaaat
    10861 gtatatggtt catatacatc cgcaggcacg taataaagcg aggggttcga atccccccgt
    10921 tacccccggt aggggcccag gtacccggat gtgttttccg ggctgatgag tccgtgagga
    10981 cgaaacctgg ctgcaggcat gcaagcttgg cgtaatcatg gtcatagcct agagtccgca
    11041 aaaatcacca gtctctctct acaaatctat ctctctctat ttttctccag aataatgtgt
    11101 gagtagttcc cagataaggg aattagggtt cttatagggt ttcgctcatg tgttgagcat
    11161 ataagaaacc cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa
    11221 ttcctaaaac caaaatccag tgacctgcag cccggccggg ggatccacta gcagattgtc
    11281 gtttcccgcc ttcagtttaa actatcagtg tttgacagga tatattggc
//