| 质粒名称: | pJL-TRBO-G |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 80083 |
| 存储实验室: | John Lindbo |
|
载体骨架基本信息 |
|
| 载体名称: | pCB301 |
| 载体出品公司: | -- |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 植物表达 |
| 筛选标记: | 庆大霉素；利福平 |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 30 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | GFP |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pJL-TRBO-G was a gift from John Lindbo (Addgene plasmid # 80083 ; http://n2t.net/addgene:80083 ; RRID:Addgene_80083) |
| 参考文献部分: | TRBO: a high-efficiency tobacco mosaic virus RNA-based overexpression vector. Lindbo JA. Plant Physiol. 2007 Dec;145(4):1232-40. Epub 2007 Aug 24. 10.1104/pp.107.106377 PubMed 17720752 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pJL-TRBO-G 11329 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION An improved agroinfection-compatible Tobacco mosaic virus
(TMV)-based transient expression vector that lacks the TMV CP gene
coding sequence and expresses GFP..
ACCESSION .
VERSION .
KEYWORDS pJL-TRBO-G
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11329)
AUTHORS Lindbo JA
TITLE TRBO: a high-efficiency tobacco mosaic virus RNA-based
overexpression vector.
JOURNAL Plant Physiol. 2007 Dec;145(4):1232-40. Epub 2007 Aug 24.
PUBMED 17720752
REFERENCE 2 (bases 1 to 11329)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11329
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS complement(163..1311)
/codon_start=1
/product="trans-acting replication protein that binds to
and activates oriV"
/label=trfA
/translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
MAQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
CDS complement(1610..2404)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHLERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
rep_origin 2613..3144
/label=oriV
/note="incP origin of replication"
rep_origin 2621..3235
/label=oriV
/note="origin of replication for the bacterial F plasmid"
misc_feature 3328..3352
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
primer_bind 3432..3448
/label=pBluescriptKS
/note="For pBluescript vector"
primer_bind 3433..3449
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 4168..4188
/label=35S promoter
/note="CaMV 35S promoter, forward primer"
promoter 4177..4222
/label=minimal CaMV 35S promoter
/note="minimal 35S promoter from cauliflower mosaic virus"
misc_feature 4234..4289
/label=TMV Omega
/note="translational enhancer from the tobacco mosaic virus
5'-leader sequence (Gallie et al., 1988)"
CDS 9987..10706
/codon_start=1
/product="Cycle 3 GFP (Crameri et al., 1996)"
/label=Cycle 3 GFP
/note="mammalian codon-optimized"
/translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITHGMDELYK"
primer_bind complement(10020..10048)
/label=GFP-R
/note="GFP, reverse primer. Does NOT anneal to EGFP"
primer_bind 10643..10663
/label=GFP-F
/note="GFP, forward primer. Does NOT anneal to EGFP"
misc_feature 11313..8
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
ORIGIN
1 gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa agggcgtgaa
61 aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acaggagatc tcagtaaagc
121 gctggctgct gaacccccag ccggaactga ccccacaagg ccctagcgtt tgcaatgcac
181 caggtcatca ttgacccagg cgtgttccac caggccgctg cctcgcaact cttcgcaggc
241 ttcgccgacc tgctcgcgcc acttcttcac gcgggtggaa tccgatccgc acatgaggcg
301 gaaggtttcc agcttgagcg ggtacggctc ccggtgcgag ctgaaatagt cgaacatccg
361 tcgggccgtc ggcgacagct tgcggtactt ctcccatatg aatttcgtgt agtggtcgcc
421 agcaaacagc acgacgattt cctcgtcgat caggacctgg caacgggacg ttttcttgcc
481 acggtccagg acgcggaagc ggtgcagcag cgacaccgat tccaggtgcc caacgcggtc
541 ggacgtgaag cccatcgccg tcgcctgtag gcgcgacagg cattcctcgg ccttcgtgta
601 ataccggcca ttgatcgacc agcccaggtc ctggcaaagc tcgtagaacg tgaaggtgat
661 cggctcgccg ataggggtgc gcttcgcgta ctccaacacc tgctgccaca ccagttcgtc
721 atcgtcggcc cgcagctcga cgccggtgta ggtgatcttc acgtccttgt tgacgtggaa
781 aatgaccttg ttttgcagcg cctcgcgcgg gattttcttg ttgcgcgtgg tgaacagggc
841 agagcgggcc gtgtcgtttg gcatcgctcg catcgtgtcc ggccacggcg caatatcgaa
901 caaggaaagc tgcatttcct tgatctgctg cttcgtgtgt ttcagcaacg cggcctgctt
961 ggcctcgctg acctgttttg ccaggtcctc gccggcggtt tttcgcttct tggtcgtcat
1021 agttcctcgc gtgtcgatgg tcatcgactt cgccaaacct gccgcctcct gttcgagacg
1081 acgcgaacgc tccacggcgg ccgatggcgc gggcagggca gggggagcca gttgcacgct
1141 gtcgcgctcg atcttggccg tagcttgctg ggccatcgag ccgacggact ggaaggtttc
1201 gcggggcgca cgcatgacgg tgcggcttgc gatggtttcg gcatcctcgg cggaaaaccc
1261 cgcgtcgatc agttcttgcc tgtatgcctt ccggtcaaac gtccgattca ttcaccctcc
1321 ttgcgggatt gccccgactc acgccggggc aatgtgccct tattcctgat ttgacccgcc
1381 tggtgccttg gtgtccagat aatccacctt atcggcaatg aagtcggtcc cgtagaccgt
1441 ctggccgtcc ttctcgtact tggtattccg aatcttgccc tgcacgaata ccagcgaccc
1501 cttgcccaaa tacttgccgt gggcctcggc ctgagagcca aaacacttga tgcggaagaa
1561 gtcggtgcgc tcctgcttgt cgccggcatc gttgcgccac atctaggtac taaaacaatt
1621 catccagtaa aatataatat tttattttct cccaatcagg cttgatcccc agtaagtcaa
1681 aaaatagctc gacatactgt tcttccccga tatcctccct gatcgaccgg acgcagaagg
1741 caatgtcata ccacttgtcc gccctgccgc ttctcccaag atcaataaag ccacttactt
1801 tgccatcttt cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct
1861 cttcgggctt ttccgtcttt aaaaaatcat acagctcgcg cggatcttta aatggggtgt
1921 cttcttccca gttttcgcaa tccacatcgg ccagatcgtt attcagtaag taatccaatt
1981 cggctaagcg gctgtctaag ctattcgtat agggacaatc cgatatgtcg atggagtgaa
2041 agagcctgat gcactccgca tacagctcga taatcttttc agggctttgt tcatcttcat
2101 actcttccga gcaaaggacg ccatcggcct cactcatgag cagattgctc cagccatcat
2161 gccgttcaag gtgcaggacc tttggaacag gcagctttcc ttccagccat agcatcatgt
2221 ccttttcccg ttccacatca taggtggtcc ctttataccg gctgtccgtc atttttaaat
2281 ataggttttc attttctccc accagcttat ataccttagc aggagacatt ccttccgtat
2341 cttttacgca gcggtatttt tcgatcagtt ttttcaattc cggtgatatt ctcattttag
2401 ccatttatta tttccttcct cttttctaca gtatttaaag ataccccaag aagctaatta
2461 taacaagacg aactccaatt cactgttcct tgcattctaa aactttaaat accagaaaac
2521 agctttttca aagttgtttt caaagttggc gtataacata gtatcgacgg agccgatttt
2581 gaaaccacaa ttatgggtga tgctgcaact cgagcgggcc gggagggttc gagaaggggg
2641 ggcacccccc ttcggcgtgc gcggtcacgc gcacagggcg cagccctggt taaaaacaag
2701 gtttataaat attggtttaa aagcaggtta aaagacaggt tagcggtggc cgaaaaacgg
2761 gcggaaaccc ttgcaaatgc tggattttct gcctgtggac agcccctcaa atgtcaatag
2821 gtgcgcccct catctgtcag cactctgccc ctcaagtgtc aaggatcgcg cccctcatct
2881 gtcagtagtc gcgcccctca agtgtcaata ccgcagggca cttatcccca ggcttgtcca
2941 catcatctgt gggaaactcg cgtaaaatca ggcgttttcg ccgatttgcg aggctggcca
3001 gctccacgtc gccggccgaa atcgagcctg cccctcatct gtcaacgccg cgccgggtga
3061 gtcggcccct caagtgtcaa cgtccgcccc tcatctgtca gtgagggcca agttttccgc
3121 gaggtatcca caacgccggc ggccggccgc ggtgtctcgc acacggcttc gacggcgttt
3181 ctggcgcgtt tgcagggcca tagacggccg ccagcccagc ggcgagggca accagcccgg
3241 tgagcgtctc tagtggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg
3301 ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac
3361 gcttagacaa cttaataaca cattgcggac gtttttaaag tactggggtg gttttggtac
3421 cgggcccccc ctcgaggtcg acggtatcga taagcttgat atcgaattcc tgcaggtcaa
3481 catggtggag cacgacacac ttgtctactc caaaaatatc aaagatacag tctcagaaga
3541 ccaaagggca attgagactt ttcaacaaag ggtaatatcc ggaaacctcc tcggattcca
3601 ttgcccagct atctgtcact ttattgtgaa gatagtggaa aaggaaggtg gctcctacaa
3661 atgccatcat tgcgataaag gaaaggccat cgttgaagat gcctctgccg acagtggtcc
3721 caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc
3781 ttcaaagcaa gtggattgat gtgataacat ggtggagcac gacacacttg tctactccaa
3841 aaatatcaaa gatacagtct cagaagacca aagggcaatt gagacttttc aacaaagggt
3901 aatatccgga aacctcctcg gattccattg cccagctatc tgtcacttta ttgtgaagat
3961 agtggaaaag gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggccatcgt
4021 tgaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt
4081 ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac
4141 tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg
4201 aagttcattt catttggaga gggtattttt acaacaatta ccaacaacaa caaacaacag
4261 acaacattac aattactatt tacaattaca atggcataca cacagacagc taccacatca
4321 gctttgctgg acactgtccg aggaaacaac tccttggtca atgatctagc aaagcgtcgt
4381 ctttacgaca cagcggttga agagtttaac gctcgtgacc gcaggcccaa ggtgaacttt
4441 tcaaaagtaa taagcgagga gcagacgctt attgctaccc gggcgtatcc agaattccaa
4501 attacatttt ataacacgca aaatgccgtg cattcgcttg caggtggatt gcgatcttta
4561 gaactggaat atctgatgat gcaaattccc tacggatcat tgacttatga cataggcggg
4621 aattttgcat cgcatctgtt caagggacga gcatatgtac actgctgcat gcccaacctg
4681 gacgttcgag acatcatgcg gcacgaaggc cagaaagaca gtattgaact atacctttct
4741 aggctagaga gaggggggaa aacagtcccc aacttccaaa aggaagcatt tgacagatac
4801 gcagaaattc ctgaagacgc tgtctgtcac aatactttcc agacatgcga acatcagccg
4861 atgcagcaat caggcagagt gtatgccatt gcgctacaca gcatatatga cataccagcc
4921 gatgagttcg gggcggcact cttgaggaaa aatgtccata cgtgctatgc cgctttccac
4981 ttctccgaga acctgcttct tgaagattca tgcgtcaatt tggacgaaat caacgcgtgt
5041 ttttcgcgcg atggagacaa gttgaccttt tcttttgcat cagagagtac tcttaattac
5101 tgtcatagtt attctaatat tcttaagtat gtgtgcaaaa cttacttccc ggcctctaat
5161 agagaggttt acatgaagga gtttttagtc accagagtta atacctggtt ttgtaagttt
5221 tctagaatag atacttttct tttgtacaaa ggtgtggccc ataaaagtgt agatagtgag
5281 cagttttata ctgcaatgga agacgcatgg cattacaaaa agactcttgc aatgtgcaac
5341 agcgagagaa tcctccttga ggattcatca tcagtcaatt actggtttcc caaaatgagg
5401 gatatggtca tcgtaccatt attcgacatt tctttggaga ctagtaagag gacgcgcaag
5461 gaagtcttag tgtccaagga tttcgtgttc acagtgctta accacattcg aacataccag
5521 gcgaaagctc ttacatacgc aaatgttttg tccttcgtcg aatcgattcg atcgagggta
5581 atcattaacg gtgtgacagc gaggtccgaa tgggatgtgg acaaatcttt gttacaatcc
5641 ttgtccatga cgttttacct gcatactaag cttgccgttc taaaggatga cttactgatt
5701 agcaagttta gtctcggttc gaaaacggtg tgccagcatg tgtgggatga gatttcgctg
5761 gcgtttggga acgcatttcc ctccgtgaaa gagaggctct tgaacaggaa acttatcaga
5821 gtggcaggcg acgcattaga gatcagggtg cctgatctat atgtgacctt ccacgacaga
5881 ttagtgactg agtacaaggc ctctgtggac atgcctgcgc ttgacattag gaagaagatg
5941 gaagaaacgg aagtgatgta caatgcactt tcagaattat cggtgttaag ggagtctgac
6001 aaattcgatg ttgatgtttt ttcccagatg tgccaatctt tggaagttga cccaatgacg
6061 gcagcgaagg ttatagtcgc ggtcatgagc aatgagagcg gtctgactct cacatttgaa
6121 cgacctactg aggcgaatgt tgcgctagct ttacaggatc aagagaaggc ttcagaaggt
6181 gcattggtag ttacctcaag agaagttgaa gaaccgtcca tgaagggttc gatggccaga
6241 ggagagttac aattagctgg tcttgctgga gatcatccgg aatcgtccta ttctaagaac
6301 gaggagatag agtctttaga gcagtttcat atggcgacgg cagattcgtt aattcgtaag
6361 cagatgagct cgattgtgta cacgggtccg attaaagttc agcaaatgaa aaactttatc
6421 gatagcctgg tagcatcact atctgctgcg gtgtcgaatc tcgtcaagat cctcaaagat
6481 acagctgcta ttgaccttga aacccgtcaa aagtttggag tcttggatgt tgcatctagg
6541 aagtggttaa tcaaaccaac ggccaagagt catgcatggg gtgttgttga aacccacgcg
6601 aggaagtatc atgtggcgct tttggaatat gatgagcagg gtgtggtgac atgcgatgat
6661 tggagaagag tagctgttag ctctgagtct gttgtttatt ccgacatggc gaaactcaga
6721 actctgcgca gactgcttcg aaacggagaa ccgcatgtca gtagcgcaaa ggttgttctt
6781 gtggacggag ttccgggctg tggaaaaacc aaagaaattc tttccagggt taattttgat
6841 gaagatctaa ttttagtacc tgggaagcaa gccgcggaaa tgatcagaag acgtgcgaat
6901 tcctcaggga ttattgtggc cacgaaggac aacgttaaaa ccgttgattc tttcatgatg
6961 aattttggga aaagcacacg ctgtcagttc aagaggttat tcattgatga agggttgatg
7021 ttgcatactg gttgtgttaa ttttcttgtg gcgatgtcat tgtgcgaaat tgcatatgtt
7081 tacggagaca cacagcagat tccatacatc aatagagttt caggattccc gtaccccgcc
7141 cattttgcca aattggaagt tgacgaggtg gagacacgca gaactactct ccgttgtcca
7201 gccgatgtca cacattatct gaacaggaga tatgagggct ttgtcatgag cacttcttcg
7261 gttaaaaagt ctgtttcgca ggagatggtc ggcggagccg ccgtgatcaa tccgatctca
7321 aaacccttgc atggcaagat cctgactttt acccaatcgg ataaagaagc tctgctttca
7381 agagggtatt cagatgttca cactgtgcat gaagtgcaag gcgagacata ctctgatgtt
7441 tcactagtta ggttaacccc tacaccggtc tccatcattg caggagacag cccacatgtt
7501 ttggtcgcat tgtcaaggca cacctgttcg ctcaagtact acactgttgt tatggatcct
7561 ttagttagta tcattagaga tctagagaaa cttagctcgt acttgttaga tatgtataag
7621 gtcgatgcag gaacacaata gcaattacag attgactcgg tgttcaaagg ttccaatctt
7681 tttgttgcag cgccaaagac tggtgatatt tctgatatgc agttttacta tgataagtgt
7741 ctcccaggca acagcaccat gatgaataat tttgatgctg ttaccatgag gttgactgac
7801 atttcattga atgtcaaaga ttgcatattg gatatgtcta agtctgttgc tgcgcctaag
7861 gatcaaatca aaccactaat acctatggta cgaacggcgg cagaaatgcc acgccagact
7921 ggactattgg aaaatttagt ggcgatgatt aaaagaaact ttaacgcacc cgagttgtct
7981 ggcatcattg atattgaaaa tactgcatct ttggttgtag ataagttttt tgatagttat
8041 ttgcttaaag aaaaaagaaa accaaataaa aatgtttctt tgttcagtag agagtctctc
8101 aatagatggt tagaaaagca ggaacaggta acaataggcc agctcgcaga ttttgatttt
8161 gtggatttgc cagcagttga tcagtacaga cacatgatta aagcacaacc caaacaaaag
8221 ttggacactt caatccaaac ggagtacccg gctttgcaga cgattgtgta ccattcaaaa
8281 aagatcaatg caatattcgg cccgttgttt agtgagctta ccaggcaatt actggacagt
8341 gttgattcga gcagattttt gtttttcaca agaaagacac cagcgcagat tgaggatttc
8401 ttcggagatc tcgacagtca tgtgccgatg gatgtcttgg agctggatat atcaaaatac
8461 gacaaatctc agaatgaatt ccactgtgca gtagaatacg agatctggcg aagattgggt
8521 ttcgaagact tcttgggaga agtttggaaa caagggcata gaaagaccac cctcaaggat
8581 tataccgcag gtataaaaac ttgcatctgg tatcaaagaa agagcgggga cgtcacgacg
8641 ttcattggaa acactgtgat cattgctgca tgtttggcct cgatgcttcc gatggagaaa
8701 ataatcaaag gagccttttg cggtgacgat agtctgctgt actttccaaa gggttgtgag
8761 tttccggatg tgcaacactc cgcgaatctt atgtggaatt ttgaagcaaa actgtttaaa
8821 aaacagtatg gatacttttg cggaagatat gtaatacatc acgacagagg atgcattgtg
8881 tattacgatc ccctaaagtt gatctcgaaa cttggtgcta aacacatcaa ggattgggaa
8941 cacttggagg agttcagaag gtctctttgt gatgttgctg tttcgttgaa caattgtgcg
9001 tattacacac agttggacga cgctgtatgg gaggttcata agaccgcccc tccaggttcg
9061 tttgtttata aaagtctggt gaagtatttg tctgataaag ttctttttag aagtttgttt
9121 atagatggct ctagttgtta aaggaaaagt gaatatcaat gagtttatcg acctgacaaa
9181 aatggagaag atcttaccgt cgatgtttac ccctgtaaag agtgttatgt gttccaaagt
9241 tgataaaata atggttcatg agaatgagtc attgtcaggg gtgaaccttc ttaaaggagt
9301 taagcttatt gatagtggat acgtctgttt agccggtttg gtcgtcacgg gcgagtggaa
9361 cttgcctgac aattgcagag gaggtgtgag cgtgtgtctg gtggacaaaa ggatggaaag
9421 agccgacgag gccattctcg gatcttacta cacagcagct gcaaagaaaa gatttcagtt
9481 caaggtcgtt cccaattatg ctataaccac ccaggacgcg atgaaaaacg tctggcaagt
9541 tttagttaat attagaaatg tgaagatgtc agcgggtttc tgtccgcttt ctctggagtt
9601 tgtgtcggtg tgtattgttt atagaaataa tataaaatta ggtttgagag agaagattac
9661 aaacgtgaga gacggagggc ccatggaact tacagaagaa gtcgttgatg agttcatgga
9721 agatgtccct atgtcgatca ggcttgcaaa gtttcgatct cgaaccggaa aaaagagtga
9781 tgtccgcaaa gggaaaaata gtagtagtga tcggtcagtg ccgaacaaga actatagaaa
9841 tgttaaggat tttgggggaa tgagttttaa aaagaataat ttaatcgatg atgattcgga
9901 ggctactgtc gccgaatcgg attcgtttta aatagatctt acagtatcac tactccatct
9961 cagttcgtgt tcttgtcatt aattaaatgg ctagcaaagg agaagaactt ttcactggag
10021 ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg
10081 gagagggtga aggtgatgct acatacggaa agcttacact taaatttatt tgcactactg
10141 gaaaactacc tgttccatgg ccaacacttg tcactacttt ctcttatggt gttcaatgct
10201 tttcccgtta tccggatcat atgaaacggc atgacttttt caagagtgcc atgcccgaag
10261 gttatgtaca ggaacgcact atatctttca aagatgacgg gaactacaag acgcgtgctg
10321 aagtcaagtt tgaaggtgat acccttgtta atcgtatcga gttaaaaggt attgatttta
10381 aagaagatgg aaacattctc ggacacaaac tcgagtacaa ctataactca cacaatgtat
10441 acatcacggc agacaaacaa aagaatggaa tcaaagctaa cttcaaaatt cgccacaaca
10501 ttgaagatgg atccgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg
10561 gccctgtcct tttaccagac aaccattacc tgtcgacaca atctgccctt tcgaaagatc
10621 ccaacgaaaa gcgtgaccac atggtccttc ttgagtttgt aactgctgct gggattacac
10681 atggcatgga tgagctctac aaataaacta gggcggccgc ggtcctgcaa cttgaggtag
10741 tcaagatgca taataaataa cggattgtgt ccgtaatcac acgtggtgcg tacgataacg
10801 catagtgttt ttccctccac ttaaatcgaa gggttgtgtc ttggatcgcg cgggtcaaat
10861 gtatatggtt catatacatc cgcaggcacg taataaagcg aggggttcga atccccccgt
10921 tacccccggt aggggcccag gtacccggat gtgttttccg ggctgatgag tccgtgagga
10981 cgaaacctgg ctgcaggcat gcaagcttgg cgtaatcatg gtcatagcct agagtccgca
11041 aaaatcacca gtctctctct acaaatctat ctctctctat ttttctccag aataatgtgt
11101 gagtagttcc cagataaggg aattagggtt cttatagggt ttcgctcatg tgttgagcat
11161 ataagaaacc cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa
11221 ttcctaaaac caaaatccag tgacctgcag cccggccggg ggatccacta gcagattgtc
11281 gtttcccgcc ttcagtttaa actatcagtg tttgacagga tatattggc
//
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