pGL2-Basic

价格:1500元

联系方式:I47-825O-882O

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pGL2Basic载体基本信息

出品公司: Promega
载体名称: pGL2-Basic
质粒类型: 荧光素酶载体
高拷贝/低拷贝: 高拷贝
克隆方法: --
启动子: --
载体大小: 5598 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素
筛选标记: --
克隆菌株: --
宿主细胞(系): --
备注:
pGL2-Basic没有增强子或启动子。宿主:大肠杆菌JM109,哺乳动物。相关载体:f1,pBR322,pGL2-Enhancer, pGL2-Promoter,pGL2-Control。(信息来源:VectorDB(http://seq.yeastgome.org/VectorDB.))无启动子载体,用于用荧光素酶测定测定启动子和增强子序列的活性。
产品目录号: --
稳定性: --
组成型/诱导型: --
病毒/非病毒: --

pGL2Basic载体质粒图谱和多克隆位点信息

pGL2-Basic载体图谱

pGL2Basic载体简介

pGL2Basic载体序列

LOCUS       pGL2-Basic                5598 bp ds-DNA     circular SYN 28-JUN-2013
DEFINITION  Promoterless vector for measuring the activity of promoter and 
            enhancer sequences with a luciferase assay.
ACCESSION   X65323
VERSION     .
KEYWORDS    pGL2-Basic
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5598)
  AUTHORS   Promega
  TITLE     Direct Submission
  JOURNAL   Exported Monday, February 3, 2020 from SnapGene Viewer 3.1.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5598
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
     misc_feature    1..52
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             76..1728
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /note="luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     intron          1969..2034
                     /note="small t intron"
                     /note="simian virus 40 (SV40) small t antigen intron"
     polyA_signal    2609..2743
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3056..3644)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3815..4675)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4676..4780)
                     /gene="bla"
                     /note="AmpR promoter"
     rep_origin      4807..5262
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     polyA_signal    5452..5573
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 cccgggaggt accgagctct tacgcgtgct agctcgagat ctaagtaagc ttggcattcc
       61 ggtactgttg gtaaaatgga agacgccaaa aacataaaga aaggcccggc gccattctat
      121 cctctagagg atggaaccgc tggagagcaa ctgcataagg ctatgaagag atacgccctg
      181 gttcctggaa caattgcttt tacagatgca catatcgagg tgaacatcac gtacgcggaa
      241 tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac gatatgggct gaatacaaat
      301 cacagaatcg tcgtatgcag tgaaaactct cttcaattct ttatgccggt gttgggcgcg
      361 ttatttatcg gagttgcagt tgcgcccgcg aacgacattt ataatgaacg tgaattgctc
      421 aacagtatga acatttcgca gcctaccgta gtgtttgttt ccaaaaaggg gttgcaaaaa
      481 attttgaacg tgcaaaaaaa attaccaata atccagaaaa ttattatcat ggattctaaa
      541 acggattacc agggatttca gtcgatgtac acgttcgtca catctcatct acctcccggt
      601 tttaatgaat acgattttgt accagagtcc tttgatcgtg acaaaacaat tgcactgata
      661 atgaattcct ctggatctac tgggttacct aagggtgtgg cccttccgca tagaactgcc
      721 tgcgtcagat tctcgcatgc cagagatcct atttttggca atcaaatcat tccggatact
      781 gcgattttaa gtgttgttcc attccatcac ggttttggaa tgtttactac actcggatat
      841 ttgatatgtg gatttcgagt cgtcttaatg tatagatttg aagaagagct gtttttacga
      901 tcccttcagg attacaaaat tcaaagtgcg ttgctagtac caaccctatt ttcattcttc
      961 gccaaaagca ctctgattga caaatacgat ttatctaatt tacacgaaat tgcttctggg
     1021 ggcgcacctc tttcgaaaga agtcggggaa gcggttgcaa aacgcttcca tcttccaggg
     1081 atacgacaag gatatgggct cactgagact acatcagcta ttctgattac acccgagggg
     1141 gatgataaac cgggcgcggt cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat
     1201 ctggataccg ggaaaacgct gggcgttaat cagagaggcg aattatgtgt cagaggacct
     1261 atgattatgt ccggttatgt aaacaatccg gaagcgacca acgccttgat tgacaaggat
     1321 ggatggctac attctggaga catagcttac tgggacgaag acgaacactt cttcatagtt
     1381 gaccgcttga agtctttaat taaatacaaa ggatatcagg tggcccccgc tgaattggaa
     1441 tcgatattgt tacaacaccc caacatcttc gacgcgggcg tggcaggtct tcccgacgat
     1501 gacgccggtg aacttcccgc cgccgttgtt gttttggagc acggaaagac gatgacggaa
     1561 aaagagatcg tggattacgt cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga
     1621 gttgtgtttg tggacgaagt accgaaaggt cttaccggaa aactcgacgc aagaaaaatc
     1681 agagagatcc tcataaaggc caagaagggc ggaaagtcca aattgtaaaa tgtaactgta
     1741 ttcagcgatg acgaaattct tagctattgt aatactgcga tgagtggcag ggcggggcgt
     1801 aattttttta aggcagttat tggtgccctt aaacgcctgg ttgctacgcc tgaataagtg
     1861 ataataagcg gatgaatggc agaaattcgc cggatctttg tgaaggaacc ttacttctgt
     1921 ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa
     1981 tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca
     2041 acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt
     2101 tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact
     2161 cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt
     2221 tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca
     2281 aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatattctgt aacctttata
     2341 agtaggcata acagttataa tcataacata ctgttttttc ttactccaca caggcataga
     2401 gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa
     2461 ggggttaata aggaatattt gatgtatagt gccttgacta gagatcataa tcagccatac
     2521 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     2581 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     2641 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     2701 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccgtcgac cgatgccctt
     2761 gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc
     2821 acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctcttccg
     2881 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     2941 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     3001 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     3061 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     3121 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     3181 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     3241 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     3301 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     3361 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     3421 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     3481 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     3541 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     3601 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     3661 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     3721 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     3781 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     3841 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     3901 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     3961 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     4021 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     4081 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     4141 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     4201 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     4261 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     4321 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     4381 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     4441 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     4501 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     4561 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     4621 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     4681 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     4741 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     4801 cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     4861 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     4921 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     4981 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     5041 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     5101 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     5161 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     5221 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttgccattcg ccattcaggc
     5281 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagcccaagc
     5341 taccatgata agtaagtaat attaaggtac gtggaggttt tacttgcttt aaaaaacctc
     5401 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     5461 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     5521 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatggtact
     5581 gtaactgagc taacataa
//

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