| 质粒名称: | pMCSG7-Wt-NmeCas9 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 71474 |
| 存储实验室: | Erik Sontheimer |
|
载体骨架基本信息 |
|
| 载体名称: | pMCSG7 |
| 载体出品公司: | MCSG |
| 空载体大小: | 5286 bp |
| 完整质粒大小: | 8541 bp |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | -- |
|
插入基因信息 |
|
| 插入基因名称: | pMCSG7-WT-NmeCas9 |
| 物种来源: | Neisseria meningitidis |
| 插入大小: | 3321 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | 6His（插入件上的N端子） |
| 克隆方法: | -- |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pMCSG7-Wt-NmeCas9 was a gift from Erik Sontheimer (Addgene plasmid # 71474 ; http://n2t.net/addgene:71474 ; RRID:Addgene_71474) |
| 参考文献部分: | DNase H Activity of Neisseria meningitidis Cas9. Zhang Y, Rajan R, Seifert HS, Mondragon A, Sontheimer EJ. Mol Cell. 2015 Oct 15;60(2):242-55. doi: 10.1016/j.molcel.2015.09.020. 10.1016/j.molcel.2015.09.020 PubMed 26474066 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pMCSG7-Wt-NmeCas9 8541 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION bacterial pMCSG7 expression vector expressing Wildtype Nme cas9 with
T7 promoter, N-terminal His tag and TEV site.
ACCESSION .
VERSION .
KEYWORDS pMCSG7-Wt-NmeCas9
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8541)
AUTHORS Zhang Y, Rajan R, Seifert HS, Mondragon A, Sontheimer EJ
TITLE DNase H Activity of Neisseria meningitidis Cas9.
JOURNAL Mol Cell. 2015 Oct 15;60(2):242-55. doi:
10.1016/j.molcel.2015.09.020.
PUBMED 26474066
REFERENCE 2 (bases 1 to 8541)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8541
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(99..118)
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 285..388
/gene="bla"
/label=AmpR promoter
CDS 389..1249
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(607..626)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 1420..2008
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 1909..1928
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 2162..2179
/label=L4440
/note="L4440 vector, forward primer"
misc_feature 2194..2336
/label=bom
/note="basis of mobility region from pBR322"
primer_bind complement(2262..2281)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 2422..2444
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
CDS complement(2438..2629)
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/label=rop
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
CDS complement(3438..4520)
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
primer_bind 4482..4501
/label=LacI-R
/note="LacI, reverse primer"
promoter complement(4521..4598)
/gene="lacI"
/label=lacI promoter
primer_bind 4804..4823
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind 4911..4930
/label=T7
/note="T7 promoter, forward primer"
promoter 4911..4929
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 4930..4954
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 4969..4991
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 5002..5019
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
CDS 5044..5064
/codon_start=1
/product="tobacco etch virus (TEV) protease recognition and
cleavage site"
/label=TEV site
/translation="ENLYFQS"
CDS 5071..8319
/codon_start=1
/product="Cas9 endonuclease from the Neisseria meningitidis
Type II CRISPR/Cas system (Esvelt et al., 2013)"
/label=NmCas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="MAAFKPNSINYILGLDIGIASVGWAMVEIDEEENPIRLIDLGVRV
FERAEVPKTGDSLAMARRLARSVRRLTRRRAHRLLRTRRLLKREGVLQAANFDENGLIK
SLPNTPWQLRAAALDRKLTPLEWSAVLLHLIKHRGYLSQRKNEGETADKELGALLKGVA
GNAHALQTGDFRTPAELALNKFEKESGHIRNQRSDYSHTFSRKDLQAELILLFEKQKEF
GNPHVSGGLKEGIETLLMTQRPALSGDAVQKMLGHCTFEPAEPKAAKNTYTAERFIWLT
KLNNLRILEQGSERPLTDTERATLMDEPYRKSKLTYAQARKLLGLEDTAFFKGLRYGKD
NAEASTLMEMKAYHAISRALEKEGLKDKKSPLNLSPELQDEIGTAFSLFKTDEDITGRL
KDRIQPEILEALLKHISFDKFVQISLKALRRIVPLMEQGKRYDEACAEIYGDHYGKKNT
EEKIYLPPIPADEIRNPVVLRALSQARKVINGVVRRYGSPARIHIETAREVGKSFKDRK
EIEKRQEENRKDREKAAAKFREYFPNFVGEPKSKDILKLRLYEQQHGKCLYSGKEINLG
RLNEKGYVEIDHALPFSRTWDDSFNNKVLVLGSENQNKGNQTPYEYFNGKDNSREWQEF
KARVETSRFPRSKKQRILLQKFDEDGFKERNLNDTRYVNRFLCQFVADRMRLTGKGKKR
VFASNGQITNLLRGFWGLRKVRAENDRHHALDAVVVACSTVAMQQKITRFVRYKEMNAF
DGKTIDKETGEVLHQKTHFPQPWEFFAQEVMIRVFGKPDGKPEFEEADTLEKLRTLLAE
KLSSRPEAVHEYVTPLFVSRAPNRKMSGQGHMETVKSAKRLDEGVSVLRVPLTQLKLKD
LEKMVNREREPKLYEALKARLEAHKDDPAKAFAEPFYKYDKAGNRTQQVKAVRVEQVQK
TGVWVRNHNGIADNATMVRVDVFEKGDKYYLVPIYSWQVAKGILPDRAVVQGKDEEDWQ
LIDDSFNFKFSLHPNDLVEVITKKARMFGYFASCHRGTGNINIRIHDLDHKIGKNGILE
GIGVKTALSFQKYQIDELGKEIRPCRLKKRPPVR"
CDS 8385..8402
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(8455..8473)
/label=T7 Term
/note="T7 terminator, reverse primer"
terminator 8469..8516
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
ORIGIN
1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
241 acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
301 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
361 tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
421 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
481 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
541 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
601 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
661 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
721 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
781 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
841 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
901 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
961 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
1021 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
1081 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
1141 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
1201 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
1261 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
1321 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
1381 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
1441 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
1501 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
1561 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
1621 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
1681 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
1741 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
1801 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
1861 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
1921 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
1981 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
2041 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
2101 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
2161 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
2221 tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc
2281 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
2341 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
2401 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
2461 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga
2521 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc
2581 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg
2641 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca
2701 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac
2761 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg
2821 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga
2881 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga
2941 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc
3001 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg
3061 tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg
3121 cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt
3181 gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt
3241 cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga
3301 agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg
3361 ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta
3421 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
3481 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt
3541 tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag
3601 agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg
3661 gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata
3721 tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga
3781 tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt
3841 tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg
3901 cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg
3961 cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc
4021 gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga
4081 aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc
4141 ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta
4201 caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg
4261 gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg
4321 gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg
4381 taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg
4441 gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg
4501 tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat
4561 gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt
4621 atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc
4681 cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc
4741 caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc
4801 atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc
4861 cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact
4921 cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt
4981 taagaaggag atatacatat gcaccatcat catcatcatt cttctggtgt agatctgggt
5041 accgagaacc tgtacttcca atccaatgcc atggctgcct tcaaacctaa ttcaatcaac
5101 tacatcctcg gcctcgatat cggcatcgca tccgtcggct gggcgatggt agaaattgac
5161 gaagaagaaa accccatccg cctgattgat ttgggcgtgc gcgtatttga gcgtgccgaa
5221 gtaccgaaaa caggcgactc ccttgccatg gcaaggcgtt tggcgcgcag tgttcgccgc
5281 ctgacccgcc gtcgcgccca ccgcctgctt cggacccgcc gcctattgaa acgcgaaggc
5341 gtattacaag ccgccaattt tgacgaaaac ggcttgatta aatccttacc gaatacacca
5401 tggcaacttc gcgcagccgc attagaccgc aaactgacgc ctttagagtg gtcggcagtc
5461 ttgttgcatt taatcaaaca tcgcggctat ttatcgcaac ggaaaaacga gggcgaaact
5521 gccgataagg agcttggcgc tttgcttaaa ggcgtagccg gcaatgccca tgccttacag
5581 acaggcgatt tccgcacacc ggccgaattg gctttaaata aatttgagaa agaaagcggc
5641 catatccgca atcagcgcag cgattattcg catacgttca gccgcaaaga tttacaggcg
5701 gagctgattt tgctgtttga aaaacaaaaa gaatttggca atccgcatgt ttcaggcggc
5761 cttaaagaag gtattgaaac cctactgatg acgcaacgcc ctgccctgtc cggcgatgcc
5821 gttcaaaaaa tgttggggca ttgcaccttc gaaccggcag agccgaaagc cgctaaaaac
5881 acctacacag ccgaacgttt catctggctg accaagctga acaacctgcg tattttagag
5941 caaggcagcg agcggccatt gaccgatacc gaacgcgcca cgcttatgga cgagccatac
6001 agaaaatcca aactgactta cgcacaagcc cgtaagctgc tgggtttaga agataccgcc
6061 tttttcaaag gcttgcgcta tggtaaagac aatgccgaag cctcaacatt gatggaaatg
6121 aaggcctacc atgccatcag ccgtgcactg gaaaaagaag gattgaaaga caaaaaatcc
6181 ccattaaacc tttctcccga attacaagac gaaatcggca cggcattctc cctgttcaaa
6241 accgatgaag acattacagg ccgtctgaaa gaccgtatac agcccgaaat cttagaagcg
6301 ctgttgaaac acatcagctt cgataagttc gtccaaattt ccttgaaagc attgcgccga
6361 attgtgcctc taatggaaca aggcaaacgt tacgatgaag cctgcgccga aatctacgga
6421 gaccattacg gcaagaagaa tacggaagaa aagatttatc tgccgccgat tcccgccgac
6481 gaaatccgca accccgtcgt cttgcgcgcc ttatctcaag cacgtaaggt cattaacggc
6541 gtggtacgcc gttacggctc cccagctcgt atccatattg aaactgcaag ggaagtaggt
6601 aaatcgttta aagaccgcaa agaaattgag aaacgccaag aagaaaaccg caaagaccgg
6661 gaaaaagccg ccgccaaatt ccgagagtat ttccccaatt ttgtcggaga acccaaatcc
6721 aaagatattc tgaaactgcg cctgtacgag caacaacacg gcaaatgcct gtattcgggc
6781 aaagaaatca acttaggccg tctgaacgaa aaaggctatg tcgaaatcga ccatgccctg
6841 ccgttctcgc gcacatggga cgacagtttc aacaataaag tactggtatt gggcagcgaa
6901 aaccaaaaca aaggcaatca aaccccttac gaatacttca acggcaaaga caacagccgc
6961 gaatggcagg aatttaaagc gcgtgtcgaa accagccgtt tcccgcgcag taaaaaacaa
7021 cggattctgc tgcaaaaatt cgatgaagac ggctttaaag aacgcaatct gaacgacacg
7081 cgctacgtca accgtttcct gtgtcaattt gttgccgacc gtatgcggct gacaggtaaa
7141 ggcaagaaac gtgtctttgc atccaacgga caaattacca atctgttgcg cggcttttgg
7201 ggattgcgca aagtgcgtgc ggaaaacgac cgccatcacg ccttggacgc cgtcgtcgtt
7261 gcctgctcga ccgttgccat gcagcagaaa attacccgtt ttgtacgcta taaagagatg
7321 aacgcgtttg acggtaaaac catagacaaa gaaacaggag aagtgctgca tcaaaaaaca
7381 cacttcccac aaccttggga atttttcgca caagaagtca tgattcgcgt cttcggcaaa
7441 ccggacggca aacccgaatt cgaagaagcc gataccctag aaaaactgcg cacgttgctt
7501 gccgaaaaat tatcatctcg ccccgaagcc gtacacgaat acgttacgcc actgtttgtt
7561 tcacgcgcgc ccaatcggaa gatgagcggg caagggcata tggagaccgt caaatccgcc
7621 aaacgactgg acgaaggcgt cagcgtgttg cgcgtaccgc tgacacagtt aaaactgaaa
7681 gacttggaaa aaatggtcaa tcgggagcgc gaacctaagc tatacgaagc actgaaagca
7741 cggctggaag cacataaaga cgatcctgcc aaagcctttg ccgagccgtt ttacaaatac
7801 gataaagcag gcaaccgcac ccaacaggta aaagccgtac gcgtagagca agtacagaaa
7861 accggcgtat gggtgcgcaa ccataacggt attgccgaca acgcaaccat ggtgcgcgta
7921 gatgtgtttg agaaaggcga caagtattat ctggtaccga tttacagttg gcaggtagcg
7981 aaagggattt tgccggatag ggctgttgta caaggaaaag atgaagaaga ttggcaactt
8041 attgatgata gtttcaactt taaattctca ttacacccta atgatttagt cgaggttata
8101 acaaaaaaag ctagaatgtt tggttacttt gccagctgcc atcgaggcac aggtaatatc
8161 aatatacgca ttcatgatct tgatcataaa attggcaaaa atggaatact ggaaggtatc
8221 ggcgtcaaaa ccgccctttc attccaaaaa taccaaattg acgaactggg caaagaaatc
8281 agaccatgcc gtctgaaaaa acgcccgcct gtccgttaaa acattggaag tggataacgg
8341 atccgaattc gagctccgtc gacaagcttg cggccgcact cgagcaccac caccaccacc
8401 actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
8461 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
8521 aaggaggaac tatatccgga t
//
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