| 质粒名称: | CAPTURE2_pLVX-EF1a-dCas9-CBio-IRES-zsGreen1 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 138418 |
| 存储实验室: | Jian Xu |
|
载体骨架基本信息 |
|
| 载体名称: | pLVX-EF1a-zsGreen1 |
| 载体出品公司: | Takara Bio |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 慢病毒载体 |
| 筛选标记: | zsGreen1 |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | dCas9 |
| 物种来源: | Streptococcus pyogenes |
| 插入大小: | 4401 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | EF1a |
| 标签/融合蛋白: | BioTAP-tag（插接件上的C端子） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | CAPTURE2_pLVX-EF1a-dCas9-CBio-IRES-zsGreen1 was a gift from Jian Xu (Addgene plasmid # 138418 ; http://n2t.net/addgene:138418 ; RRID:Addgene_138418) |
| 参考文献部分: | Multiplexed capture of spatial configuration and temporal dynamics of locus-specific 3D chromatin by biotinylated dCas9. Liu X, Chen Y, Zhang Y, Liu Y, Liu N, Botten GA, Cao H, Orkin SH, Zhang MQ, Xu J. Genome Biol. 2020 Mar 5;21(1):59. doi: 10.1186/s13059-020-01973-w. 10.1186/s13059-020-01973-w PubMed 32138752 |
买家导航
CAPTURE2_pLVX-EF1a-dCas9-CBio-IRES-zsGreen1载体质粒基本信息
CAPTURE2_pLVX-EF1a-dCas9-CBio-IRES-zsGreen1质粒图谱载体图谱和CAPTURE2_pLVX-EF1a-dCas9-CBio-IRES-zsGreen1载体序列质粒序列多克隆位点信息
CAPTURE2_pLVX-EF1a-dCas9-CBio-IRES-zsGreen1质粒载体简介
CAPTURE2_pLVX-EF1a-dCas9-CBio-IRES-zsGreen1质粒序列载体序列
LOCUS CAPTURE2_pLVX-EF1a-dCas9-CBio-IRES-zsGreen1 13286 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION CAPTURE2.0 vector containing dCas9-CBio, IRES and zsGreen1.
ACCESSION .
VERSION .
KEYWORDS CAPTURE2_pLVX-EF1a-dCas9-CBio-IRES-zsGreen1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13286)
AUTHORS Liu X, Chen Y, Zhang Y, Liu Y, Liu N, Botten GA, Cao H, Orkin SH,
Zhang MQ, Xu J
TITLE Multiplexed capture of spatial configuration and temporal dynamics
of locus-specific 3D chromatin by biotinylated dCas9.
JOURNAL Genome Biol. 2020 Mar 5;21(1):59. doi: 10.1186/s13059-020-01973-w.
PUBMED 32138752
REFERENCE 2 (bases 1 to 13286)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..13286
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..634
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 681..806
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1303..1536
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1721..1765
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2028..2143
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1 (lacking the first T)"
promoter 2338..3519
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 2568..3510
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 3467..3487
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
regulatory 3558..3567
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 3573..3593
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 3594..7694
/codon_start=1
/product="catalytically dead mutant of the Cas9
endonuclease from the Streptococcus pyogenes Type II
CRISPR/Cas system"
/label=dCas9
/note="RNA-guided DNA-binding protein that lacks
endonuclease activity due to the D10A mutation in the RuvC
catalytic domain and the H840A mutation in the HNH
catalytic domain"
/translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 7704..7724
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
misc_feature 7972..8544
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
misc_feature 7995..8546
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(8139..8156)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
CDS 8546..9241
/codon_start=1
/product="Zoanthus green fluorescent protein"
/label=ZsGreen1
/note="mammalian codon-optimized"
/translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
AIASGSALP"
misc_feature 9255..9843
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(9308..9328)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(9726..9737)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 10050..10683
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
primer_bind complement(10811..10827)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(10811..10827)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(10824..10846)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 10835..10851
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(10859..10889)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 10904..10925
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(11042..11059)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(11213..11801)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(11293..11312)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(11972..12832)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 12595..12614
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(12833..12937)
/gene="bla"
/label=AmpR promoter
polyA_signal 12985..13119
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(13022..13041)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 13076..13095
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg
2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc
2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct
2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatctatttc cggtgaattc
3541 ctcgagacta gttctaggcc accatggcta gccccaaaaa gaagaggaaa gtggacaaga
3601 agtattctat cggactggcc atcgggacta atagcgtcgg gtgggccgtg atcactgacg
3661 agtacaaggt gccctctaag aagttcaagg tgctcgggaa caccgaccgg cattccatca
3721 agaaaaatct gatcggagct ctcctctttg attcagggga gaccgctgaa gcaacccgcc
3781 tcaagcggac tgctagacgg cggtacacca ggaggaagaa ccggatttgt taccttcaag
3841 agatattctc caacgaaatg gcaaaggtcg acgacagctt cttccatagg ctggaagaat
3901 cattcctcgt ggaagaggat aagaagcatg aacggcatcc catcttcggt aatatcgtcg
3961 acgaggtggc ctatcacgag aaatacccaa ccatctacca tcttcgcaaa aagctggtgg
4021 actcaaccga caaggcagac ctccggctta tctacctggc cctggcccac atgatcaagt
4081 tcagaggcca cttcctgatc gagggcgacc tcaatcctga caatagcgat gtggataaac
4141 tgttcatcca gctggtgcag acttacaacc agctctttga agagaacccc atcaatgcaa
4201 gcggagtcga tgccaaggcc attctgtcag cccggctgtc aaagagccgc agacttgaga
4261 atcttatcgc tcagctgccg ggtgaaaaga aaaatggact gttcgggaac ctgattgctc
4321 tttcacttgg gctgactccc aatttcaagt ctaatttcga cctggcagag gatgccaagc
4381 tgcaactgtc caaggacacc tatgatgacg atctcgacaa cctcctggcc cagatcggtg
4441 accaatacgc cgaccttttc cttgctgcta agaatctttc tgacgccatc ctgctgtctg
4501 acattctccg cgtgaacact gaaatcacca aggcccctct ttcagcttca atgattaagc
4561 ggtatgatga gcaccaccag gacctgaccc tgcttaaggc actcgtccgg cagcagcttc
4621 cggagaagta caaggaaatc ttctttgacc agtcaaagaa tggatacgcc ggctacatcg
4681 acggaggtgc ctcccaagag gaattttata agtttatcaa acctatcctt gagaagatgg
4741 acggcaccga agagctcctc gtgaaactga atcgggagga tctgctgcgg aagcagcgca
4801 ctttcgacaa tgggagcatt ccccaccaga tccatcttgg ggagcttcac gccatccttc
4861 ggcgccaaga ggacttctac ccctttctta aggacaacag ggagaagatt gagaaaattc
4921 tcactttccg catcccctac tacgtgggac ccctcgccag aggaaatagc cggtttgctt
4981 ggatgaccag aaagtcagaa gaaactatca ctccctggaa cttcgaagag gtggtggaca
5041 agggagccag cgctcagtca ttcatcgaac ggatgactaa cttcgataag aacctcccca
5101 atgagaaggt cctgccgaaa cattccctgc tctacgagta ctttaccgtg tacaacgagc
5161 tgaccaaggt gaaatatgtc accgaaggga tgaggaagcc cgcattcctg tcaggcgaac
5221 aaaagaaggc aattgtggac cttctgttca agaccaatag aaaggtgacc gtgaagcagc
5281 tgaaggagga ctatttcaag aaaattgaat gcttcgactc tgtggagatt agcggggtcg
5341 aagatcggtt caacgcaagc ctgggtacct accatgatct gcttaagatc atcaaggaca
5401 aggattttct ggacaatgag gagaacgagg acatccttga ggacattgtc ctgactctca
5461 ctctgttcga ggaccgggaa atgatcgagg agaggcttaa gacctacgcc catctgttcg
5521 acgataaagt gatgaagcaa cttaaacgga gaagatatac cggatgggga cgccttagcc
5581 gcaaactcat caacggaatc cgggacaaac agagcggaaa gaccattctt gatttcctta
5641 agagcgacgg attcgctaat cgcaacttca tgcaacttat ccatgatgat tccctgacct
5701 ttaaggagga catccagaag gcccaagtgt ctggacaagg tgactcactg cacgagcata
5761 tcgcaaatct ggctggttca cccgctatta agaagggtat tctccagacc gtgaaagtcg
5821 tggacgagct ggtcaaggtg atgggtcgcc ataaaccaga gaacattgtc atcgagatgg
5881 ccagggaaaa ccagactacc cagaagggac agaagaacag cagggagcgg atgaaaagaa
5941 ttgaggaagg gattaaggag ctcgggtcac agatccttaa agagcacccg gtggaaaaca
6001 cccagcttca gaatgagaag ctctatctgt actaccttca aaatggacgc gatatgtatg
6061 tggaccaaga gcttgatatc aacaggctct cagactacga cgtggacgcc atcgtccctc
6121 agagcttcct caaagacgac tcaattgaca ataaggtgct gactcgctca gacaagaacc
6181 ggggaaagtc agataacgtg ccctcagagg aagtcgtgaa aaagatgaag aactattggc
6241 gccagcttct gaacgcaaag ctgatcactc agcggaagtt cgacaatctc actaaggctg
6301 agaggggcgg actgagcgaa ctggacaaag caggattcat taaacggcaa cttgtggaga
6361 ctcggcagat tactaaacat gtcgcccaaa tccttgactc acgcatgaat accaagtacg
6421 acgaaaacga caaacttatc cgcgaggtga aggtgattac cctgaagtcc aagctggtca
6481 gcgatttcag aaaggacttt caattctaca aagtgcggga gatcaataac tatcatcatg
6541 ctcatgacgc atatctgaat gccgtggtgg gaaccgccct gatcaagaag tacccaaagc
6601 tggaaagcga gttcgtgtac ggagactaca aggtctacga cgtgcgcaag atgattgcca
6661 aatctgagca ggagatcgga aaggccaccg caaagtactt cttctacagc aacatcatga
6721 atttcttcaa gaccgaaatc acccttgcaa acggtgagat ccggaagagg ccgctcatcg
6781 agactaatgg ggagactggc gaaatcgtgt gggacaaggg cagagatttc gctaccgtgc
6841 gcaaagtgct ttctatgcct caagtgaaca tcgtgaagaa aaccgaggtg caaaccggag
6901 gcttttctaa ggaatcaatc ctccccaagc gcaactccga caagctcatt gcaaggaaga
6961 aggattggga ccctaagaag tacggcggat tcgattcacc aactgtggct tattctgtcc
7021 tggtcgtggc taaggtggaa aaaggaaagt ctaagaagct caagagcgtg aaggaactgc
7081 tgggtatcac cattatggag cgcagctcct tcgagaagaa cccaattgac tttctcgaag
7141 ccaaaggtta caaggaagtc aagaaggacc ttatcatcaa gctcccaaag tatagcctgt
7201 tcgaactgga gaatgggcgg aagcggatgc tcgcctccgc tggcgaactt cagaagggta
7261 atgagctggc tctcccctcc aagtacgtga atttcctcta ccttgcaagc cattacgaga
7321 agctgaaggg gagccccgag gacaacgagc aaaagcaact gtttgtggag cagcataagc
7381 attatctgga cgagatcatt gagcagattt ccgagttttc taaacgcgtc attctcgctg
7441 atgccaacct cgataaagtc cttagcgcat acaataagca cagagacaaa ccaattcggg
7501 agcaggctga gaatatcatc cacctgttca ccctcaccaa tcttggtgcc cctgccgcat
7561 tcaagtactt cgacaccacc atcgaccgga aacgctatac ctccaccaaa gaagtgctgg
7621 acgccaccct catccaccag agcatcaccg gactttacga aactcggatt gacctctcac
7681 agctcggagg ggatgaggga gctcccaaga aaaagcgcaa ggtaggtagt tccgccggaa
7741 aggccggtga aggtgaaatc cctgcccctc ttgctggtac cgtttctaag atactggtaa
7801 aagaaggtga cactgttaaa gctggtcaaa cagttctggt gctggaggct atgaaaatgg
7861 agacagaaat taacgctcct actgacggaa aagttgaaaa ggtgttagtt aaggaaagag
7921 atgctgttca aggtggtcaa ggtctaatca agatcggcgt ttaagatccc gcccctctcc
7981 ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
8041 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
8101 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
8161 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
8221 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
8281 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
8341 ttggatagtt gtggaaagag tcaaatggct cacctcaagc gtattcaaca aggggctgaa
8401 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt
8461 tacatgtgtt tagtcgaggt taaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt
8521 ttcctttgaa aaacacgatg ataatatggc ccagtccaag cacggcctga ccaaggagat
8581 gaccatgaag taccgcatgg agggctgcgt ggacggccac aagttcgtga tcaccggcga
8641 gggcatcggc taccccttca agggcaagca ggccatcaac ctgtgcgtgg tggagggcgg
8701 ccccttgccc ttcgccgagg acatcttgtc cgccgccttc atgtacggca accgcgtgtt
8761 caccgagtac ccccaggaca tcgtcgacta cttcaagaac tcctgccccg ccggctacac
8821 ctgggaccgc tccttcctgt tcgaggacgg cgccgtgtgc atctgcaacg ccgacatcac
8881 cgtgagcgtg gaggagaact gcatgtacca cgagtccaag ttctacggcg tgaacttccc
8941 cgccgacggc cccgtgatga agaagatgac cgacaactgg gagccctcct gcgagaagat
9001 catccccgtg cccaagcagg gcatcttgaa gggcgacgtg agcatgtacc tgctgctgaa
9061 ggacggtggc cgcttgcgct gccagttcga caccgtgtac aaggccaagt ccgtgccccg
9121 caagatgccc gactggcact tcatccagca caagctgacc cgcgaggacc gcagcgacgc
9181 caagaaccag aagtggcacc tgaccgagca cgccatcgcc tccggctccg ccttgccctg
9241 aacgcgtctg gaacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
9301 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
9361 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
9421 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
9481 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
9541 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
9601 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
9661 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
9721 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
9781 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
9841 cgcctggaat taattctgca gtcgagacct agaaaaacat ggagcaatca caagtagcaa
9901 tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg
9961 ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct
10021 tagccacttt ttaaaagaaa agaggggact ggaagggcta attcactccc aacgaagaca
10081 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta
10141 cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc
10201 agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc
10261 tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag
10321 ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg
10381 atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg
10441 cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta
10501 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
10561 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
10621 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
10681 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
10741 cagagagtga gaggccttga cattgctagc gtttaccgtc gacctctagc tagagcttgg
10801 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
10861 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
10921 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
10981 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
11041 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
11101 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
11161 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
11221 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
11281 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
11341 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
11401 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
11461 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
11521 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
11581 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
11641 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
11701 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
11761 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
11821 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
11881 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
11941 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
12001 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
12061 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
12121 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
12181 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
12241 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
12301 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
12361 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
12421 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
12481 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
12541 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
12601 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
12661 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
12721 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
12781 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
12841 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
12901 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
12961 ctgacgtcga cggatcggga gatcaacttg tttattgcag cttataatgg ttacaaataa
13021 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
13081 ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac
13141 caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt
13201 catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt
13261 taaatatgta ctacaaactt agtagt
//
