pMSCVhyg

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pMSCV-Hygro载体基本信息

出品公司: Clontech
载体名称: pMSCVhyg, pMSCV-hygro, pMSCV hyg
质粒类型: 哺乳动物细胞逆病毒基因表达载体
高拷贝/低拷贝: 低拷贝
启动子: LTR
克隆方法: 多克隆位点,限制性内切酶
载体大小: 6945bp
5' 测序引物及序列: MSCV-F: CCCTTGAACCTCCTCGTTCGACC
3' 测序引物及序列: pMSCV-R: GAGACGTGCTACTTCCATTTGTC
载体标签: --
载体抗性: 氨苄
筛选标记: 潮霉素(Hygromycin)
备注: 逆病毒表达载体,优化后,有利于在造血干细胞中和癌细胞中进行基因表达。
产品目录号: 634401
稳定性: 稳表达
组成型: 组成型
病毒/非病毒: 逆病毒

pMSCV-Hygro载体质粒图谱和多克隆位点信息

pMSCVhyg载体图谱



pMSCVhyg多克隆位点

RNAi-Ready pSIREN-RetroQ载体特征

pMSCV-Hygro载体简介

pMSCV-Hygro载体序列

LOCUS       pMSCVhyg   6945 bp    DNA   circular             4-DEC-2009
COMMENT     Created by Clontech Laboratories Inc. 
COMMENT     Cat. No. 631461,634401
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     LTR             3368..3851
                     /label=3'\PCMV\LTR
     LTR             1..515
                     /label=5'\MMSV\LTR
     CDS             complement(5154..6011)
                     /label=Amp(R)
     rep_origin      4391..4391
                     /label=pUC\origin
     CDS             1955..2980
                     /label=Hyg(R)
     promoter        1429..1937
                     /label=Phosphoglycerate\Kinase\Promoter
     misc_signal     516..1404
                     /label=Extended\packaging\signal
BASE COUNT     1578 a      1900 c      1857 g      1610 t 
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat 
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca 
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga 
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt 
      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc 
      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc 
      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata 
      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca 
      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga 
      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg 
      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt 
      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa 
      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga 
      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag 
      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa 
      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg 
      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct 
     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga 
     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag 
     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc 
     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc 
     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg 
     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag 
     1381 ccctcactcc ttctctaggc gccggaatta gatctctcga ggttaacgaa ttctaccggg 
     1441 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca 
     1501 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc 
     1561 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga 
     1621 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc 
     1681 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg 
     1741 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg 
     1801 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg 
     1861 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct 
     1921 catctccggg cctttcgacc tgcatcccgc caccatgaaa aagcctgaac tcaccgcgac 
     1981 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc 
     2041 ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg 
     2101 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc 
     2161 ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta 
     2221 ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc 
     2281 cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca 
     2341 gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga 
     2401 tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac 
     2461 cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc 
     2521 cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg 
     2581 ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt 
     2641 cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt 
     2701 cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat 
     2761 tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc 
     2821 gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat 
     2881 cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg 
     2941 aaaccgacgc cccagcactc gtccgagggc aaaggaatag agtagatgcc gaccgaacaa 
     3001 gagctgattt cgagaacgcc tcagccagca actcgcgcga gcctagcaag gcaaatgcga 
     3061 gagaacggcc ttacgcttgg tggcacagtt ctcgtccaca gttcgctaag ctcgctcggc 
     3121 tgggtcgcgg gagggccggt cgcagtgatt caggcccttc tggattgtgt tggtccccag 
     3181 ggcacgattg tcatgcccac gcactcgggt gatctgactg atcccgcaga ttggagatcg 
     3241 ccgcccgtgc ctgccgattg ggtgcagatc cgtcgacctg cagccaagct tatcgataaa 
     3301 ataaaagatt ttatttagtc tccagaaaaa ggggggaatg aaagacccca cctgtaggtt 
     3361 tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaatacata actgagaata 
     3421 gagaagttca gatcaaggtt aggaacagag agacagcaga atatgggcca aacaggatat 
     3481 ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggtccc cagatgcggt 
     3541 cccgccctca gcagtttcta gagaaccatc agatgtttcc agggtgcccc aaggacctga 
     3601 aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg 
     3661 cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggcgc gccagtcctc 
     3721 cgatagactg cgtcgcccgg gtacccgtgt atccaataaa ccctcttgca gttgcatccg 
     3781 acttgtggtc tcgctgttcc ttgggagggt ctcctctgag tgattgacta cccgtcagcg 
     3841 ggggtctttc atgggtaaca gtttcttgaa gttggagaac aacattctga gggtaggagt 
     3901 cgaatattaa gtaatcctga ctcaattagc cactgttttg aatccacata ctccaatact 
     3961 cctgaaatag ttcattatgg acagcgcaga aagagctggg gagaattgtg aaattgttat 
     4021 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc 
     4081 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga 
     4141 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt 
     4201 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 
     4261 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 
     4321 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 
     4381 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 
     4441 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 
     4501 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 
     4561 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 
     4621 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 
     4681 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 
     4741 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 
     4801 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg 
     4861 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 
     4921 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 
     4981 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 
     5041 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 
     5101 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc 
     5161 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 
     5221 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 
     5281 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 
     5341 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 
     5401 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc 
     5461 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt 
     5521 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc 
     5581 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg 
     5641 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt 
     5701 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg 
     5761 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 
     5821 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 
     5881 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 
     5941 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 
     6001 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 
     6061 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 
     6121 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat 
     6181 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac 
     6241 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc 
     6301 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat 
     6361 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga 
     6421 tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg 
     6481 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct 
     6541 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg 
     6601 gcgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg 
     6661 ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc 
     6721 catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg 
     6781 ccacgatgcg tccggcgtag aggcgattag tccaatttgt taaagacagg atatcagtgg 
     6841 tccaggctct agttttgact caacaatatc accagctgaa gcctatagag tacgagccat 
     6901 agataaaata aaagatttta tttagtctcc agaaaaaggg gggaa 
//

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pMSCV-Hygro载体应用举例

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