pDEST32

价格:1500元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Invitrogen
载体名称: pDEST32
质粒类型: Gateway载体;酵母双杂交载体
高拷贝/低拷贝: 高拷贝
启动子: ADH1
克隆方法: Gateway
载体大小: 12266bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: Ampicillin
筛选标记: Gentamycin
备注: 含Beta-Gal 报告基因
产品目录号: PQ10001-01
稳定性: --
组成型: --
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pDEST32载体图谱
pDEST32多克隆位点

pDEST32载体特征

载体简介

采用 Gateway® 技术的 ProQuest™ 双杂交系统 (ProQuest™ Two-Hybrid System with Gateway® Technology) 是一种基于酵母的体内系统,用于鉴定两种蛋白之间的相互作用。 这种相互作用会重构功能性转录因子,该转录因子激活整合在染色体上的报告基因,而转录激活与否可通过选择性培养基上的细胞生长情况来进行监测。
ProQuest™ 系统 (ProQuest™ System) 具有如下特点:
• 采用 Gateway® 技术,能够快速简便地生成“诱饵”和“猎物”构建体,有利于下游应用
• 低拷贝 DBD(DNA 结合结构域)和 AD(活化结构域)载体 (ARS/CEN),可控制过量表达,提高重复性
• 三种不同报告基因(HIS3、URA3 和 lacZ),具有独立的启动子区域,可快速筛除假阳性。 URA3 报告基因可用于阳性选择和阴性选择,从而可以使用高级双杂交技术,例如反向双杂交。

• 一组由强到弱、基于 Gateway® 的相互作用对照组,用于评估实验结果以及实验组相互作用的强度。

载体序列

LOCUS       pDEST32                12288 bp    DNA     circular SYN  2-SEP-2003
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTDATE|38836800|
COMMENT     VNTDBDATE|312633458|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pDEST32|
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..12288
                     /invitrogen="666"
                     /vntifkey="98"
     misc_recomb     6792..6916
                     /invitrogen="2690000"
                     /vntifkey="86"
                     /label=attR1
     misc_recomb     complement(8513..8637)
                     /invitrogen="2700000"
                     /vntifkey="86"
                     /label=attR2
     CDS             8167..8472
                     /invitrogen="1060000"
                     /vntifkey="4"
                     /label=ccdB
     CDS             7166..7825
                     /invitrogen="1080000"
                     /vntifkey="4"
                     /label=Cm(R)
     rep_origin      9359..9814
                     /invitrogen="2520000"
                     /vntifkey="33"
                     /label=f1\origin
     CDS             6336..6791
                     /invitrogen="1120000"
                     /vntifkey="4"
                     /label=Gal4\DBD
     CDS             1450..1983
                     /invitrogen="1150000"
                     /vntifkey="4"
                     /label=Gm(R)
     CDS             10520..11626
                     /invitrogen="1250000"
                     /vntifkey="4"
                     /label=LEU2
     terminator      complement(3399..3647)
                     /invitrogen="2560000"
                     /vntifkey="43"
                     /label=CYC1\transcription\terminator
     promoter        1234..1261
                     /invitrogen="2250000"
                     /vntifkey="29"
                     /label=Pc\promoter
     rep_origin      2313..2986
                     /invitrogen="2540000"
                     /vntifkey="33"
                     /label=pUC\origin
     terminator      8837..9168
                     /invitrogen="2565000"
                     /vntifkey="43"
                     /label=ADH1\transcription\terminator
     promoter        4857..6308
                     /invitrogen="2221000"
                     /vntifkey="29"
                     /label=ADH1\promoter
     CDS             complement(3940..4389)
                     /invitrogen="1335000"
                     /vntifkey="4"
                     /label=CYH2
     promoter        complement(4390..4705)
                     /invitrogen="2222000"
                     /vntifkey="29"
                     /label=CYH2\promoter
     3'UTR           complement(3755..3939)
                     /invitrogen="123000098"
                     /vntifkey="50"
                     /label=CYH2\3'\UTR
     centromere      68..443
                     /invitrogen="123000099"
                     /vntifkey="5"
                     /label=ARS4/Cen6
BASE COUNT     3472 a      2635 c      2722 g      3458 t         1 others
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 
       61 cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa tgatggaata 
      121 atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat tttagaaagt 
      181 aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa 
      241 atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc agattaaata 
      301 gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg tgtgtggtct 
      361 tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa gagcaagata 
      421 aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa aacaaaaact 
      481 attttttctt taatttcttt ttttactttc tatttttaat ttatatattt atattaaaaa 
      541 atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg cacttttcgg 
      601 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 
      661 ctcatgagac aataaccctg ataaatgctt caataatctg cagtgcgcag ggcccgtgtc 
      721 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact 
      781 gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc 
      841 ttgctggagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc 
      901 tcggtagcca accactagaa ctatagctag agtcctgggc gaacaaacga tgctcgcctt 
      961 ccagaaaacc gaggatgcga accacttcat ccggggtcag caccaccggc aagcgccgcg 
     1021 acggccgagg tcttccgatc tcctgaagcc agggcagatc cgtgcacagc accttgccgt 
     1081 agaagaacag caaggccgcc aatgcctgac gatgcgtgga gaccgaaacc ttgcgctcgt 
     1141 tcgccagcca ggacagaaat gcctcgactt cgctgctgcc caaggttgcc gggtgacgca 
     1201 caccgtggaa acggatgaag gcacgaaccc agttgacata agcctgttcg gttcgtaaac 
     1261 tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg 
     1321 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt gtacagtcta 
     1381 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga 
     1441 gcagcaacga tgttacgcag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca 
     1501 aagttaggtg gctcaagtat gggcatcatt cgcacatgta ggctcggccc tgaccaagtc 
     1561 aaatccatgc gggctgctct tgatcttttc ggtcgtgagt tcggagacgt agccacctac 
     1621 tcccaacatc agccggactc cgattacctc gggaacttgc tccgtagtaa gacattcatc 
     1681 gcgcttgctg ccttcgacca agaagcggtt gttggcgctc tcgcggctta cgttctgccc 
     1741 aggtttgagc agccgcgtag tgagatctat atctatgatc tcgcagtctc cggcgagcac 
     1801 cggaggcagg gcattgccac cgcgctcatc aatctcctca agcatgaggc caacgcgctt 
     1861 ggtgcttatg tgatctacgt gcaagcagat tacggtgacg atcccgcagt ggctctctat 
     1921 acaaagttgg gcatacggga agaagtgatg cactttgata tcgacccaag taccgccacc 
     1981 taacaattcg ttcaagccga gatcggcttc ccggcctaat aggttgtatt gatgttggac 
     2041 gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt 
     2101 tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga 
     2161 ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt 
     2221 tgtaacactg gcagagcatt acgctgactt gacgggacgg cgncatgacc aaaatccctt 
     2281 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 
     2341 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 
     2401 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 
     2461 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca 
     2521 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 
     2581 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 
     2641 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 
     2701 acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga 
     2761 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 
     2821 ttccaggggg gaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 
     2881 agcgtcgatt tttgtgatgc tcgtcagggg ggccgagcct atggaaaaac gccagcaacg 
     2941 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt 
     3001 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc 
     3061 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac 
     3121 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc 
     3181 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttacctc actcattagg 
     3241 caccccaggc tttacacttt atgcttccgg ctcctatgtt gtgtggaatt gtgagcggat 
     3301 aacaatttca cacaggaaac agctatgacc atgattacgc caagctcgga attaaccctc 
     3361 actaaaggga acaaaagctg gtaccgatcc cgagctttgc aaattaaagc cttcgagcgt 
     3421 cccaaaacct tctcaagcaa ggttttcagt ataatgttac atgcgtacac gcgtctgtac 
     3481 agaaaaaaaa gaaaaatttg aaatataaat aacgttctta atactaacat aactataaaa 
     3541 aaataaatag ggacctagac ttcaggttgt ctaactcctt ccttttcggt tagagcggat 
     3601 gtggggggag ggcgtgaatg taagcgtgac ataactaatt acatgatatc gacaaaggaa 
     3661 aaggggcctg tttactcaca ggcttttttc aagtaggtaa ttaagtcgtt tctgtctttt 
     3721 tccttcttca acccaccaaa ggccatcttg gtactttttt tttttttttt tttttttttt 
     3781 tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt 
     3841 ttttttttca tagaaataat acagaagtag atgttgaatt agattaaact gaagatatat 
     3901 aatttattgg aaaatacata gagctttttg ttgatgcgct taagcgatca attcaacaac 
     3961 accaccagca gctctgattt tttcttcagc caacttggag acgaatctag ctttgacgat 
     4021 aactggaaca tttggaattc tacccttacc caagatctta ccgtaaccgg ctgccaaagt 
     4081 gtcaataact ggagcagttt ccttagaagc agatttcaag tattggtctc tcttgtcttc 
     4141 tgggatcaat gtccacaatt tgtccaagtt caagactggc ttccagaaat gagcttgttg 
     4201 cttgtggaag tatctcatac caaccttacc gaaataacct ggatggtatt tatccatgtt 
     4261 aattctgtgg tgatgttgac caccggccat acctctacca ccggggtgct ttctgtgctt 
     4321 accgatacga cctttaccgg ctgagacgtg acctctgtgc tttctagtct tagtgaatct 
     4381 ggaaggcatt cttgattagt tggatgattg ttctgggatt taatgcaaaa atcacttaag 
     4441 aaggaaaatc aacggagaaa gcaaacgcca tcttaaatat acgggataca gatgaaaggg 
     4501 tttgaaccta tctggaaaat agcattaaac aagcgaaaaa ctgcgaggaa aattgtttgc 
     4561 gtctctgcgg gctattcacg cgccagagga aaataggaaa aataacaggg cattagaaaa 
     4621 ataattttga ttttggtaat gtgtgggtcc tggtgtacag atgttacatt ggttacagta 
     4681 ctcttgtttt tgctgtgttt ttcgatgaat ctccaaaatg gttgttagca catggaagag 
     4741 tcaccgatgc taagttatct ctatgtaagc tacgtggcgt gacttttgat gaagccgcac 
     4801 aagagataca ggattggcaa ctgcaaatag aatctgggga tcccccctcg agatccggga 
     4861 tcgaagaaat gatggtaaat gaaataggaa atcaaggagc atgaaggcaa aagacaaata 
     4921 taagggtcga acgaaaaata aagtgaaaag tgttgatatg atgtatttgg ctttgcggcg 
     4981 ccgaaaaaac gagtttacgc aattgcacaa tcatgctgac tctgtggcgg acccgcgctc 
     5041 ttgccggccc ggcgataacg ctgggcgtga ggctgtgccc ggcggagttt tttgcgcctg 
     5101 cattttccaa ggtttaccct gcgctaaggg gcgagattgg agaagcaata agaatgccgg 
     5161 ttggggttgc gatgatgacg accacgacaa ctggtgtcat tatttaagtt gccgaaagaa 
     5221 cctgagtgca tttgcaacat gagtatacta gaagaatgag ccaagacttg cgagacgcga 
     5281 gtttgccggt ggtgcgaaca atagagcgac catgaccttg aaggtgagac gcgcataacc 
     5341 gctagagtac tttgaagagg aaacagcaat agggttgcta ccagtataaa tagacaggta 
     5401 catacaacac tggaaatggt tgtctgtttg agtacgcttt caattcattt gggtgtgcac 
     5461 tttattatgt tacaatatgg aagggaactt tacacttctc ctatgcacat atattaatta 
     5521 aagtccaatg ctagtagaga aggggggtaa cacccctccg cgctcttttc cgattttttt 
     5581 ctaaaccgtg gaatatttcg gatatccttt tgttgtttcc gggtgtacaa tatggacttc 
     5641 ctcttttctg gcaaccaaac ccatacatcg ggattcctat aataccttcg ttggtctccc 
     5701 taacatgtag gtggcggagg ggagatatac aatagaacag ataccagaca agacataatg 
     5761 ggctaaacaa gactacacca attacactgc ctcattgatg gtggtacata acgaactaat 
     5821 actgtagccc tagacttgat agccatcatc atatcgaagt ttcactaccc tttttccatt 
     5881 tgccatctat tgaagtaata ataggcgcat gcaacttctt ttcttttttt ttcttttctc 
     5941 tctcccccgt tgttgtctca ccatatccgc aatgacaaaa aaaatgatgg aagacactaa 
     6001 aggaaaaaat taacgacaaa gacagcacca acagatgtcg ttgttccaga gctgatgagg 
     6061 ggtatcttcg aacacacgaa actttttcct tccttcattc acgcacacta ctctctaatg 
     6121 agcaacggta tacggccttc cttccagtta cttgaatttg aaataaaaaa agtttgccgc 
     6181 tttgctatca agtataaata gacctgcaat tattaatctt ttgtttcctc gtcattgttc 
     6241 tcgttccctt tcttccttgt ttctttttct gcacaatatt tcaagctata ccaagcatac 
     6301 aatcaactcc aagcttgaag caagcctcct gaaagatgaa gctactgtct tctatcgaac 
     6361 aagcatgcga tatttgccga cttaaaaagc tcaagtgctc caaagaaaaa ccgaagtgcg 
     6421 ccaagtgtct gaagaacaac tgggagtgtc gctactctcc caaaaccaaa aggtctccgc 
     6481 tgactagggc acatctgaca gaagtggaat caaggctaga aagactggaa cagctatttc 
     6541 tactgatttt tcctcgagaa gaccttgaca tgattttgaa aatggattct ttacaggata 
     6601 taaaagcatt gttaacagga ttatttgtac aagataatgt gaataaagat gccgtcacag 
     6661 atagattggc ttcagtggag actgatatgc ctctaacatt gagacagcat agaataagtg 
     6721 cgacatcatc atcggaagag agtagtaaca aaggtcaaag acagttgact gtatcgtcga 
     6781 ggtcgaatca aacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata 
     6841 tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac 
     6901 atatccagtc actatggcgg ccgctaagtt ggcagcatca cccgacgcac tttgcgccga 
     6961 ataaatacct gtgacggaag atcacttcgc agaataaata aatcctggtg tccctgttga 
     7021 taccgggaag ccctgggcca acttttggcg aaaatgagac gttgatcggc acgtaagagg 
     7081 ttccaacttt caccataatg aaataagatc actaccgggc gtattttttg agttatcgag 
     7141 attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga 
     7201 tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac 
     7261 ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa 
     7321 gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga 
     7381 attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta 
     7441 caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga 
     7501 tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc 
     7561 ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag 
     7621 tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac 
     7681 catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca 
     7741 tcatgccgtc tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg 
     7801 cgatgagtgg cagggcgggg cgtaatctag aggatccggc ttactaaaag ccagataaca 
     7861 gtatgcgtat ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg 
     7921 aagtatgtca aaaagaggtg tgctatgaag cagcgtatta cagtgacagt tgacagcgac 
     7981 agctatcagt tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca 
     8041 tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga 
     8101 tggctgaggt cgcccggttt attgaaatga acggctcttt tgctgacgag aacagggact 
     8161 ggtgaaatgc agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg 
     8221 gatgtacaga gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt 
     8281 gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat 
     8341 gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa 
     8401 gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc 
     8461 tggggaatat aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga 
     8521 ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa 
     8581 tatattgata tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggtttg 
     8641 atggccgcta agtaagtaag acgtcgagct ctaagtaagt aacggccgcc accgcggtgg 
     8701 agctttggac ttcttcgcca gaggtttggt caagtctcca atcaaggttg tcggcttgtc 
     8761 taccttgcca gaaatttacg aaaagatgga aaagggtcaa atcgttggta gatacgttgt 
     8821 tgacacttct aaataagcga atttcttatg atttatgatt tttattatta aataagttat 
     8881 aaaaaaaata agtgtataca aattttaaag tgactcttag gttttaaaac gaaaattctt 
     8941 gttcttgagt aactctttcc tgtaggtcag gttgctttct caggtatagc atgaggtcgc 
     9001 tcttattgac cacacctcta ccggcatgcc gagcaaatgc ctgcaaatcg ctccccattt 
     9061 cacccaattg tagatatgct aactccagca atgagttgat gaatctcggt gtgtatttta 
     9121 tgtcctcaga ggacaatacc tgttgtaatc gttcttccac acggatccca attcgcccta 
     9181 tagtgagtcg tattacaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc 
     9241 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag 
     9301 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggac 
     9361 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct 
     9421 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg 
     9481 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt 
     9541 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca 
     9601 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga 
     9661 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa 
     9721 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac 
     9781 gcgaatttta acaaaatatt aacgtttaca atttcctgat gcggtatttt ctccttacgc 
     9841 atctgtgcgg tatttcacac cgcatatcga ccggtcgagg agaacttcta gtatatccac 
     9901 atacctaata ttattgcctt attaaaaatg gaatcggaac aattacatca aaatccacat 
     9961 tctcttcaaa atcaattgtc ctgtacttcc ttgttcatgt gtgttcaaaa acgttatatt 
    10021 tataggataa ttatactcta tttctcaaca agtaattggt tgtttggccg agcggtctaa 
    10081 ggcgcctgat tcaagaaata tcttgaccgc agttaactgt gggaatactc aggtatcgta 
    10141 agatgcaaga gttcgaatct cttagcaacc attatttttt tcctcaacat aacgagaaca 
    10201 cacaggggcg ctatcgcaca gaatcaaatt cgatgactgg aaattttttg ttaatttcag 
    10261 aggtcgcctg acgcatatac ctttttcaac tgaaaaattg ggagaaaaag gaaaggtgag 
    10321 aggccggaac cggcttttca tatagaatag agaagcgttc atgactaaat gcttgcatca 
    10381 caatacttga agttgacaat attatttaag gacctattgt tttttccaat aggtggttag 
    10441 caatcgtctt actttctaac ttttcttacc ttttacattt cagcaatata tatatatatt 
    10501 tcaaggatat accattctaa tgtctgcccc tatgtctgcc cctaagaaga tcgtcgtttt 
    10561 gccaggtgac cacgttggtc aagaaatcac agccgaagcc attaaggttc ttaaagctat 
    10621 ttctgatgtt cgttccaatg tcaagttcga tttcgaaaat catttaattg gtggtgctgc 
    10681 tatcgatgct acaggtgtcc cacttccaga tgaggcgctg gaagcctcca agaaggttga 
    10741 tgccgttttg ttaggtgctg tgggtggtcc taaatggggt accggtagtg ttagacctga 
    10801 acaaggttta ctaaaaatcc gtaaagaact tcaattgtac gccaacttaa gaccatgtaa 
    10861 ctttgcatcc gactctcttt tagacttatc tccaatcaag ccacaatttg ctaaaggtac 
    10921 tgacttcgtt gttgtcagag aattagtggg aggtatttac tttggtaaga gaaaggaaga 
    10981 cgatggtgat ggtgtcgctt gggatagtga acaatacacc gttccagaag tgcaaagaat 
    11041 cacaagaatg gccgctttca tggccctaca acatgagcca ccattgccta tttggtcctt 
    11101 ggataaagct aatgttttgg cctcttcaag attatggaga aaaactgtgg aggaaaccat 
    11161 caagaacgaa ttccctacat tgaaggttca acatcaattg attgattctg ccgccatgat 
    11221 cctagttaag aacccaaccc acctaaatgg tattataatc accagcaaca tgtttggtga 
    11281 tatcatctcc gatgaagcct ccgttatccc aggttccttg ggtttgttgc catctgcgtc 
    11341 cttggcctct ttgccagaca agaacaccgc atttggtttg tacgaaccat gccacggttc 
    11401 tgctccagat ttgccaaaga ataaggttga ccctatcgcc actatcttgt ctgctgcaat 
    11461 gatgttgaaa ttgtcattga acttgcctga agaaggtaag gccattgaag atgcagttaa 
    11521 aaaggttttg gatgcaggta tcagaactgg tgatttaggt ggttccaaca gtaccaccga 
    11581 agtcggtgat gctgtcgccg aagaagttaa gaaaatcctt gcttaaaaag attctctttt 
    11641 tttatgatat ttgtacataa actttataaa tgaaattcat aatagaaacg acacgaaatt 
    11701 acaaaatgga atatgttcat agggtagacg aaactatata cgcaatctac atacatttat 
    11761 caagaaggag aaaaaggagg atagtaaagg aatacaggta agcaaattga tactaatggc 
    11821 tcaacgtgat aaggaaaaag aattgcactt taacattaat attgacaagg aggagggcac 
    11881 cacacaaaaa gttaggtgta acagaaaatc atgaaactac gattcctaat ttgatattgg 
    11941 aggattttct ctaaaaaaaa aaaaatacaa caaataaaaa acactcaatg acctgaccat 
    12001 ttgatggagt ttaagtcaat accttcttga accatttccc ataatggtga aagttccctc 
    12061 aagaatttta ctctgtcaga aacggcctta cgacgtagtc gatatggtgc actctcagta 
    12121 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg 
    12181 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg 
    12241 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcga 
//

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