pET-15b

价格:800元

联系方式:I47-825O-882O

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pet15b载体基本信息

出品公司: EMD Biosciences(Novagen)
别名: pET15b, pet 15b
质粒类型: 大肠杆菌蛋白表达
表达水平:
克隆方法: 多克隆位点,限制性内切酶
载体大小: 5708bp
5' 测序引物: T7
5' 测序引物序列:   5'-TAATACGACTCACTATAGGG-3'
载体标签: N-His, N-thrombin
载体抗性: Ampicillin
备注:
Hosts: E.coli. Related vectors: pBR322.
产品目录号: 69661-3
稳定性: 瞬时表达 Transient
组成型: 组成型 Constitutive
病毒/非病毒: 非病毒

pet15b载体质粒图谱和多克隆位点信息

pET-15b载体图谱



pET-15b多克隆位点

pET-15b载体特征

Feature Name Start End  
T7_terminator 316 188
T7_Terminal_primer 256 274
T7_transl_en_RBS 416 400
lacO 461 434
T7_promoter 479 461
pBRrevBam_primer 582 563
lacI 857 1948
tet (576 - 1190) 2007 2621
ROP 3260 3451
pGEX_3_primer 3467 3445
Ampicillin 5500 4640
AmpR_promoter 5570 5542
ORF Start End  
ORF frame 1 616 1116
ORF frame 2 989 1948
ORF frame 1 2426 1731
ORF frame 3 2004 2621
ORF frame 2 5500 4640
Enzyme Name Cut
ClaI 24
HindIII 29
BamHI 319
XhoI 324
NdeI 331
NcoI 389
XbaI 428
BglII 494
BclI 1230
ApaI 1427
HpaI 1722
EagI 2284
NruI 2319
MscI 2791
PstI 4958
AatII 5635
EcoRI 5706

pet15b载体简介

pET Vectors 选择指南

pet15b载体序列

LOCUS       pET-15b    5708 bp     DNA  circular   SYN
DEFINITION  pET-15b
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTOAUTHORAD1|Novagen Inc.|
FEATURES             Location/Qualifiers
     source          1..5708
                     /organism="pET-15b"
                     /mol_type="other DNA"
     terminator      complement(188..316)
                     /label="T7_terminator"
     misc_feature    256..274
                     /label="T7_Terminal_primer"
     misc_feature    complement(400..416)
                     /label="T7_transl_en_RBS"
     misc_feature    complement(434..461)
                     /label="lacO"
     promoter        complement(461..479)
                     /label="T7_promoter"
     misc_feature    complement(563..582)
                     /label="pBRrevBam_primer"
     CDS             616..1116
                     /label="ORF frame 1"
                     /translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF
                     LMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECETSNVIRCRRVCRCLLSD
                     RFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTVVADWRCHLQSGPARAV
                     ANCRGD*"
     misc_feature    857..1948
                     /label="lacI"
                     /translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF
                     LMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECETSNVIRCRRVCRCLLSD
                     RFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTVVADWRCHLQSGPARAV
                     ANCRGD*"
     CDS             989..1948
                     /label="ORF frame 2"
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                     SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
     CDS             complement(1731..2426)
                     /label="ORF frame 1"
                     /translation="MVVIYLPGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQ
                     RVGRHAGDNGLLLAETFGGGTSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPER
                     CRHLSYELHDKEDSHKCGDDSHAPRPPEGADWVEGSQGHRSRSRCLMSELTYINCVAL
                     TARFPVGKPVVPAALMNRPTRGERRFAYWAPGWFFFSPVRRATADCPSPPGPERVAAS
                     GPRWFAPAGENPV*"
     CDS             2004..2621
                     /label="ORF frame 3"
                     /translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
                     FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
                     LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG
                     PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
     gene            2007..2621
                     /label="tet (576 - 1190)"
                     /gene="tet (576 - 1190)"
                     /translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
                     FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
                     LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG
                     PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
     misc_feature    3260..3451
                     /label="ROP"
                     /translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
                     FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
                     LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG
                     PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
     misc_feature    complement(3445..3467)
                     /label="pGEX_3_primer"
                     /translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
                     FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
                     LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG
                     PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
     gene            complement(4640..5500)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
                     FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
                     LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG
                     PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
     CDS             complement(4640..5500)
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(5542..5570)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
   61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
  121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
  181 gcgggatatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag
  241 gccccaaggg gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt
  301 cgggctttgt tagcagccgg atcctcgagc atatggctgc cgcgcggcac caggccgctg
  361 ctgtgatgat gatgatgatg gctgctgccc atggtatatc tccttcttaa agttaaacaa
  421 aattatttct agaggggaat tgttatccgc tcacaattcc cctatagtga gtcgtattaa
  481 tttcgcggga tcgagatctc gatcctctac gccggacgca tcgtggccgg catcaccggc
  541 gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct
  601 cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc
  661 gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac
  721 ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga
  781 gatcccggac accatcgaat ggcgcaaaac ctttcgcggt atggcatgat agcgcccgga
  841 agagagtcaa ttcagggtgg tgaatgtgaa accagtaacg ttatacgatg tcgcagagta
  901 tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac caggccagcc acgtttctgc
  961 gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg aattacattc ccaaccgcgt
 1021 ggcacaacaa ctggcgggca aacagtcgtt gctgattggc gttgccacct ccagtctggc
 1081 cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc
 1141 cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca
 1201 caatcttctc gcgcaacgcg tcagtgggct gatcattaac tatccgctgg atgaccagga
 1261 tgccattgct gtggaagctg cctgcactaa tgttccggcg ttatttcttg atgtctctga
 1321 ccagacaccc atcaacagta ttattttctc ccatgaagac ggtacgcgac tgggcgtgga
 1381 gcatctggtc gcattgggtc accagcaaat cgcgctgtta gcgggcccat taagttctgt
 1441 ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc actcgcaatc aaattcagcc
 1501 gatagcggaa cgggaaggcg actggagtgc catgtccggt tttcaacaaa ccatgcaaat
 1561 gctgaatgag ggcatcgttc ccactgcgat gctggttgcc aacgatcaga tggcgctggg
 1621 cgcaatgcgc gccattaccg agtccgggct gcgcgttggt gcggatatct cggtagtggg
 1681 atacgacgat accgaagaca gctcatgtta tatcccgccg ttaaccacca tcaaacagga
 1741 ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc agggccaggc
 1801 ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc
 1861 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
 1921 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtaagt tagctcactc
 1981 attaggcacc gggatctcga ccgatgccct tgagagcctt caacccagtc agctccttcc
 2041 ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac
 2101 tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc tttcgctgga
 2161 gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag
 2221 ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt atcgccggca
 2281 tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct
 2341 tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg caggccatgc
 2401 tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc gcggctctta
 2461 ccagcctaac ttcgatcact ggaccgctga tcgtcacggc gatttatgcc gcctcggcga
 2521 gcacatggaa cgggttggca tggattgtag gcgccgccct ataccttgtc tgcctccccg
 2581 cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg aatggaagcc ggcggcacct
 2641 cgctaacgga ttcaccactc caagaattgg agccaatcaa ttcttgcgga gaactgtgaa
 2701 tgcgcaaacc aacccttggc agaacatatc catcgcgtcc gccatctcca gcagccgcac
 2761 gcggcgcatc tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc
 2821 gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg aatcaccgat
 2881 acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag caacaacatg
 2941 aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag cgccctgcac
 3001 cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc
 3061 tgtattaacg aagcgctggc attgaccctg agtgattttt ctctggtccc gccgcatcca
 3121 taccgccagt tgtttaccct cacaacgttc cagtaaccgg gcatgttcat catcagtaac
 3181 ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt acccccatga acagaaatcc
 3241 cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt
 3301 tatcagaagc cagacattaa cgcttctgga gaaactcaac gagctggacg cggatgaaca
 3361 ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca gctgcctcgc
 3421 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
 3481 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
 3541 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt
 3601 aactatgcgg catcagagca gattgtactg agagtgcacc atatatgcgg tgtgaaatac
 3661 cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg
 3721 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
 3781 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
 3841 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
 3901 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
 3961 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
 4021 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
 4081 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
 4141 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
 4201 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
 4261 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
 4321 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
 4381 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
 4441 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
 4501 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
 4561 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
 4621 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
 4681 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
 4741 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
 4801 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
 4861 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
 4921 cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt
 4981 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
 5041 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
 5101 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
 5161 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
 5221 gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata
 5281 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
 5341 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
 5401 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
 5461 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
 5521 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
 5581 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
 5641 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
 5701 ttcaagaa
//

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