| 质粒名称: | pLVX-puro_G-CEPIA1er |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 164590 |
| 存储实验室: | Joseph Hyser |
|
载体骨架基本信息 |
|
| 载体名称: | pLVX-puro |
| 载体出品公司: | Clontech |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 8037 bp |
| 完整质粒大小: | 9535 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | G-CEPIA1er |
| 物种来源: | Synthetic |
| 插入大小: | 1497 bp |
| 基因突变: | G-GECO1.1 E299D M304L D328N N345K F360W G369S E372D V395E D401E |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | Myc（插入件上的C端子） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | XhoI（在克隆过程中被破坏） |
| 3' 克隆位点: | XbaI (未破坏) |
| 5' 测序引物 | CMV Forward |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pLVX-puro_G-CEPIA1er was a gift from Joseph Hyser (Addgene plasmid # 164590 ; http://n2t.net/addgene:164590 ; RRID:Addgene_164590) |
| 参考文献部分: | Rotavirus Calcium Dysregulation Manifests as Dynamic Calcium Signaling in the Cytoplasm and Endoplasmic Reticulum. Chang-Graham AL, Perry JL, Strtak AC, Ramachandran NK, Criglar JM, Philip AA, Patton JT, Estes MK, Hyser JM. Sci Rep. 2019 Jul 25;9(1):10822. doi: 10.1038/s41598-019-46856-8. 10.1038/s41598-019-46856-8 PubMed 31346185 |
买家导航
pLVX-puro_G-CEPIA1er载体质粒基本信息
pLVX-puro_G-CEPIA1er质粒图谱载体图谱和pLVX-puro_G-CEPIA1er载体序列质粒序列多克隆位点信息
pLVX-puro_G-CEPIA1er质粒载体简介
pLVX-puro_G-CEPIA1er质粒序列载体序列
LOCUS pLVX-puro_G-CEPIA1er 9488 bp ds-DNA circular SYN 13-MAY-2021
DEFINITION Stable integration and expression of G-CEPIA1er in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pLVX-puro_G-CEPIA1er
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9488)
AUTHORS Chang-Graham AL, Perry JL, Strtak AC, Ramachandran NK, Criglar JM,
Philip AA, Patton JT, Estes MK, Hyser JM
TITLE Rotavirus Calcium Dysregulation Manifests as Dynamic Calcium
Signaling in the Cytoplasm and Endoplasmic Reticulum.
JOURNAL Sci Rep. 2019 Jul 25;9(1):10822. doi: 10.1038/s41598-019-46856-8.
PUBMED 31346185
REFERENCE 2 (bases 1 to 9488)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9488
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..634
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 681..806
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1303..1536
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1721..1765
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2028..2143
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1 (lacking the first T)"
enhancer 2201..2504
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2505..2708
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 2659..2679
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 2705..2729
/label=LNCX
/note="Human CMV promoter, forward primer"
regulatory 2821..2830
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
sig_peptide join(2827..2871,2954..2965)
/product="modified signal sequence from the mouse
immunoglobulin heavy chain variable region"
/label=IgH signal sequence
CDS 3002..3058
/codon_start=1
/product="calmodulin-binding peptide from avian smooth
muscle myosin light chain kinase"
/label=calmodulin-binding peptide
/translation="RRKWNKTGHAVRAIGRLSS"
primer_bind 3271..3292
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind complement(3659..3678)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
CDS 3794..4234
/codon_start=1
/product="calcium-binding protein"
/label=calmodulin
/translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKDLGTVLRSLGQNPTEA
ELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVWDKDGNGYISAADL
RHVMTNLGEKLTDEEVDEMIREADIDGEGQVNYEEFVQMMTAK"
CDS 4244..4273
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
promoter 4323..4822
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(4358..4381)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 4755..4774
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
CDS 4843..5442
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(4843..4862)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 5339..5359
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 5456..6044
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(5509..5529)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5927..5938)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 6251..6884
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
primer_bind complement(7013..7029)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7013..7029)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7026..7048)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7037..7053
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7061..7091)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7106..7127
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7244..7261)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7415..8003)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7495..7514)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8174..9034)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8797..8816
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9035..9139)
/gene="bla"
/label=AmpR promoter
polyA_signal 9187..9321
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(9224..9243)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 9278..9297
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
2821 gccaccatgg gatggagctg tatcatcctc ttcttggtag caacagctac aggtaagggg
2881 ttaacagtag caggcttgag gtctggacat atatatgggt gacaatgaca tccactttgc
2941 ctttctctcc acaggcgcgc actcccaggt ccaactgcag ggatccatgg tcgactcttc
3001 acgtcgtaag tggaataaga caggtcacgc agtcagagct ataggtcggc tgagctcact
3061 cgagaacgtc tatatcaagg ccgacgagca gaagaacggc atcaaggcgt acttcaagat
3121 ccgccacaac atcgagggcg gcggcgtgca gctcgcctac cactaccagc agaacacccc
3181 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcgtgc agtccatact
3241 ttcgaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
3301 cgggatcact ctcggcatgg acgagctgta caagggcggt accggaggga gcatggtgag
3361 caagggcgag gagctgttca ccggggtggt gcccatccag gtcgagctgg acggcgacgt
3421 aaacggccac aagttcagcg tgtccggcga gggtgagggc gatgccacct acggcaagct
3481 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
3541 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
3601 cttcttcaag tccgccatgc ccgaaggcta catccaggag cgcaccatct tcttcaagga
3661 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
3721 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
3781 gtacaacacg cgtgaccaac tgactgaaga gcagatcgca gaatttaaag aggctttctc
3841 cctatttgac aaggacgggg atgggacgat aacaaccaag gatctgggga cggtgctgcg
3901 gtctctgggg cagaacccca cagaagcaga gctccaggac atgatcaatg aagtagatgc
3961 cgacggtaat ggcacaatcg acttccctga gttcctgaca atgatggcaa gaaaaatgaa
4021 agacacagac agtgaagaag aaattcgcga agcgttccgt gtgtgggata aggatggcaa
4081 tggctacatc tctgcagcag accttcgcca cgtgatgaca aaccttggag agaagttaac
4141 agatgaagag gttgatgaaa tgatcaggga agcagatatc gatggagaag gtcaggtaaa
4201 ctacgaagag tttgtacaaa tgatgacagc gaaggcggcc gcagaacaaa aactcatctc
4261 agaagaggat ctgaatgggg ccgcaagcga gaaggacgag ctgtagtcta gataattcta
4321 ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct
4381 gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag
4441 gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt
4501 caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc
4561 acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct
4621 ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag
4681 gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc
4741 ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc
4801 ttcctcatct ccgggccttt cgacctgcag cccaagctta ccatgaccga gtacaagccc
4861 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
4921 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
4981 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
5041 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
5101 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
5161 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
5221 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
5281 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
5341 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
5401 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaccgcgtct ggaacaatca
5461 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
5521 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
5581 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
5641 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
5701 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
5761 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
5821 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg
5881 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
5941 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
6001 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttaattctgc
6061 agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct
6121 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag
6181 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
6241 aagaggggac tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg
6301 atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc
6361 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta
6421 gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg
6481 gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac
6541 gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag
6601 ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga
6661 gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga
6721 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
6781 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
6841 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
6901 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggccttg
6961 acattgctag cgttttaccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
7021 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
7081 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
7141 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
7201 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
7261 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
7321 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
7381 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
7441 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
7501 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
7561 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
7621 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
7681 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
7741 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
7801 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
7861 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
7921 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
7981 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
8041 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
8101 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
8161 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
8221 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
8281 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
8341 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
8401 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
8461 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
8521 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
8581 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
8641 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
8701 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
8761 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
8821 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
8881 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
8941 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
9001 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
9061 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
9121 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
9181 gagatcaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
9241 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
9301 gtatcttatc atgtctggat caactggata actcaagcta accaaaatca tcccaaactt
9361 cccaccccat accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt
9421 gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac
9481 ttagtagt
//
