| 出品公司: | Agilent |
|---|---|
| 载体名称: | pTRV2-Gateway |
| 质粒类型: | 植物 |
| 质粒用途: | 干扰沉默 |
| 启动子: | PUC |
| 载体大小: | 3.0kb |
| 载体抗性: | KAN |
| 克隆菌株: | DB3.1 |
| 宿主细胞（系）: | shRNA,Gateway 空载体 |
| 产品目录号: | #1211 |
买家导航
pTRV2-Gateway载体质粒基本信息
pTRV2-Gateway质粒图谱载体图谱和pTRV2-Gateway载体序列质粒序列多克隆位点信息
pTRV2-Gateway质粒载体简介
pTRV2-Gateway质粒序列载体序列
LOCUS pTRV2-GFP 3092 bp DNA circular SYN 10-JUL-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3092)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 10, 2024 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..3092
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS complement(371..1021)
/codon_start=1
/gene="tetR"
/product="tetracycline resistance regulatory protein"
/label=TetR
/translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
misc_feature 1486..1510
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
promoter 1634..1813
/label=NOS promoter
/note="nopaline synthase promoter"
promoter 1666..1849
/function="NOS promoter"
/label=NOS promoter
/note="nopaline synthase promoter"
CDS 1870..2664
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
ORIGIN
1 gagcctatgg aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
61 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
121 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
181 gaggaagcgg aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg
241 acgctagggc agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg
301 aaagggggag gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca
361 gcggtcctga tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc
421 ttttcgccaa tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg
481 cttcgtcgaa ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg
541 tgccgccgcg ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag
601 tgaagtagct gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc
661 agaggaagcg aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg
721 catggatgcg cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc
781 cgatcagaaa tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg
841 ccagcatggc ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa
901 gcgccggctg ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc
961 cgacctcgtt caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca
1021 tgcttgacac tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa
1081 tccgcgcgtt caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat
1141 acccctgatc gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat
1201 gccggtgctg ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta
1261 tggcattctg ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc
1321 gctgtcggat cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc
1381 tggggaaccc tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc
1441 ccttttaaat atccgattat tctaataaac gctcttttct cttaggttta cccgccaata
1501 tatcctgtca aacactgata gtttaaactg aaggcgggaa acgacaatct gatcatgagc
1561 ggagaattaa gggagtcacg ttatgacccc cgccgatgac gcgggacaag ccgttttacg
1621 tttggaactg acagaaccgc aacgttgaag gagccactca gccgcgggtt tctggagttt
1681 aatgagctaa gcacatacgt cagaaaccat tattgcgcgt tcaaaagtcg cctaaggtca
1741 ctatcagcta gcaaatattt cttgtcaaaa atgctccact gacgttccat aaattcccct
1801 cggtatccaa ttagagtctc atattcactc tcaatccaaa taatctgcac cggatctgga
1861 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
1921 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
1981 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
2041 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
2101 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
2161 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
2221 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
2281 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
2341 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
2401 atgcccgacg gcgatgatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
2461 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
2521 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
2581 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
2641 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga
2701 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
2761 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
2821 agttcttcgc ccacgggatc tctgcggaac aggcggtcga aggtgccgat atcattacga
2881 cagcaacggc cgacaagcac aacgccacga tcctgagcga caatatgatc gggcccggcg
2941 tccacatcaa cggcgtcggc ggcgactgcc caggcaagac cgagatgcac cgcgatatct
3001 tgctgcgttc ggatattttc gtggagttcc cgccacagac ccggatgatc cccgatcgtt
3061 caaacattgg caataaagct caattcgacc cc
//
