pcDNA3.1/Myc-His C

价格:1350元

联系方式:I47-825O-882O

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pcDNA3.1/Myc-HisC载体基本信息

出品公司: Invitrogen
载体名称: pcDNA3.1/Myc-His C , pcDNA3.1/Myc-HisC
质粒类型: 哺乳动物细胞表达载体
启动子: CMV
表达水平:
克隆方法: 多克隆位点,限制性内切酶
载体大小: 5490 bp
5' 测序引物:   pcDNA3.1-F:CTAGAGAACCCACTGCTTAC
3' 测序引物:   pcDNA3.1-R (BGH-R):TAGAAGGCACAGTCGAGG
载体标签: C-His, C-Myc
载体抗性: Ampicillin 氨苄
筛选标记: Neomycin (G418)
备注:
 
产品目录号: V855-20
稳定性: 瞬表达 Transient
组成型: 组成型 Constitutive
病毒/非病毒: 非病毒

pcDNA3.1/Myc-HisC载体质粒图谱和多克隆位点信息

pcDNA3.1/Myc-His c载体图谱

pcDNA3.1/Myc-His B多克隆位点

pcDNA3.1/Myc-HisC载体简介

pcDNA3.1/myc-His(-) A, B, and C are 5.5 kb vectors designed for overproduction of recombinant proteins in mammalian cell lines. The vectors are supplied in three reading frames to facilitate in frame cloning, with a C-terminal peptide containing a polyhistidine metal-binding tag and the myc (c-myc) epitope. The human cytomegalovirus immediate-early (CMV) promoter provides highlevel expression in a wide range of mammalian cells. In addition, the vector will replicate episomally in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. COS7). High-level stable and nonreplicative transient expression can be carried out in most mammalian cells. The control plasmid, pcDNA3.1/myc-His(-)/lacZ, is the pcDNA3.1/myc-His(-) A vector with a 3.2 kb fragment containing the β-galactosidase gene cloned in frame with the C-terminal peptide. It is included for use as a positive control for transfection, expression, and detection in the cell line of choice.

pcDNA3.1/Myc-HisC载体序列

LOCUS       pcDNA3.1(+)/myc-HisC       5490 bp    DNA     circular SYN  2-SEP-2003
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..5490
                     /invitrogen="128"
                     /vntifkey="98"
     CDS             1038..1055
                     /invitrogen="1010000"
                     /vntifkey="4"
                     /label=6xHis
     CDS             complement(4494..5354)
                     /invitrogen="1020000"
                     /vntifkey="4"
                     /label=Amp(R)
     polyA_site      1084..1308
                     /invitrogen="1870000"
                     /vntifkey="26"
                     /label=BGH\pA
     primer          complement(1078..1095)
                     /invitrogen="1910000"
                     /vntifkey="27"
                     /label=BGH\reverse\primer
     promoter        complement(5355..5453)
                     /invitrogen="2330000"
                     /vntifkey="30"
                     /label=bla\promoter
     primer          769..789
                     /invitrogen="1930000"
                     /vntifkey="27"
                     /label=CMV\forward\primer
     promoter        232..819
                     /invitrogen="2180000"
                     /vntifkey="29"
                     /label=CMV\promoter
     CDS             993..1022
                     /invitrogen="1090000"
                     /vntifkey="4"
                     /label=c-myc\epitope
     rep_origin      1354..1782
                     /invitrogen="2500000"
                     /vntifkey="33"
                     /label=f1\origin
     misc_feature    902..995
                     /invitrogen="1470000"
                     /vntifkey="21"
                     /label=MCS
     CDS             2193..2987
                     /invitrogen="1260000"
                     /vntifkey="4"
                     /label=Neo(R)
     rep_origin      complement(3676..4349)
                     /invitrogen="2540000"
                     /vntifkey="33"
                     /label=pUC\origin
     promoter        1787..2157
                     /invitrogen="2290000"
                     /vntifkey="29"
                     /label=SV40\early\promoter
     polyA_signal    3163..3293
                     /invitrogen="1840000"
                     /vntifkey="25"
                     /label=SV40\pA
     primer          863..882
                     /invitrogen="2100000"
                     /vntifkey="27"
                     /label=T7\primer
     promoter        863..879
                     /invitrogen="2370000"
                     /vntifkey="30"
                     /label=T7\promoter
BASE COUNT     1273 a      1427 c      1397 g      1393 t
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttggt accgagctcg gatccactag tccagtgtgg tggaattctg cagatatcca
      961 gcacagtggc ggccgctcga ggtcacccat tcgaacaaaa actcatctca gaagaggatc
     1021 tgaatatgca taccggtcat catcaccatc accattgagt ttaaacccgc tgatcagcct
     1081 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     1141 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     1201 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     1261 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     1321 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     1381 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     1441 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     1501 taaatcgggg catcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     1561 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     1621 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     1681 tcaaccctat ctcggtctat tcttttgatt tataagggat tttggggatt tcggcctatt
     1741 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     1801 tcagttaggg tgtggaaagt ccccaggctc cccaggcagg cagaagtatg caaagcatgc
     1861 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     1921 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     1981 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     2041 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
     2101 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
     2161 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     2221 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     2281 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     2341 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     2401 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     2461 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     2521 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     2581 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     2641 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     2701 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
     2761 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     2821 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     2881 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     2941 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     3001 gttcgcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
     3061 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     3121 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     3181 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     3241 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     3301 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     3361 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     3421 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     3481 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     3541 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     3601 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     3661 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     3721 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     3781 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     3841 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     3901 ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg
     3961 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     4021 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     4081 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     4141 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     4201 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     4261 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     4321 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     4381 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     4441 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     4501 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     4561 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     4621 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     4681 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     4741 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     4801 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     4861 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     4921 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     4981 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     5041 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     5101 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     5161 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     5221 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     5281 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     5341 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     5401 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     5461 acatttcccc gaaaagtgcc acctgacgtc
//

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