pCas

价格:1000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Addgene
载体名称: pCas
质粒类型: CRISPR/Cas9载体;基因敲除载体;原核载体
高拷贝/低拷贝: 低拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: native cas9 promoter
载体大小: 12545 bp
5' 测序引物及序列: pENTR1A-3 (GTAACATCAGAGATTTTGAGACAC)
3' 测序引物及序列: AraC-F-New (GATGTAGCCGTCAAGTTGTCA)
载体标签: NLS、His8 (C ter)
载体抗性: Kanamycin.html' target='_blank'>卡那霉素(Kanamycin)
筛选标记: --
克隆菌株: Stbl3
宿主细胞(系): 细菌
备注: Must be grown at 30C! Vector has temperature-sensitive replication (RepA101ts)
产品目录号: #62225
稳定性: 稳表达
组成型/诱导型: 诱导型
病毒/非病毒: 非病毒

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载体质粒图谱和多克隆位点信息

pCas 载体图谱



载体简介

载体序列

LOCUS       pCas	12545 bp 	DNA	SYN
DEFINITION  pCas
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..12545
                     /organism="pCas"
                     /mol_type="other DNA"
     misc_feature    complement(404..1495)
                     /label="lacI"
     CDS             complement(404..1363)
                     /label="ORF frame 2"
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                     SGLRVGADISVVGYDDTEDSSCYIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
     CDS             complement(1698..2648)
                     /label="ORF frame 1"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRK
                     ERTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAK
                     FSSEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHK
                     ANIEISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTL
                     IFQVELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTS
                     FEAKFLSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI*"
     gene            complement(3572..4387)
                     /label="KanR2"
                     /gene="KanR2"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRK
                     ERTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAK
                     FSSEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHK
                     ANIEISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTL
                     IFQVELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTS
                     FEAKFLSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI*"
     CDS             complement(3572..4387)
                     /label="ORF frame 2"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
     CDS             4917..9023
                     /label="ORF frame 3"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDDYKVPSKKFKVLGNTDRHSIK
                     KNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
                     ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAH
                     MIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSK
                     SRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLD
                     NLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTL
                     LKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVK
                     LNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY
                     YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVL
                     PKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKE
                     DYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLT
                     LFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDF
                     LKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQT
                     VKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKE
                     HPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKV
                     LTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKA
                     GFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIG
                     KATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
                     MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVV
                     AKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLF
                     ELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQH
                     KHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGA
                     PAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD*"
     misc_feature    complement(9153..10031)
                     /label="AraC"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDDYKVPSKKFKVLGNTDRHSIK
                     KNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
                     ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAH
                     MIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSK
                     SRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLD
                     NLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTL
                     LKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVK
                     LNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY
                     YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVL
                     PKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKE
                     DYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLT
                     LFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDF
                     LKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQT
                     VKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKE
                     HPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKV
                     LTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKA
                     GFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIG
                     KATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
                     MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVV
                     AKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLF
                     ELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQH
                     KHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGA
                     PAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD*"
     CDS             complement(9153..10082)
                     /label="ORF frame 1"
                     /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE
                     ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH
                     PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG
                     EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA
                     QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL
                     YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*"
     misc_feature    10061..10076
                     /label="araO2"
                     /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE
                     ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH
                     PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG
                     EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA
                     QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL
                     YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*"
     misc_feature    10218..10229
                     /label="araO1"
                     /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE
                     ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH
                     PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG
                     EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA
                     QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL
                     YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*"
     misc_feature    10260..10273
                     /label="CAP_BS"
                     /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE
                     ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH
                     PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG
                     EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA
                     QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL
                     YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*"
     misc_feature    10265..10284
                     /label="pBAD_fwd_primer"
                     /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE
                     ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH
                     PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG
                     EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA
                     QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL
                     YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*"
     misc_feature    10270..10308
                     /label="AraI1I2"
                     /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE
                     ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH
                     PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG
                     EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA
                     QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL
                     YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*"
     promoter        10305..10333
                     /label="ARA_promoter"
                     /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE
                     ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH
                     PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG
                     EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA
                     QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL
                     YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*"
     CDS             10808..11593
                     /label="ORF frame 2"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA*"
     CDS             11590..12270
                     /label="ORF frame 1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKNAWYFANYDPRMKREGLHYVVIERDEKYMASFDEIVPEFIEKMDEALAEIG
                     FVFGEQWR*"
ORIGIN
    1 gatctcaaaa aaagcaccga ctcggtgcca ctttttcaag ttgataacgg actagcctta
   61 ttttaacttg ctatttctag ctctaaaacc tggtaacagg attagcagat gtgtgaaatt
  121 gttatccgct cacaattcca cacattatac gagccggatg attaattgtc aacagctcat
  181 ttcagaatat ttgccagaac cgttatgatg tcggcgcaaa aaacattatc cagaacggga
  241 gtgcgccttg agcgacacga attatgcagt gatttacgac ctgcacagcc ataccacagc
  301 ttccgatggc tgcctgacgc cagaagcatt ggtgcaccgt gcagtcgatg ataagctgtc
  361 aaaccagatc aattcgcgct aactcacatt aattgcgttg cgctcactgc ccgctttcca
  421 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
  481 tttgcgtatt gggcgccagg gtggtttttc ttttcaccag tgagacgggc aacagctgat
  541 tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg gtttgcccca
  601 gcaggcgaaa atcctgtttg atggtggttg acggcgggat ataacatgag ctgtcttcgg
  661 tatcgtcgta tcccactacc gagatatccg caccaacgcg cagcccggac tcggtaatgg
  721 cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag catcgcagtg ggaacgatgc
  781 cctcattcag catttgcatg gtttgttgaa aaccggacat ggcactccag tcgccttccc
  841 gttccgctat cggctgaatt tgattgcgag tgagatattt atgccagcca gccagacgca
  901 gacgcgccga gacagaactt aatgggcccg ctaacagcgc gatttgctgg tgacccaatg
  961 cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata atactgttga
 1021 tgggtgtctg gtcagagaca tcaagaaata acgccggaac attagtgcag gcagcttcca
 1081 cagcaatggc atcctggtca tccagcggat agttaatgat cagcccactg acgcgttgcg
 1141 cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct accatcgaca
 1201 ccaccacgct ggcacccagt tgatcggcgc gagatttaat cgccgcgaca atttgcgacg
 1261 gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag caacgactgt ttgcccgcca
 1321 gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc catcgccgct tccacttttt
 1381 cccgcgtttt cgcagaaacg tggctggcct ggttcaccac gcgggaaacg gtctgataag
 1441 agacaccggc atactctgcg acatcgtata acgttactgg tttcacattc accaccctga
 1501 attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcac cattcgatgg
 1561 tgtcaacgta aatgcatgcc gcttcgcctt ccatgggtat ggacagtttt ccctttgata
 1621 tgtaacggtg aacagttgtt ctacttttgt ttgttagtct tgatgcttca ctgatagata
 1681 caagagccat aagaacctca gatccttccg tatttagcca gtatgttctc tagtgtggtt
 1741 cgttgttttt gcgtgagcca tgagaacgaa ccattgagat catacttact ttgcatgtca
 1801 ctcaaaaatt ttgcctcaaa actggtgagc tgaatttttg cagttaaagc atcgtgtagt
 1861 gtttttctta gtccgttacg taggtaggaa tctgatgtaa tggttgttgg tattttgtca
 1921 ccattcattt ttatctggtt gttctcaagt tcggttacga gatccatttg tctatctagt
 1981 tcaacttgga aaatcaacgt atcagtcggg cggcctcgct tatcaaccac caatttcata
 2041 ttgctgtaag tgtttaaatc tttacttatt ggtttcaaaa cccattggtt aagcctttta
 2101 aactcatggt agttattttc aagcattaac atgaacttaa attcatcaag gctaatctct
 2161 atatttgcct tgtgagtttt cttttgtgtt agttctttta ataaccactc ataaatcctc
 2221 atagagtatt tgttttcaaa agacttaaca tgttccagat tatattttat gaattttttt
 2281 aactggaaaa gataaggcaa tatctcttca ctaaaaacta attctaattt ttcgcttgag
 2341 aacttggcat agtttgtcca ctggaaaatc tcaaagcctt taaccaaagg attcctgatt
 2401 tccacagttc tcgtcatcag ctctctggtt gctttagcta atacaccata agcattttcc
 2461 ctactgatgt tcatcatctg agcgtattgg ttataagtga acgataccgt ccgttctttc
 2521 cttgtagggt tttcaatcgt ggggttgagt agtgccacac agcataaaat tagcttggtt
 2581 tcatgctccg ttaagtcata gcgactaatc gctagttcat ttgctttgaa aacaactaat
 2641 tcagacatac atctcaattg gtctaggtga ttttaatcac tataccaatt gagatgggct
 2701 agtcaatgat aattactagt ccttttcctt tgagttgtgg gtatctgtaa attctgctag
 2761 acctttgctg gaaaacttgt aaattctgct agaccctctg taaattccgc tagacctttg
 2821 tgtgtttttt ttgtttatat tcaagtggtt ataatttata gaataaagaa agaataaaaa
 2881 aagataaaaa gaatagatcc cagccctgtg tataactcac tactttagtc agttccgcag
 2941 tattacaaaa ggatgtcgca aacgctgttt gctcctctac aaaacagacc ttaaaaccct
 3001 aaaggcttaa gtagcaccct cgcaagctcg gttgcggccg caatcgggca aatcgctgaa
 3061 tattcctttt gtctccgacc atcaggcacc tgagtcgctg tctttttcgt gacattcagt
 3121 tcgctgcgct cacggctctg gcagtgaatg ggggtaaatg gcactacagg cgccttttat
 3181 ggattcatgc aaggaaacta cccataatac aagaaaagcc cgtcacgggc ttctcagggc
 3241 gttttatggc gggtctgcta tgtggtgcta tctgactttt tgctgttcag cagttcctgc
 3301 cctctgattt tccagtctga ccacttcgga ttatcccgtg acaggtcatt cagactggct
 3361 aatgcaccca gtaaggcagc ggtatcatca acggggtctg acgctcagtg gaacgaaaac
 3421 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
 3481 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
 3541 tacgtttcca caaccaatta accaattctg attagaaaaa ctcatcgagc atcaaatgaa
 3601 actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta
 3661 atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg
 3721 cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt
 3781 tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagcttat
 3841 gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg
 3901 catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc
 3961 tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg
 4021 catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat gctgttttcc
 4081 cggggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg
 4141 tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat
 4201 tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca
 4261 atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata
 4321 aatcagcatc catgttggaa tttaatcgcg gcctcgagca agacgtttcc cgttgaatat
 4381 ggctcataac accccttgta ttactgttta tgtaagcaga cagttttatt gttcatgatg
 4441 atatattttt atcttgtgca atgtaacatc agagattttg agacacaacg tggctttccc
 4501 tgcagggttt gcagtcagag tagaatagaa gtatcaaaaa aagcaccgac tcggtgccac
 4561 tttttcaagt tgataacgga ctagccttat tttaacttgc tatgctgttt tgaatggttc
 4621 caacaagatt attttataac ttttataaca aataatcaag gagaaattca aagaaattta
 4681 tcagccataa aacaatactt aatactatag aatgataaca aaataaacta ctttttaaaa
 4741 gaattttgtg ttataatcta tttattatta agtattgggt aatatttttt gaagagatat
 4801 tttgaaaaag aaaaattaaa gcatattaaa ctaatttcgg aggtcattaa aactattatt
 4861 gaaatcatca aactcattat ggatttaatt taaacttttt attttaggag gcaaaaatgg
 4921 ataagaaata ctcaataggc ttagatatcg gcacaaatag cgtcggatgg gcggtgatca
 4981 ctgatgatta taaggttccg tctaaaaagt tcaaggttct gggaaataca gaccgccaca
 5041 gtatcaaaaa aaatcttata ggggctcttt tatttgacag tggagagaca gcggaagcga
 5101 ctcgtctcaa acggacagct cgtagaaggt atacacgtcg gaagaatcgt atttgttatc
 5161 tacaggagat tttttcaaat gagatggcga aagtagatga tagtttcttt catcgacttg
 5221 aagagtcttt tttggtggaa gaagacaaga agcatgaacg tcatcctatt tttggaaata
 5281 tagtagatga agttgcttat catgagaaat atccaactat ctatcatctg cgaaaaaaat
 5341 tggtagattc tactgataaa gcggatttgc gcttaatcta tttggcctta gcgcatatga
 5401 ttaagtttcg tggtcatttt ttgattgagg gagatttaaa tcctgataat agtgatgtgg
 5461 acaaactatt tatccagttg gtacaaacct acaatcaatt atttgaagaa aaccctatta
 5521 acgcaagtgg agtagatgct aaagcgattc tttctgcacg attgagtaaa tcaagacgat
 5581 tagaaaatct cattgctcag ctccccggtg agaagaaaaa tggcttattt gggaatctca
 5641 ttgctttgtc attgggtttg acccctaatt ttaaatcaaa ttttgatttg gcagaagatg
 5701 ctaaattaca gctttcaaaa gatacttacg atgatgattt agataattta ttggcgcaaa
 5761 ttggagatca atatgctgat ttgtttttgg cagctaagaa tttatcagat gctattttac
 5821 tttcagatat cctaagagta aatactgaaa taactaaggc tcccctatca gcttcaatga
 5881 ttaaacgcta cgatgaacat catcaagact tgactctttt aaaagcttta gttcgacaac
 5941 aacttccaga aaagtataaa gaaatctttt ttgatcaatc aaaaaacgga tatgcaggtt
 6001 atattgatgg gggagctagc caagaagaat tttataaatt tatcaaacca attttagaaa
 6061 aaatggatgg tactgaggaa ttattggtga aactaaatcg tgaagatttg ctgcgcaagc
 6121 aacggacctt tgacaacggc tctattcccc atcaaattca cttgggtgag ctgcatgcta
 6181 ttttgagaag acaagaagac ttttatccat ttttaaaaga caatcgtgag aagattgaaa
 6241 aaatcttgac ttttcgaatt ccttattatg ttggtccatt ggcgcgtggc aatagtcgtt
 6301 ttgcatggat gactcggaag tctgaagaaa caattacccc atggaatttt gaagaagttg
 6361 tcgataaagg tgcttcagct caatcattta ttgaacgcat gacaaacttt gataaaaatc
 6421 ttccaaatga aaaagtacta ccaaaacata gtttgcttta tgagtatttt acggtttata
 6481 acgaattgac aaaggtcaaa tatgttactg aaggaatgcg aaaaccagca tttctttcag
 6541 gtgaacagaa gaaagccatt gttgatttac tcttcaaaac aaatcgaaaa gtaaccgtta
 6601 agcaattaaa agaagattat ttcaaaaaaa tagaatgttt tgatagtgtt gaaatttcag
 6661 gagttgaaga tagatttaat gcttcattag gtacctacca tgatttgcta aaaattatta
 6721 aagataaaga ttttttggat aatgaagaaa atgaagatat cttagaggat attgttttaa
 6781 cattgacctt atttgaagat agggagatga ttgaggaaag acttaaaaca tatgctcacc
 6841 tctttgatga taaggtgatg aaacagctta aacgtcgccg ttatactggt tggggacgtt
 6901 tgtctcgaaa attgattaat ggtattaggg ataagcaatc tggcaaaaca atattagatt
 6961 ttttgaaatc agatggtttt gccaatcgca attttatgca gctgatccat gatgatagtt
 7021 tgacatttaa agaagacatt caaaaagcac aagtgtctgg acaaggcgat agtttacatg
 7081 aacatattgc aaatttagct ggtagccctg ctattaaaaa aggtatttta cagactgtaa
 7141 aagttgttga tgaattggtc aaagtaatgg ggcggcataa gccagaaaat atcgttattg
 7201 aaatggcacg tgaaaatcag acaactcaaa agggccagaa aaattcgcga gagcgtatga
 7261 aacgaatcga agaaggtatc aaagaattag gaagtcagat tcttaaagag catcctgttg
 7321 aaaatactca attgcaaaat gaaaagctct atctctatta tctccaaaat ggaagagaca
 7381 tgtatgtgga ccaagaatta gatattaatc gtttaagtga ttatgatgtc gatcacattg
 7441 ttccacaaag tttccttaaa gacgattcaa tagacaataa ggtcttaacg cgttctgata
 7501 aaaatcgtgg taaatcggat aacgttccaa gtgaagaagt agtcaaaaag atgaaaaact
 7561 attggagaca acttctaaac gccaagttaa tcactcaacg taagtttgat aatttaacga
 7621 aagctgaacg tggaggtttg agtgaacttg ataaagctgg ttttatcaaa cgccaattgg
 7681 ttgaaactcg ccaaatcact aagcatgtgg cacaaatttt ggatagtcgc atgaatacta
 7741 aatacgatga aaatgataaa cttattcgag aggttaaagt gattacctta aaatctaaat
 7801 tagtttctga cttccgaaaa gatttccaat tctataaagt acgtgagatt aacaattacc
 7861 atcatgccca tgatgcgtat ctaaatgccg tcgttggaac tgctttgatt aagaaatatc
 7921 caaaacttga atcggagttt gtctatggtg attataaagt ttatgatgtt cgtaaaatga
 7981 ttgctaagtc tgagcaagaa ataggcaaag caaccgcaaa atatttcttt tactctaata
 8041 tcatgaactt cttcaaaaca gaaattacac ttgcaaatgg agagattcgc aaacgccctc
 8101 taatcgaaac taatggggaa actggagaaa ttgtctggga taaagggcga gattttgcca
 8161 cagtgcgcaa agtattgtcc atgccccaag tcaatattgt caagaaaaca gaagtacaga
 8221 caggcggatt ctccaaggag tcaattttac caaaaagaaa ttcggacaag cttattgctc
 8281 gtaaaaaaga ctgggatcca aaaaaatatg gtggttttga tagtccaacg gtagcttatt
 8341 cagtcctagt ggttgctaag gtggaaaaag ggaaatcgaa gaagttaaaa tccgttaaag
 8401 agttactagg gatcacaatt atggaaagaa gttcctttga aaaaaatccg attgactttt
 8461 tagaagctaa aggatataag gaagttaaaa aagacttaat cattaaacta cctaaatata
 8521 gtctttttga gttagaaaac ggtcgtaaac ggatgctggc tagtgccgga gaattacaaa
 8581 aaggaaatga gctggctctg ccaagcaaat atgtgaattt tttatattta gctagtcatt
 8641 atgaaaagtt gaagggtagt ccagaagata acgaacaaaa acaattgttt gtggagcagc
 8701 ataagcatta tttagatgag attattgagc aaatcagtga attttctaag cgtgttattt
 8761 tagcagatgc caatttagat aaagttctta gtgcatataa caaacataga gacaaaccaa
 8821 tacgtgaaca agcagaaaat attattcatt tatttacgtt gacgaatctt ggagctcccg
 8881 ctgcttttaa atattttgat acaacaattg atcgtaaacg atatacgtct acaaaagaag
 8941 ttttagatgc cactcttatc catcaatcca tcactggtct ttatgaaaca cgcattgatt
 9001 tgagtcagct aggaggtgac tgaagtatat tttagatgaa gattatttct taatctagac
 9061 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
 9121 tttccccgaa aagtgccacc tgcatcgatt tattatgaca acttgacggc tacatcattc
 9181 actttttctt cacaaccggc acggaactcg ctcgggctgg ccccggtgca ttttttaaat
 9241 acccgcgaga aatagagttg atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc
 9301 atccgggtgg tgctcaaaag cagcttcgcc tggctgatac gttggtcctc gcgccagctt
 9361 aagacgctaa tccctaactg ctggcggaaa agatgtgaca gacgcgacgg cgacaagcaa
 9421 acatgctgtg cgacgctggc gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac
 9481 tgacaagcct cgcgtacccg attatccatc ggtggatgga gcgactcgtt aatcgcttcc
 9541 atgcgccgca gtaacaattg ctcaagcaga tttatcgcca gcagctccga atagcgccct
 9601 tccccttgcc cggcgttaat gatttgccca aacaggtcgc tgaaatgcgg ctggtgcgct
 9661 tcatccgggc gaaagaaccc cgtattggca aatattgacg gccagttaag ccattcatgc
 9721 cagtaggcgc gcggacgaaa gtaaacccac tggtgatacc attcgcgagc ctccggatga
 9781 cgaccgtagt gatgaatctc tcctggcggg aacagcaaaa tatcacccgg tcggcaaaca
 9841 aattctcgtc cctgattttt caccaccccc tgaccgcgaa tggtgagatt gagaatataa
 9901 cctttcattc ccagcggtcg gtcgataaaa aaatcgagat aaccgttggc ctcaatcggc
 9961 gttaaacccg ccaccagatg ggcattaaac gagtatcccg gcagcagggg atcattttgc
10021 gcttcagcca tacttttcat actcccgcca ttcagagaag aaaccaattg tccatattgc
10081 atcagacatt gccgtcactg cgtcttttac tggctcttct cgctaaccaa accggtaacc
10141 ccgcttatta aaagcattct gtaacaaagc gggaccaaag ccatgacaaa aacgcgtaac
10201 aaaagtgtct ataatcacgg cagaaaagtc cacattgatt atttgcacgg cgtcacactt
10261 tgctatgcca tagcattttt atccataaga ttagcggatc ctacctgacg ctttttatcg
10321 caactctcta ctgtttctcc atacccgttt ttttgggaat tcgagctcta aggaggttat
10381 aaaaaatgga tattaatact gaaactgaga tcaagcaaaa gcattcacta accccctttc
10441 ctgttttcct aatcagcccg gcatttcgcg ggcgatattt tcacagctat ttcaggagtt
10501 cagccatgaa cgcttattac attcaggatc gtcttgaggc tcagagctgg gcgcgtcact
10561 accagcagct cgcccgtgaa gagaaagagg cagaactggc agacgacatg gaaaaaggcc
10621 tgccccagca cctgtttgaa tcgctatgca tcgatcattt gcaacgccac ggggccagca
10681 aaaaatccat tacccgtgcg tttgatgacg atgttgagtt tcaggagcgc atggcagaac
10741 acatccggta catggttgaa accattgctc accaccaggt tgatattgat tcagaggtat
10801 aaaacgaatg agtactgcac tcgcaacgct ggctgggaag ctggctgaac gtgtcggcat
10861 ggattctgtc gacccacagg aactgatcac cactcttcgc cagacggcat ttaaaggtga
10921 tgccagcgat gcgcagttca tcgcattact gatcgttgcc aaccagtacg gccttaatcc
10981 gtggacgaaa gaaatttacg cctttcctga taagcagaat ggcatcgttc cggtggtggg
11041 cgttgatggc tggtcccgca tcatcaatga aaaccagcag tttgatggca tggactttga
11101 gcaggacaat gaatcctgta catgccggat ttaccgcaag gaccgtaatc atccgatctg
11161 cgttaccgaa tggatggatg aatgccgccg cgaaccattc aaaactcgcg aaggcagaga
11221 aatcacgggg ccgtggcagt cgcatcccaa acggatgtta cgtcataaag ccatgattca
11281 gtgtgcccgt ctggccttcg gatttgctgg tatctatgac aaggatgaag ccgagcgcat
11341 tgtcgaaaat actgcataca ctgcagaacg tcagccggaa cgcgacatca ctccggttaa
11401 cgatgaaacc atgcaggaga ttaacactct gctgatcgcc ctggataaaa catgggatga
11461 cgacttattg ccgctctgtt cccagatatt tcgccgcgac attcgtgcat cgtcagaact
11521 gacacaggcc gaagcagtaa aagctcttgg attcctgaaa cagaaagccg cagagcagaa
11581 ggtggcagca tgacaccgga cattatcctg cagcgtaccg ggatcgatgt gagagctgtc
11641 gaacaggggg atgatgcgtg gcacaaatta cggctcggcg tcatcaccgc ttcagaagtt
11701 cacaacgtga tagcaaaacc ccgctccgga aagaagtggc ctgacatgaa aatgtcctac
11761 ttccacaccc tgcttgctga ggtttgcacc ggtgtggctc cggaagttaa cgctaaagca
11821 ctggcctggg gaaaacagta cgagaacgac gccagaaccc tgtttgaatt cacttccggc
11881 gtgaatgtta ctgaatcccc gatcatctat cgcgacgaaa gtatgcgtac cgcctgctct
11941 cccgatggtt tatgcagtga cggcaacggc cttgaactga aatgcccgtt tacctcccgg
12001 gatttcatga agttccggct cggtggtttc gaggccataa agtcagctta catggcccag
12061 gtgcagtaca gcatgtgggt gacgcgaaaa aatgcctggt actttgccaa ctatgacccg
12121 cgtatgaagc gtgaaggcct gcattatgtc gtgattgagc gggatgaaaa gtacatggcg
12181 agttttgacg agatcgtgcc ggagttcatc gaaaaaatgg acgaggcact ggctgaaatt
12241 ggttttgtat ttggggagca atggcgatga cgcatcctca cgataatatc cgggtaggcg
12301 caatcacttt cgtctactcc gttacaaagc gaggctgggt atttcccggc ctttctgtta
12361 tccgaaatcc actgaaagca cagcggctgg ctgaggagat aaataataaa cgaggggctg
12421 tatgcacaaa gcatcttctg ttgagttaag aacgagtatc gagatggcac atagccttgc
12481 tcaaattgga atcaggtttg tgccaatacc agtagaaaca gacgaagaat ccatgggtat
12541 ggaca
//

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