pSUPER.neo+gfp

LOCUS       pSUPER.neo+GFP	5429 bp 	DNA	SYN
DEFINITION  pSUPER.neo+GFP
ACCESSION   
KEYWORDS    
SOURCE       www.biofeng.com     
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..5429
                     /organism="pSUPER.neo+GFP"
                     /mol_type="other DNA"
     rep_origin      complement(135..441)
                     /label="f1_origin"
     misc_feature    complement(459..614)
                     /label="lacZ_a"
     misc_feature    585..607
                     /label="M13_pUC_fwd_primer"
     promoter        600..616
                     /label="M13_forward20_primer"
     promoter        626..644
                     /label="T7_promoter"
     misc_feature    677..693
                     /label="pBluescriptSK_primer"
     terminator      complement(731..779)
                     /label="synth_PA_terminator"
     CDS             792..1328
                     /label="ORF frame 3"                     
     CDS             complement(843..1637)
                     /label="ORF frame 1"                     
     CDS             complement(1680..2417)
                     /label="ORF frame 1"                     
     gene            complement(1701..2414)
                     /label="EGFP"
                     /gene="EGFP"                     
     misc_feature    complement(1743..1764)
                     /label="EGFP_C_primer"                     
     misc_feature    complement(2205..2234)
                     /label="Y66 (EGFP)"                     
     misc_feature    2351..2372
                     /label="EGFP_N_primer"                     
     misc_feature    complement(2500..2519)
                     /label="mPGK_F_primer"                     
     misc_feature    2897..2920
                     /label="MSCV_rev_primer"                    
     misc_feature    3101..3120
                     /label="H1_primer"                     
     misc_feature    complement(3196..3212)
                     /label="pBluescriptKS_primer"                     
     promoter        complement(3240..3259)
                     /label="T3_promoter"                     
     promoter        complement(3276..3294)
                     /label="M13_reverse_primer"                     
     misc_feature    complement(3293..3315)
                     /label="M13_pUC_rev_primer"                     
     promoter        complement(3329..3358)
                     /label="lac_promoter"                     
     gene            complement(4441..5301)
                     /label="Ampicillin"
                     /gene="Ampicillin"                     
     CDS             complement(4441..5301)
                     /label="ORF frame 3"                     
     promoter        complement(5343..5371)
                     /label="AmpR_promoter"                    
ORIGIN
    1 CTAAATTGTA AGCGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC
   61 ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA
  121 GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC
  181 CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC
  241 CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG
  301 CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA
  361 AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC
  421 CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCC CATTCGCCAT TCAGGCTGCG
  481 CAACTGTTGG GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA TTACGCCAGC TGGCGAAAGG
  541 GGGATGTGCT GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT CACGACGTTG
  601 TAAAACGACG GCCAGTGAGC GCGCGTAATA CGACTCACTA TAGGGCGAAT TGGAGCTCCA
  661 CCGCGGTGGC GGCCGCTCTA GAACTAGTGG ATCCCCCGGG CTGCATCCCC GATCCTTATC
  721 GCTATCGATT CACACAAAAA ACCAACACAC AGATGTAATG AAAATAAAGA TATTTTATTG
  781 CGGCCATCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC
  841 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG
  901 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA
  961 CGGGTAGCCA ACGCTATGTC CTGATAGCGG TCCGCCACAC CCAGCCGGCC ACAGTCGATG
 1021 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC
 1081 ACGACGAGAT CCTCGCCGTC GGGCATGCGC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC
 1141 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA
 1201 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA
 1261 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG
 1321 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT
 1381 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC
 1441 CGCGCTGCCT CGTCCTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA
 1501 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT
 1561 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT
 1621 CCATCTTGTT CAATCATGGT GGCTCTAGCC TTAAGTTCGA GACTGTTGTG TCAGAAGAAT
 1681 CAAGCTGATC TGAGTCCGGA CTTGTACAGC TCGTCCATGC CGAGAGTGAT CCCGGCGGCG
 1741 GTCACGAACT CCAGCAGGAC CATGTGATCG CGCTTCTCGT TGGGGTCTTT GCTCAGGGCG
 1801 GACTGGGTGC TCAGGTAGTG GTTGTCGGGC AGCAGCACGG GGCCGTCGCC GATGGGGGTG
 1861 TTCTGCTGGT AGTGGTCGGC GAGCTGCACG CTGCCGTCCT CGATGTTGTG GCGGATCTTG
 1921 AAGTTCACCT TGATGCCGTT CTTCTGCTTG TCGGCCATGA TATAGACGTT GTGGCTGTTG
 1981 TAGTTGTACT CCAGCTTGTG CCCCAGGATG TTGCCGTCCT CCTTGAAGTC GATGCCCTTC
 2041 AGCTCGATGC GGTTCACCAG GGTGTCGCCC TCGAACTTCA CCTCGGCGCG GGTCTTGTAG
 2101 TTGCCGTCGT CCTTGAAGAA GATGGTGCGC TCCTGGACGT AGCCTTCGGG CATGGCGGAC
 2161 TTGAAGAAGT CGTGCTGCTT CATGTGGTCG GGGTAGCGGC TGAAGCACTG CACGCCGTAG
 2221 GTCAGGGTGG TCACGAGGGT GGGCCAGGGC ACGGGCAGCT TGCCGGTGGT GCAGATGAAC
 2281 TTCAGGGTCA GCTTGCCGTA GGTGGCATCG CCCTCGCCCT CGCCGGACAC GCTGAACTTG
 2341 TGGCCGTTTA CGTCGCCGTC CAGCTCGACC AGGATGGGCA CCACCCCGGT GAACAGCTCC
 2401 TCGCCCTTGC TCACCATGGT GGCGACCGGT AAGCTAGCTT GGGCTGCAGG TCGAAAGGCC
 2461 CGGAGATGAG GAAGAGGAGA ACAGCGCGGC AGACGTGCGC TTTTGAAGCG TGCAGAATGC
 2521 CGGGCCTCCG GAGGACCTTC GGGCGCCCGC CCCGCCCCTG AGCCCGCCCC TGAGCCCGCC
 2581 CCCGGACCCA CCCCTTCCCA GCCTCTGAGC CCAGAAAGCG AAGGAGCAAA GCTGCTATTG
 2641 GCCGCTGCCC CAAAGGCCTA CCCGCTTCCA TTGCTCAGCG GTGCTGTCCA TCTGCACGAG
 2701 ACTAGTGAGA CGTGCTACTT CCATTTGTCA CGTCCTGCAC GACGCGAGCT GCGGGGCGGG
 2761 GGGGAACTTC CTGACTAGGG GAGGAGTAGA AGGTGGCGCG AAGGGGCCAC CAAAGAACGG
 2821 AGCCGGTTGG CGCCTACCGG CCGGTGGATG TGGAATGTGT GCGAGGCCAG AGGCCACTTG
 2881 TGTAGCGCCA AGTGCCCAGC GGGGCTGCTA AAGCGCATGC TCCAGACTGC CTTGGGAAAA
 2941 GCGCCTCCCC TACCCGGTAG AATTCGAACG CTGACGTCAT CAACCCGCTC CAAGGAATCG
 3001 CGGGCCCAGT GTCACTAGGC GGGAACACCC AGCGCGCGTG CGCCCTGGCA GGAAGATGGC
 3061 TGTGAGGGAC AGGGGAGTGG CGCCCTGCAA TATTTGCATG TCGCTATGTG TTCTGGGAAA
 3121 TCACCATAAA CGTGAAATGT CTTTGGATTT GGGAATCTTA TAAGTTCTGT ATGAGACCAC
 3181 AGATCTAAGC TTATCGATAC CGTCGACCTC GAGGGGGGGC CCGGTACCCA GCTTTTGTTC
 3241 CCTTTAGTGA GGGTTAATTG CGCGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG
 3301 AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC
 3361 CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT
 3421 CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG
 3481 CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT
 3541 TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC
 3601 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA
 3661 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA
 3721 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC
 3781 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC
 3841 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG
 3901 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA
 3961 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC
 4021 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC
 4081 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG
 4141 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA
 4201 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA
 4261 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA
 4321 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT
 4381 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG
 4441 TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT
 4501 AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC
 4561 CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA
 4621 CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA
 4681 GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA
 4741 CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT
 4801 CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC
 4861 GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT
 4921 CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC
 4981 TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG
 5041 CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT
 5101 CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC
 5161 CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG
 5221 CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC
 5281 ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG
 5341 TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT
 5401 TCCGCGCACA TTTCCCCGAA AAGTGCCAC
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