pET-42c(+)

价格:800元

联系方式:I47-825O-882O

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pET42c载体基本信息

出品公司: EMD Biosciences (Novagen)
别名: pET42c, pET 42c
质粒类型: 大肠杆菌蛋白表达
表达水平:
克隆方法: 多克隆位点,限制性内切酶
载体大小: 5931 bp
5' 测序引物: T7或pGEX-5‘
5' 测序引物序列:   T7: 5'-TAATACGACTCACTATAGGG-3'; pGEX-5': 5'-GGGCTGGCAAGCCACGTTTGGTG-3'
载体标签: N-GST, N-His, N-S, C-His, N-Thrombin, N-Factor Xa
载体抗性: Kanamycin
表达宿主菌: BL21(DE3) Rosetta2(DE3)C43(DE3)
备注:
Encodes GST fusion tag; Nterm thrombin cleavage site; Nterm Factor Xa cleavage site; a,b,c vary by MCS.
产品目录号: 70563-3
稳定性: 瞬时表达 Transient
组成型: 组成型 Constitutive
病毒/非病毒: 非病毒

pET42c载体质粒图谱和多克隆位点信息

pet42c载体图谱

pet42c多克隆位点

Feature Name Start End  
T7_terminator 120 1
T7_Terminal_primer 69 87
S15 352 308
6xHIS 412 395
GST (variant) 1093 443
T7_transl_en_RBS 1118 1102
lacO 1163 1136
T7_promoter 1181 1163
tet (300 - 563) 1217 1480
pBRrevBam_primer 1288 1269
lacI 1563 2654
ROP 3226 3417
pGEX_3_primer 3433 3411
pBR322_origin 4451 3832
KanR2 4557 5372
ORF Start End  
ORF frame 3 1093 143
ORF frame 2 1094 1822
ORF frame 3 1695 2654
ORF frame 3 4557 5372
Enzyme Name Cut
XhoI 174
NotI 182
EagI 182
HindIII 189
SalI 195
SacI 206
PstI 214
AscI 216
StuI 226
EcoRI 235
BamHI 241
EcoRV 251
NcoI 256
AgeI 290
KpnI 297
BglII 300
SacII 391
SpeI 422
MscI 885
NdeI 1092
XbaI 1130
ApaI 2133
HpaI 2428
NruI 4645
SmaI 4862
XmaI 4860

pET42c载体简介

The pET-42 series is designed for cloning and high-level expression of peptide sequences fused with the 220 aa GST•Tag™ protein. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circle map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Therefore, single stranded sequencing should be performed using the T7 terminator primer (cat. no. 69337-3). Vector encoded sequence can be completely removed when cloning into the PshAI site (as shown below) and then cleaving the GST fusion protein with Factor Xa.

pET42c载体序列

LOCUS       pET-42 c(+)    5931 bp     DNA  Circular    SYN
DEFINITION  pET-42 c(+)
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTOAUTHORAD1|Novagen Inc.|
FEATURES             Location/Qualifiers
     source          1..5931
                     /organism="pET-42 c(+)"
                     /mol_type="other DNA"
     terminator      complement(1..120)
                     /label="T7_terminator"
     misc_feature    69..87
                     /label="T7_Terminal_primer"
     CDS             complement(143..1093)
                     /label="ORF frame 3"
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNK
                     KFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDI
                     RYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALD
                     VVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK
                     SDGSTSGSGHHHHHHSAGLVPRGSTAIGMKETAAAKFERQHMDSPDLGTGGGSGIEGR
                     GSMAISGIRILYRPWRACRRAPSTSLRPHSSTTTTTTTTN*"
     misc_feature    complement(308..352)
                     /label="S15"
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNK
                     KFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDI
                     RYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALD
                     VVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK
                     SDGSTSGSGHHHHHHSAGLVPRGSTAIGMKETAAAKFERQHMDSPDLGTGGGSGIEGR
                     GSMAISGIRILYRPWRACRRAPSTSLRPHSSTTTTTTTTN*"
     misc_feature    complement(395..412)
                     /label="6xHIS"
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNK
                     KFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDI
                     RYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALD
                     VVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK
                     SDGSTSGSGHHHHHHSAGLVPRGSTAIGMKETAAAKFERQHMDSPDLGTGGGSGIEGR
                     GSMAISGIRILYRPWRACRRAPSTSLRPHSSTTTTTTTTN*"
     gene            complement(443..1093)
                     /label="GST (variant)"
                     /gene="GST (variant)"
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNK
                     KFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDI
                     RYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALD
                     VVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK
                     SDGSTSGSGHHHHHHSAGLVPRGSTAIGMKETAAAKFERQHMDSPDLGTGGGSGIEGR
                     GSMAISGIRILYRPWRACRRAPSTSLRPHSSTTTTTTTTN*"
     CDS             1094..1822
                     /label="ORF frame 2"
                     /translation="MYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSISILYAGR
                     IVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHA
                     PFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGEC
                     ETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQ
                     TVVADWRCHLQSGPARAVANCRGD*"
     misc_feature    complement(1102..1118)
                     /label="T7_transl_en_RBS"
                     /translation="MYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSISILYAGR
                     IVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHA
                     PFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGEC
                     ETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQ
                     TVVADWRCHLQSGPARAVANCRGD*"
     misc_feature    complement(1136..1163)
                     /label="lacO"
                     /translation="MYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSISILYAGR
                     IVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHA
                     PFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGEC
                     ETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQ
                     TVVADWRCHLQSGPARAVANCRGD*"
     promoter        complement(1163..1181)
                     /label="T7_promoter"
                     /translation="MYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSISILYAGR
                     IVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHA
                     PFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGEC
                     ETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQ
                     TVVADWRCHLQSGPARAVANCRGD*"
     gene            1217..1480
                     /label="tet (300 - 563)"
                     /gene="tet (300 - 563)"
                     /translation="MYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSISILYAGR
                     IVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHA
                     PFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGEC
                     ETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQ
                     TVVADWRCHLQSGPARAVANCRGD*"
     misc_feature    complement(1269..1288)
                     /label="pBRrevBam_primer"
                     /translation="MYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSISILYAGR
                     IVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHA
                     PFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGEC
                     ETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQ
                     TVVADWRCHLQSGPARAVANCRGD*"
     misc_feature    1563..2654
                     /label="lacI"
                     /translation="MYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSISILYAGR
                     IVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHA
                     PFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGEC
                     ETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQ
                     TVVADWRCHLQSGPARAVANCRGD*"
     CDS             1695..2654
                     /label="ORF frame 3"
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                     SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
     misc_feature    3226..3417
                     /label="ROP"
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                     SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
     misc_feature    complement(3411..3433)
                     /label="pGEX_3_primer"
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                     SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
     rep_origin      complement(3832..4451)
                     /label="pBR322_origin"
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                     SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
     gene            4557..5372
                     /label="KanR2"
                     /gene="KanR2"
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                     SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
     CDS             4557..5372
                     /label="ORF frame 3"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
ORIGIN
    1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
   61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
  121 tgtttagcag cctaggtatt aatcaattag tggtggtggt ggtggtggtg gtgctcgagt
  181 gcggccgcaa gcttgtcgac ggagctcgcc tgcaggcgcg ccaaggcctg tacagaattc
  241 ggatccccga tatcgccatg gacccgcgtc cctcaatacc ggagccacca ccggtaccca
  301 gatctgggct gtccatgtgc tggcgttcga atttagcagc agcggtttct ttcataccaa
  361 ttgcagtact accgcgtggc accagacccg cggagtgatg gtgatggtga tgaccagaac
  421 cactagttga accatccgat tttggaggat ggtcgccacc accaaacgtg gcttgccagc
  481 cctgcaaagg ccatgctata tacttgctgg atttcaagta cttatcaatt tgtgggatag
  541 cttcaatacg ttttttaaaa caaactaatt ttgggaacgc atccaggcac attgggtcca
  601 tgtataaaac aacatcaaga gcgtcataca acatgaagtc aggatgggtt acatgatcac
  661 catttaaata tgttttatga cataaacgat cttcgaacat tttcagcatt tcaggtagct
  721 tgctaagaaa atcaactttg agagtttcaa agtctttact atatgcaatt ctcgaaacac
  781 cgtatctaat atccaaaacc gctccttcaa gcattgaaat ctctgcacgc tcttttggac
  841 aaccacccaa catgttgtgc ttgtcagcta tataacgtat gatggccata gactgtgtta
  901 atttaacatc accatcaata taataaggaa gattgggaaa ctccaaaccc aattcaaact
  961 ttttgtttcg ccatttatca ccttcatcgc gctcatacaa atgctcttca tatttttctt
 1021 caagatattc caaaagaagt cgagtgggtt gcacaaggcc cttaattttc caataaccta
 1081 gtatagggga catatgtata tctccttctt aaagttaaac aaaattattt ctagagggga
 1141 attgttatcc gctcacaatt cccctatagt gagtcgtatt aatttcgcgg gatcgagatc
 1201 gatctcgatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt
 1261 tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct
 1321 catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg
 1381 cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact
 1441 actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgagatc ccggacacca
 1501 tcgaatggcg caaaaccttt cgcggtatgg catgatagcg cccggaagag agtcaattca
 1561 gggtggtgaa tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt
 1621 atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa
 1681 aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg
 1741 cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt
 1801 cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt
 1861 cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc
 1921 aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg
 1981 aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca
 2041 acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat
 2101 tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc
 2161 gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg
 2221 aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca
 2281 tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca
 2341 ttaccgagtc cgggctgcgc gttggtgcgg acatctcggt agtgggatac gacgataccg
 2401 aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg
 2461 ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc
 2521 agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg
 2581 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
 2641 aaagcgggca gtgagcgcaa cgcaattaat gtaagttagc tcactcatta ggcaccggga
 2701 tctcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc
 2761 atgactagca tgatcgtgct cctgtcgttg aggacccggc taggctggcg gggttgcctt
 2821 actggttagc agaatgaatc accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa
 2881 acgtctgcga cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg
 2941 gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc aggatgctgc
 3001 tggctaccct gtggaacacc tacatctgta ttaacgaagc gctggcattg accctgagtg
 3061 atttttctct ggtcccgccg catccatacc gccagttgtt taccctcaca acgttccagt
 3121 aaccgggcat gttcatcatc agtaacccgt atcgtgagca tcctctctcg tttcatcggt
 3181 atcattaccc ccatgaacag aaatccccct tacacggagg catcagtgac caaacaggaa
 3241 aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct tctggagaaa
 3301 ctcaacgagc tggacgcgga tgaacaggca gacatctgtg aatcgcttca cgaccacgct
 3361 gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
 3421 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
 3481 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
 3541 agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag
 3601 tgcaccatat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
 3661 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
 3721 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
 3781 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
 3841 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
 3901 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
 3961 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
 4021 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
 4081 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
 4141 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
 4201 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
 4261 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
 4321 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
 4381 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
 4441 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
 4501 tttggtcatg aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga
 4561 gccatattca acgggaaacg tcttgctcta ggccgcgatt aaattccaac atggatgctg
 4621 atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc
 4681 gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg
 4741 ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc
 4801 cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc
 4861 ccgggaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg
 4921 atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta
 4981 acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg
 5041 atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa
 5101 tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg
 5161 ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa
 5221 tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt
 5281 cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc
 5341 agtttcattt gatgctcgat gagtttttct aagaattaat tcatgagcgg atacatattt
 5401 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
 5461 cctgaaattg taaacgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
 5521 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
 5581 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
 5641 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
 5701 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
 5761 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
 5821 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
 5881 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc a

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