pET-23a(+)

价格:800元

联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

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pET23a载体基本信息

出品公司: EMD Biosciences(Novagen)
别名: pET23a, pet 23a
质粒类型: 大肠杆菌蛋白表达
表达水平:
克隆方法: 多克隆位点,限制性内切酶
载体大小: 3666bp
5' 测序引物: T7
5' 测序引物序列:   5'-TAATACGACTCACTATAGGG-3'
载体标签: N-T7, C-His
载体抗性: Ampicillin
克隆菌株: DH5a
表达菌株: BL21系列
备注:
Same as pET21abcd(+) but no lac; a,b,c,d vary by MCS.
产品目录号:  69745-3
稳定性: 稳表达
组成型: 组成型
病毒/非病毒: 非病毒
表达宿主菌: BL21(DE3)BLR(DE3)Rosetta(DE3)OrigamiB(DE3)
pET表达手册: pET表达手册英文版
pET表达手册中文版
pET载体汇总:
pET-3a     pET-11a   pET-12a   pET-14b   pET-15b   pET-16b
pET-17b   pET-19b   pET-20b   pET-21a   pET-22b   pET-23a
pET-24a   pET-25b   pET-26b   pET-27b   pET-28a   pET-29a
pET-30a   pET-31b   pET-32a   pET-33b   pET-34b   pET-35b
pET-37b   pET-39b   pET-40b   pET-41a   pET-42a   pET-43.1a
pET-44a   pET-45b   pET-47b   pET-48b   pET-49b   pET-50b
pET-51b   pET-52b  
pETDuet-1  pACYCDuet-1  pCDFDuet-1 pRSFDuet-1  pColaDuet-1

pET23a载体图谱质粒图谱和多克隆位点信息

pET-23a载体图谱



pET-23a多克隆位点

pET-23a载体特征

pET23a载体简介

pET-23a-d系列载体带有N-T7标签和C-His标签。pET-23a-d系列载体与pET-21a-d系列载体相比,启动子由pet21a系列载体的T7lac启动子,更改为pET-23a-d载体的T7启动子。独特的酶切位点在载体图谱中被标注出来。需要注意的是,这些载体序列是依据载体pBR322的方向来进行编号的,所以T7标签表达区域在载体图谱上是反向的。


pET Vectors 选择指南

pET23a载体序列

LOCUS       pET-23a(+)              3666 bp    DNA     circular     11-SEP-2008
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTOAUTHORAD1|Novagen Inc.|
FEATURES             Location/Qualifiers
     promoter        complement(303..319)
                     /vntifkey="30"
                     /label=T7\promoter
     protein_bind    complement(207..239)
                     /vntifkey="31"
                     /label=T7\tag
     protein_bind    complement(140..157)
                     /vntifkey="31"
                     /label=His\tag
     terminator      complement(26..72)
                     /vntifkey="43"
                     /label=T7\terminator
     rep_origin      complement(1450..1450)
                     /vntifkey="33"
                     /label=ColE1\pBR322\origin
     CDS             complement(2211..3068)
                     /vntifkey="4"
                     /label=bla\(Ap)\sequence
     rep_origin      complement(3200..3655)
                     /vntifkey="33"
                     /label=f1\origin
BASE COUNT      919 a       936 c       926 g       885 t
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
      181 cgacggagct cgaattcgga tccgcgaccc atttgctgtc caccagtcat gctagccata
      241 tgtatatctc cttcttaaag ttaaacaaaa ttatttctag agggaaaccg ttgtggtctc
      301 cctatagtga gtcgtattaa tttcgcggga tcgagatctc gggcagcgtt gggtcctggc
      361 cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc
      421 ttactggtta gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca
      481 aaacgtctgc gacctgagca acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc
      541 tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg gatctgcatc gcaggatgct
      601 gctggctacc ctgtggaaca cctacatctg tattaacgaa gcgctggcat tgaccctgag
      661 tgatttttct ctggtcccgc cgcatccata ccgccagttg tttaccctca caacgttcca
      721 gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag catcctctct cgtttcatcg
      781 gtatcattac ccccatgaac agaaatcccc cttacacgga ggcatcagtg accaaacagg
      841 aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca gacattaacg cttctggaga
      901 aactcaacga gctggacgcg gatgaacagg cagacatctg tgaatcgctt cacgaccacg
      961 ctgatgagct ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac
     1021 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     1081 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac
     1141 gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag
     1201 agtgcaccat atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     1261 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     1321 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     1381 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     1441 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     1501 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     1561 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     1621 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     1681 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     1741 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     1801 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     1861 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     1921 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     1981 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     2041 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     2101 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     2161 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     2221 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     2281 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     2341 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     2401 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     2461 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     2521 gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     2581 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     2641 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     2701 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     2761 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     2821 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     2881 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     2941 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     3001 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     3061 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     3121 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     3181 ccccgaaaag tgccacctga aattgtaaac gttaatattt tgttaaaatt cgcgttaaat
     3241 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     3301 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     3361 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     3421 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     3481 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
     3541 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     3601 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat
     3661 tcgcca
//

pET23a载体图谱质粒图谱pdf版和相关资料下载

pET23a载体质粒应用举例

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