pGR106

LOCUS       pGR106               10451 bp DNA     circular SYN 13-JUL-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10451)
  AUTHORS   Biofeng
  TITLE     Direct Submission
  JOURNAL   Exported Jul 13, 2023 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..10451
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    540..562
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     promoter        659..1004
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     regulatory      1710..1719
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     terminator      7679..7931
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(7938..7954)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(8121..8139)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8160..8176)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8184..8200
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8208..8238)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8253..8274
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    8513..8537
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(8628..9216)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9317..10132)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYGLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      join(10423..10451,1..407)
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
       61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
      121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
      181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
      241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
      301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
      361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgccag cgcctttgcc
      421 cacaacccgg cggccggccg caacagatcg ttttataaat tttttttttt gaaaaagaaa
      481 aagcccgaaa ggcggcaacc tctcgggctt ctggatttcc gatccccgga attagatctt
      541 ggcaggatat attgtggtgt aacgttctcg catgcctgca ggtcaacatg gtggagcacg
      601 acacgcttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa agggcaattg
      661 agacttttca acaaagggta atatccggaa acctcctcgg attccattgc ccagctatct
      721 gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc catcattgcg
      781 ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa gatggacccc
      841 cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg
      901 attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag
      961 acccttcctc tatataagga agttcatttc atttggagag gagaaaacta aaccatacac
     1021 caccaacaca accaaaccca ccacgcccaa ttgttacaca cccgcttgaa aaagaaagtt
     1081 taacaaatgg ccaaggtgcg cgaggtttac caatctttta cagactccac cacaaaaact
     1141 ctcatccaag atgaggctta tagaaacatt cgccccatca tggaaaaaca caaactagct
     1201 aacccttacg ctcaaacggt tgaagcggct aatgatctag aggggttcgg catagccacc
     1261 aatccctata gcattgaatt gcatacacat gcagccgcta agaccataga gaataaactt
     1321 ctagaggtgc ttggttccat cctaccacaa gaacctgtta catttatgtt tcttaaaccc
     1381 agaaagctaa actacatgag aagaaacccg cggatcaagg acattttcca aaatgttgcc
     1441 attgaaccaa gagacgtagc caggtacccc aaggaaacaa taattgacaa actcacagag
     1501 atcacaacgg aaacagcata cattagtgac actctgcact tcttggatcc gagctacata
     1561 gtggagacat tccaaaactg cccaaaattg caaacattgt atgcgacctt agttctcccc
     1621 gttgaggcag cctttaaaat ggaaagcact cacccgaaca tatacagcct caaatacttc
     1681 ggagatggtt tccagtatat accaggcaac catggtggcg gggcatacca tcatgaattc
     1741 gctcatctac aatggctcaa agtgggaaag atcaagtgga gggaccccaa ggatagcttt
     1801 ctcggacatc tcaattacac gactgagcag gttgagatgc acacagtgac agtacagttg
     1861 caggaatcgt tcgcggcaaa ccacttgtac tgcatcagga gaggagactt gctcacaccg
     1921 gaggtgcgca ctttcggcca acctgacagg tacgtgattc caccacagat cttcctccca
     1981 aaagttcaca actgcaagaa gccgattctc aagaaaacta tgatgcagct cttcttgtat
     2041 gttaggacag tcaaggtcgc aaaaaattgt gacatttttg ccaaagtcag acaattaatt
     2101 aaatcatctg acttggacaa atactctgct gtggaactgg tttacttagt aagctacatg
     2161 gagttccttg ccgatttaca agctaccacc tgcttctcag acacactttc tggtggcttg
     2221 ctaacaaaga cccttgcacc ggtgagggct tggatacaag agaaaaagat gcagctgttt
     2281 ggtcttgagg actacgcgaa gttagtcaaa gcagttgatt tccacccggt ggatttttct
     2341 ttcaaagtgg aaacttggga cttcagattc caccccttgc aagcgtggaa agccttccga
     2401 ccaagggaag tgtcggatgt agaggaaatg gaaagtttgt tctcagatgg ggacctgctt
     2461 gattgcttca caagaatgcc agcttatgcg gtaaacgcag aggaagattt agctgcaatc
     2521 aggaaaacgc ccgagatgga tgtcggtcaa gaagttaaag agcctgcagg agacagaaat
     2581 caatactcaa accctgcaga aactttcctc aacaagctcc acaggaaaca cagtagggag
     2641 gtgaaacacc aggccgcaaa gaaagctaaa cgcctagctg aaatccagga gtcaatgaga
     2701 gctgaaggtg atgccgaacc aaatgaaata agcgggacga tgggggcaat acccagcaac
     2761 gccgaacttc ctggcacgaa tgatgccaga caagaactca cactcccaac cactaaacct
     2821 gtccctgcaa ggtgggaaga tgcttcattc acagattcta gtgtggaaga ggagcaggtt
     2881 aaactccttg gaaaagaaac cgttgaaaca gcgacgcaac aagtcatcga aggacttcct
     2941 tggaaacact ggattcctca attaaatgct gttggattca aggcgctgga aattcagagg
     3001 gataggagtg gaacaatgat catgcccatc acagaaatgg tctccgggct ggaaaaagag
     3061 gacttccctg aaggaactcc aaaagagttg gcacgagaat tgttcgctat gaacagaagc
     3121 cctgccacca tccctttgga cctgcttaga gccagagact acggcagtga tgtaaagaac
     3181 aagagaattg gtgccatcac aaagacacag gcaacgagtt ggggcgaata cttgacagga
     3241 aagatagaaa gcttaactga gaggaaagtt gcgacttgtg tcattcatgg agctggaggt
     3301 tctggaaaaa gtcatgccat ccagaaggca ttgagagaaa ttggcaaggg ctcggacatc
     3361 actgtagtcc tgccgaccaa tgaactgcgg ctagattgga gtaagaaagt gcctaacact
     3421 gagccctata tgttcaagac ctctgaaaag gcgttaattg ggggaacagg cagcatagtc
     3481 atctttgacg attactcaaa acttcctccc ggttacatag aagccttagt ctgtttctac
     3541 tctaaaatca agctaatcat tctaacagga gatagcagac aaagcgtcta ccatgaaact
     3601 gctgaggacg cctccatcag gcatttggga ccagcaacag agtacttctc aaaatactgc
     3661 cgatactatc tcaatgccac acaccgcaac aagaaagatc ttgcgaacat gcttggtgtc
     3721 tacagtgaga gaacgggagt caccgaaatc agcatgagcg ccgagttctt agaaggaatc
     3781 ccaactttgg taccctcgga tgagaagaga aagctgtaca tgggcaccgg gaggaatgac
     3841 acgttcacat acgctggatg ccaggggcta actaagccga aggtacaaat agtgttggac
     3901 cacaacaccc aagtgtgtag cgcgaatgtg atgtacacgg cactttctag agccaccgat
     3961 aggattcact tcgtgaacac aagtgcaaat tcctctgcct tctgggaaaa gttggacagc
     4021 accccttacc tcaagacttt cctatcagtg gtgagagaac aagcactcag ggagtacgag
     4081 ccggcagagg cagagccaat tcaagagcct gagccccaga cacacatgtg tgtcgagaat
     4141 gaggagtccg tgctagaaga gtacaaagag gaactcttgg aaaagtttga cagagagatc
     4201 cactctgaat cccatggtca ttcaaactgt gtccaaactg aagacacaac cattcagttg
     4261 ttttcgcatc aacaagcaaa agatgagacc ctcctctggg cgactataga tgcgcggctc
     4321 aagaccagca atcaagaaac aaacttccga gaattcctga gcaagaagga cattggggac
     4381 gttctgtttt taaactacca aaaagctatg ggtttaccca aagagcgtat tcctttttcc
     4441 caagaggtct gggaagcttg tgcccacgaa gtacaaagca agtacctcag caagtcaaag
     4501 tgcaacttga tcaatgggac tgtgagacag agcccagact tcgatgaaaa taagattatg
     4561 gtattcctca agtcgcagtg ggtcacaaag gtggaaaaac taggtctacc caagattaag
     4621 ccaggtcaaa ccatagcagc cttttaccag cagactgtga tgctttttgg aactatggct
     4681 aggtacatgc gatggttcag acaggctttc cagccaaaag aagtcttcat aaactgtgag
     4741 accacgccag atgacatgtc tgcatgggcc ttgaacaact ggaatttcag cagacctagc
     4801 ttggctaatg actacacagc tttcgaccag tctcaggatg gagccatgtt gcaatttgag
     4861 gtgctcaaag ccaaacacca ctgcatacca gaggaaatca ttcaggcata catagatatt
     4921 aagactaatg cacagatttt cctaggcacg ttatcaatta tgcgcctgac tggtgaaggt
     4981 cccacttttg atgcaaacac tgagtgcaac atagcttaca cccatacaaa gtttgacatc
     5041 ccagccggaa ctgctcaagt ttatgcagga gacgactccg cactggactg tgttccagaa
     5101 gtgaagcata gtttccacag gcttgaggac aaattactcc taaagtcaaa gcctgtaatc
     5161 acgcagcaaa agaagggcag ttggcctgag ttttgtggtt ggctgatcac accaaaaggg
     5221 gtgatgaaag acccaattaa gctccatgtt agcttaaaat tggctgaagc taagggtgaa
     5281 ctcaagaaat gtcaagattc ctatgaaatt gatctgagtt atgcctatga ccacaaggac
     5341 tctctgcatg acttgttcga tgagaaacag tgtcaggcac acacactcac ttgcagaaca
     5401 ctaatcaagt cagggagagg cactgtctca ctttcccgcc tcagaaactt tctttaaccg
     5461 ttaagttacc ttagagattt gaataagatg gatattctca tcagtagttt gaaaagttta
     5521 ggttattcta ggacttccaa atctttagat tcaggacctt tggtagtaca tgcagtagcc
     5581 ggagccggta agtccacagc cctaaggaag ttgatcctca gacacccaac attcaccgtg
     5641 catacactcg gtgtccctga caaggtgagt atcagaacta gaggcataca gaagccagga
     5701 cctattcctg agggcaactt cgcaatcctc gatgagtata ctttggacaa caccacaagg
     5761 aactctaacc aggcactttt tgctgaccct tatcaggcac cggagtttag cctagagccc
     5821 cacttctact tggaaacatc atttcgagtt ccgaggaaag tggcagattt gatagctggc
     5881 tgtggcttcg atttcgagac caactcaccg gaagaagggc acttagagat cactggcata
     5941 ttcaaagggc ccctactcgg aaaggtgata gccattgatg aggagtctga gacaacactg
     6001 tccaggcatg gtgttgagtt tgttaagccc tgccaagtga cgggacttga gttcaaagta
     6061 gtcactattg tgtctgccgc accaatagag gaaattggcc agtccacagc tttctacaac
     6121 gctatcacca ggtcaaaggg attgacatat gtccgcgcag ggccataggc tgaccgctcc
     6181 ggtcaattct gaaaaagtgt acatagtatt aggtctatca tttgctttag tttcaattac
     6241 ctttctgctt tctagaaata gcttacccca cgtcggtgac aacattcaca gcttgccaca
     6301 cggaggagct tacagagacg gcaccaaagc aatcttgtac aactccccaa atctagggtc
     6361 acgagtgagt ctacacaacg gaaagaacgc agcatttgct gccgttttgc tactgacttt
     6421 gctgatctat ggaagtaaat acatatctca acgcaatcat acttgtgctt gtggtaacaa
     6481 tcatagcagt cattagcact tccttagtga ggactgaacc ttgtgtcatc aagattactg
     6541 gggaatcaat cacagtgttg gcttgcaaac tagatgcaga aaccataagg gccattgccg
     6601 atctcaagcc actctccgtt gaacggttaa gtttccattg atactcgaaa gaggtcagca
     6661 ccagctagca tcgattggcg cgcctggagc ggccgcgtcg accgccgata agcttgatag
     6721 ggccattgcc gatctcaagc cactctccgt tgaacggtta agtttccatt gatactcgaa
     6781 agatgtcagc accagctagc acaacacagc ccatagggtc aactacctca actaccacaa
     6841 aaactgcagg cgcaactcct gccacagctt caggcctgtt caccatcccg gatggggatt
     6901 tctttagtac agcccgtgcc atagtagcca gcaatgctgt cgcaacaaat gaggacctca
     6961 gcaagattga ggctatttgg aaggacatga aggtgcccac agacactatg gcacaggctg
     7021 cttgggactt agtcagacac tgtgctgatg taggatcatc cgctcaaaca gaaatgatag
     7081 atacaggtcc ctattccaac ggcatcagca gagctagact ggcagcagca attaaagagg
     7141 tgtgcacact taggcaattt tgcatgaagt atgctccagt ggtatggaac tggatgttaa
     7201 ctaacaacag tccacctgct aactggcaag cacaaggttt caagcctgag cacaaattcg
     7261 ctgcattcga cttcttcaat ggagtcacca acccagctgc catcatgccc aaagaggggc
     7321 tcatccggcc accgtctgaa gctgaaatga atgctgccca aactgctgcc tttgtgaaga
     7381 ttacaaaggc cagggcacaa tccaacgact ttgccagcct agatgcagct gtcactcgag
     7441 gtcgtatcac tggaacaaca accgctgagg ctgttgtcac tctaccacca ccataactac
     7501 gtctacataa ccgacgccta ccccagtttc atagtatttt ctggtttgat tgtatgaata
     7561 atataaataa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     7621 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaactagtg ggtaccgcga atttccccga
     7681 tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat
     7741 gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat
     7801 gacgttattt atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc
     7861 gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat
     7921 gttactagat cgaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     7981 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     8041 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg
     8101 atgagctcca gcttttgttc cctttagtga gggttaattc cgagcttggc gtaatcatgg
     8161 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     8221 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     8281 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     8341 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     8401 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     8461 atacggttat ccacagaatc aggggataac gcaggaaaga acatgaaggc cttgacagga
     8521 tatattggcg ggtaaactaa gtcgctgtat gtgtttgttt gagatctcat gtgagcaaaa
     8581 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     8641 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     8701 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     8761 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     8821 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     8881 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     8941 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     9001 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     9061 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     9121 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     9181 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     9241 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat ggttacaacc
     9301 aattaaccaa ttctgattag aaaaactcat cgagcatcaa atgaaactgc aatttattca
     9361 tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact
     9421 caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc
     9481 caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat
     9541 caccatgagt gacgactgaa tccggtgaga atggcaaaag tttatgcatt tctttccaga
     9601 cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt
     9661 tattcattcg tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta aaaggacaat
     9721 tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt
     9781 cacctgaatc aggatattct tctaatacct ggaatgctgt tttccctggg atcgcagtgg
     9841 tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa
     9901 attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt
     9961 tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcca tagattgtcg
    10021 cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt
    10081 tggaatttaa tcgcggcctg gagcaagacg tttcccgttg aatatggctc ataacacccc
    10141 ttgtattact gtttatgtaa gcagacagtt ttattgttca tgatgatata tttttatctt
    10201 gtgcaatgta acatcagaga ttttgagaca caacgtggct ttgttgaata aatcgaactt
    10261 ttgctgagtt gaaggatcag atcacgcatc ttcccgacaa cgcagaccgt tccgtggcaa
    10321 agcaaaagtt caaaatcacc aactggtcca cctacaacaa agctctcatc aaccgtggct
    10381 ccctcacttt ctggctggat gatggggcga ttcaggcgat ccccatccaa cagcccgccg
    10441 tcgagcgggc t
//