| 质粒名称: | pT7-NSP4SA11 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 89171 |
| 存储实验室: | Takeshi Kobayashi |
|
载体骨架基本信息 |
|
| 载体名称: | p3E5 |
| 载体出品公司: | -- |
| 空载体大小: | 3074 bp |
| 完整质粒大小: | 3825 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Rotavirus SA11 NSP4 |
| 物种来源: | Rotavirus SA11 |
| 插入大小: | 751 bp |
| 基因突变: | -- |
| GenBank编号 | LC178573 |
| 启动子: | T7 promotor |
| 标签/融合蛋白: | -- |
| 克隆方法: | Gibson克隆 |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | T7 forward CTGTGGATAACCGTATTACCG |
| 3’测序引物 | T7 terminal GCTAGTTATTGCTCAGCGG |
|
文献引用方法 |
|
| 材料和方法部分: | pT7-NSP4SA11 was a gift from Takeshi Kobayashi (Addgene plasmid # 89171 ; http://n2t.net/addgene:89171 ; RRID:Addgene_89171) |
| 参考文献部分: | Entirely plasmid-based reverse genetics system for rotaviruses. Kanai Y, Komoto S, Kawagishi T, Nouda R, Nagasawa N, Onishi M, Matsuura Y, Taniguchi K, Kobayashi T. Proc Natl Acad Sci U S A. 2017 Jan 30. pii: 201618424. doi: 10.1073/pnas.1618424114. 10.1073/pnas.1618424114 PubMed 28137864 |
买家导航
pT7NSP4SA11载体基本信息
pT7NSP4SA11载体图谱质粒图谱和多克隆位点信息
pT7NSP4SA11载体简介
pT7NSP4SA11载体序列
LOCUS pT7-NSP4SA11 3825 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Express Rotavirus SA11 NSP4 in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pT7-NSP4SA11
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3825)
AUTHORS Kanai Y, Komoto S, Kawagishi T, Nouda R, Nagasawa N, Onishi M,
Matsuura Y, Taniguchi K, Kobayashi T
TITLE Entirely plasmid-based reverse genetics system for rotaviruses.
JOURNAL Proc Natl Acad Sci U S A. 2017 Jan 30. pii: 201618424. doi:
10.1073/pnas.1618424114.
PUBMED 28137864
REFERENCE 2 (bases 1 to 3825)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..3825
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(285..304)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind complement(514..533)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 633..655
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(693..711)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 779..883
/gene="bla"
/label=AmpR promoter
CDS 884..1744
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(1102..1121)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 1915..2503
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 2404..2423
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 2657..2674
/label=L4440
/note="L4440 vector, forward primer"
promoter 2759..2777
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(3673..3691)
/label=T7 Term
/note="T7 terminator, reverse primer"
terminator 3687..3734
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
promoter complement(3802..3820)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(3803..3820)
/label=SP6
/note="SP6 promoter, forward primer"
ORIGIN
1 gtgtatgata cataaggtta tgtattaatt gtagccgcgt tctaacgaca atatgtacaa
61 gcctaattgt gtagcatctg gcttactgaa gcagacccta tcatctctct cgtaaactgc
121 cgtcagagtc ggtttggttg gacgaacctt ctgagtttct ggtaacgccg tcccgcaccc
181 ggaaatggtc agcgaaccaa tcagcagggt catcgctagc cagatcctct acgccggacg
241 catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat
301 caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg
361 tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc accattcctt
421 gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcttaat gcaggagtcg
481 cataagggag agcgtcgaat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
541 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
601 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
661 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
721 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
781 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
841 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
901 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
961 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
1021 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
1081 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
1141 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
1201 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
1261 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
1321 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
1381 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
1441 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
1501 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
1561 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
1621 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
1681 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
1741 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
1801 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
1861 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
1921 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
1981 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
2041 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
2101 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
2161 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
2221 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
2281 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
2341 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
2401 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
2461 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
2521 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
2581 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
2641 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
2701 accgcctctc cccgcgcgtt ggccgattca ttaatgcagg gggatatcga tcccgggtta
2761 atacgactca ctataggctt ttaaaagttc tgttccgaga gagcgcgtgc ggaaagatgg
2821 aaaagcttac cgacctcaat tatacattga gtgtaatcac tctaatgaac aatacattgc
2881 acacaatact tgaggatcca ggaatggcgt attttcctta tatagcatct gtcttaacag
2941 ttttgtttgc gctacataaa gcatccattc caacaatgaa aattgcattg aaaacgtcaa
3001 aatgttcata taaagtggtg aaatattgta ttgtaacaat ttttaatacg ttgttaaaat
3061 tggcaggtta taaagagcag ataactacta aagatgagat agaaaagcaa atggacagag
3121 tagtcaaaga aatgagacgc cagctagaaa tgattgacaa attgactaca cgtgaaattg
3181 aacaagtaga gttgcttaaa cgcatttacg ataaattgac ggtgcaaacg acaggtgaaa
3241 tagatatgac aaaagagatc aatcaaaaaa acgtgagaac gctagaagaa tgggaaagtg
3301 gaaaaaatcc ttatgaacca agagaagtga ctgcagcaat gtaagaggtt gagctgccgt
3361 cgactgtcct cggaagcggc ggagttcttt acagtaagca ccatcggacc tgatggctga
3421 ctgagaagcc acagtcagcc atatcgcgtg tggctcaagc cttaatcccg tttaaccaat
3481 ccggtcagca ccggacgtta atggaaggaa cggtcttaat gtgaccgggt cggcatggca
3541 tctccacctc ctcgcggtcc gacctgggca tccgaaggag gacgtcgtcc actcggatgg
3601 ctaagggaga gctcaaaaaa aaggatccgg ctgctaacaa agcccgaaag gaagctgagt
3661 tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct
3721 tgaggggttt tttgctgaaa ggaggaacta tatccggatc gagatcctct agagtcgacc
3781 tgcaggcatg caagcttgta ttctatagtg tcacctaaat cgtat
//
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