| 质粒名称: | pKM208 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 13077 |
| 存储实验室: | Kenan Murphy |
|
载体骨架基本信息 |
|
| 载体名称: | pKM201 |
| 载体出品公司: | Kenan Murphy |
| 空载体大小: | 7200 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 载体抗性: | 氨苄霉素100 μg/mL |
| 生长温度: | 30 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | lacI repressor |
| 物种来源: | E. coli |
| 插入大小: | 1400 bp |
| 基因突变: | J01636 |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | NotI (未破坏) |
| 3’克隆位点: | NotI (未破坏) |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pKM208 was a gift from Kenan Murphy (Addgene plasmid # 13077 ; http://n2t.net/addgene:13077 ; RRID:Addgene_13077) |
| 参考文献部分: | Lambda Red-mediated recombinogenic engineering of enterohemorrhagic and enteropathogenic E. coli. Murphy KC, Campellone KG. BMC Mol Biol. 2003 Dec 13. 4():11. 10.1186/1471-2199-4-11 PubMed 14672541 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pKM208 8721 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pKM208
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8721)
AUTHORS Murphy KC, Campellone KG
TITLE Lambda Red-mediated recombinogenic engineering of enterohemorrhagic
and enteropathogenic E. coli.
JOURNAL BMC Mol Biol. 2003 Dec 13. 4():11.
PUBMED 14672541
REFERENCE 2 (bases 1 to 8721)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8721
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 17..33
/label=pBluescriptKS
/note="For pBluescript vector"
primer_bind 18..34
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
promoter 115..143
/label=tac promoter
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
protein_bind 151..167
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS 200..616
/codon_start=1
/gene="gam"
/product="inhibitor of the host RecBCD nuclease in the
lambda Red system"
/label=Gam
/note="protects the double-stranded DNA substrate for
recombination"
/translation="MDINTETEIKQKHSLTPFPVFLISPAFRGRYFHSYFRSSAMNAYY
IQDRLEAQSWARHYQQLAREEKEAELADDMEKGLPQHLFESLCIDHLQRHGASKKSITR
AFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
CDS 622..1407
/codon_start=1
/gene="bet"
/product="single-stranded DNA binding recombinase in the
lambda Red system"
/label=Beta
/note="mediates pairing of a single-stranded DNA with a
complementary target"
/translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQFI
ALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNESC
TCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCARLA
FGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLP
LCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA"
CDS 1404..2084
/codon_start=1
/gene="exo"
/product="5' to 3' double-stranded DNA exonuclease in the
lambda Red system"
/label=Exo
/note="creates 3' single-stranded DNA tails"
/translation="MTPDIILQRTGIDVRAVEQGEDAWHKLRLGVITASEVHNVIAKPR
SGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTESP
IIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQYSMW
VTRKNAWYFANYDPRMKREGLHYVVIERDEKYMASFDEIVPEFIEKMDEALAEIGFVFG
EQWR"
primer_bind complement(2118..2134)
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2118..2134)
/label=pBluescriptSK
/note="For pBluescript vector"
promoter 2152..2229
/gene="lacI (mutant)"
/label=lacIq promoter
/note="In the lacIq allele, a single base change in the
promoter boosts expression of the lacI gene about 10-fold."
CDS 2230..3312
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
primer_bind complement(2249..2268)
/label=LacI-R
/note="LacI, reverse primer"
promoter 3361..3391
/label=lac UV5 promoter
/note="E. coli lac promoter with an ""up"" mutation"
protein_bind 3399..3415
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 3423..3439
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3455..3471)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3608..3628)
/label=T3
/note="T3 promoter, forward primer"
promoter complement(3608..3626)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(3647..3663)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 3671..3687
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3695..3725)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3740..3761
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(3878..3895)
/label=L4440
/note="L4440 vector, forward primer"
CDS complement(5233..6183)
/codon_start=1
/gene="rep101 encoding an A56V mutation"
/product="temperature-sensitive version of the RepA protein
needed for replication with the pSC101 origin (Armstrong et
al., 1984)"
/label=Rep101(Ts)
/translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
rep_origin 6231..6453
/label=pSC101 ori
/note="low-copy replication origin that requires the Rep101
protein"
CDS complement(7081..7941)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7704..7723
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7942..8046)
/gene="bla"
/label=AmpR promoter
rep_origin complement(8072..8527)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(8209..8230)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 8421..8440
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 8669..8685
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 8695..8714
/label=T7
/note="T7 promoter, forward primer"
promoter 8695..8713
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
1 ggtaccgggc cccccctcga ggtcgacggt atcgataagc ttgatatcga attcctgcag
61 cccgggggat ctccgacatc ataacggttc tggcaaatat tctgaaatga gctgttgaca
121 attaatcatc ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
181 acagtcgacg cttataaaaa tggatattaa tactgaaact gagatcaagc aaaagcattc
241 actaaccccc tttcctgttt tcctaatcag cccggcattt cgcgggcgat attttcacag
301 ctatttcagg agttcagcca tgaacgctta ttacattcag gatcgtcttg aggctcagag
361 ctgggcgcgt cactaccagc agctcgcccg tgaagagaaa gaggcagaac tggcagacga
421 catggaaaaa ggcctgcccc agcacctgtt tgaatcgcta tgcatcgatc atttgcaacg
481 ccacggggcc agcaaaaaat ccattacccg tgcgtttgat gacgatgttg agtttcagga
541 gcgcatggca gaacacatcc ggtacatggt tgaaaccatt gctcaccacc aggttgatat
601 tgattcagag gtataaaacg aatgagtact gcactcgcaa cgctggctgg gaagctggct
661 gaacgtgtcg gcatggattc tgtcgaccca caggaactga tcaccactct tcgccagacg
721 gcatttaaag gtgatgccag cgatgcgcag ttcatcgcat tactgatcgt tgccaaccag
781 tacggcctta atccgtggac gaaagaaatt tacgcctttc ctgataagca gaatggcatc
841 gttccggtgg tgggcgttga tggctggtcc cgcatcatca atgaaaacca gcagtttgat
901 ggcatggact ttgagcagga caatgaatcc tgtacatgcc ggatttaccg caaggaccgt
961 aatcatccga tctgcgttac cgaatggatg gatgaatgcc gccgcgaacc attcaaaact
1021 cgcgaaggca gagaaatcac ggggccgtgg cagtcgcatc ccaaacggat gttacgtcat
1081 aaagccatga ttcagtgtgc ccgtctggcc ttcggatttg ctggtatcta tgacaaggat
1141 gaagccgagc gcattgtcga aaatactgca tacactgcag aacgtcagcc ggaacgcgac
1201 atcactccgg ttaacgatga aaccatgcag gagattaaca ctctgctgat cgccctggat
1261 aaaacatggg atgacgactt attgccgctc tgttcccaga tatttcgccg cgacattcgt
1321 gcatcgtcag aactgacaca ggccgaagca gtaaaagctc ttggattcct gaaacagaaa
1381 gccgcagagc agaaggtggc agcatgacac cggacattat cctgcagcgt accgggatcg
1441 atgtgagagc tgtcgaacag ggggaagatg cgtggcacaa attacggctc ggcgtcatca
1501 ccgcttcaga agttcacaac gtgatagcaa aaccccgctc cggaaagaag tggcctgaca
1561 tgaaaatgtc ctacttccac accctgcttg ctgaggtttg caccggtgtg gctccggaag
1621 ttaacgctaa agcactggcc tggggaaaac agtacgagaa cgacgccaga accctgtttg
1681 aattcacttc cggcgtgaat gttactgaat ccccgatcat ctatcgcgac gaaagtatgc
1741 gtaccgcctg ctctcccgat ggtttatgca gtgacggcaa cggccttgaa ctgaaatgcc
1801 cgtttacctc ccgggatttc atgaagttcc ggctcggtgg tttcgaggcc ataaagtcag
1861 cttacatggc ccaggtgcag tacagcatgt gggtgacgcg aaaaaatgcc tggtactttg
1921 ccaactatga cccgcgtatg aagcgtgaag gcctgcatta tgtcgtgatt gagcgggatg
1981 aaaagtacat ggcgagtttt gacgagatcg tgccggagtt catcgaaaaa atggacgagg
2041 cactggctga aattggtttt gtatttgggg agcaatggcg atgacgcatc ctcacgataa
2101 tatccgggta ggcgcaagat ccactagttc tagagcggcc gctaattcgg ggacaccatc
2161 gaatggtgca aaacctttcg cggtatggca tgatagcgcc cggaagagag tcaattcagg
2221 gtggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat
2281 cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa
2341 gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg
2401 ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg
2461 caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg
2521 atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa
2581 cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa
2641 gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac
2701 agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg
2761 ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt
2821 ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa
2881 ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc
2941 gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt
3001 accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa
3061 gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg
3121 caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag
3181 ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc
3241 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
3301 agcgggcagt gagcgcaacg caattaatgt aagttagctc actcattagg caccccaggc
3361 tttacacttt atgcttccgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
3421 cacaggaaac agctatgacc atgattacgg attcactggc cgtcgtttta caacgtcgtg
3481 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
3541 gctggcgtaa tagcgaagag gcccgcaccg agcggccgcc accgcggtgg agctccagct
3601 tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc
3661 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
3721 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
3781 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
3841 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
3901 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
3961 cagaatcagg ggataacgca ggaaagaaca tgtgagatcc ccacagcaag atgcaacttt
4021 cgccagatac gacggcgctc tttgccgtgc tttctgactt tccattcgcc ttcaccaaag
4081 accttcagcc cggtggaatc aatcaccagg tgtgcgattt caccccgggt ggacgttttg
4141 aaactgacat taaccgactt tgcccgctta ctgacactgg tgtaatccgg gcagcgcaac
4201 ggaacgttca tcagggcaaa aatggaatca ataaaaccct gcgcagcccg cagggtcagc
4261 cggaatacgc gtttaatcac cagaacggtg gtgatggcga gatcagaata gcgctggggc
4321 cttcctcgtg atgaaggtgt tgccgactca taccaggcct gaatcgcctc atcatccagc
4381 cagaaagtga gggagccacg gttgatgaga gctttgttgt aggtggacca gttggtgatt
4441 ctgaactttt gctttgccac ggaatggtct gtgttgtcgg gaggatgcgt gatctgatcc
4501 ttcaactcag caaaagttcg atttattcaa caaagccact ttcagtatgg atttgggtgt
4561 tacggggggg ggttggtgct taaaatcgcg ctgaggattt ctgatggtgt taagcgggcg
4621 gttttgagat gtaaactcgc ccgtttaaca taatggatct tgcgcgcacc gcccgaacac
4681 cactcgccac aaaaaaccgc cggaacgtcc aaaagtacgg gttttgctgc ccgcaaacgg
4741 gctgttctgg tgttgctagt ttgttatcag aatcgcagat ccggcttcag ccggtttgcc
4801 ggctgaaagc gctatttctt ccagaattgc catgattttt tccccacggg aggcgtcact
4861 ggctcccgtg ttgtcggcag ctttgattcg ataagcagca tcgcctgttt caggctgtct
4921 atgtgtgact gttgagctgt aacaagttgt ctcaggtgtt caatttcatg ttctagttgc
4981 tttgttttac tggtttcacc tgttctatta ggtgttacat gctgttcatc tgttacattg
5041 tcgatctgtt catggtgaac agctttaaat gcaccaaaaa ctcgtaaaag ctctgatgta
5101 tctatctttt ttacaccgtt ttcatctgtg catatggaca gttttccctt tgatatgtaa
5161 cggtgaacag ttgttctact tttgtttgtt agtcttgatg cttcactgat agatacaaga
5221 gccataagaa cctcagatcc ttccgtattt agccagtatg ttctctagtg tggttcgttg
5281 tttttgcgtg agccatgaga acgaaccatt gagatcatac ttactttgca tgtcactcaa
5341 aaattttgcc tcaaaactgg tgagctgaat ttttgcagtt aaagcatcgt gtagtgtttt
5401 tcttagtccg ttatgtaggt aggaatctga tgtaatggtt gttggtattt tgtcaccatt
5461 catttttatc tggttgttct caagttcggt tacgagatcc atttgtctat ctagttcaac
5521 ttggaaaatc aacgtatcag tcgggcggcc tcgcttatca accaccaatt tcatattgct
5581 gtaagtgttt aaatctttac ttattggttt caaaacccat tggttaagcc ttttaaactc
5641 atggtagtta ttttcaagca ttaacatgaa cttaaattca tcaaggctaa tctctatatt
5701 tgccttgtga gttttctttt gtgttagttc ttttaataac cactcataaa tcctcataga
5761 gtatttgttt tcaaaagact taacatgttc cagattatat tttatgaatt tttttaactg
5821 gaaaagataa ggcaatatct cttcactaaa aactaattct aatttttcgc ttgagaactt
5881 ggcatagttt gtccactgga aaatctcaaa gcctttaacc aaaggattcc tgatttccac
5941 agttctcgtc atcagctctc tggttgcttt agctaataca ccataagcat tttccctact
6001 gatgttcatc atctgaacgt attggttata agtgaacgat accgtccgtt ctttccttgt
6061 agggttttca atcgtggggt tgagtagtgc cacacagcat aaaattagct tggtttcatg
6121 ctccgttaag tcatagcgac taatcgctag ttcatttgct ttgaaaacaa ctaattcaga
6181 catacatctc aattggtcta ggtgatttta atcactatac caattgagat gggctagtca
6241 atgataatta ctagtccttt tcctttgagt tgtgggtatc tgtaaattct gctagacctt
6301 tgctggaaaa cttgtaaatt ctgctagacc ctctgtaaat tccgctagac ctttgtgtgt
6361 tttttttgtt tatattcaag tggttataat ttatagaata aagaaagaat aaaaaaagat
6421 aaaaagaata gatcccagcc ctgtgtataa ctcactactt tagtcagttc cgcagtatta
6481 caaaaggatg tcgcaaacgc tgtttgctcc tctacaaaac agaccttaaa accctaaagg
6541 cttaagtagc accctcgcaa gctcgggcaa atcgctgaat attccttttg tctccgacca
6601 tcaggcacct gagtcgctgt ctttttcgtg acattcagtt cgctgcgctc acggctctgg
6661 cagtgaatgg gggtaaatgg cactacaggc gccttttatg gattcatgca aggaaactac
6721 ccataataca agaaaagccc gtcacgggct tctcagggcg ttttatggcg ggtctgctat
6781 gtggtgctat ctgacttttt gctgttcagc agttcctgcc ctctgatttt ccagtctgac
6841 cacttcggat tatcccgtga caggtcattc agactggcta atgcacccag taaggcagcg
6901 gtatcatcaa caggcttacc cgtcttactg tcggggatct gacgctcagt ggaacgaaaa
6961 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
7021 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
7081 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
7141 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
7201 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
7261 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
7321 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
7381 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
7441 cagctccggt tcccagcgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
7501 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
7561 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
7621 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
7681 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
7741 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
7801 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
7861 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
7921 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
7981 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
8041 tccgcgcaca tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa
8101 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
8161 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
8221 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
8281 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
8341 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
8401 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
8461 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
8521 ggcgcgtccc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
8581 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
8641 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac
8701 gactcactat agggcgaatt g
//
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