pGL4.29

价格:1250元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Promega
载体名称: pGL4.29 、pGL4.29[luc2P/CRE/Hygro]
质粒类型: 哺乳细胞表达;信号通路报告载体
高拷贝/低拷贝: --
启动子: --
克隆方法: 多克隆位点,限制性内切酶
载体大小: 6084 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素Ampicillin
筛选标记: 潮霉素Hygromycin
备注: pGL4.29载体含有荧光素酶报告基因luc2P和环化腺苷酸cAMP应答元件CRE,用于研究细胞内信号通路。
产品目录号: E8471
稳定性: --
组成型: --
病毒/非病毒: --

载体质粒图谱和多克隆位点信息

pGL4.29载体图谱
pGL4.29载体特征

载体简介

The pGL4.29[luc2P/CRE/Hygro] Vector(a–f) contains a cAMP response element (CRE) that drives the
transcription of the luciferase reporter gene luc2P (Photinus pyralis). luc2P is a synthetically-derived luciferase sequence
with humanized codon optimization that is designed for high expression and reduced anomalous transcription. The luc2P
gene contains hPEST, a protein destabilization sequence. The protein encoded by luc2P responds more quickly than the protein
encoded by the luc2 gene upon induction. The vector backbone contains an ampicillin resistance gene to allow selection
in E. coli and a mammalian selectable marker for hygromycin resistance.

载体序列

LOCUS       pGL4.29[luc2P/CRE/Hygro]	6084 bp 	DNA	SYN
DEFINITION  pGL4.29[luc2P/CRE/Hygro]
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..6084
                     /organism="pGL4.29[luc2P/CRE/Hygro]"
                     /mol_type="other DNA"
     CDS             110..1990
                     /label="ORF frame 2"
                     /translation="MGEQIWPRRPSLDTRGYIMEARLPAWQSGTVGKATMEDAKNIKK
                     GPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAHIEVDITYAEYFEMSVRLAEAM
                     KRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPANDIYNERELLNSMGISQPTV
                     VFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVP
                     ESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVV
                     PFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKST
                     LIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGLTETTSAILITPEGDD
                     KPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKD
                     GWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLP
                     DDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLD
                     ARKIREILIKAKKGGKIAVNSHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARI
                     NV*"
     terminator      complement(2036..2155)
                     /label="SV40_PA_terminator"
                     /translation="MGEQIWPRRPSLDTRGYIMEARLPAWQSGTVGKATMEDAKNIKK
                     GPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAHIEVDITYAEYFEMSVRLAEAM
                     KRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPANDIYNERELLNSMGISQPTV
                     VFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVP
                     ESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVV
                     PFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKST
                     LIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGLTETTSAILITPEGDD
                     KPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKD
                     GWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLP
                     DDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLD
                     ARKIREILIKAKKGGKIAVNSHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARI
                     NV*"
     misc_feature    complement(2050..2069)
                     /label="EBV_rev_primer"
                     /translation="MGEQIWPRRPSLDTRGYIMEARLPAWQSGTVGKATMEDAKNIKK
                     GPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAHIEVDITYAEYFEMSVRLAEAM
                     KRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPANDIYNERELLNSMGISQPTV
                     VFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVP
                     ESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVV
                     PFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKST
                     LIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGLTETTSAILITPEGDD
                     KPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKD
                     GWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLP
                     DDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLD
                     ARKIREILIKAKKGGKIAVNSHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARI
                     NV*"
     misc_feature    complement(2377..2397)
                     /label="pBABE_3_primer"
                     /translation="MGEQIWPRRPSLDTRGYIMEARLPAWQSGTVGKATMEDAKNIKK
                     GPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAHIEVDITYAEYFEMSVRLAEAM
                     KRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPANDIYNERELLNSMGISQPTV
                     VFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVP
                     ESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVV
                     PFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKST
                     LIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGLTETTSAILITPEGDD
                     KPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKD
                     GWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLP
                     DDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLD
                     ARKIREILIKAKKGGKIAVNSHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARI
                     NV*"
     misc_feature    complement(2383..2598)
                     /label="SV40_enhancer"
                     /translation="MGEQIWPRRPSLDTRGYIMEARLPAWQSGTVGKATMEDAKNIKK
                     GPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAHIEVDITYAEYFEMSVRLAEAM
                     KRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPANDIYNERELLNSMGISQPTV
                     VFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVP
                     ESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVV
                     PFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKST
                     LIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGLTETTSAILITPEGDD
                     KPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKD
                     GWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLP
                     DDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLD
                     ARKIREILIKAKKGGKIAVNSHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARI
                     NV*"
     promoter        2395..2663
                     /label="SV40_promoter"
                     /translation="MGEQIWPRRPSLDTRGYIMEARLPAWQSGTVGKATMEDAKNIKK
                     GPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAHIEVDITYAEYFEMSVRLAEAM
                     KRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPANDIYNERELLNSMGISQPTV
                     VFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVP
                     ESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVV
                     PFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKST
                     LIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGLTETTSAILITPEGDD
                     KPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKD
                     GWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLP
                     DDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLD
                     ARKIREILIKAKKGGKIAVNSHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARI
                     NV*"
     rep_origin      2562..2639
                     /label="SV40_origin"
                     /translation="MGEQIWPRRPSLDTRGYIMEARLPAWQSGTVGKATMEDAKNIKK
                     GPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAHIEVDITYAEYFEMSVRLAEAM
                     KRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPANDIYNERELLNSMGISQPTV
                     VFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVP
                     ESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVV
                     PFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKST
                     LIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGLTETTSAILITPEGDD
                     KPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKD
                     GWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLP
                     DDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLD
                     ARKIREILIKAKKGGKIAVNSHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARI
                     NV*"
     misc_feature    2624..2643
                     /label="SV40pro_F_primer"
                     /translation="MGEQIWPRRPSLDTRGYIMEARLPAWQSGTVGKATMEDAKNIKK
                     GPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAHIEVDITYAEYFEMSVRLAEAM
                     KRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPANDIYNERELLNSMGISQPTV
                     VFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVP
                     ESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVV
                     PFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKST
                     LIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGLTETTSAILITPEGDD
                     KPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKD
                     GWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLP
                     DDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLD
                     ARKIREILIKAKKGGKIAVNSHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARI
                     NV*"
     CDS             2742..3779
                     /label="ORF frame 3"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKEVGRV*"
     terminator      3803..3851
                     /label="synth_PA_terminator"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKEVGRV*"
     rep_origin      complement(4163..4782)
                     /label="pBR322_origin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKEVGRV*"
     CDS             complement(4966..5826)
                     /label="ORF frame 1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    5931..6084
                     /label="TB"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     terminator      5931..5979
                     /label="synth_PA_terminator"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     terminator      5931..6084
                     /label="up_PA_terminator"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    6033..6052
                     /label="RVprimer3_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCGCACCAGA CAGTGACGTC AGCTGCCAGA
   61 TCCCATGGCC GTCATACTGT GACGTCTTTC AGACACCCCA TTGACGTCAA TGGGAGAACA
  121 GATCTGGCCT CGGCGGCCAA GCTTAGACAC TAGAGGGTAT ATAATGGAAG CTCGACTTCC
  181 AGCTTGGCAA TCCGGTACTG TTGGTAAAGC CACCATGGAA GATGCCAAAA ACATTAAGAA
  241 GGGCCCAGCG CCATTCTACC CACTCGAAGA CGGGACCGCC GGCGAGCAGC TGCACAAAGC
  301 CATGAAGCGC TACGCCCTGG TGCCCGGCAC CATCGCCTTT ACCGACGCAC ATATCGAGGT
  361 GGACATTACC TACGCCGAGT ACTTCGAGAT GAGCGTTCGG CTGGCAGAAG CTATGAAGCG
  421 CTATGGGCTG AATACAAACC ATCGGATCGT GGTGTGCAGC GAGAATAGCT TGCAGTTCTT
  481 CATGCCCGTG TTGGGTGCCC TGTTCATCGG TGTGGCTGTG GCCCCAGCTA ACGACATCTA
  541 CAACGAGCGC GAGCTGCTGA ACAGCATGGG CATCAGCCAG CCCACCGTCG TATTCGTGAG
  601 CAAGAAAGGG CTGCAAAAGA TCCTCAACGT GCAAAAGAAG CTACCGATCA TACAAAAGAT
  661 CATCATCATG GATAGCAAGA CCGACTACCA GGGCTTCCAA AGCATGTACA CCTTCGTGAC
  721 TTCCCATTTG CCACCCGGCT TCAACGAGTA CGACTTCGTG CCCGAGAGCT TCGACCGGGA
  781 CAAAACCATC GCCCTGATCA TGAACAGTAG TGGCAGTACC GGATTGCCCA AGGGCGTAGC
  841 CCTACCGCAC CGCACCGCTT GTGTCCGATT CAGTCATGCC CGCGACCCCA TCTTCGGCAA
  901 CCAGATCATC CCCGACACCG CTATCCTCAG CGTGGTGCCA TTTCACCACG GCTTCGGCAT
  961 GTTCACCACG CTGGGCTACT TGATCTGCGG CTTTCGGGTC GTGCTCATGT ACCGCTTCGA
 1021 GGAGGAGCTA TTCTTGCGCA GCTTGCAAGA CTATAAGATT CAATCTGCCC TGCTGGTGCC
 1081 CACACTATTT AGCTTCTTCG CTAAGAGCAC TCTCATCGAC AAGTACGACC TAAGCAACTT
 1141 GCACGAGATC GCCAGCGGCG GGGCGCCGCT CAGCAAGGAG GTAGGTGAGG CCGTGGCCAA
 1201 ACGCTTCCAC CTACCAGGCA TCCGCCAGGG CTACGGCCTG ACAGAAACAA CCAGCGCCAT
 1261 TCTGATCACC CCCGAAGGGG ACGACAAGCC TGGCGCAGTA GGCAAGGTGG TGCCCTTCTT
 1321 CGAGGCTAAG GTGGTGGACT TGGACACCGG TAAGACACTG GGTGTGAACC AGCGCGGCGA
 1381 GCTGTGCGTC CGTGGCCCCA TGATCATGAG CGGCTACGTT AACAACCCCG AGGCTACAAA
 1441 CGCTCTCATC GACAAGGACG GCTGGCTGCA CAGCGGCGAC ATCGCCTACT GGGACGAGGA
 1501 CGAGCACTTC TTCATCGTGG ACCGGCTGAA GAGCCTGATC AAATACAAGG GCTACCAGGT
 1561 AGCCCCAGCC GAACTGGAGA GCATCCTGCT GCAACACCCC AACATCTTCG ACGCCGGGGT
 1621 CGCCGGCCTG CCCGACGACG ATGCCGGCGA GCTGCCCGCC GCAGTCGTCG TGCTGGAACA
 1681 CGGTAAAACC ATGACCGAGA AGGAGATCGT GGACTATGTG GCCAGCCAGG TTACAACCGC
 1741 CAAGAAGCTG CGCGGTGGTG TTGTGTTCGT GGACGAGGTG CCTAAAGGAC TGACCGGCAA
 1801 GTTGGACGCC CGCAAGATCC GCGAGATTCT CATTAAGGCC AAGAAGGGCG GCAAGATCGC
 1861 CGTGAATTCT CACGGCTTCC CTCCCGAGGT GGAGGAGCAG GCCGCCGGCA CCCTGCCCAT
 1921 GAGCTGCGCC CAGGAGAGCG GCATGGATAG ACACCCTGCT GCTTGCGCCA GCGCCAGGAT
 1981 CAACGTCTAA GGCCGCGACT CTAGAGTCGG GGCGGCCGGC CGCTTCGAGC AGACATGATA
 2041 AGATACATTG ATGAGTTTGG ACAAACCACA ACTAGAATGC AGTGAAAAAA ATGCTTTATT
 2101 TGTGAAATTT GTGATGCTAT TGCTTTATTT GTAACCATTA TAAGCTGCAA TAAACAAGTT
 2161 AACAACAACA ATTGCATTCA TTTTATGTTT CAGGTTCAGG GGGAGGTGTG GGAGGTTTTT
 2221 TAAAGCAAGT AAAACCTCTA CAAATGTGGT AAAATCGATA AGGATCCGTT TGCGTATTGG
 2281 GCGCTCTTCC GCTGATCTGC GCAGCACCAT GGCCTGAAAT AACCTCTGAA AGAGGAACTT
 2341 GGTTAGCTAC CTTCTGAGGC GGAAAGAACC AGCTGTGGAA TGTGTGTCAG TTAGGGTGTG
 2401 GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG
 2461 CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC
 2521 TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC
 2581 CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG
 2641 AGGCCGCCTC TGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG
 2701 GCTTTTGCAA AAAGCTCGAT TCTTCTGACA CTAGCGCCAC CATGAAGAAG CCCGAACTCA
 2761 CCGCTACCAG CGTTGAAAAA TTTCTCATCG AGAAGTTCGA CAGTGTGAGC GACCTGATGC
 2821 AGTTGTCGGA GGGCGAAGAG AGCCGAGCCT TCAGCTTCGA TGTCGGCGGA CGCGGCTATG
 2881 TACTGCGGGT GAATAGCTGC GCTGATGGCT TCTACAAAGA CCGCTACGTG TACCGCCACT
 2941 TCGCCAGCGC TGCACTACCC ATCCCCGAAG TGTTGGACAT CGGCGAGTTC AGCGAGAGCC
 3001 TGACATACTG CATCAGTAGA CGCGCCCAAG GCGTTACTCT CCAAGACCTC CCCGAAACAG
 3061 AGCTGCCTGC TGTGTTACAG CCTGTCGCCG AAGCTATGGA TGCTATTGCC GCCGCCGACC
 3121 TCAGTCAAAC CAGCGGCTTC GGCCCATTCG GGCCCCAAGG CATCGGCCAG TACACAACCT
 3181 GGCGGGATTT CATTTGCGCC ATTGCTGATC CCCATGTCTA CCACTGGCAG ACCGTGATGG
 3241 ACGACACCGT GTCCGCCAGC GTAGCTCAAG CCCTGGACGA ACTGATGCTG TGGGCCGAAG
 3301 ACTGTCCCGA GGTGCGCCAC CTCGTCCATG CCGACTTCGG CAGCAACAAC GTCCTGACCG
 3361 ACAACGGCCG CATCACCGCC GTAATCGACT GGTCCGAAGC TATGTTCGGG GACAGTCAGT
 3421 ACGAGGTGGC CAACATCTTC TTCTGGCGGC CCTGGCTGGC TTGCATGGAG CAGCAGACTC
 3481 GCTACTTCGA GCGCCGGCAT CCCGAGCTGG CCGGCAGCCC TCGTCTGCGA GCCTACATGC
 3541 TGCGCATCGG CCTGGATCAG CTCTACCAGA GCCTCGTGGA CGGCAACTTC GACGATGCTG
 3601 CCTGGGCTCA AGGCCGCTGC GATGCCATCG TCCGCAGCGG GGCCGGCACC GTCGGTCGCA
 3661 CACAAATCGC TCGCCGGAGC GCAGCCGTAT GGACCGACGG CTGCGTCGAG GTGCTGGCCG
 3721 ACAGCGGCAA CCGCCGGCCC AGTACACGAC CGCGCGCTAA GGAGGTAGGT CGAGTTTAAA
 3781 CTCTAGAACC GGTCATGGCC GCAATAAAAT ATCTTTATTT TCATTACATC TGTGTGTTGG
 3841 TTTTTTGTGT GTTCGAACTA GATGCTGTCG ACCGATGCCC TTGAGAGCCT TCAACCCAGT
 3901 CAGCTCCTTC CGGTGGGCGC GGGGCATGAC TATCGTCGCC GCACTTATGA CTGTCTTCTT
 3961 TATCATGCAA CTCGTAGGAC AGGTGCCGGC AGCGCTCTTC CGCTTCCTCG CTCACTGACT
 4021 CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC
 4081 GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA
 4141 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG
 4201 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA
 4261 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC
 4321 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC
 4381 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC
 4441 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG
 4501 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT
 4561 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA
 4621 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT
 4681 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA
 4741 TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG
 4801 CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT
 4861 TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT
 4921 AAACTTGGTC TGACAGCGGC CGCAAATGCT AAACCACTGC AGTGGTTACC AGTGCTTGAT
 4981 CAGTGAGGCA CCGATCTCAG CGATCTGCCT ATTTCGTTCG TCCATAGTGG CCTGACTCCC
 5041 CGTCGTGTAG ATCACTACGA TTCGTGAGGG CTTACCATCA GGCCCCAGCG CAGCAATGAT
 5101 GCCGCGAGAG CCGCGTTCAC CGGCCCCCGA TTTGTCAGCA ATGAACCAGC CAGCAGGGAG
 5161 GGCCGAGCGA AGAAGTGGTC CTGCTACTTT GTCCGCCTCC ATCCAGTCTA TGAGCTGCTG
 5221 TCGTGATGCT AGAGTAAGAA GTTCGCCAGT GAGTAGTTTC CGAAGAGTTG TGGCCATTGC
 5281 TACTGGCATC GTGGTATCAC GCTCGTCGTT CGGTATGGCT TCGTTCAACT CTGGTTCCCA
 5341 GCGGTCAAGC CGGGTCACAT GATCACCCAT ATTATGAAGA AATGCAGTCA GCTCCTTAGG
 5401 GCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCGGTGTTG TCGCTCATGG TAATGGCAGC
 5461 ACTACACAAT TCTCTTACCG TCATGCCATC CGTAAGATGC TTTTCCGTGA CCGGCGAGTA
 5521 CTCAACCAAG TCGTTTTGTG AGTAGTGTAT ACGGCGACCA AGCTGCTCTT GCCCGGCGTC
 5581 TATACGGGAC AACACCGCGC CACATAGCAG TACTTTGAAA GTGCTCATCA TCGGGAATCG
 5641 TTCTTCGGGG CGGAAAGACT CAAGGATCTT GCCGCTATTG AGATCCAGTT CGATATAGCC
 5701 CACTCTTGCA CCCAGTTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CGGGGTGTGC
 5761 AAAAACAGGC AAGCAAAATG CCGCAAAGAA GGGAATGAGT GCGACACGAA AATGTTGGAT
 5821 GCTCATACTC GTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTACT AGTACGTCTC
 5881 TCAAGGATAA GTAAGTAATA TTAAGGTACG GGAGGTATTG GACAGGCCGC AATAAAATAT
 5941 CTTTATTTTC ATTACATCTG TGTGTTGGTT TTTTGTGTGA ATCGATAGTA CTAACATACG
 6001 CTCTCCATCA AAACAAAACG AAACAAAACA AACTAGCAAA ATAGGCTGTC CCCAGTGCAA
 6061 GTGCAGGTGC CAGAACATTT CTCT
//

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