| 质粒名称: | pTAL1 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 31031 |
| 存储实验室: | Adam Bogdanove, Daniel Voytas |
|
载体骨架基本信息 |
|
| 载体名称: | pCR8-GW modified |
| 载体出品公司: | Invitrogen |
| 载体抗性: | 氨苄青霉素（50 ug/ml） |
| 空载体大小: | 2676 bp |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | TALE |
| 物种来源: | Xanthamonas oryzae |
| 插入大小: | 2101 bp |
| 克隆方法: | 限制性内切酶 |
| 5' 测序引物 | TAL_F1 (ttggcgtcggcaaacagtgg) |
| 3' 测序引物 | TAL_R2 (ggcgacgaggtggtcgttgg) |
|
文献引用方法 |
|
| 材料和方法部分: | pTAL1 was a gift from Adam Bogdanove & Daniel Voytas (Addgene plasmid # 31031 ; http://n2t.net/addgene:31031 ; RRID:Addgene_31031) |
| 参考文献部分: | Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove AJ, Voytas DF. Nucleic Acids Res. 2011 Apr 14. ():. 10.1093/nar/gkr218 PubMed 21493687 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pTAL1 4777 bp ds-DNA circular SYN 13-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pTAL1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4777)
AUTHORS Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller
JA, Somia NV, Bogdanove AJ, Voytas DF
TITLE Efficient design and assembly of custom TALEN and other TAL
effector-based constructs for DNA targeting.
JOURNAL Nucleic Acids Res. 2011 Apr 14. ():.
PUBMED 21493687
REFERENCE 2 (bases 1 to 4777)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4777
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 1..17
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 33..132
/gene="mutant version of attL"
/label=attL1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
CDS complement(927..1334)
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/label=lacZ-alpha
/translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGARLQGG
TRQCIARCMHCLHCGHAATFCNLHKLACVQL"
primer_bind 1199..1215
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 1225..1243
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(1291..1311)
/label=T3
/note="T3 promoter, forward primer"
promoter complement(1291..1309)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(1330..1346)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(1343..1365)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 1354..1370
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(1378..1408)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 1423..1444
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
protein_bind complement(2285..2384)
/gene="mutant version of attL"
/label=attL2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
promoter complement(2402..2420)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(2425..2441)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
CDS complement(2603..3463)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 3226..3245
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 3591..4179
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4080..4099
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 4333..4350
/label=L4440
/note="L4440 vector, forward primer"
terminator 4509..4536
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator 4628..4714
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
ORIGIN
1 gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag
61 tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca
121 aaaaagcagg ctccgaattc gcccttttgc atgtaaatag gaggtgcacc atggatccca
181 ttcgtccgcg caggccaagt cctgcccgcg agcttctgcc cggaccccaa ccggataggg
241 ttcagccgac tgcagatcgt ggggtgtctg cgcctgctgg cagccctctg gatggcttgc
301 ccgctcggcg gacggtgtcc cggacccggc tgccatctcc ccctgcgccc tcacctgcgt
361 tctcggcggg cagcttcagc gatctgctcc gtccgttcga tccgtcgctt cttgatacat
421 cgcttcttga ttcgatgcct gccgtcggca cgccgcatac agcggctgcc ccagcagagt
481 gggatgaggc gcaatcggct ctgcgtgcag ccgatgaccc gccacccacc gtgcgtgtcg
541 ctgtcactgc cgcgcggccg ccgcgcgcca agccggcccc gcgacggcgt gctgcgcaac
601 cctccgacgc ttcgccggcc gcgcaggtgg atctacgcac gctcggctac agtcagcagc
661 agcaagagaa gatcaaaccg aaggtgcgtt cgacagtggc gcagcaccac gaggcactgg
721 tgggccatgg gtttacacac gcgcacatcg ttgcgctcag ccaacacccg gcagcgttag
781 ggaccgtcgc tgtcacgtat cagcacataa tcacggcgtt gccagaggcg acacacgaag
841 acatcgttgg cgtcggcaaa cagtggtccg gcgcacgcgc cctggaggcc ttgctcacgg
901 atgcggggga gttgagaggt ccgccgttac agttggacac aggccaactt gtgaagattg
961 caaaacgtgg cggcgtgacc gcaatggagg cagtgcatgc atcgcgcaat gcactgacgg
1021 gtgcccccct ggagacgggc gccgctacag ggcgcgtccc attcgccatt caggctgcgc
1081 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
1141 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
1201 aaaacgacgg ccagtgagcg cgcgtaatac gactcactat agggcgaatt gggtaccggg
1261 ccccccctcg aggtcctcca gcttttgttc cctttagtga gggttaattg cgcgcttggc
1321 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
1381 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
1441 attaattgcg ttgcgctcac tgcccgcttt ccaccggtcg tctccaacga ccacctcgtc
1501 gccttggcct gcctcggcgg acgtcctgcc atggatgcag tgaaaaaggg attgccgcac
1561 gcgccggaat tgatcagaag agtcaatcgc cgtattggcg aacgcacgtc ccatcgcgtt
1621 gccgactacg cgcaagtggt tcgcgtgctg gagtttttcc agtgccactc ccacccagcg
1681 tacgcatttg atgaggccat gacgcagttc gggatgagca ggaacgggtt ggtacagctc
1741 tttcgcagag tgggcgtcac cgaactcgaa gcccgcggtg gaacgctccc cccagcctcg
1801 cagcgttggg accgtatcct ccaggcatca gggatgaaaa gggccaaacc gtcccctact
1861 tcagctcaaa caccggatca ggcgtctttg catgcattcg ccgattcgct ggagcgtgac
1921 cttgatgcgc ccagcccaat gcacgaggga gatcagacgc gggcaagcag ccgtaaacgg
1981 tcccgatcgg atcgtgctgt caccggcccc tccgcacagc aggctgtcga ggtgcgcgtt
2041 cccgaacagc gcgatgcgct gcatttgccc ctcagctgga gggtaaaacg cccgcgtacc
2101 aggatctggg gcggcctccc ggatcctggt acgcccatgg ctgccgacct ggcagcgtcc
2161 agcaccgtga tgtgggaaca agatgcggac cccttcgcag gggcagcgga tgatttcccg
2221 gcattcaacg aagaggaact cgcatggttg atggagctat tgcctcagtg aaagggcgaa
2281 ttcgacccag ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt
2341 tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc
2401 ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct
2461 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaga ttatcaaaaa
2521 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
2581 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
2641 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
2701 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
2761 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
2821 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
2881 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
2941 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
3001 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
3061 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
3121 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
3181 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
3241 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
3301 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
3361 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
3421 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
3481 attattgaag catttatcag ggttattgtc tcatgaccaa aatcccttaa cgtgagttac
3541 gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
3601 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
3661 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
3721 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
3781 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
3841 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
3901 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
3961 ctgagatacc tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg
4021 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
4081 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
4141 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
4201 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
4261 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
4321 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
4381 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
4441 aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta
4501 gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt
4561 atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc
4621 ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg
4681 cccagtcttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgcg
4741 ttaacgctag catggatgtt ttcccagtca cgacgtt
//
