pET-37b(+)

价格:1000元

联系方式:I47-825O-882O

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pET37b载体基本信息

出品公司: EMD Biosciences (Novagen)
别名: pET37b, pet 37b, pET-37b(+)
质粒类型: 大肠杆菌蛋白表达
表达水平:
克隆方法: 多克隆位点,限制性内切酶
载体大小: 5932 bp
5' 测序引物及序列: T7: 5'-TAATACGACTCACTATAGGG-3'
3' 测序引物序列:   T7t: 5'-GCTAGTTATTGCTCAGCGG-3'
载体标签: N-Signal, N-CBD, N-S, N-Thrombin, N-EK, C-His
载体抗性: 卡那
表达宿主菌: BL21(DE3) Rosetta2(DE3)C43(DE3)
备注: 载体含有N端纤维素结合结构域(N-CBD), 是分泌性蛋白表达载体。
产品目录号:  
稳定性: 瞬时表达
组成型: 组成型
病毒/非病毒: 非病毒

pET37b载体质粒图谱和多克隆位点信息

pet37b载体图谱

pET37b载体简介

pET Vectors 选择指南

pET37b载体序列

LOCUS       pET-37b(+)              5932 bp    DNA     circular     23-MAY-2002
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     promoter        complement(929..945)
                     /vntifkey="30"
                     /label=T7\promoter
     misc_signal     complement(729..857)
                     /vntifkey="87"
                     /label=Signal\sequence
     primer_bind     complement(386..730)
                     /vntifkey="28"
                     /label=CBD(cenA)\tag
     protein_bind    complement(291..335)
                     /vntifkey="31"
                     /label=S\tag
     protein_bind    complement(150..173)
                     /vntifkey="31"
                     /label=His\tag
     terminator      complement(26..72)
                     /vntifkey="43"
                     /label=T7\terminator
     CDS             1336..2415
                     /vntifkey="4"
                     /label=lac\I
     rep_origin      complement(3849..3849)
                     /vntifkey="33"
                     /label=ColE1\pBR322
     CDS             4558..5370
                     /vntifkey="4"
                     /label=kan\sequence
     rep_origin      complement(5466..5921)
                     /vntifkey="33"
                     /label=f1\origin
     protein_bind    complement(351..368)
                     /vntifkey="31"
                     /label=thrombin
     protein_bind    complement(249..260)
                     /vntifkey="31"
                     /label=factor\Xa
     misc_feature    complement(249..249)
                     /vntifkey="21"
                     /label=LIC\site
     CDS             complement(902..926)
                     /vntifkey="4"
                     /label=lac\operator
BASE COUNT     1353 a      1555 c      1669 g      1355 t 
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 
      121 tgtttagcag cctaggtatt aatcaattag tggtggtggt ggtggtggtg gtgctcgagt 
      181 gcggccgcaa gcttgtcgac ggagctcgaa ttcggatccg atatcgccat ggccagagga 
      241 gagttagagc gaccctcaat accggagcca ccaccggtac ccagatctgg gctgtccatg 
      301 tgctggcgtt cgaatttagc agcagcggtt tctttcatac caattgcagt actaccgcgt 
      361 ggcaccagac ccgcggaacc tggtgccgtg gggctggtcg tcggcacggt gcccgtgcag 
      421 gtggtgccgt tgagcgagaa cgacgccggc gtcgggttgg acccggccca cgagccgttg 
      481 aacccgaacg acgccgtgcc gccggtcggg atgctgccgt tccagggcag gctggtgacg 
      541 gagacctggc cgccgttggt cgaggcggtg ccgttccaca gctgctggat acgctggcct 
      601 gcggtgtagg tccagtcgag cttccacgac gagacggggt cgccgaggtt ggtgatcgtg 
      661 acgttggcgc cgaagccgcc gggccactgg ttggtgacgg cgtagtcgac gcggcagccg 
      721 ggactagcgg cctgggcggg cagcacgacc gtggcgccga cgacgagcgt cgccgccgcg 
      781 gcggcgagcg tcgtgcgcag agcggtgcgg gcgccgcggg ccgggtggcc gggtgcgggc 
      841 gtggtacgag ggtccatatg tatatctcct tcttaaagtt aaacaaaatt atttctagag 
      901 gggaattgtt atccgctcac aattccccta tagtgagtcg tattaatttc gcgggatcga 
      961 gatcgatctc gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg 
     1021 cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg 
     1081 ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt 
     1141 tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc 
     1201 tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga gatcccggac 
     1261 accatcgaat ggcgcaaaac ctttcgcggt atggcatgat agcgcccgga agagagtcaa 
     1321 ttcagggtgg tgaatgtgaa accagtaacg ttatacgatg tcgcagagta tgccggtgtc 
     1381 tcttatcaga ccgtttcccg cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg 
     1441 gaaaaagtgg aagcggcgat ggcggagctg aattacattc ccaaccgcgt ggcacaacaa 
     1501 ctggcgggca aacagtcgtt gctgattggc gttgccacct ccagtctggc cctgcacgcg 
     1561 ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc cagcgtggtg 
     1621 gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc 
     1681 gcgcaacgcg tcagtgggct gatcattaac tatccgctgg atgaccagga tgccattgct 
     1741 gtggaagctg cctgcactaa tgttccggcg ttatttcttg atgtctctga ccagacaccc 
     1801 atcaacagta ttattttctc ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc 
     1861 gcattgggtc accagcaaat cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt 
     1921 ctgcgtctgg ctggctggca taaatatctc actcgcaatc aaattcagcc gatagcggaa 
     1981 cgggaaggcg actggagtgc catgtccggt tttcaacaaa ccatgcaaat gctgaatgag 
     2041 ggcatcgttc ccactgcgat gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc 
     2101 gccattaccg agtccgggct gcgcgttggt gcggacatct cggtagtggg atacgacgat 
     2161 accgaagaca gctcatgtta tatcccgccg ttaaccacca tcaaacagga ttttcgcctg 
     2221 ctggggcaaa ccagcgtgga ccgcttgctg caactctctc agggccaggc ggtgaagggc 
     2281 aatcagctgt tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc caatacgcaa 
     2341 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga 
     2401 ctggaaagcg ggcagtgagc gcaacgcaat taatgtaagt tagctcactc attaggcacc 
     2461 gggatctcga ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg 
     2521 gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca 
     2581 ggtgccggca gcgctctggg tcattttcgg cgaggaccgc tttcgctgga gcgcgacgat 
     2641 gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac 
     2701 tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt atcgccggca tggcggcccc 
     2761 acgggtgcgc atgatcgtgc tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct 
     2821 tactggttag cagaatgaat caccgatacg cgagcgaacg tgaagcgact gctgctgcaa 
     2881 aacgtctgcg acctgagcaa caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct 
     2941 ggaaacgcgg aagtcagcgc cctgcaccat tatgttccgg atctgcatcg caggatgctg 
     3001 ctggctaccc tgtggaacac ctacatctgt attaacgaag cgctggcatt gaccctgagt 
     3061 gatttttctc tggtcccgcc gcatccatac cgccagttgt ttaccctcac aacgttccag 
     3121 taaccgggca tgttcatcat cagtaacccg tatcgtgagc atcctctctc gtttcatcgg 
     3181 tatcattacc cccatgaaca gaaatccccc ttacacggag gcatcagtga ccaaacagga 
     3241 aaaaaccgcc cttaacatgg cccgctttat cagaagccag acattaacgc ttctggagaa 
     3301 actcaacgag ctggacgcgg atgaacaggc agacatctgt gaatcgcttc acgaccacgc 
     3361 tgatgagctt taccgcagct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca 
     3421 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 
     3481 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg 
     3541 tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga 
     3601 gtgcaccata tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca 
     3661 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag 
     3721 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 
     3781 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 
     3841 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 
     3901 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 
     3961 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 
     4021 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 
     4081 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 
     4141 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 
     4201 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 
     4261 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc 
     4321 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 
     4381 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 
     4441 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 
     4501 ttttggtcat gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg 
     4561 agccatattc aacgggaaac gtcttgctct aggccgcgat taaattccaa catggatgct 
     4621 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat 
     4681 cgattgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt 
     4741 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt 
     4801 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc 
     4861 cccgggaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt 
     4921 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt 
     4981 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt 
     5041 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa 
     5101 atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt 
     5161 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga 
     5221 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct 
     5281 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg 
     5341 cagtttcatt tgatgctcga tgagtttttc taagaattaa ttcatgagcg gatacatatt 
     5401 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 
     5461 acctgaaatt gtaaacgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag 
     5521 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac 
     5581 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga 
     5641 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc 
     5701 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg 
     5761 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa 
     5821 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac 
     5881 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc ca 
//

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pET37b载体应用举例

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