| 出品公司: | Novagen |
|---|---|
| 载体名称: | pET-51b(+) , pET51b , pET51b载体 , pET-51b载体 |
| 质粒类型: | 大肠杆菌表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | T7 |
| 载体大小: | 5.2 kb |
| 5’测序引物及序列: | T7: 5'-TAATACGACTCACTATAGGG-3' |
| 3’测序引物及序列: | T7t: 5'-GCTAGTTATTGCTCAGCGG-3' |
| 载体标签: | N-Strep tag , C-His tag |
| 载体抗性: | 氨苄青霉素 |
| 克隆菌株: | DH5 alpha |
| 表达细胞: | BL21(DE3), Rosetta(DE3), OrigamiB(DE3) |
| 备注: | |
| 产品目录号: | 71553 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
| 表达宿主菌: | BL21(DE3) 、BLR(DE3)、Rosetta(DE3)、OrigamiB(DE3) |
| pET表达手册： |
pET表达手册英文版 pET表达手册中文版 |
| pET载体汇总: |
pET-3a pET-11a pET-12a pET-14b pET-15b pET-16b pET-17b pET-19b pET-20b pET-21a pET-22b pET-23a pET-24a pET-25b pET-26b pET-27b pET-28a pET-29a pET-30a pET-31b pET-32a pET-33b pET-34b pET-35b pET-37b pET-39b pET-40b pET-41a pET-42a pET-43.1a pET-44a pET-45b pET-47b pET-48b pET-49b pET-50b pET-51b pET-52b pETDuet-1 pACYCDuet-1 pCDFDuet-1 pRSFDuet-1 pColaDuet-1 |
买家导航
pET51b载体基本信息
pET51b载体图谱质粒图谱和多克隆位点信息
pET51b载体简介
pET-51b（+）载体携带N-Strep Tag，N-EK蛋白酶位点和可选的C-His Tag。存在N端和C端的两个纯化标签是双重纯化策略，本设计用于分离全长融合蛋白。pET51b载体的单一的多克隆位点见上面的环状质粒图谱。注意：载体序列是以pBR322质粒的编码规矩进行编码的，所以T7蛋白表达区在质粒图谱上面是反向的。
T7 RNA聚合酶启动的克隆和表达区域在质粒图谱中也被标注了出来。质粒的F1复制子是被定向的，所以在T7噬菌体聚合酶的作用下，包含有蛋白编码序列的病毒粒子能够产生，并启动蛋白表达，同时蛋白表达将被T7终止子序列(Cat. No. 69337-3)的作用下终止蛋白翻译。
pET51b载体序列
LOCUS pET-51b(+) 5218 bp DNA circular 1-SEP-2005 DEFINITION Reverse complement of pET-51b. SOURCE ORGANISM COMMENT VNTNAME|pET-51b(+) rc| COMMENT VNTREPLTYPE|Plasmid COMMENT VNTEXTCHREPL|Bacteria COMMENT http://www.biofeng.com FEATURES Location/Qualifiers misc_RNA 3456..3563 /vntifkey="53" /label=RNA\I misc_RNA complement(3016..3571) /vntifkey="53" /label=RNA\II CDS 2394..2585 /vntifkey="4" /label=ROP RBS complement(270..275) /vntifkey="32" /label=RBS-1 primer complement(380..395) /vntifkey="27" /label=pET\Upstream\Primer promoter complement(333..349) /vntifkey="30" /label=T7\promoter CDS 740..1822 /vntifkey="4" /label=lac\I rep_origin complement(3016..3016) /vntifkey="33" /label=ColE1\pBR322\origin CDS 3777..4637 /vntifkey="4" /label=bla\(amp)\sequence rep_origin complement(4759..5206) /vntifkey="33" /label=f1\origin CDS complement(308..329) /vntifkey="4" /label=lac\operator terminator complement(26..73) /vntifkey="43" /label=T7\Terminator -35_signal complement(3598..3603) /vntifkey="48" /label=-35\RNA\II -10_signal complement(3575..3580) /vntifkey="47" /label=-10\RNA\II misc_RNA complement(3016..3567) /vntifkey="53" /label=RNA\II -35_signal 3421..3426 /vntifkey="48" /label=-35\RNA\I -10_signal 3445..3450 /vntifkey="47" /label=-10\RNA\I misc_RNA 3457..3593 /vntifkey="53" /label=RNA\I terminator complement(3594..3612) /vntifkey="43" /label=RNA\I\Terminator misc_feature complement(149..163) /vntifkey="21" /label=C-Term\Ek/LIC\overhang protein_bind complement(209..223) /vntifkey="31" /label=Ek\site protein_bind complement(230..253) /vntifkey="31" /label=Strep-Tag\II misc_binding complement(109..139) /vntifkey="20" /label=10x\His-Tag primer complement(146..229) /vntifkey="27" /label=S-EkLIC\MCS primer 140..223 /vntifkey="27" /label=AS-EkLIC\MCS primer complement(230..263) /vntifkey="27" /label=S-NTerm\Strep primer 224..259 /vntifkey="27" /label=AS-NTerm\Strep BASE COUNT 1260 a 1352 c 1398 g 1208 t ORIGIN 1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 61 ggggttatgc tagttattgc tcagcggtgg cagcagccta ggttaattag tggtggtgat 121 ggtgatgatg gtggtgatga gcggaaattg aggagaagcc cggagctcct gcggccgcaa 181 gcttgtcgac cgacgtcgga tccggtacct tgtcgtcgtc atctgcaccc ttttcgaact 241 gcgggtggct ccagcttgcc atggtatatc tccttcttaa agttaaacaa aattatttct 301 agaggggaat tgttatccgc tcacaattcc cctatagtga gtcgtattaa tttcgcggga 361 tcgagatcga tctcgatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca 421 ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac 481 ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga 541 ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc 601 aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgagatccc 661 ggacaccatc gaatggcgca aaacctttcg cggtatggca tgatagcgcc cggaagagag 721 tcaattcagg gtggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg 781 tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac 841 gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca 901 acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca 961 cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt 1021 ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct 1081 tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat 1141 tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac 1201 acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct 1261 ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc 1321 gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc 1381 ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa 1441 tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat 1501 gcgcgccatt accgagtccg ggctgcgcgt tggtgcggac atctcggtag tgggatacga 1561 cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg 1621 cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa 1681 gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac 1741 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc 1801 ccgactggaa agcgggcagt gagcgcaacg caattaatgt aagttagctc actcattagg 1861 caccgggatc tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg 1921 cgcggggcat gactagcatg atcgtgctcc tgtcgttgag gacccggcta ggctggcggg 1981 gttgccttac tggttagcag aatgaatcac cgatacgcga gcgaacgtga agcgactgct 2041 gctgcaaaac gtctgcgacc tgagcaacaa catgaatggt cttcggtttc cgtgtttcgt 2101 aaagtctgga aacgcggaag tcagcgccct gcaccattat gttccggatc tgcatcgcag 2161 gatgctgctg gctaccctgt ggaacaccta catctgtatt aacgaagcgc tggcattgac 2221 cctgagtgat ttttctctgg tcccgccgca tccataccgc cagttgttta ccctcacaac 2281 gttccagtaa ccgggcatgt tcatcatcag taacccgtat cgtgagcatc ctctctcgtt 2341 tcatcggtat cattaccccc atgaacagaa atccccctta cacggaggca tcagtgacca 2401 aacaggaaaa aaccgccctt aacatggccc gctttatcag aagccagaca ttaacgcttc 2461 tggagaaact caacgagctg gacgcggatg aacaggcaga catctgtgaa tcgcttcacg 2521 accacgctga tgagctttac cgcagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc 2581 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca 2641 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc 2701 agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt 2761 actgagagtg caccatatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac 2821 cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 2881 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 2941 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 3001 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 3061 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 3121 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 3181 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 3241 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 3301 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 3361 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 3421 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg 3481 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 3541 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 3601 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 3661 taagggattt tggtcatgac ctatttgttt atttttctaa atacattcaa atatgtatcc 3721 gctcatgaga caataaccct gataaatgct tcaatcatga ttgaaaaagg aagagtatga 3781 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt 3841 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag 3901 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag 3961 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta 4021 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg 4081 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca 4141 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag 4201 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc 4261 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg 4321 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc 4381 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg 4441 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg 4501 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga 4561 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac 4621 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa 4681 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgccat 4741 cgtgccgcca gaaattgtaa acgttaatat tttgttaaaa ttcgcgttaa atttttgtta 4801 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga 4861 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa 4921 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga 4981 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc 5041 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga 5101 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg 5161 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgcca //
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