| 质粒名称: | pCpf1-sp |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 122186 |
| 存储实验室: | Cheng-Cai Zhang |
|
载体骨架基本信息 |
|
| 载体名称: | 未知 |
| 完整质粒大小: | 12595bp |
| 载体类型: | 细菌细胞表达、CRISPR |
| 载体抗性: | Spectinomycin |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 克隆方法: | Ligation Independent Cloning |
|
文献引用方法 |
|
| 材料和方法部分: | pCpf1-sp was a gift from Cheng-Cai Zhang (Addgene plasmid # 122186 ; http://n2t.net/addgene:122186 ; RRID:Addgene_122186) |
| 参考文献部分: |
Expanding the potential of CRISPR-Cpf1 based genome editing technology in the cyanobacterium Anabaena PCC 7120. Niu TC, Lin GM, Xie LR, Wang ZQ, Xing WY, Zhang JY, Zhang CC. ACS Synth Biol. 2018 Dec 10. doi: 10.1021/acssynbio.8b00437. 10.1021/acssynbio.8b00437 PubMed 30525474
Cloned using mAzurite-C1 (#54583)
|
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCpf1-sp 12595 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Bacterial genome editing plasmid with spectinomycin resistance
marker.
ACCESSION .
VERSION .
KEYWORDS pCpf1-sp
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12595)
AUTHORS Niu TC, Lin GM, Xie LR, Wang ZQ, Xing WY, Zhang JY, Zhang CC
TITLE Expanding the potential of CRISPR-Cpf1 based genome editing
technology in the cyanobacterium Anabaena PCC 7120.
JOURNAL ACS Synth Biol. 2018 Dec 10. doi: 10.1021/acssynbio.8b00437.
PUBMED 30525474
REFERENCE 2 (bases 1 to 12595)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..12595
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 3923..3957
/label=J23119 promoter
/note="bacterial promoter (Registry of Standard Biological
Parts BBa_J23119)"
CDS complement(4025..4321)
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/label=lacZ-alpha
/translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSG"
primer_bind 4171..4193
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 4185..4202
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 4186..4202
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 4212..4231
/label=T7
/note="T7 promoter, forward primer"
promoter 4212..4230
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(4278..4298)
/label=T3
/note="T3 promoter, forward primer"
promoter complement(4278..4296)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(4317..4333)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4317..4333)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4330..4352)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4341..4357
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4365..4395)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4410..4431
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
CDS 5141..5932
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
rep_origin 6711..7105
/label=RSF1010 oriV
/note="replication origin of the broad-host-range plasmid
RSF1010; requires the RSF1010 RepA/B/C proteins for
replication (Scholz et al., 1989)"
oriT 7447..7534
/label=RSF1010 oriT
/note="origin of transfer of the broad-host-range plasmid
RSF1010 (Scholz et al., 1989)"
CDS 8773..9744
/codon_start=1
/gene="repB"
/product="replication protein B of the broad-host-range
plasmid RSF1010 (Scholz et al., 1989)"
/label=RSF1010 RepB
/translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
CDS 10255..11094
/codon_start=1
/gene="repA"
/product="replication protein A of the broad-host-range
plasmid RSF1010 (Scholz et al., 1989)"
/label=RSF1010 RepA
/translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
primer_bind 12442..12461
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter 12565..12594
/label=lac promoter
/note="promoter for the E. coli lac operon"
ORIGIN
1 ggaggtcttt atcatgtcaa tttatcaaga atttgttaat aaatatagtt taagtaaaac
61 tctaagattt gagttaatcc cacagggtaa aacacttgaa aacataaaag caagaggttt
121 gattttagat gatgagaaaa gagctaaaga ctacaaaaag gctaaacaaa taattgataa
181 atatcatcag ttttttatag aggagatatt aagttcggtt tgtattagcg aagatttatt
241 acaaaactat tctgatgttt attttaaact taaaaagagt gatgatgata atctacaaaa
301 agattttaaa agtgcaaaag atacgataaa gaaacaaata tctgaatata taaaggactc
361 agagaaattt aagaatttgt ttaatcaaaa ccttatcgat gctaaaaaag ggcaagagtc
421 agatttaatt ctatggctaa agcaatctaa ggataatggt atagaactat ttaaagccaa
481 tagtgatatc acagatatag atgaggcgtt agaaataatc aaatctttta aaggttggac
541 aacttatttt aagggttttc atgaaaatag aaaaaatgtt tatagtagca atgatattcc
601 tacatctatt atttatagga tagtagatga taatttgcct aaatttctag aaaataaagc
661 taagtatgag agtttaaaag acaaagctcc agaagctata aactatgaac aaattaaaaa
721 agatttggca gaagagctaa cctttgatat tgactacaaa acatctgaag ttaatcaaag
781 agttttttca cttgatgaag tttttgagat agcaaacttt aataattatc taaatcaaag
841 tggtattact aaatttaata ctattattgg tggtaaattt gtaaatggtg aaaatacaaa
901 gagaaaaggt ataaatgaat atataaatct atactcacag caaataaatg ataaaacact
961 caaaaaatat aaaatgagtg ttttatttaa gcaaatttta agtgatacag aatctaaatc
1021 ttttgtaatt gataagttag aagatgatag tgatgtagtt acaacgatgc aaagttttta
1081 tgagcaaata gcagctttta aaacagtaga agaaaaatct attaaagaaa cactatcttt
1141 attatttgat gatttaaaag ctcaaaaact tgatttgagt aaaatttatt ttaaaaatga
1201 taaatctctt actgatctat cacaacaagt ttttgatgat tatagtgtta ttggtacagc
1261 ggtactagaa tatataactc aacaaatagc acctaaaaat cttgataacc ctagtaagaa
1321 agagcaagaa ttaatagcca aaaaaactga aaaagcaaaa tacttatctc tagaaactat
1381 aaagcttgcc ttagaagaat ttaataagca tagagatata gataaacagt gtaggtttga
1441 agaaatactt gcaaactttg cggctattcc gatgatattt gatgaaatag ctcaaaacaa
1501 agacaatttg gcacagatat ctatcaaata tcaaaatcaa ggtaaaaaag acctacttca
1561 agctagtgcg gaagatgatg ttaaagctat caaggatctt ttagatcaaa ctaataatct
1621 cttacataaa ctaaaaatat ttcatattag tcagtcagaa gataaggcaa atattttaga
1681 caaggatgag catttttatc tagtatttga ggagtgctac tttgagctag cgaatatagt
1741 gcctctttat aacaaaatta gaaactatat aactcaaaag ccatatagtg atgagaaatt
1801 taagctcaat tttgagaact cgactttggc taatggttgg gataaaaata aagagcctga
1861 caatacggca attttattta tcaaagatga taaatattat ctgggtgtga tgaataagaa
1921 aaataacaaa atatttgatg ataaagctat caaagaaaat aaaggcgagg gttataaaaa
1981 aattgtttat aaacttttac ctggcgcaaa taaaatgtta cctaaggttt tcttttctgc
2041 taaatctata aaattttata atcctagtga agatatactt agaataagaa atcattccac
2101 acatacaaaa aatggtagtc ctcaaaaagg atatgaaaaa tttgagttta atattgaaga
2161 ttgccgaaaa tttatagatt tttataaaca gtctataagt aagcatccgg agtggaaaga
2221 ttttggattt agattttctg atactcaaag atataattct atagatgaat tttatagaga
2281 agttgaaaat caaggctaca aactaacttt tgaaaatata tcagagagct atattgatag
2341 cgtagttaat cagggtaaat tgtacctatt ccaaatctat aataaagatt tttcagctta
2401 tagcaaaggg cgaccaaatc tacatacttt atattggaaa gcgctgtttg atgagagaaa
2461 tcttcaagat gtggtttata agctaaatgg tgaggcagag cttttttatc gtaaacaatc
2521 aatacctaaa aaaatcactc acccagctaa agaggcaata gctaataaaa acaaagataa
2581 tcctaaaaaa gagagtgttt ttgaatatga tttaatcaaa gataaacgct ttactgaaga
2641 taagtttttc tttcactgtc ctattacaat caattttaaa tctagtggag ctaataagtt
2701 taatgatgaa atcaatttat tgctaaaaga aaaagcaaat gatgttcata tattaagtat
2761 agatagaggt gaaagacatt tagcttacta tactttggta gatggtaaag gcaatatcat
2821 caaacaagat actttcaaca tcattggtaa tgatagaatg aaaacaaact accatgataa
2881 gcttgctgca atagagaaag atagggattc agctaggaaa gactggaaaa agataaataa
2941 catcaaagag atgaaagagg gctatctatc tcaggtagtt catgaaatag ctaagctagt
3001 tatagagtat aatgctattg tggtttttga ggatttaaat tttggattta aaagagggcg
3061 tttcaaggta gagaagcagg tctatcaaaa gttagaaaaa atgctaattg agaaactaaa
3121 ctatctagtt ttcaaagata atgagtttga taaaactggg ggagtgctta gagcttatca
3181 gctaacagca ccttttgaga cttttaaaaa gatgggtaaa caaacaggta ttatctacta
3241 tgtaccagct ggttttactt caaaaatttg tcctgtaact ggttttgtaa atcagttata
3301 tcctaagtat gaaagtgtca gcaaatctca agagttcttt agtaagtttg acaagatttg
3361 ttataacctt gataagggct attttgagtt tagttttgat tataaaaact ttggtgacaa
3421 ggctgccaaa ggcaagtgga ctatagctag ctttgggagt agattgatta actttagaaa
3481 ttcagataaa aatcataatt gggatactcg agaagtttat ccaactaaag agttggagaa
3541 attgctaaaa gattattcta tcgaatatgg gcatggcgaa tgtatcaaag cagctatttg
3601 cggtgagagc gacaaaaagt tttttgctaa gctaactagt gtcctaaata ctatcttaca
3661 aatgcgtaac tcaaaaacag gtactgagtt agattatcta atttcaccag tagcagatgt
3721 aaatggcaat ttctttgatt cgcgacaggc gccaaaaaat atgcctcaag atgctgatgc
3781 caatggtgct tatcatattg ggctaaaagg tctgatgcta ctaggtagga tcaaaaataa
3841 tcaagagggc aaaaaactca atttggttat caaaaatgaa gagtattttg agttcgtgca
3901 gaataggaat aactaagaat tcttgacagc tagctcagtc ctaggtataa tgctagcgct
3961 gatttaggca aaaacgggtc taagaacttt aaataatttc tactgttgta gatgagagca
4021 ggtgtcagcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag
4081 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
4141 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
4201 gtgagcgcgc gtaatacgac tcactatagg gcgaattggg taccgggccc cccctcgagg
4261 tcctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca
4321 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
4381 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
4441 cgctcactgc ccgctttcca cccacctgca aaagtctaag aactttaaat aatttctact
4501 gttgtagatg ctactattcc tgtgccttca gataattcag tctaagaact ttaaataatt
4561 tctactgttg tagatgtcta gagccttttg tattagtagc cggtctaaga actttaaata
4621 atttctactg ttgtagatta gcgatttatg aaggtcattt ttttgtctag ctttaatgcg
4681 gtagttggta ccgtcgatgg cagaaattcg atatctagat ctcatggatc cgcaatggca
4741 acaacgttgc gctcgactct agaagaccga aaccttgcgc tcgttcgcca gccaggacag
4801 aaatgcctcg acttcgctgc tgcccaaggt tgccgggtga cgcacaccgt ggaaacggat
4861 gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat gcaagtagcg
4921 tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt
4981 ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc
5041 caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt
5101 tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc
5161 cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac
5221 gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga
5281 tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat
5341 caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga
5401 agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact
5461 gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat
5521 cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg
5581 tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa
5641 tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt
5701 gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt
5761 cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc
5821 tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga
5881 agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat aatgtctaac
5941 aattcgttca agccgacgcc gcttcgcggc gcggcttaac tcaagcgtta gatgcactaa
6001 gcacataatt gctcacagcc aaactatcag gtcaagtctg cttttattat ttttaagcgt
6061 gcataataag ccctacacaa attgggagat atatcatgaa aggctggctt tttcttgtta
6121 tcgcaatagt tggcgaagta atcgcaacat ccgcattaaa atctagcgag ggctttacta
6181 agctgatccg gtggatgacc ttttgaatga cctttaatag attatattac taattaattg
6241 gggaccctag aggtcccctt ttttatttta aaaatttttt cacaaaacgg tttacaagca
6301 taaagcttgc tcaatctgaa agcgaccagg tgctcggcgt ggcaagactc gcagcgaacc
6361 cgtagaaagc catgctccag ccgcccgcat tggagaaatt cttcaaattc ccgttgcaca
6421 tagcccggca attcctttcc ctgctctgcc ataagcgcag cgaatgccgg gtaatactcg
6481 tcaacgatct gatagagaag ggtttgctcg ggtcggtggc tctggtaacg accagtatcc
6541 cgatcccggc tggccgtcct ggccgccaca tgaggcatgt tccgcgtcct tgcaatactg
6601 tgtttacata cagtctatcg cttagcggaa agttctttta ccctcagccg aaatgcctgc
6661 cgttgctaga cattgccagc cagtgcccgt cactcccgta ctaactgtca cgaacccctg
6721 caataactgt cacgcccccc tgcaataact gtcacgaacc cctgcaataa ctgtcacgcc
6781 cccaaacctg caaacccagc aggggcgggg gctggcgggg tgttggaaaa atccatccat
6841 gattatctaa gaataatcca ctaggcgcgg ttatcagcgc ccttgtgggg cgctgctgcc
6901 cttgcccaat atgcccggcc agaggccgga tagctggtct attcgctgcg ctaggctaca
6961 caccgcccca ccgctgcgcg gcagggggaa aggcgggcaa agcccgctaa accccacacc
7021 aaaccccgca gaaatacgct ggagcgcttt tagccgcttt agcggccttt ccccctaccc
7081 gaagggtggg ggcgcgtgtg cagccccgca gggcctgtct cggtcgatca ttcagcccgg
7141 ctcatccttc tggcgtggcg gcagaccgaa caaggcgcgg tcgtggtcgc gttcaaggta
7201 cgcatccatt gccgccatga gccgatcctc cggccactcg ctgctgttca ccttggccaa
7261 aatcatggcc cccaccagca ccttgcgcct tgtttcgttc ttgcgctctt gctgctgttc
7321 ccttgcccgc acccgctgaa tttcggcatt gattcgcgct cgttgttctt cgagcttggc
7381 cagccgatcc gccgccttgt tgctcccctt aaccatcttg acaccccatt gttaatgtgc
7441 tgtctcgtag gctatcatgg aggcacagcg gcggcaatcc cgaccctact ttgtagggga
7501 gggcgcactt accggtttct cttcgagaaa ctggcctaac ggccaccctt cgggcggtgc
7561 gctctccgag ggccattgca tggagccgaa aagcaaaagc aacagcgagg cagcatggcg
7621 atttatcacc ttacggcgaa aaccggcagc aggtcgggcg gccaatcggc cagggccaag
7681 gccgactaca tccagcgcga aggcaagtat gcccgcgaca tggatgaagt cttgcacgcc
7741 gaatccgggc acatgccgga gttcgtcgag cggcccgccg actactggga tgctgccgac
7801 ctgtatgaac gcgccaatgg gcggctgttc aaggaggtcg aatttgccct gccggtcgag
7861 ctgaccctcg accagcagaa ggcgctggcg tccgagttcg cccagcacct gaccggtgcc
7921 gagcgcctgc cgtatacgct ggccatccat gccggtggcg gcgagaaccc gcactgccac
7981 ctgatgatct ccgagcggat caatgacggc atcgagcggc ccgccgctca gtggttcaag
8041 cggtacaacg gcaagacccc ggagaagggc ggggcacaga agaccgaagc gctcaagccc
8101 aaggcatggc ttgagcagac ccgcgaggca tgggccgacc atgccaaccg ggcattagag
8161 cgggctggcc acgacgcccg cattgaccac agaacacttg aggcgcaggg catcgagcgc
8221 ctgcccggtg ttcacctggg gccgaacgtg gtggagatgg aaggccgggg catccgcacc
8281 gaccgggcag acgtggccct gaacatcgac accgccaacg cccagatcat cgacttacag
8341 gaataccggg aggcaataga ccatgaacgc aatcgacaga gtgaagaaat ccagaggcat
8401 caacgagtta gcggagcaga tcgaaccgct ggcccagagc atggcgacac tggccgacga
8461 agcccggcag gtcatgagcc agacccagca ggccagcgag gcgcaggcgg cggagtggct
8521 gaaagcccag cgccagacag gggcggcatg ggtggagctg gccaaagagt tgcgggaggt
8581 agccgccgag gtgagcagcg ccgcgcagag cgcccggagc gcgtcgcggg ggtggcactg
8641 gaagctatgg ctaaccgtga tgctggcttc catgatgcct acggtggtgc tgctgatcgc
8701 atcgttgctc ttgctcgacc tgacgccact gacaaccgag gacggctcga tctggctgcg
8761 cttggtggcc cgatgaagaa cgacaggact ttgcaggcca taggccgaca gctcaaggcc
8821 atgggctgtg agcgcttcga tatcggcgtc agggacgcca ccaccggcca gatgatgaac
8881 cgggaatggt cagccgccga agtgctccag aacacgccat ggctcaagcg gatgaatgcc
8941 cagggcaatg acgtgtatat caggcccgcc gagcaggagc ggcatggtct ggtgctggtg
9001 gacgacctca gcgagtttga cttggatgac atgaaagccg agggccggga gcctgccctg
9061 gtagtggaaa ccagcccgaa gaactatcag gcatgggtca aggtggccga cgccgcaggc
9121 ggtgaacttc gggggcagat tgcccggacg ctggccagcg agtacgacgc cgacccggcc
9181 agcgccgaca gccgccacta tggccgcttg gcgggcttca ccaaccgcaa ggacaagcac
9241 accacccgcg ccggttatca gccgtgggtg ctgctgcgtg aatccaaggg caagaccgcc
9301 accgctggcc cggcgctggt gcagcaggct ggccagcaga tcgagcaggc ccagcggcag
9361 caggagaagg cccgcaggct ggccagcctc gaactgcccg agcggcagct tagccgccac
9421 cggcgcacgg cgctggacga gtaccgcagc gagatggccg ggctggtcaa gcgcttcggt
9481 gatgacctca gcaagtgcga ctttatcgcc gcgcagaagc tggccagccg gggccgcagt
9541 gccgaggaaa tcggcaaggc catggccgag gccagcccag cgctggcaga gcgcaagccc
9601 ggccacgaag cggattacat cgagcgcacc gtcagcaagg tcatgggtct gcccagcgtc
9661 cagcttgcgc gggccgagct ggcacgggca ccggcacccc gccagcgagg catggacagg
9721 ggcgggccag atttcagcat gtagtgcttg cgttggtact cacgcctgtt atactatgag
9781 tactcacgca cagaaggggg ttttatggaa tacgaaaaaa gcgcttcagg gtcggtctac
9841 ctgatcaaaa gtgacaaggg ctattggttg cccggtggct ttggttatac gtcaaacaag
9901 gccgaggctg gccgcttttc agtcgctgat atggccagcc ttaaccttga cggctgcacc
9961 ttgtccttgt tccgcgaaga caagcctttc ggccccggca agtttctcgg tgactgatat
10021 gaaagaccaa aaggacaagc agaccggcga cctgctggcc agccctgacg ctgtacgcca
10081 agcgcgatat gccgagcgca tgaaggccaa agggatgcgt cagcgcaagt tctggctgac
10141 cgacgacgaa tacgaggcgc tgcgcgagtg cctggaagaa ctcagagcgg cgcagggcgg
10201 gggtagtgac cccgccagcg cctaaccacc aactgcctgc aaaggaggca atcaatggct
10261 acccataagc ctatcaatat tctggaggcg ttcgcagcag cgccgccacc gctggactac
10321 gttttgccca acatggtggc cggtacggtc ggggcgctgg tgtcgcccgg tggtgccggt
10381 aaatccatgc tggccctgca actggccgca cagattgcag gcgggccgga tctgctggag
10441 gtgggcgaac tgcccaccgg cccggtgatc tacctgcccg ccgaagaccc gcccaccgcc
10501 attcatcacc gcctgcacgc ccttggggcg cacctcagcg ccgaggaacg gcaagccgtg
10561 gctgacggcc tgctgatcca gccgctgatc ggcagcctgc ccaacatcat ggccccggag
10621 tggttcgacg gcctcaagcg cgccgccgag ggccgccgcc tgatggtgct ggacacgctg
10681 cgccggttcc acatcgagga agaaaacgcc agcggcccca tggcccaggt catcggtcgc
10741 atggaggcca tcgccgccga taccgggtgc tctatcgtgt tcctgcacca tgccagcaag
10801 ggcgcggcca tgatgggcgc aggcgaccag cagcaggcca gccggggcag ctcggtactg
10861 gtcgataaca tccgctggca gtcctacctg tcgagcatga ccagcgccga ggccgaggaa
10921 tggggtgtgg acgacgacca gcgccggttc ttcgtccgct tcggtgtgag caaggccaac
10981 tatggcgcac cgttcgctga tcggtggttc aggcggcatg acggcggggt gctcaagccc
11041 gccgtgctgg agaggcagcg caagagcaag ggggtgcccc gtggtgaagc ctaagaacaa
11101 gcacagcctc agccacgtcc ggcacgaccc ggcgcactgt ctggcccccg gcctgttccg
11161 tgccctcaag cggggcgagc gcaagcgcag caagctggac gtgacgtatg actacggcga
11221 cggcaagcgg atcgagttca gcggcccgga gccgctgggc gctgatgatc tgcgcatcct
11281 gcaagggctg gtggccatgg ctgggcctaa tggcctagtg cttggcccgg aacccaagac
11341 cgaaggcgga cggcagctcc ggctgttcct ggaacccaag tgggaggccg tcaccgctga
11401 tgccatggtg gtcaaaggta gctatcgggc gctggcaaag gaaatcgggg cagaggtcga
11461 tagtggtggg gcgctcaagc acatacagga ctgcatcgag cgcctttgga aggtatccat
11521 catcgcccag aatggccgca agcggcaggg gtttcggctg ctgtcggagt acgccagcga
11581 cgaggcggac gggcgcctgt acgtggccct gaaccccttg atcgcgcagg ccgtcatggg
11641 tggcggccag catgtgcgca tcagcatgga cgaggtgcgg gcgctggaca gcgaaaccgc
11701 ccgcctgctg caccagcggc tgtgtggctg gatcgacccc ggcaaaaccg gcaaggcttc
11761 catagatacc ttgtgcggct atgtctggcc gtcagaggcc agtggttcga ccatgcgcaa
11821 gcgccgccag cgggtgcgcg aggcgttgcc ggagctggtc gcgctgggct ggacggtaac
11881 cgagttcgcg gcgggcaagt acgacatcac ccggcccaag gcggcaggct gacccccccc
11941 actctattgt aaacaagaca tttttatctt ttatattcaa tggcttattt tcctgctaat
12001 tggtaatacc atgaaaaata ccatgctcag aaaaggctta acaatatttt gaaaaattgc
12061 ctactgagcg ctgccgcaca gctccatagg ccgctttcct ggctttgctt ccagatgtat
12121 gctcttctgc tcctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
12181 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
12241 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
12301 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
12361 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
12421 cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca
12481 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
12541 gttcgatgta acccactcgt gcactttaca ctttatgctt ccggctcgta tgtta
//
