| 质粒名称: | At2S3:RUBY |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 160906 |
| 存储实验室: | Yunde Zhao |
|
载体骨架基本信息 |
|
| 载体名称: | pHDE |
| 载体出品公司: | Clontech |
| 空载体大小: | 9346bp |
| 完整质粒大小: | 13986bp |
| 载体类型: | 植物表达 |
| 筛选标记: | Spectinomycin |
| 载体抗性: | Spectinomycin |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | RUBY and At2S3 promoter |
| 物种来源: | Synthetic; Arabidopsis |
| 插入大小: | 4640bp |
| 启动子: | At2S3 |
| 克隆方法: | Gibson Cloning |
| 5’测序引物 | CTGTCAAACACTGATAGTTTtaagctggcacaactatatttcc |
| 3’测序引物 | GCTTACTCAGTTAGGTCTAGCTTATCTTTAATCATATTCCATAGTCCA |
|
文献引用方法 |
|
| 材料和方法部分: | At2S3:RUBY was a gift from Yunde Zhao (Addgene plasmid # 160906 ; http://n2t.net/addgene:160906 ; RRID:Addgene_160906) |
| 参考文献部分: | A reporter for noninvasively monitoring gene expression and plant transformation. He Y, Zhang T, Sun H, Zhan H, Zhao Y. Hortic Res. 2020 Sep 19;7:152. doi: 10.1038/s41438-020-00390-1. eCollection 2020. 10.1038/s41438-020-00390-1 PubMed 33024566 |
买家导航
At2S3:RUBY载体质粒基本信息
At2S3:RUBY质粒图谱载体图谱和At2S3:RUBY载体序列质粒序列多克隆位点信息
At2S3:RUBY质粒载体简介
At2S3:RUBY质粒序列载体序列
LOCUS At2S3:RUBY 14085 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION RUBY under the control of At2S3 promoter/marker for Arabidopsis
transgenes.
ACCESSION .
VERSION .
KEYWORDS At2S3:RUBY
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 14085)
AUTHORS He Y, Zhang T, Sun H, Zhan H, Zhao Y
TITLE A reporter for noninvasively monitoring gene expression and plant
transformation.
JOURNAL Hortic Res. 2020 Sep 19;7:152. doi: 10.1038/s41438-020-00390-1.
eCollection 2020.
PUBMED 33024566
REFERENCE 2 (bases 1 to 14085)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..14085
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 178..202
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 2152..2208
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 3043..3099
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
promoter 5306..5485
/label=NOS promoter
/note="nopaline synthase promoter"
promoter 5338..5521
/function="NOS promoter"
/label=NOS promoter
/note="nopaline synthase promoter"
terminator 6636..6888
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
primer_bind complement(7463..7480)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(7463..7479)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7472..7494)
/label=M13/pUC Forward
/note="In lacZ gene"
misc_feature 7701..7725
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
CDS 8246..9037
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
rep_origin 9283..9871
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 9772..9791
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 10025..10042
/label=L4440
/note="L4440 vector, forward primer"
misc_feature 10057..10197
/label=bom
/note="basis of mobility region from pBR322"
primer_bind complement(10123..10142)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 10283..10305
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
rep_origin 10541..10735
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
CDS complement(10801..11874)
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
CDS complement(12303..12932)
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
1 actatagcag cggaggggtt ggatcaaagt actttaaagt actttaaagt actttaaagt
61 actttgatcc cgaggggaac cctgtggttg gcatgcacat acaaatggac gaacggataa
121 accttttcac gcccttttaa atatccgatt attctaataa acgctctttt ctcttaggtt
181 tacccgccaa tatatcctgt caaacactga tagttttaag ctggcacaac tatatttcca
241 acatcactag ctaccatcaa aagattgact tctcatctta ctcgattgaa accaaattaa
301 catagggttt ttatttaaat aaaagtttaa ccttcttttt aaaaaattgt tcatagtgtc
361 atgtcagaac aagagctaca aatcacacat agcatgcata agcggagcta tgatgagtgg
421 tattgttttg ttcgtcactt gtcactcttt tccaacacat aatcccgaca acaacgtaag
481 agcatctctc tctctccaca cacactcatg catgcatgca ttcttacacg tgattgccat
541 gcaaatctcc tttctcacct ataaatacaa accaaccctt cactacactc ttcactcaaa
601 ccaaaacaag aaaacataca caaatagcaa aacggtacca acaaaactag tatggatcat
661 gcgaccctcg ccatgatcct cgcgatctgg ttcatcagct tccacttcat caagctgctg
721 ttctcccagc agaccaccaa gctgcttccg ccaggaccaa agccgcttcc gatcatcggc
781 aacatccttg aggtgggcaa gaagccgcat cggtccttcg ccaacctcgc caagattcac
841 ggcccactca tttccctcag actcggctct gtgaccacca tcgttgtgtc ctctgccgac
901 gtggccaaag agatgttcct caagaaggat cacccgctct ccaaccgcac gatcccgaat
961 agtgttacag ccggcgacca ccacaagctc accatgtctt ggctcccggt gtctccgaag
1021 tggcgcaact tccgcaagat taccgccgtg catctgctct ccccacagag actcgatgcc
1081 tgccagacat tcaggcacgc caaggtgcag cagctctacg agtacgttca agagtgcgcc
1141 cagaaaggcc aggccgtgga tattggcaag gccgccttta cgaccagcct caacctcctc
1201 agcaagctgt tcttcagcgt cgagctggcg caccacaagt cccataccag ccaagagttc
1261 aaagagctga tctggaacat catggaagat ataggcaagc cgaactacgc cgactacttc
1321 ccgattctcg gctgcgttga cccatctggc attagaagaa ggctcgcctg ctccttcgac
1381 aagctgatcg ccgtgttcca gggcatcatc tgcgagagac tcgccccaga ttcctccacc
1441 acaactacca ccaccaccga cgacgtgctc gatgtgctcc tccagctgtt caagcagaac
1501 gagctgacga tgggcgagat caaccacctc ctcgtggaca tcttcgacgc cggcaccgat
1561 accacatcct ccacattcga gtgggtgatg accgagctga tccgcaatcc agagatgatg
1621 gaaaaggccc aagaggaaat caagcaggtc ctcggcaagg acaagcagat ccaagagtcc
1681 gacatcatca acctgccgta cctccaggcg atcatcaaag agacactccg cctccatccg
1741 ccgaccgtgt tcttgctccc aagaaaggcc gacaccgatg tcgagctgta cggctacatc
1801 gtgccgaagg atgcccagat cctcgtgaac ctctgggcca ttggcaggga cccaaacgcc
1861 tggcagaacg ccgatatttt cagcccagag cgcttcatcg gctgcgagat cgatgttaag
1921 ggccgcgatt tcggcctcct tccatttggc gctggccgca gaatttgccc aggcatgaat
1981 ctcgccatca ggatgctcac cctcatgctc gccacactcc tccagttctt caactggaag
2041 ctcgaaggcg acatctcccc gaaggacctc gacatggacg agaagttcgg cattgcgctc
2101 caaaagacca agccgctcaa gctcatcccg attccgcgct acggtagcgg agctaccaat
2161 tttagcctcc ttaagcaggc aggtgatgta gaagagaacc ccgggcctat gaagatgatg
2221 aacggcgagg acgccaacga ccagatgatc aaagagtcct tcttcatcac ccacggcaac
2281 ccgatcctca ccgtcgagga tacacatccg ctcaggccgt tcttcgagac atggcgcgag
2341 aagattttct ccaagaagcc gaaggccatc ctcatcatct ccggccactg ggagacagtg
2401 aagccaaccg tgaacgccgt gcacatcaac gacaccatcc acgacttcga cgactaccca
2461 gccgccatgt accagttcaa gtacccagct ccaggcgagc cagagcttgc gagaaaggtg
2521 gaagagatcc tcaagaagtc cgggttcgag acagccgaga cagaccaaaa gaggggcctt
2581 gatcacggcg cctgggttcc actcatgctc atgtatccag aggcggacat cccggtgtgc
2641 cagctctcag ttcagccaca tctcgacggc acctaccact acaatctcgg cagagccctc
2701 gcgccgctca agaatgatgg cgtgctcatt attggctccg gcagcgccac acatccactc
2761 gatgagacac cgcactactt cgatggtgtt gccccttggg ccgctgcctt cgattcttgg
2821 cttaggaagg ccctcatcaa cggccgcttc gaggaagtga acatctacga gagcaaggcc
2881 ccgaactgga agctcgccca tccatttcca gagcacttct acccgctcca cgttgtgctc
2941 ggcgctgctg gtgaaaagtg gaaggccgag ctgatccact cctcctggga tcatggcaca
3001 ctttgccacg gctcctacaa gttcacctcc gccggatccg gagcaaccaa ctttagcctg
3061 ctcaagcaag caggagatgt tgaggaaaat cctggcccca tgaccgccat caagatgaac
3121 accaacggcg agggcgagac acagcacatc ctcatgatcc cgttcatggc gcagggccac
3181 ctcaggccat ttctcgaact cgccatgttc ctctacaagc gctcccacgt gatcatcacc
3241 ctgctcacaa ctccgctcaa tgccggcttc ctcaggcacc tccttcacca ccattcctac
3301 tcctccagcg gcatcaggat cgtcgagctg ccattcaact ccaccaacca cggactccca
3361 ccgggcatcg agaacaccga taagctcaca ctcccgctcg tggtgtccct cttccattcc
3421 accatcagcc tcgatccgca cctccgcgat tacatctcca ggcatttcag cccagccagg
3481 ccaccactct gcgtgatcca tgatgtgttc ctcggctggg ttgaccaggt ggccaaggat
3541 gtgggctcta caggcgtggt gttcacaaca ggcggcgctt atggcacatc cgcctacgtg
3601 tccatctgga acgatctccc gcaccagaac tactccgacg accaagagtt cccgctgcca
3661 ggcttcccag agaaccataa gttccgcagg tcccagctcc atcggttcct cagatatgcc
3721 gacggctccg acgattggtc caagtatttc cagccgcagc tccgccagtc catgaagtct
3781 tttggctggc tctgcaactc cgtggaagag atcgagacac tcggcttctc catcctccgc
3841 aactacacca agctgccgat ctggggcatc ggcccactta ttgcttcccc agtgcagcac
3901 tcctcctccg acaacaattc aacaggcgcc gagttcgtgc agtggctcag cctcaaagag
3961 ccggactccg tcctctacat ctccttcggc tcccagaaca cgatcagccc gacgcagatg
4021 atggaactcg ctgctggcct tgagtcctcc gagaagccat tcctctgggt gatcagagcc
4081 ccgttcggct tcgacatcaa cgaagagatg cgcccagagt ggctgccaga gggctttgag
4141 gaacgcatga aggtgaagaa acagggcaag ctcgtgtaca agctcggccc gcagcttgag
4201 atcctcaacc atgaatccat cggcggcttt ctcacccact gcggatggaa cagcatcctt
4261 gagtctcttc gcgagggcgt tccgatgctt ggatggccac ttgctgccga gcaggcctac
4321 aacctcaagt acctcgaaga tgagatgggc gtcgcggttg agcttgctag aggcctcgaa
4381 ggcgagatct ccaaagagaa ggtcaagcgc atcgtcgaga tgatccttga gcgcaacgag
4441 ggctccaaag gctgggagat gaagaatcgc gccgtggaaa tgggcaaaaa gctcaaggac
4501 gccgtgaacg aggaaaaaga gctgaagggc tcctccgtga aggcgatcga cgatttcctc
4561 gacgccgtca tgcaggccaa acttgagcca agcctccagt gatagtgaat atgaagatga
4621 agatgaaata tttggtgtgt caaataaaaa ggttgtgtgc ttaagtttgt gtttttttct
4681 tggcttgttg tgttatgaat ttgtggcttt ttctaatatt aaatgaatgt aacatctcat
4741 tataatgaat aaacaaatgt ttctataatc cattgtgaat gttttgttgg atctcttctc
4801 cagcatataa ctactgtatg tgctatggta tggactatgg aatatgatta aagataagac
4861 ctaactgagt aagctagctt gttcgagtat tatggcattg ggaaaactgt ttttcttgta
4921 ccatttgttg tgcttgtaat ttactgtgtt ttttattcgg ttttcgctat cgaactgtga
4981 aatggaaatg gatggagaag agttaatgaa tgatatggtc cttttgttca ttctcaaatt
5041 aatattattt gttttttctc ttatttgttg tgtgttgaat ttgaaattat aagagatatg
5101 caaacatttt gttttgagta aaaatgtgtc aaatcgtggc ctctaatgac cgaagttaat
5161 atgaggagta aaacactaga tccccaaaca agcttgaaac tgaaggcggg aaacgacaat
5221 ctgatcatga gcggagaatt aagggagtca cgttatgacc cccgccgatg acgcgggaca
5281 agccgtttta cgtttggaac tgacagaacc gcaacgttga aggagccact cagccgcggg
5341 tttctggagt ttaatgagct aagcacatac gtcagaaacc attattgcgc gttcaaaagt
5401 cgcctaaggt cactatcagc tagcaaatat ttcttgtcaa aaatgctcca ctgacgttcc
5461 ataaattccc ctcggtatcc aattagagtc tcatattcac tctcaatcca aataatctgc
5521 accggatccg ctagaggatc tcgacctgca agatcccggg gggcaatgag atatgaaaaa
5581 gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc
5641 cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atataggagg
5701 gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt
5761 ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggaatt
5821 cagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct
5881 gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc
5941 tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca
6001 atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca
6061 aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct
6121 ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa
6181 tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg
6241 ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga
6301 gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc cgcggctccg
6361 ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt
6421 cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac
6481 tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga
6541 agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgggatctt ggaggtgatg
6601 taacatgatc acaagctgat cccccgaatt tccccgatcg ttcaaacatt tggcaataaa
6661 gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga
6721 attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt
6781 ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg
6841 caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg gaattgatcc
6901 cccctcgaca gcttgcatgc cagcttgggc tgcaggtcga cgcgtcaatt gatatcgatc
6961 caggcctccc agctttcgtc cgtatcatcg gtttcgacaa cgttcgtcaa gttcaatgca
7021 tcagtttcat tgcccacaca ccagaatcct actaagtttg agtattatgg cattggaaaa
7081 gctgttttct tctatcattt gttctgcttg taatttactg tgttctttca gtttttgttt
7141 tcggacatca aaatgcaaat ggatggataa gagttaataa atgatatggt ccttttgttc
7201 attctcaaat tattattatc tgttgttttt actttaatgg gttgaattta agtaagaaag
7261 gaactaacag tgtgatatta aggtgcaatg ttagacatat aaaacagtct ttcacctctc
7321 tttggttatg tcttgaattg gtttgtttct tcacttatct gtgtaatcaa gtttactatg
7381 agtctatgat caagtaatta tgcaatcaag ttaagtacag tataggcttt ttgtgtcgag
7441 ggggtaccga gtcgaggaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
7501 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
7561 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgggtacc
7621 gagctcgaat tcaattcggc gttaattcag tacattaaaa acgtccgcaa tgtgttatta
7681 agttgtctaa gcgtcaattt gtttacacca caatatatcc tgccaccagc cagccaacag
7741 ctccccgacc ggcagctcgg cacaaaatca ccactcgata caggcagccc atcagtccgg
7801 gacggcgtca gcgggagagc cgttgtaagg cggcagactt tgctcatgtt accgatgcta
7861 ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg gatgatctcg cggagggtag
7921 catgttgatt gtaacgatga cagagcgttg ctgcctgtga tcaattcggg cacgaaccca
7981 gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa
8041 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc
8101 ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg
8161 ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag
8221 tcgccctaaa acaaagttaa acatcatggg ggaagcggtg atcgccgaag tatcgactca
8281 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
8341 tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt
8401 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
8461 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
8521 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
8581 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
8641 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
8701 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
8761 atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg
8821 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
8881 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct
8941 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta
9001 cgtgaaaggc gagatcacca aggtagtcgg caaataatgt ctagctagaa attcgttcaa
9061 gccgacgccg cttcgcggcg cggcttaact caagcgttag atgcactaag cacataattg
9121 ctcacagcca aactatcagg tcaagtctgc ttttattatt tttaagcgtg cataataagc
9181 cctacacaaa ttgggagata tatcatgcat gaccaaaatc ccttaacgtg agttttcgtt
9241 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
9301 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
9361 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
9421 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
9481 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
9541 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
9601 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
9661 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
9721 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
9781 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
9841 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
9901 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
9961 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
10021 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac
10081 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
10141 cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc
10201 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
10261 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
10321 ccgaaacgcg cgaggcaggg tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc
10381 ggcttgtccg cgccctggta gattgcctgg ccgtaggcca gccatttttg agcggccagc
10441 ggccgcgata ggccgacgcg aagcggcggg gcgtagggag cgcagcgacc gaagggtagg
10501 cgctttttgc agctcttcgg ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt
10561 tttaagagtt ttaataagtt ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt
10621 tatatcagtc acttacatgt gtgaccggtt cccaatgtac ggctttgggt tcccaatgta
10681 cgggttccgg ttcccaatgt acggctttgg gttcccaatg tacgtgctat ccacaggaaa
10741 gagacctttt cgaccttttt cccctgctag ggcaatttgc cctagcatct gctccgtaca
10801 ttaggaaccg gcggatgctt cgccctcgat caggttgcgg tagcgcatga ctaggatcgg
10861 gccagcctgc cccgcctcct ccttcaaatc gtactccggc aggtcatttg acccgatcag
10921 cttgcgcacg gtgaaacaga acttcttgaa ctctccggcg ctgccactgc gttcgtagat
10981 cgtcttgaac aaccatctgg cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag
11041 aaaacggccg atgccgggat cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg
11101 gttcttgcct tctgtgatct cgcggtacat ccaatcagct agctcgatct cgatgtactc
11161 cggccgcccg gtttcgctct ttacgatctt gtagcggcta atcaaggctt caccctcgga
11221 taccgtcacc aggcggccgt tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt
11281 gtttaaccga atgcaggttt ctaccaggtc gtctttctgc tttccgccat cggctcgccg
11341 gcagaacttg agtacgtccg caacgtgtgg acggaacacg cggccgggct tgtctccctt
11401 cccttcccgg tatcggttca tggattcggt tagatgggaa accgccatca gtaccaggtc
11461 gtaatcccac acactggcca tgccggccgg ccctgcggaa acctctacgt gcccgtctgg
11521 aagctcgtag cggatcacct cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc
11581 cacgtccatg atgctgcgac tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa
11641 atctggttgc tcgtcgccct tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg
11701 ttgccgggat tctttgcgga ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc
11761 ttctgcctcg atgcgttgcc gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc
11821 atcacccagc gccgcgccga tttgtaccgg gccggatggt ttgcgaccgc tcacgccgat
11881 tcctcgggct tgggggttcc agtgccattg cagggccggc agacaaccca gccgcttacg
11941 cctggccaac cgcccgttcc tccacacatg gggcattcca cggcgtcggt gcctggttgt
12001 tcttgatttt ccatgccgcc tcctttagcc gctaaaattc atctactcat ttattcattt
12061 gctcatttac tctggtagct gcgcgatgta ttcagatagc agctcggtaa tggtcttgcc
12121 ttggcgtacc gcgtacatct tcagcttggt gtgatcctcc gccggcaact gaaagttgac
12181 ccgcttcatg gctggcgtgt ctgccaggct ggccaacgtt gcagccttgc tgctgcgtgc
12241 gctcggacgg ccggcactta gcgtgtttgt gcttttgctc attttctctt tacctcatta
12301 actcaaatga gttttgattt aatttcagcg gccagcgcct ggacctcgcg ggcagcgtcg
12361 ccctcgggtt ctgattcaag aacggttgtg ccggcggcgg cagtgcctgg gtagctcacg
12421 cgctgcgtga tacgggactc aagaatgggc agctcgtacc cggccagcgc ctcggcaacc
12481 tcaccgccga tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt
12541 ccatccgtga cctcaatgcg ctgcttaacc agctccacca ggtcggcggt ggcccatatg
12601 tcgtaagggc ttggctgcac cggaatcagc acgaagtcgg ctgccttgat cgcggacaca
12661 gccaagtccg ccgcctgggg cgctccgtcg atcactacga agtcgcgccg gccgatggcc
12721 ttcacgtcgc ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc
12781 cgcacggccg cccaatcgcg ggcactgccc tggggatcgg aatcgactaa cagaacatcg
12841 gccccggcga gttgcagggc gcgggctaga tgggttgcga tggtcgtctt gcctgacccg
12901 cctttctggt taagtacagc gataaccttc atgcgttccc cttgcgtatt tgtttattta
12961 ctcatcgcat catatacgca gcgaccgcat gacgcaagct gttttactca aatacacatc
13021 acctttttag acggcggcgc tcggtttctt cagcggccaa gctggccggc caggccgcca
13081 gcttggcatc agacaaaccg gccaggattt catgcagccg cacggttgag acgtgcgcgg
13141 gcggctcgaa cacgtacccg gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa
13201 acggttcgtc ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc gttcattctc
13261 ggcggccgcc agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct
13321 ggcctcggtg ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg
13381 cacgccaagc agtgcagccg cctctttcac ggtgcggcct tcctggtcga tcagctcgcg
13441 ggcgtgcgcg atctgtgccg gggtgagggt agggcggggg ccaaacttca cgcctcgggc
13501 cttggcggcc tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct cgaactcggc
13561 aatgccggcg aacacggtca acaccatgcg gccggccggc gtggtggtgt cggcccacgg
13621 ctctgccagg ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag
13681 gtcgcgggtg ctgcgggcca ggcggtctag cctggtcact gtcacaacgt cgccagggcg
13741 taggtggtca agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc
13801 ggaaaacagc ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg
13861 gtcgtcggtg ctgacgcggg catagcccag caggccagcg gcggcgctct tgttcatggc
13921 gtaatgtctc cggttctagt cgcaagtatt ctactttatg cgactaaaac acgcgacaag
13981 aaaacgccag gaaaagggca gggcggcagc ctgtcgcgta acttaggact tgtgcgacat
14041 gtcgttttca gaagacggct gcactgaacg tcagaagccg actgc
//
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