| 出品公司: | EMD Biosciences (Novagen) |
|---|---|
| 别名: | pET40b, pET 40b |
| 质粒类型: | 大肠杆菌蛋白表达 |
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 6190 bp |
| 5' 测序引物: | T7 |
| 5' 测序引物序列: | T7: 5'-TAATACGACTCACTATAGGG-3' |
| 载体标签: | His (中间和C端), N-DsbC, N-S, N-EK, N-Thrombin |
| 载体抗性: | Kanamycin |
| 备注: |
Encodes DsbC tag for export and periplasmic folding of target proteins.
|
| 表达宿主菌: | BL21(DE3) 、 Rosetta2(DE3)、C43(DE3) |
| 产品目录号: | 70091-3 |
| 稳定性: | 瞬时表达 Transient |
| 组成型: | 组成型 Constitutive |
| 病毒/非病毒: | 非病毒 |
| 表达宿主菌: | BL21(DE3) 、BLR(DE3)、Rosetta(DE3)、OrigamiB(DE3) |
| pET表达手册： |
pET表达手册英文版 pET表达手册中文版 |
| pET载体汇总: |
pET-3a pET-11a pET-12a pET-14b pET-15b pET-16b pET-17b pET-19b pET-20b pET-21a pET-22b pET-23a pET-24a pET-25b pET-26b pET-27b pET-28a pET-29a pET-30a pET-31b pET-32a pET-33b pET-34b pET-35b pET-37b pET-39b pET-40b pET-41a pET-42a pET-43.1a pET-44a pET-45b pET-47b pET-48b pET-49b pET-50b pET-51b pET-52b pETDuet-1 pACYCDuet-1 pCDFDuet-1 pRSFDuet-1 pColaDuet-1 |
买家导航
pET-40b(+)载体 pET40b质粒基本信息
pET-40b(+)质粒图谱 pET40b载体图谱和pET-40b(+)载体序列 pET40b质粒序列多克隆位点信息
| Feature Name | Start | End | |
|---|---|---|---|
| T7_terminator | 120 | 1 | |
| T7_Terminal_primer | 69 | 87 | |
| EK | 266 | 252 | |
| S15 | 326 | 282 | |
| 6xHIS | 386 | 369 | |
| T7_transl_en_RBS | 1140 | 1124 | |
| lacO | 1185 | 1158 | |
| T7_promoter | 1203 | 1185 | |
| tet (300 - 563) | 1239 | 1502 | |
| pBRrevBam_primer | 1310 | 1291 | |
| lacI | 1585 | 2676 | |
| tet (576 - 851) | 2735 | 3010 | |
| ROP | 3485 | 3676 | |
| pGEX_3_primer | 3692 | 3670 | |
| pBR322_origin | 4710 | 4091 | |
| KanR2 | 4816 | 5631 |
| ORF | Start | End | |
|---|---|---|---|
| ORF frame 3 | 1115 | 147 | |
| ORF frame 3 | 1116 | 1844 | |
| ORF frame 1 | 1717 | 2676 | |
| ORF frame 1 | 4816 | 5631 |
| Enzyme Name | Cut |
|---|---|
| XhoI | 174 |
| NotI | 182 |
| EagI | 182 |
| HindIII | 189 |
| SalI | 195 |
| SacI | 206 |
| EcoRI | 208 |
| BamHI | 214 |
| EcoRV | 222 |
| NcoI | 227 |
| KpnI | 271 |
| BglII | 274 |
| BstBI | 301 |
| SacII | 365 |
| SpeI | 396 |
| NdeI | 1114 |
| XbaI | 1152 |
| BclI | 1958 |
| ApaI | 2155 |
| HpaI | 2450 |
| FspI | 3026 |
| NruI | 4904 |
pET-40b(+)质粒 pET40b载体简介
pET-40b载体是带有Dsbs融合蛋白标签。本载体带有N-Trx标签和His标签，S标签。独特的酶切位点在载体图谱中被标注出来。需要注意的是，这些载体序列是依据载体pBR322的方向来进行编号的，所以T7标签表达区域在载体图谱上是反向的。
pET-40b(+)质粒序列 pET40b载体序列
LOCUS pET-40b(+) 6190 bp DNA Circular SYN
DEFINITION pET-40b(+)
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTOAUTHORAD1|Novagen Inc.|
FEATURES Location/Qualifiers
source 1..6190
/organism="pET-40b(+)"
/mol_type="other DNA"
terminator complement(1..120)
/label="T7_terminator"
misc_feature 69..87
/label="T7_Terminal_primer"
CDS complement(147..1115)
/label="ORF frame 3"
/translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
PKEMKEFLDEHQKMTSGKGSTSGSGHHHHHHSAGLVPRGSTAIGMKETAAAKFERQHM
DSPDLGTDDDDKSPGFSSTMAISDPNSSSVDKLAAALEHHHHHHHH*"
misc_feature complement(252..266)
/label="EK"
/translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
PKEMKEFLDEHQKMTSGKGSTSGSGHHHHHHSAGLVPRGSTAIGMKETAAAKFERQHM
DSPDLGTDDDDKSPGFSSTMAISDPNSSSVDKLAAALEHHHHHHHH*"
misc_feature complement(282..326)
/label="S15"
/translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
PKEMKEFLDEHQKMTSGKGSTSGSGHHHHHHSAGLVPRGSTAIGMKETAAAKFERQHM
DSPDLGTDDDDKSPGFSSTMAISDPNSSSVDKLAAALEHHHHHHHH*"
misc_feature complement(369..386)
/label="6xHIS"
/translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
PKEMKEFLDEHQKMTSGKGSTSGSGHHHHHHSAGLVPRGSTAIGMKETAAAKFERQHM
DSPDLGTDDDDKSPGFSSTMAISDPNSSSVDKLAAALEHHHHHHHH*"
CDS 1116..1844
/label="ORF frame 3"
/translation="MYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSISILYAGR
IVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHA
PFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGEC
ETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQ
TVVADWRCHLQSGPARAVANCRGD*"
misc_feature complement(1124..1140)
/label="T7_transl_en_RBS"
/translation="MYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSISILYAGR
IVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHA
PFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGEC
ETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQ
TVVADWRCHLQSGPARAVANCRGD*"
misc_feature complement(1158..1185)
/label="lacO"
/translation="MYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSISILYAGR
IVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHA
PFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGEC
ETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQ
TVVADWRCHLQSGPARAVANCRGD*"
promoter complement(1185..1203)
/label="T7_promoter"
/translation="MYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSISILYAGR
IVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHA
PFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGEC
ETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQ
TVVADWRCHLQSGPARAVANCRGD*"
gene 1239..1502
/label="tet (300 - 563)"
/gene="tet (300 - 563)"
/translation="MYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSISILYAGR
IVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHA
PFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGEC
ETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQ
TVVADWRCHLQSGPARAVANCRGD*"
misc_feature complement(1291..1310)
/label="pBRrevBam_primer"
/translation="MYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSISILYAGR
IVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHA
PFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGEC
ETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQ
TVVADWRCHLQSGPARAVANCRGD*"
misc_feature 1585..2676
/label="lacI"
/translation="MYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSISILYAGR
IVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHA
PFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGEC
ETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQ
TVVADWRCHLQSGPARAVANCRGD*"
CDS 1717..2676
/label="ORF frame 1"
/translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
gene 2735..3010
/label="tet (576 - 851)"
/gene="tet (576 - 851)"
/translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
misc_feature 3485..3676
/label="ROP"
/translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
misc_feature complement(3670..3692)
/label="pGEX_3_primer"
/translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
rep_origin complement(4091..4710)
/label="pBR322_origin"
/translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
gene 4816..5631
/label="KanR2"
/gene="KanR2"
/translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
CDS 4816..5631
/label="ORF frame 1"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
ORIGIN
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
121 tgtttagcag cctaggtatt aatcaattag tggtggtggt ggtggtggtg gtgctcgagt
181 gcggccgcaa gcttgtcgac ggagctcgaa ttcggatccg atatcgccat ggttgaggag
241 aagcccgggc tcttgtcgtc gtcatcggta cccagatctg ggctgtccat gtgctggcgt
301 tcgaatttag cagcagcggt ttctttcata ccaattgcag tactaccgcg tggcaccaga
361 cccgcggagt gatggtgatg gtgatgacca gaaccactag ttgatccttt accgctggtc
421 attttttggt gttcgtcgag aaattctttc atctctttcg gcggctggta acccggaaca
481 agtgtgccat tgctcagcac aactgccgga gtaccgctaa cgccaagctg gacgccaagt
541 gcgtaatggt cggcaatatc cacgtcgcaa ctggctggtg cgacgctttt acctgccatc
601 acatcatcaa acgctttgtt tttatctttc gcacaccaga tagctttcat ttctttctct
661 gcatcgctgt ccagcccctg gcgcgggaaa gcaagataac gcacggtgat ccccagcgcg
721 ttgtagtctg ccatttgctc atgcagtttg tggcagtaac cacaggtaat atcagtaaac
781 acggtgatga cgtgtttttc ctgcggcgct ttataaacga tcatctcttt ttcaagcgca
841 ttcaactgct ttaacagcat cttattggtg acattgaccg gagccgtgcc actaacgtca
901 tacattggcc cctgaatgat atgtttacca tcatcggtga tgtacaacac gccgctgtta
961 gtcagaactg tcttcatgcc agctacaggc gcgggctgaa tatcgctgct tttgatgccc
1021 attttggcta acgtttgttg aattgccgcg tcatcagcct gagcaaagcc tgaaaacgcc
1081 gctaacaaag taaacaacat aaaacctttc ttcatatgta tatctccttc ttaaagttaa
1141 acaaaattat ttctagaggg gaattgttat ccgctcacaa ttcccctata gtgagtcgta
1201 ttaatttcgc gggatcgaga tcgatctcga tcctctacgc cggacgcatc gtggccggca
1261 tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag
1321 atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc
1381 ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg
1441 tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag
1501 agcgtcgaga tcccggacac catcgaatgg cgcaaaacct ttcgcggtat ggcatgatag
1561 cgcccggaag agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt atacgatgtc
1621 gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca ggccagccac
1681 gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa ttacattccc
1741 aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc
1801 agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa
1861 ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg
1921 gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta tccgctggat
1981 gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt atttcttgat
2041 gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg tacgcgactg
2101 ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc gggcccatta
2161 agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac tcgcaatcaa
2221 attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc
2281 atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa cgatcagatg
2341 gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc ggacatctcg
2401 gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtt aaccaccatc
2461 aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag
2521 ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc
2581 ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
2641 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtaagtta
2701 gctcactcat taggcaccgg gatctcgacc gatgcccttg agagccttca acccagtcag
2761 ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat
2821 catgcaactc gtaggacagg tgccggcagc gctctgggtc attttcggcg aggaccgctt
2881 tcgctggagc gcgacgatga tcggcctgtc gcttgcggta ttcggaatct tgcacgccct
2941 cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc aggccattat
3001 cgccggcatg gcggccccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct
3061 aggctggcgg ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg
3121 aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt
3181 ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat
3241 ctgcatcgca ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg
3301 ctggcattga ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt
3361 accctcacaa cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat
3421 cctctctcgt ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc
3481 atcagtgacc aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac
3541 attaacgctt ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga
3601 atcgcttcac gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga
3661 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
3721 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc
3781 agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca
3841 gagcagattg tactgagagt gcaccatata tgcggtgtga aataccgcac agatgcgtaa
3901 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
3961 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
4021 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
4081 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
4141 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
4201 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
4261 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
4321 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
4381 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
4441 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
4501 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
4561 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
4621 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
4681 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
4741 aaaactcacg ttaagggatt ttggtcatga acaataaaac tgtctgctta cataaacagt
4801 aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cttgctctag gccgcgatta
4861 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
4921 tcaggtgcga caatctatcg attgtatggg aagcccgatg cgccagagtt gtttctgaaa
4981 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg
5041 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
5101 ttactcacca ctgcgatccc cgggaaaaca gcattccagg tattagaaga atatcctgat
5161 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
5221 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
5281 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
5341 gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact
5401 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
5461 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
5521 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
5581 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta agaattaatt
5641 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
5701 atttccccga aaagtgccac ctgaaattgt aaacgttaat attttgttaa aattcgcgtt
5761 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
5821 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
5881 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
5941 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
6001 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
6061 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
6121 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
6181 ccattcgcca
//
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pET-40b(+)质粒 pET40b载体应用举例
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