| 出品公司: | NIH |
|---|---|
| 载体名称: |
pNL4-3-Luc-R-E-、pNL4.3-Luc pNL4-3-Luc、HIV-1 NL4-3 ΔEnv Vpr Luciferase Reporter Vector |
| 载体又名： |
HIV-1 NL4-3 ΔEnv Vpr Luciferase Reporter Vector 、 HIV-1 vector pNL4-3-Luc-R-E-、pNL4-3-Luc-R-E-载体、pNL4.3-Luc载体 HIV-1 NL4-3 ΔEnv Vpr Luciferase Reporter Vector (pNL4-3.Luc.R-E-) |
| 质粒类型: | 哺乳动物细胞载体；逆病毒包装载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 16396 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 存储人: | Dr. Nathaniel Landau, Aaron Diamond AIDS Research Center, The Rockefeller University. |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 嘌呤霉素（Puromycin） |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
| 备注: |
pNL4-3-Luc-R-E-载体，又叫pNL4-3-Luc载体，是一个HIV-1 NL4-3荧光素酶报告质粒，包含有缺陷的Nef, Env 和 Vpr. 这个质粒与含有env蛋白表达质粒共转染哺乳细胞，能够包装产生病毒，但是该病毒不具有再次扩增能力，因子病毒只能由共转染的质粒产生一轮病毒。 |
| 产品目录号: | 3418 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
| 生物危害性: | 无 |
| 参考文献: |
He J, Choe S, Walker R, Di Marzio P, Morgan DO, Landau NR. Human immunodeficiency virus type 1 viral protein R (Vpr) arrests cells in the G2 phase of the cell cycle by inhibiting p34cdc2 activity. J Virol 69: 6705–6711, 1995.
Connor RI, Chen BK, Choe S, Landau NR. Vpr is required for efficient replication of human immunodeficiency virus type-1 in mononuclear phagocytes. Virology 206: 935–944, 1995.
|
| 文章引用格式: |
文章引用部分可以这样书写：“The following reagent was obtained through the NIH AIDS Reagent Program, Division of AIDS, NIAID, NIH: pNL4-3.Luc.R–.E– from Dr. Nathaniel Landau.” Also include the references cited above in any publications. |
| 载体说明书: |
pNL4-3-Luc-R-E-载体说明书 pNL4-3-Luc-R-E-载体使用方法 pNL4-3-Luc-R-E-载体图谱和序列 |
| 相关载体: |
pNL4-3 NL4-3-ΔEnv-EGFP |
买家导航
pNL4.3-Luc HIV-1 NL4-3 ΔEnv Vpr Luciferase载体基本信息
pNL4.3-Luc HIV-1 NL4-3 ΔEnv Vpr Luciferase载体图谱质粒图谱和多克隆位点信息
pNL4.3-Luc HIV-1 NL4-3 ΔEnv Vpr Luciferase载体简介
pNL4.3-Luc HIV-1 NL4-3 ΔEnv Vpr Luciferase载体序列
LOCUS pNL4-3-Luc-R-E- 16396 bp ds-DNA circular SYN 26-MAR-2020
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 16396)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Thursday, March 26, 2020 from SnapGene Viewer 3.2.1 to
Vector NTI(R) format
http://www.biofeng.com
COMMENT VNTDATE|909100800|
COMMENT VNTDBDATE|909100800|
COMMENT VNTNAME|pNL4-3-luc|
FEATURES Location/Qualifiers
promoter 669..773
/gene="bla"
/label=AmpR promoter
CDS 774..1634
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
rep_origin 1805..2393
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
LTR 4204..4837
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
CDS complement(5027..6673)
/codon_start=1
/gene="luc"
/product="firefly luciferase"
/label=luciferase
misc_feature 7486..7719
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS complement(10388..13399)
/codon_start=1
/gene="pol"
/product="pol protein from human immunodeficiency virus 1"
/label=HIV-1 pol
misc_feature 10586..10703
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
CDS complement(13192..14694)
/codon_start=1
/gene="gag"
/product="gag protein from human immunodeficiency virus 1"
/label=HIV-1 gag
LTR 14850..15107
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
1 tgctggactg gcctagattt tgtatacaca catttcaggt gaaggtctaa aaaaaactgg
61 agcatataaa gaaaaataga atatatgtgt aattctggaa taaggaagga ctgtgtttcc
121 aaaatagaaa ataaataaat aaataaagga aaagattgga atatctgatt gatttattta
181 ttttattttt gagacagagt cttactctgt cgcccaggct ggagtgcagt gctgcgatct
241 cagcttactg caatctctgc ctcccctggc gtaatagcga agaggcccgc accgatcgcc
301 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta
361 cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
421 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
481 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
541 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
601 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
661 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
721 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
781 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
841 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
901 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
961 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
1021 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
1081 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
1141 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
1201 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
1261 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
1321 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
1381 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
1441 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
1501 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
1561 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
1621 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
1681 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
1741 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
1801 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
1861 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
1921 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
1981 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
2041 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
2101 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
2161 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
2221 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
2281 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
2341 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
2401 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
2461 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
2521 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
2581 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc gactggtgtc
2641 agaagcgggc agtctttgaa ccaccattgc aaaattataa ctcagacagt gaaagagatc
2701 tgacctaacc aattccatcg tgcttctaac ctccaagctc tccttgttca ttcctgggtg
2761 taggccgaac taagtttagg aggaacttag tttatagttt gaaacaaaga cagtaacagc
2821 cctttcccga aacaaacccc ctttttgcct ggggactaga ctgcctttgt aggactaaca
2881 aattagccaa aagattagaa attatggttt agggccaggt gcagtgactg gccccggttg
2941 tcccagcact ttgggaggct gaggctggat cacttgagcc caggagttcg agaccagcca
3001 gggccacacg gcaaaacgct gcctctacca aaaatacaaa aattaactgg ggcgtcgctt
3061 gtagtctcag ctaccaggca ggctgaggtg ggaggatcgc ttgagcttac gacgttgagg
3121 ctgcagtgag ctgtgattgc accactgcac tccagcctag gtgacagagt gagaccctgt
3181 ctcaaaaata aaaaaaaaaa aacaaaaaag aaattatggt ttaggagtca tgcagctaca
3241 ggctacaaga ttctgacctt ccccaaattg ttcctggggt aacatcacta ttgtaaaacc
3301 taagatcaat cagtgcttga gatattttgc agaccctgca ctccatggat cagctggcac
3361 ccccggattg ataaactggc tcatccgatc ttgtggcccc cacccaggaa ctgactcggc
3421 gcaaaaggac accttcgact ccctacgctt tcatctccaa ctcactggcc ccctacacac
3481 taagttatcc ttaaaaactc tgatccccga atgcttgggg agactgattt gagtcataat
3541 agaactccgt tctcccgcac agccggctct gtgtgactta ctctttctct atcctaattc
3601 ccgtcttggt gaatcggctc tgcctgggca gcaggcgggg tggacctgtt gggtgtttat
3661 gtcttgggtg tttatgtctt ggggactgag ccctcaccct gtggtctgac ctctctccag
3721 gcaggcggtg ttggaactga accgaaggac acccgccgag tctgcggcag aactgactgc
3781 tcatgtgctg gtgaaaacct ccctctccca ccacatttga tcacagaagt cttcctctgt
3841 gctgatgatt cttgtggtat cagagtagag gaaaaacagt ttcagttttc cagaaatacc
3901 tgtgaataaa atacacataa aataaaatct actaaaaata cacagagaag gggtttctcc
3961 atgttggtca ggctggtctg gaactcccaa actcaggtga tccacctgcc tcggcctccc
4021 aaagggccgg gcgcaggtgt gagccactgc gcccagccca aatatatata tttttatgac
4081 ctccatgtat aaatatattt aataccctta acttattatt attattttag acagtcttgt
4141 tttcttgccc aggctggaat gcagtggcgc gatcttggct cactgcaacc tctacctcct
4201 gggtgctaga gattttccac actgactaaa agggtctgag ggatctctag ttaccagagt
4261 cacacaacag acgggcacac actacttgaa gcactcaagg caagctttat tgaggcttaa
4321 gcagtgggtt ccctagttag ccagagagct cccaggctca gatctggtct aaccagagag
4381 acccagtaca ggcaaaaagc agctgcttat atgcagcatc tgagggctcg ccactcccca
4441 gtcccgccca ggccacgcct ccctggaaag tccccagcgg aaagtccctt gtagcaagct
4501 cgatgtcagc agttcttgaa gtactccgga tgcagctctc gggccacgtg atgaaatgct
4561 aggcggctgt caaacctcca ctctaacact tctctctcag ggtcatccat tccatgcagg
4621 ctcacagggt gtaacaagct ggtgttctct cctttattgg cctcttctac cttatctggc
4681 tcaactggta ctagcttgta gcaccatcca aaggtcagtg gatatctgac ccctggccct
4741 ggtgtgtagt tctgccaatc agggaagtag ccttgtgtgt ggtagatcca cagatcaagg
4801 atatcttgtc ttctttggga gtgaattagc ccttccagtc cccccttttc ttttaaaaag
4861 tggctaagat ctacagctgc cttgtaagtc attggtctta aaggtacctg aggtgtgact
4921 ggaaaaccca cctcttcctc ctcttgtgct tctagccagg cacaagcagc attgttagct
4981 gctgtattgc tacttgtgat tgctccatgt ttttctaggt ctcgagcaat ttggactttc
5041 cgcccttctt ggcctttatg aggatctctc tgatttttct tgcgtcgagt tttccggtaa
5101 gacctttcgg tacttcgtcc acaaacacaa ctcctccgcg caactttttc gcggttgtta
5161 cttgactggc gacgtaatcc acgatctctt tttccgtcat cgtctttccg tgctccaaaa
5221 caacaacggc ggcgggaagt tcaccggcgt catcgtcggg aagacctgcc acgcccgcgt
5281 cgaagatgtt ggggtgttgt aacaatatcg attccaattc agcgggggcc acctgatatc
5341 ctttgtattt aattaaagac ttcaagcggt caactatgaa gaagtgttcg tcttcgtccc
5401 agtaagctat gtctccagaa tgtagccatc catccttgtc aatcaaggcg ttggtcgctt
5461 ccggattgtt tacataaccg gacataatca taggtcctct gacacataat tcgcctctct
5521 gattaacgcc cagcgttttc ccggtatcca gatccacaac cttcgcttca aaaaatggaa
5581 caactttacc gaccgcgccc ggtttatcat ccccctcggg tgtaatcaga atagctgatg
5641 tagtctcagt gagcccatat ccttgtcgta tccctggaag atggaagcgt tttgcaaccg
5701 cttccccgac ttctttcgaa agaggtgcgc ccccagaagc aatttcgtgt aaattagata
5761 aatcgtattt gtcaatcaga gtgcttttgg cgaagaatga aaatagggtt ggtactagca
5821 acgcactttg aattttgtaa tcctgaaggg atcgtaaaaa cagctcttct tcaaatctat
5881 acattaagac gactcgaaat ccacatatca aatatccgag tgtagtaaac attccaaaac
5941 cgtgatggaa tggaacaaca cttaaaatcg cagtatccgg aatgatttga ttgccaaaaa
6001 taggatctct ggcatgcgag aatctgacgc aggcagttct atgcggaagg gccacaccct
6061 taggtaaccc agtagatcca gaggaattca ttatcagtgc aattgttttg tcacgatcaa
6121 aggactctgg tacaaaatcg tattcattaa aaccgggagg tagatgagat gtgacgaacg
6181 tgtacatcga ctgaaatccc tggtaatccg ttttagaatc catgataata attttctgga
6241 ttattggtaa ttttttttgc acgttcaaaa ttttttgcaa cccctttttg gaaacaaaca
6301 ctacggtagg ctgcgaaatg ttcatactgt tgagcaattc acgttcatta taaatgtcgt
6361 tcgcgggcgc aactgcaact ccgataaata acgcgcccaa caccggcata aagaattgaa
6421 gagagttttc actgcatacg acgattctgt gatttgtatt cagcccatat cgtttcatag
6481 cttctgccaa ccgaacggac atttcgaagt attccgcgta cgtgatgttc acctcgatat
6541 gtgcatctgt aaaagcaatt gttccaggaa ccagggcgta tctcttcata gccttatgca
6601 gttgctctcc agcggttcca tcctctagag gatagaatgg cgccgggcct ttctttatgt
6661 ttttggcgtc ttcagcggcc gcgccaccca tcttatagca aaatcctttc caagccctgt
6721 cttattcttc taggtatgtg gcgaatagct ctacaagctc cttgtactac ttctataacc
6781 ctatctgtcc cctcagctac tgctatggct gtggcattga gcaagctaac agcactattc
6841 tttagctcct gactccaata ttgtaggaga ttccaccaat atttgagggc ttcccacccc
6901 ctgcgtccca gaagttccac aatcctcgtt acaatcaaga gtaagtctct caagcggtgg
6961 tagctgaaga ggcacaggct ccgcagatcg tcccagataa gtgctaagga tccgttcact
7021 aatcgaatgg atctgtctct gtctctctct ccaccttctt cttctattcc ttcgggcctg
7081 tcgggtcccc tcgggattgg gaggtgggtc tgaaacgata atggtgaata tccctgccta
7141 actctattca ctatagaaag tacagcaaaa actattctta aacctaccaa gcctcctact
7201 atcattatga ataattttat ataccacagc caatttgtta tgttaaacca attccacaaa
7261 cttgcccatt tatctaattc caataattct tgttcattct tttcttgctg gttttgcgat
7321 tcttcaatta aggagtgtat taagcttgtg taattgttaa tttctctgtc ccactccatc
7381 caggtcatgt tattccaaat ctgttccaga gatttattac tccaactagc attccaaggc
7441 acagcagtgg tgcaaatgag ttttccagag caaccccaaa tccccaggag ctgttgatcc
7501 tttaggtatc tttccacagc caggattctt gcctggagct gtttgatgcc ccagactgtg
7561 agttgcaaca gatgctgttg cgcctcaata gccctcagca aattgttctg ctgctgcact
7621 atatcagaca ataattgtct ggcctgtacc gtcagcgtca ttgacgctgc gcccatagtg
7681 cttcctgctg ctcccaagaa cccaaggaac aaagctccta ttcccactgc tcttttttct
7741 ctctgcacca ctcttctctt tgccttggtg ggtgctactc ctaatggttc aatttttact
7801 actttatatt tatataattc acttctccaa ttgtccctca tatcgcctcc tccaggtctg
7861 aagatctcgg acccattgtt gttattacca ccatctcttg ttaatagcag cccagtaata
7921 tttgatgaac atctaatttg tccactgatg ggaggggcat acattgcttt tcctacttcc
7981 tgccacatgt ttataaattg ttttattctg catgggagtg tgattgtgtc acttccttca
8041 gtgttatttg acccttcagt actccaagta ctattaaacc aagtactatt aaacagttgt
8101 gttgaattac agtagaaaaa ttcccctcca caattaaaac tgtgcgttac aatttctggg
8161 tcccctcctg aggattgctt aaagattatt gttttattat ttccaaattg ttctcttaat
8221 ttgctagcta tctgttttaa agtggcattc cattttgctc tactaatgtt acaatgtgct
8281 tgtctcatat ttcctatttt tcctattgta acaaatgctc tccctggtcc cctctggata
8341 cggatacttt ttcttgtatt gttgttgggt cttgtacaat taatttctac agatgtgttc
8401 agctgtacta ttatggtttt agcattgtct gtgaaattgg cagatctaat tactacatct
8461 tcttctgcta gactgccatt taacagcagt tgagttgata ctactggcct gattccatgt
8521 gtacattgta ctgtgctgac atttgtacat ggtcctgttc cattgaacgt cttattatta
8581 cattttagaa tcgcaaaacc agccggggca caataatgta tgggaattgg ctcaaaggat
8641 acctttggac aggcctgtgt aatgactgag gtgttacaac ttatcaacct atagctggta
8701 ttatctattg gtactatatc aagtttataa aagaatgcat attctttctg caccttatct
8761 cttatgcttg tgctgatatt gaaagagcag ttttttatct ctcctttctc cattatcatt
8821 ctcccgctac tactattggt attagtatca ttcttcaaat cagtgcactt taaactaaca
8881 cagagtgggg ttaattttac acatggcttt aggctttgat cccataaact gattatatcc
8941 tcatgcatct gttctaccat gtcatttttc cacatgttaa aattttctgt cacatttacc
9001 aatactactt cttgtgggtt ggggtctgtg ggtacacagg catgtgtggc ccaaacatta
9061 tgtacctctg tatcatatat gctttagcat ctgatgcaca aaatagagtg gtggttgctt
9121 ccttccacac aggtacccca taatagactg tgacccacaa tttttctgta gcactacaga
9181 tcatcaatat cccaaggagc atggtgcccc atttccaccc ccatctccac aagtgctgat
9241 acttctcctt cactctcatt gccactgtct tctgctcttt ctattagtct atcaattaac
9301 ctgtctattt ttctttgtct taatattttc ctatattcta tgattactat ggaccacaca
9361 actattgcta ttattattgc tactactaat gctactattg ctactattat aggttgcatt
9421 acatgtacta cttactgctt tgatagagaa gcttgatgag tctgactgtt ctgatgagct
9481 cttcgtcgct gtctccgctt cttcctgcca taggagatgc ctaaggcttt tgtcatgaaa
9541 caaacttggc aatgaaagca acacttttta caatagcaat tggtacaagc agttttaggc
9601 tgacttcctg gatgcttcca gggctctagt ctaggatcta ctggctccat ttcttgctct
9661 cctctgtcga gtaacgccta ttctgctatg tcgacaccca attctgaaat ggataaacag
9721 cagttgttgc agaattctta ttatggcttc cactcctgcc caagtatccc cgtaagtttc
9781 atagatatgt tgtcctaagt tatggagcca tatcctagga aaatgtctaa cagcttcact
9841 cttaattaag ttcctctaaa agctctagtg tccattcatt gtatggctcc ctctgtggcc
9901 cttggtcttc tggggcttgt tccatctgtc ctctgtcagt ttcctaacac taggcaaagg
9961 tggctttatc tgttttggtt ttattaatgc tgctagtgcc aagtactgta gagatcctac
10021 cttgttatgt cctgcttgat attcacacct aggactaact atacgtccta atatggtatt
10081 tcttatagca gattctgaaa aacaatcaaa atagtgcaga tgaattagtt ggtctgctag
10141 gtcagggtct acttgtgtgc tatatctctt tttcctccat tctatggaga ctccctgacc
10201 caaatgccag tctctttctc ctgtatgcag accccaatat gttgttatta ctaatttagc
10261 atcccctagt gggatgtgta cttctgaact tatttttgga ttagtacttt catagtgatg
10321 tctataaaac cagtccttag ctttccttga aatatacata tggtgtttta ctaatctttt
10381 ccatgtgtta atcctcatcc tgtctacttg ccacacaatc atcacctgcc atctgttttc
10441 cataatccct gatgatcttt gcttttcttc ttggcactac ttttatgtca ctattatctt
10501 gtattactac tgccccttca cctttccaga ggagctttgc tggtcctttc caaactggat
10561 ctctgctgtc cctgtaataa acccgaaaat tttgaatttt tgtaatttgt ttttgtaatt
10621 ctttagtttg tatgtctgtt gctattatgt ctactattct ttcccctgca ctgtaccccc
10681 caatcccccc ttttctttta aaattgtgga tgaatactgc catttgtact gctgtcttaa
10741 gatgttcagc ctgatctctt acctgtccta taattttctt taattcttta ttcatagatt
10801 ctattactcc ttgactttgg ggattgtagg gaatgccaaa ttcctgcttg atccccgccc
10861 accaacaggc ggccttaact gtagtactgg tgaaattgct gccattgtct gtatgtactg
10921 tttttactgg ccatcttcct gctaatttta agaggaagta tgctgtttct tgccctgtct
10981 ctgctggaat tacttctgct tctatatatc cactggctac atgaactgct accaagataa
11041 cttttccttc taaatgtgta caatctagct gccatattcc tgggctacag tctacttgtc
11101 catgcatggc ttcccctttt agctgacatt tatcacagct ggctactatt tcttttgcta
11161 ctacaggtgg taggttaaaa tcactagcca ttgctctcca attactgtga tatttctcat
11221 gttcttcttg ggccttatct attccatcta aaaatagtac tttcctgatt ccagcactga
11281 ccaatttatc tacttgttca tttcctccaa ttcctttgtg tgctggtacc catgccaggt
11341 agactttttc cttttttatt aactgctcta ttatttgact gactaactct gattcactct
11401 tatctggttg tgcttgaatg attcccaatg catattgtga gtctgtcact atgtttactt
11461 ctaatcccga atcctgcaaa gctagatgaa ttgcttgtaa ctcagtcttc tgatttgttg
11521 tgtccgttag ggggacaact ttttgtcttc ctctgtcagt tacatatcct gcttttccta
11581 atttagtttc cctattggct gccccatcta catagaaagt ttctgctcct attatgggtt
11641 ctttctctaa ctggtaccat aacttcacta agggaggggt attgacaaac tcccactcag
11701 gaatccaggt ggcttgccaa tactctgtcc accatgcttc ccatgtttcc ttttgtatgg
11761 gtaatttaaa tttaggagtc tttccccata ttactatgct ttctgtggct attttttgta
11821 ctgcctctgt taattgtttc acatcattag tgtgggcacc cttcattctt gcatactttc
11881 ctgttttcag atttttaaat ggctcttgat aaatttgata tgtccattgg ccttgcccct
11941 gcttctgtat ttctgctatt aagtcttttg atgggtcata atacactcca tgtaccggtt
12001 cttttagaat ctccctgttt tctgccagtt ctagctctgc ttcttctgtt agtggtacta
12061 cttctgttag tgctttggtt cccctaagaa gtttacataa ttgccttact ttaatccctg
12121 cataaatctg acttgcccaa ttcaattttc ccactaattt ctgtatgtca ttgacagtcc
12181 agctgtcctt ttctggcagc actataggct gtactgtcca tttatcagga tggagttcat
12241 aacccatcca aaggaatgga ggttctttct gatgtttttt gtctggtgtg gtaaatcccc
12301 acctcaacag atgttgtctc agttcctcta tttttgttct atgctgccct atttctaagt
12361 cagatcctac atacaaatca tccatgtatt gatagatgac tatgtctgga ttttgttttc
12421 taaaaggctc taagattttt gtcatgctac actggaatat tgctggtgat cctttccatc
12481 cctgtggaag cacattgtac tgatatctaa tccctggtgt ctcattgttt atactaggta
12541 tggtaaatgc agtatacttc ctgaagtctt tatctaaggg aactgaaaaa tatgcatcgc
12601 ccacatccag tactgttact gattttttct gttttaaccc tgcaggatgt ggtattccta
12661 attgaacttc ccagaaatct tgagttctct tattaagttc tctgaaatct actaattttc
12721 tccatttagt actgtctttt ttctttatgg caaatactgg agtattgtat ggattttcag
12781 gcccaatttt tgaaattttt ccttcctttt ccatttctgt acaaatttct actaatgctt
12841 ttattttttc ttctgtcaat ggccattgtt taacttttgg gccatccatt cctggcttta
12901 attttactgg tacagtctca ataggactaa tgggaaaatt taaagtgcag ccaatctgag
12961 tcaacagatt tcttccaatt atgttgacag gtgtaggtcc tactaatact gtacctatag
13021 ctttatgtcc gcagatttct atgagtatct gatcatactg tcttactttg ataaaacctc
13081 caattccccc tatcattttt ggtttccatc ttcctggcaa attcatttct tctaatactg
13141 tatcatctgc tcctgtatct aatagagctt cctttaattg cccccctatc tttattgtga
13201 cgaggggtcg ctgccaaaga gtgatctgag ggaagctaaa ggatacagtt ccttgtctat
13261 cggctcctgc ttctgagagg gagttgttgt ctcttcccca aacctgaagc tctcttctgg
13321 tggggctgtt ggctctggtc tgctctgaag aaaattccct ggccttccct tgtgggaagg
13381 ccagatcttc cctaaaaaat tagcctgtct ctcagtacaa tctttcattt ggtgtccttc
13441 ctttccacat ttccaacagc cctttttcct aggggccctg caatttttgg ctatgtgccc
13501 ttctttgcca caattgaaac acttaacagt ctttctttgg ttcctaaaat tgcctttctg
13561 tatcattatg gtagctggat ttgttacttg gctcattgct tcagccaaaa ctcttgcttt
13621 atggccgggt ccccccactc cctgacatgc tgtcatcatt tcttctagtg tcgctcctgg
13681 tcccaatgct tttaaaatag tcttacaatc tgggttcgca ttttggacca acaaggtttc
13741 tgtcatccaa ttttttacct cttgtgaagc ttgctcggct cttagagttt tatagaatcg
13801 gtctacatag tctctaaagg gttcctttgg tccttgtctt atgtccagaa tgctggtagg
13861 gctatacatt cttactattt tatttaatcc caggattatc catcttttat agatttctcc
13921 tactgggata ggtggattat gtgtcatcca tcctatttgt tcctgaaggg tactagtagt
13981 tcctgctatg tcacttcccc ttggttctct catctggcct ggtgcaatag gccctgcatg
14041 cactggatgc aatctatccc attctgcagc ttcctcattg atggtctctt ttaacatttg
14101 catggctgct tgatgtcccc ccactgtgtt tagcatggta tttaaatctt gtggggtggc
14161 tccttctgat aatgctgaaa acatgggtat tacttctggg ctgaaagcct tctcttctac
14221 tacttttacc catgcattta aagttctagg tgatatggcc tgatgtacca tttgcccctg
14281 gaggttctgc actatagggt aattttggct gacctggctg ttgtttcctg tgtcagctgc
14341 tgcttgctgt gcctttttct tacttttgtt ttgctcttcc tctatcttat ctaaggcttc
14401 cttggtgtct tttacatcta tcctttgatg cacacaatag aggactgcta ttgtattata
14461 taatgatcta agttcttctg atcctgtctg aagggatggt tgtagctgtc ccagtatttg
14521 tctacagcct tctgatgtct ctaaaaggcc aggattaact gcgaatcgtt ctagctccct
14581 gcttgcccat actatatgtt ttagtttata ttgtttcttt ccccctggcc ttaaccgaat
14641 tttttcccat ttatctaatt ctcccccgct taataccgac gctctcgcac ccatctctct
14701 ccttctagcc tccgctagtc aaaatttttg gcgtactcac cagtcgccgc ccctcgcctc
14761 ttgccgtgcg cgcttcagca agccgagtcc tgcgtcgaga gatctcctct ggctttactt
14821 tcgctttcaa gtccctgttc gggcgccact gctagagatt ttccacactg actaaaaggg
14881 tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta ctttgagcac
14941 tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
15001 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagct gcttatatgt
15061 agcatctgag ggctcgccac tccccagtcc cgcccaggcc acacctccct ggaaagtccc
15121 cagcggaaag tcccttgtag aaagctcgat gtcagcagtc tttgtagtac tccggatgca
15181 gctctcgggc catgtgacga aatgctagga ggctgtcaaa cttccacact aatacttctc
15241 cctccgggtc ctccatccca tgctggctca tagggtgtaa caagctgttg ttctctcctt
15301 cattggcctc ttctacttgc tctggttcaa ctggtactaa cttgaagcac catccaaagg
15361 tcagtggata tctgatccct ggccctggtg tgtagttctg ccaatcaggg aagtagcctt
15421 gtgtgtggta gatccacaga tcaaggatct cttgtctttt ttgggaccaa attagccctt
15481 ccagcctttc ctggtgtaag tagaactggg gctttcccta ggttctgttc atcaggtgga
15541 tggctggaag ctgcgctggt ccctccctcc acaccaagga ctctgatgcg caggtgtgct
15601 tgcacctccg acatggcagt tcctccggcc atgtgcacgt ggagggcggc ccattgtggt
15661 agccttggga ggtgtcttgg ttatcttgtg tcactgtgac ctgtggagat ccaactgagg
15721 tagtcatcac ccctcggggg acacattcca agacccaggt ggaggcctga aatgcgatag
15781 tactgaaccc tgtatatact gttttcccct ctgggaacac acctctgatc aaatttaact
15841 tacaagttag gcacagtaga gactaaaaaa attgtgaatc aaatggaatg attgtaaggg
15901 tgcactgtga taaaccccct gtgaggctga cgactgtcag ggtgcaatgt gataaacccc
15961 ctgtgaggct gacgactgtc agggtgcact gtgataaacc ccctgtaaag ctggtctctt
16021 tctctcgcag tcttaccgct ctgtgcgcac ctagtttcag accttggctg aacgcaagta
16081 actgaaacta gggaaagcaa agccgagtgg gggcagccgc tagaaggaag gggcagcctc
16141 agggcacact gtggggcttg gcagttaggg accctgcaga gtccacatgc cagttggttg
16201 gcagcacagc agagcgcccg gaaggggcac atgcctttga caggagttag atgtggtgga
16261 gagaggcgct taaaattatt gaggacaaga tgggttattt ggtatttaat gctaacagct
16321 gtatctccat tttgatgtga aggagtgagc tagattttat ttatttttta ttattttatt
16381 ttttaccttt aggctc
//
pNL4.3-Luc HIV-1 NL4-3 ΔEnv Vpr Luciferase载体图谱质粒图谱pdf版和相关资料下载
pNL4.3-Luc HIV-1 NL4-3 ΔEnv Vpr Luciferase载体质粒应用举例
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