pET-20b(+)

价格:800元

联系方式:I47-825O-882O

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pET20b载体基本信息

出品公司: EMD Biosciences(Novagen)
别名: pET20b, pet 20b
质粒类型: 大肠杆菌蛋白表达
表达水平:
克隆方法: 多克隆位点,限制性内切酶
载体大小: 3716bp
5' 测序引物: T7
5' 测序引物序列:   5'-TAATACGACTCACTATAGGG-3'
载体标签: N-pelB信号肽, C-His
载体抗性: Ampicillin
备注:
Hosts: E.coli. Related vectors: pBR322.
产品目录号: 69337-3
稳定性: 瞬时表达 Transient
组成型: 组成型 Constitutive
病毒/非病毒: 非病毒

pET20b载体质粒图谱和多克隆位点信息

pET-20b载体图谱



pET-20b多克隆位点

pET-20b载体特征

pET20b载体简介

The pET-20b(+) vector (Cat. No. 69739-3) carries an N-terminal pelB signal sequence for potential periplasmic localization, plus optional C-terminal His•Tag® sequence. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, single-stranded sequencing should be performed using the T7 terminator primer (Cat. No. 69337-3).
pET Vectors 选择指南

pET20b载体序列

LOCUS       pET-20b(+)    3716 bp     DNA  circular   SYN
DEFINITION  pET-20b(+)
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank|
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTOAUTHORAD1|Novagen Inc.|
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="pET-20b(+)"
                     /mol_type="other DNA"
     terminator      complement(1..129)
                     /label="T7_terminator"
     misc_feature    69..87
                     /label="T7_Terminal_primer"
     misc_feature    complement(298..314)
                     /label="T7_transl_en_RBS"
     promoter        complement(351..369)
                     /label="T7_promoter"
     misc_feature    878..1069
                     /label="ROP"
     misc_feature    complement(1063..1085)
                     /label="pGEX_3_primer"
     rep_origin      complement(1484..2103)
                     /label="pBR322_origin"
     gene            complement(2258..3118)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(2258..3118)
                     /label="ORF frame 2"
     promoter        complement(3160..3188)
                     /label="AmpR_promoter"
ORIGIN
    1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
   61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
  121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
  181 cgacggagct cgaattcgga tccgaattaa ttccgatatc catggccatc gccggctggg
  241 cagcgaggag cagcagacca gcagcagcgg tcggcagcag gtatttcata tgtatatctc
  301 cttcttaaag ttaaacaaaa ttatttctag agggaaaccg ttgtggtctc cctatagtga
  361 gtcgtattaa tttcgcggga tcgagatctc gggcagcgtt gggtcctggc cacgggtgcg
  421 catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc ttactggtta
  481 gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc
  541 gacctgagca acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg
  601 gaagtcagcg ccctgcacca ttatgttccg gatctgcatc gcaggatgct gctggctacc
  661 ctgtggaaca cctacatctg tattaacgaa gcgctggcat tgaccctgag tgatttttct
  721 ctggtcccgc cgcatccata ccgccagttg tttaccctca caacgttcca gtaaccgggc
  781 atgttcatca tcagtaaccc gtatcgtgag catcctctct cgtttcatcg gtatcattac
  841 ccccatgaac agaaatcccc cttacacgga ggcatcagtg accaaacagg aaaaaaccgc
  901 ccttaacatg gcccgcttta tcagaagcca gacattaacg cttctggaga aactcaacga
  961 gctggacgcg gatgaacagg cagacatctg tgaatcgctt cacgaccacg ctgatgagct
 1021 ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
 1081 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
 1141 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag
 1201 cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
 1261 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt
 1321 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
 1381 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
 1441 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
 1501 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
 1561 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
 1621 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
 1681 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
 1741 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
 1801 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
 1861 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
 1921 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
 1981 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
 2041 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
 2101 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
 2161 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
 2221 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
 2281 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
 2341 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
 2401 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
 2461 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
 2521 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca
 2581 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
 2641 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
 2701 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
 2761 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
 2821 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
 2881 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
 2941 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
 3001 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
 3061 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
 3121 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
 3181 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
 3241 tgccacctga aattgtaaac gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
 3301 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
 3361 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
 3421 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
 3481 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
 3541 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
 3601 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
 3661 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgcca

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pET20b载体应用举例

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