| 出品公司: | EMD Biosciences(Novagen) |
|---|---|
| 别名: | pET17b, pet 17b |
| 质粒类型: | 大肠杆菌蛋白表达 |
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 3306bp |
| 5' 测序引物: | T7 |
| 5' 测序引物序列: | 5'-TAATACGACTCACTATAGGG-3' |
| 载体标签: | N-T7 |
| 载体抗性: | Ampicillin |
| 备注: |
Hosts: E.coli. Related vectors: pBR322.
|
| 产品目录号: | 69663-3 |
| 稳定性: | 瞬时表达 Transient |
| 组成型: | 组成型 Constitutive |
| 病毒/非病毒: | 非病毒 |
| 表达宿主菌: | BL21(DE3) 、BLR(DE3)、Rosetta(DE3)、OrigamiB(DE3) |
| pET表达手册： |
pET表达手册英文版 pET表达手册中文版 |
| pET载体汇总: |
pET-3a pET-11a pET-12a pET-14b pET-15b pET-16b pET-17b pET-19b pET-20b pET-21a pET-22b pET-23a pET-24a pET-25b pET-26b pET-27b pET-28a pET-29a pET-30a pET-31b pET-32a pET-33b pET-34b pET-35b pET-37b pET-39b pET-40b pET-41a pET-42a pET-43.1a pET-44a pET-45b pET-47b pET-48b pET-49b pET-50b pET-51b pET-52b pETDuet-1 pACYCDuet-1 pCDFDuet-1 pRSFDuet-1 pColaDuet-1 |
买家导航
pET-17b载体 pET17b质粒基本信息
pET-17b质粒图谱 pET17b载体图谱和pET-17b载体序列 pET17b质粒序列多克隆位点信息
pET-17b质粒 pET17b载体简介
pET-17b载体具有N端T7标签。独特的酶切位点在载体图谱中被标注出来。需要注意的是，这些载体序列是依据载体pBR322的方向来进行编号的，所以T7标签表达区域在载体图谱上是反向的。 
pET-17b质粒序列 pET17b载体序列
LOCUS pET-17b 3306 bp DNA circular SYN
DEFINITION pET-17b
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTOAUTHORAD1|Novagen Inc.|
FEATURES Location/Qualifiers
source 1..3306
/organism="pET-17b"
/mol_type="other DNA"
terminator complement(3..131)
/label="T7_terminator"
misc_feature 71..89
/label="T7_Terminal_primer"
misc_feature complement(237..269)
/label="T7_leader"
misc_feature complement(252..270)
/label="Xpress_fwd_primer"
misc_feature complement(278..294)
/label="T7_transl_en_RBS"
promoter complement(331..349)
/label="T7_promoter"
misc_feature 858..1049
/label="ROP"
misc_feature complement(1043..1065)
/label="pGEX_3_primer"
gene complement(2238..3098)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(2238..3098)
/label="ORF frame 2"
promoter complement(3140..3168)
/label="AmpR_promoter"
ORIGIN
1 ttatccggat atagttcctc ctttcagcaa aaaacccctc aagacccgtt tagaggcccc
61 aaggggttat gctagttatt gctcagcggt ggcagcagcc aactcagctt cctttcgggc
121 tttgttagca gccggatctg ctcgagcggc cgccagtgtg atggatatct gcagaattcc
181 agcacactgg cggccgttac tagtggatcc gagctcggta ccaagcttga atcccgaccc
241 atttgctgtc caccagtcat gctagccata tgtatatctc cttcttaaag ttaaacaaaa
301 ttatttctag agggaaaccg ttgtggtctc cctatagtga gtcgtattaa tttcgcggga
361 tcgagatctc gggcagcgtt gggtcctggc cacgggtgcg catgatcgtg ctcctgtcgt
421 tgaggacccg gctaggctgg cggggttgcc ttactggtta gcagaatgaa tcaccgatac
481 gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc gacctgagca acaacatgaa
541 tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca
601 ttatgttccg gatctgcatc gcaggatgct gctggctacc ctgtggaaca cctacatctg
661 tattaacgaa gcgctggcat tgaccctgag tgatttttct ctggtcccgc cgcatccata
721 ccgccagttg tttaccctca caacgttcca gtaaccgggc atgttcatca tcagtaaccc
781 gtatcgtgag catcctctct cgtttcatcg gtatcattac ccccatgaac agaaatcccc
841 cttacacgga ggcatcagtg accaaacagg aaaaaaccgc ccttaacatg gcccgcttta
901 tcagaagcca gacattaacg cttctggaga aactcaacga gctggacgcg gatgaacagg
961 cagacatctg tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc
1021 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
1081 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
1141 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa
1201 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atatgcggtg tgaaataccg
1261 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac
1321 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
1381 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
1441 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
1501 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
1561 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
1621 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
1681 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
1741 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
1801 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
1861 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
1921 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
1981 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
2041 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
2101 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
2161 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
2221 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
2281 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
2341 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
2401 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
2461 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
2521 gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg
2581 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
2641 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
2701 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
2761 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
2821 atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc
2881 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
2941 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
3001 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
3061 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
3121 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
3181 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
3241 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt
3301 caagaa
//
