pET-17b

价格:800元

联系方式:I47-825O-882O

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pET17b载体基本信息

出品公司: EMD Biosciences(Novagen)
别名: pET17b, pet 17b
质粒类型: 大肠杆菌蛋白表达
表达水平:
克隆方法: 多克隆位点,限制性内切酶
载体大小: 3306bp
5' 测序引物: T7
5' 测序引物序列:   5'-TAATACGACTCACTATAGGG-3'
载体标签: N-T7
载体抗性: Ampicillin
备注:
Hosts: E.coli. Related vectors: pBR322.
产品目录号: 69663-3
稳定性: 瞬时表达 Transient
组成型: 组成型 Constitutive
病毒/非病毒: 非病毒

pET17b载体质粒图谱和多克隆位点信息

pet17b载体图谱


pet17b载体多克隆位点


pET17b载体简介

The pET-17b vector (Cat. No. 69663-3) carries an N-terminal 11aa T7?Tag? sequence followed by a region of useful cloning sites. Included in the multiple cloning region are dual BstX I sites, which allow efficient cloning using an asymmetric linker. Unique sites (except for the two BstX I sites) are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circluar map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below.

pET17b载体序列

LOCUS       pET-17b    3306 bp     DNA   circular  SYN
DEFINITION  pET-17b
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTOAUTHORAD1|Novagen Inc.|
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="pET-17b"
                     /mol_type="other DNA"
     terminator      complement(3..131)
                     /label="T7_terminator"
     misc_feature    71..89
                     /label="T7_Terminal_primer"
     misc_feature    complement(237..269)
                     /label="T7_leader"
     misc_feature    complement(252..270)
                     /label="Xpress_fwd_primer"
     misc_feature    complement(278..294)
                     /label="T7_transl_en_RBS"
     promoter        complement(331..349)
                     /label="T7_promoter"
     misc_feature    858..1049
                     /label="ROP"
     misc_feature    complement(1043..1065)
                     /label="pGEX_3_primer"
     gene            complement(2238..3098)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(2238..3098)
                     /label="ORF frame 2"
     promoter        complement(3140..3168)
                     /label="AmpR_promoter"
ORIGIN
    1 ttatccggat atagttcctc ctttcagcaa aaaacccctc aagacccgtt tagaggcccc
   61 aaggggttat gctagttatt gctcagcggt ggcagcagcc aactcagctt cctttcgggc
  121 tttgttagca gccggatctg ctcgagcggc cgccagtgtg atggatatct gcagaattcc
  181 agcacactgg cggccgttac tagtggatcc gagctcggta ccaagcttga atcccgaccc
  241 atttgctgtc caccagtcat gctagccata tgtatatctc cttcttaaag ttaaacaaaa
  301 ttatttctag agggaaaccg ttgtggtctc cctatagtga gtcgtattaa tttcgcggga
  361 tcgagatctc gggcagcgtt gggtcctggc cacgggtgcg catgatcgtg ctcctgtcgt
  421 tgaggacccg gctaggctgg cggggttgcc ttactggtta gcagaatgaa tcaccgatac
  481 gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc gacctgagca acaacatgaa
  541 tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca
  601 ttatgttccg gatctgcatc gcaggatgct gctggctacc ctgtggaaca cctacatctg
  661 tattaacgaa gcgctggcat tgaccctgag tgatttttct ctggtcccgc cgcatccata
  721 ccgccagttg tttaccctca caacgttcca gtaaccgggc atgttcatca tcagtaaccc
  781 gtatcgtgag catcctctct cgtttcatcg gtatcattac ccccatgaac agaaatcccc
  841 cttacacgga ggcatcagtg accaaacagg aaaaaaccgc ccttaacatg gcccgcttta
  901 tcagaagcca gacattaacg cttctggaga aactcaacga gctggacgcg gatgaacagg
  961 cagacatctg tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc
 1021 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
 1081 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
 1141 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa
 1201 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atatgcggtg tgaaataccg
 1261 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac
 1321 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
 1381 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
 1441 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
 1501 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
 1561 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
 1621 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
 1681 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
 1741 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
 1801 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
 1861 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
 1921 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
 1981 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
 2041 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
 2101 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
 2161 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
 2221 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
 2281 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
 2341 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
 2401 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
 2461 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
 2521 gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg
 2581 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
 2641 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
 2701 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
 2761 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
 2821 atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc
 2881 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
 2941 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
 3001 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
 3061 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
 3121 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
 3181 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
 3241 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt
 3301 caagaa
//

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