pJG4-5

价格:1500元

联系方式:I47-825O-882O

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pJG45载体基本信息

出品公司: Gyuris J, Golemis E, Chertkov H, Brent R.
载体名称: pJG4-5
质粒类型: 酵母质粒
高拷贝/低拷贝: 高拷贝
克隆方法: --
启动子: --
载体大小: 6449 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素
筛选标记: --
克隆菌株: --
宿主细胞(系): --
备注: --
产品目录号: --
稳定性: --
组成型/诱导型: --
病毒/非病毒: --

pJG45载体质粒图谱和多克隆位点信息

pJG4-5载体图谱

pJG45载体简介

pJG45载体序列

LOCUS       pJG4-5                6449 bp ds-DNA     circular SYN 23-FEB-2014
DEFINITION  Yeast two-hybrid vector with a GAL1 promoter, for fusing the B42 
            transcriptional activator to a partner protein. Also known as 
            pB42AD.
ACCESSION   U89961.1
VERSION     .
KEYWORDS    pJG4-5
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6449)
  AUTHORS   Gyuris J, Golemis E, Chertkov H, Brent R.
  TITLE     Cdi1, a human G1 and S phase protein phosphatase that associates 
            with Cdk2.
  JOURNAL   Cell 1993;75:791-803.
  PUBMED    8242750
REFERENCE   2  (bases 1 to 6449)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, February 14, 2020 from SnapGene Viewer 3.1.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6449
                     /organism="synthetic DNA construct"
                     /lab_host="Saccharomyces cerevisiae"
                     /mol_type="other DNA"
     promoter        81..522
                     /gene="S. cerevisiae GAL1"
                     /note="GAL1 promoter"
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    81..198
                     /bound_moiety="Gal4"
                     /note="UAS"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     CDS             534..536
                     /codon_start=1
                     /product="start codon"
                     /note="ATG"
                     /translation="M"
     CDS             546..566
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     CDS             573..809
                     /codon_start=1
                     /product="yeast transcriptional activator cretaed from E. 
                     coli genomic DNA fragments (Ma and Ptashne, 1987)"
                     /note="B42 transcriptional activator"
                     /translation="INKDIEECNAIIEQFIDYLRTGQEMPMEMADQAINVVPGMTPKTI
                     LHAGPPIQPDWLKSNGFHEIEADVNDTSLLLSGD"
     CDS             816..842
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /note="HA"
                     /translation="YPYDVPDYA"
     misc_feature    849..866
                     /note="MCS"
                     /note="multiple cloning site"
     terminator      1004..1191
                     /gene="S. cerevisiae ADH1"
                     /note="ADH1 terminator"
                     /note="transcription terminator for alcohol dehydrogenase 
                     1"
     rep_origin      1713..3055
                     /note="2u ori"
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(3416..4090)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /note="TRP1"
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(4091..4192)
                     /gene="S. cerevisiae TRP1"
                     /note="TRP1 promoter"
     promoter        4295..4399
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             4403..5263
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="SIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIE
                     LDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYSP
                     VTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWE
                     PELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSAL
                     PAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASL
                     IKHW*L"
     rep_origin      5431..6019
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ccccattatc ttagcctaaa aaaaccttct ctttggaact ttcagtaata cgcttaactg
       61 ctcattgcta tattgaagta cggattagaa gccgccgagc gggtgacagc cctccgaagg
      121 aagactctcc tccgtgcgtc ctcgtcttca ccggtcgcgt tcctgaaacg cagatgtgcc
      181 tcgcgccgca ctgctccgaa caataaagat tctacaatac tagcttttat ggttatgaag
      241 aggaaaaatt ggcagtaacc tggccccaca aaccttcaaa tgaacgaatc aaattaacaa
      301 ccataggatg ataatgcgat tagtttttta gccttatttc tggggtaatt aatcagcgaa
      361 gcgatgattt ttgatctatt aacagatata taaatgcaaa aactgcataa ccactttaac
      421 taatactttc aacattttcg gtttgtatta cttcttattc aaatgtaata aaagtatcaa
      481 caaaaaattg ttaatatacc tctatacttt aacgtcaagg aggaattaag cttatgggtg
      541 ctcctccaaa aaagaagaga aaggtagctg gtatcaataa agatatcgag gagtgcaatg
      601 ccatcattga gcagtttatc gactacctgc gcaccggaca ggagatgccg atggaaatgg
      661 cggatcaggc gattaacgtg gtgccgggca tgacgccgaa aaccattctt cacgccgggc
      721 cgccgatcca gcctgactgg ctgaaatcga atggttttca tgaaattgaa gcggatgtta
      781 acgataccag cctcttgctg agtggagatg cctcctaccc ttatgatgtg ccagattatg
      841 cctctcccga attcggccga ctcgagaagc tttggacttc ttcgccagag gtttggtcaa
      901 gtctccaatc aaggttgtcg gcttgtctac cttgccagaa atttacgaaa agatggaaaa
      961 gggtcaaatc gttggtagat acgttgttga cacttctaaa taagcgaatt tcttatgatt
     1021 tatgattttt attattaaat aagttataaa aaaaataagt gtatacaaat tttaaagtga
     1081 ctcttaggtt ttaaaacgaa aattcttgtt cttgagtaac tctttcctgt aggtcaggtt
     1141 gctttctcag gtatagcatg aggtcgctct tattgaccac acctctaccg gcatgccgag
     1201 caaatgcctg caaatcgctc cccatttcac ccaattgtag atatgctaac tccagcaatg
     1261 agttgatgaa tctcggtgtg tattttatgt cctcagagga caacacctgt tgtaatcgtt
     1321 cttccacacg gatcctctag agtcgactag cggccgcttc gacctgcagc aattctgaac
     1381 cagtcctaaa acgagtaaat aggaccggca attcttcaag caataaacag gaataccaat
     1441 tattaaaaga taacttagtc agatcgtaca ataaagcttt gaagaaaaat gcgccttatt
     1501 caatctttgc tataaaaaat ggcccaaaat ctcacattgg aagacatttg atgacctcat
     1561 ttctttcaat gaagggccta acggagttga ctaatgttgt gggaaattgg agcgataagc
     1621 gtgcttctgc cgtggccagg acaacgtata ctcatcagat aacagcaata cctgatcact
     1681 acttcgcact agtttctcgg tactatgcat atgatccaat atcaaaggaa atgatagcat
     1741 tgaaggatga gactaatcca attgaggagt ggcagcatat agaacagcta aagggtagtg
     1801 ctgaaggaag catacgatac cccgcatgga atgggataat atcacaggag gtactagact
     1861 acctttcatc ctacataaat agacgcatat aagtacgcat ttaagcataa acacgcacta
     1921 tgccgttctt ctcatgtata tatatataca ggcaacacgc agatataggt gcgacgtgaa
     1981 cagtgagctg tatgtgcgca gctcgcgttg cattttcgga agcgctcgtt ttcggaaacg
     2041 ctttgaagtt cctattccga agttcctatt ctctagaaag tataggaact tcagagcgct
     2101 tttgaaaacc aaaagcgctc tgaagacgca ctttcaaaaa accaaaaacg caccggactg
     2161 taacgagcta ctaaaatatt gcgaataccg cttccacaaa cattgctcaa aagtatctct
     2221 ttgctatata tctctgtgct atatccctat ataacctacc catccacctt tcgctccttg
     2281 aacttgcatc taaactcgac ctctacattt tttatgttta tctctagtat tactctttag
     2341 acaaaaaaat tgtagtaaga actattcata gagtgaatcg aaaacaatac gaaaatgtaa
     2401 acatttccta tacgtagtat atagagacaa aatagaagaa accgttcata attttctgac
     2461 caatgaagaa tcatcaacgc tatcactttc tgttcacaaa gtatgcgcaa tccacatcgg
     2521 tatagaatat aatcggggat gcctttatct tgaaaaaatg cacccgcagc ttcgctagta
     2581 atcagtaaac gcgggaagtg gagtcaggct ttttttatgg aagagaaaat agacaccaaa
     2641 gtagccttct tctaacctta acggacctac agtgcaaaaa gttatcaaga gactgcatta
     2701 tagagcgcac aaaggagaaa aaaagtaatc taagatgctt tgttagaaaa atagcgctct
     2761 cgggatgcat ttttgtagaa caaaaaagaa gtatagattc tttgttggta aaatagcgct
     2821 ctcgcgttgc atttctgttc tgtaaaaatg cagctcagat tctttgtttg aaaaattagc
     2881 gctctcgcgt tgcatttttg ttttacaaaa atgaagcaca gattcttcgt tggtaaaata
     2941 gcgctttcgc gttgcatttc tgttctgtaa aaatgcagct cagattcttt gtttgaaaaa
     3001 ttagcgctct cgcgttgcat ttttgttcta caaaatgaag cacagatgct tcgttaacaa
     3061 agatatgcta ttgaagtgca agatggaaac gcagaaaatg aaccggggat gcgacgtgca
     3121 agattaccta tgcaatagat gcaatagttt ctccaggaac cgaaatacat acattgtctt
     3181 ccgtaaagcg ctagactata tattattata caggttcaaa tatactatct gtttcaggga
     3241 aaactcccag gttcggatgt tcaaaattca atgatgggta acaagtacga tcgtaaatct
     3301 gtaaaacagt ttgtcggata ttaggctgta tctcctcaaa gcgtattcga atatcattga
     3361 gaagctgcag gcaagtgcac aaacaatact taaataaata ctactcagta ataacctatt
     3421 tcttagcatt tttgacgaaa tttgctattt tgttagagtc ttttacacca tttgtctcca
     3481 cacctccgct tacatcaaca ccaataacgc catttaatct aagcgcatca ccaacatttt
     3541 ctggcgtcag tccaccagct aacataaaat gtaagctttc ggggctctct tgccttccaa
     3601 cccagtcaga aatcgagttc caatccaaaa gttcacctgt cccacctgct tctgaatcaa
     3661 acaagggaat aaacgaatga ggtttctgtg aagctgcact gagtagtatg ttgcagtctt
     3721 ttggaaatac gagtctttta ataactggca aaccgaggaa ctcttggtat tcttgccacg
     3781 actcatctcc atgcagttgg acgatatcaa tgccgtaatc attgaccaga gccaaaacat
     3841 cctccttagg ttgattacga aacacgccaa ccaagtattt cggagtgcct gaactatttt
     3901 tatatgcttt tacaagactt gaaattttcc ttgcaataac cgggtcaatt gttctctttc
     3961 tattgggcac acatataata cccagcaagt cagcatcgga atctagagca cattctgcgg
     4021 cctctgtgct ctgcaagccg caaactttca ccaatggacc agaactacct gtgaaattaa
     4081 taacagacat actccaagct gcctttgtgt gcttaatcac gtatactcac gtgctcaata
     4141 gtcaccaatg ccctccctct tggccctctc cttttctttt ttcgaccgaa tttcttgaag
     4201 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     4261 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     4321 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     4381 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     4441 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     4501 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     4561 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     4621 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca
     4681 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     4741 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     4801 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     4861 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     4921 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     4981 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     5041 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     5101 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     5161 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     5221 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     5281 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     5341 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     5401 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     5461 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     5521 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     5581 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     5641 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     5701 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     5761 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     5821 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     5881 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     5941 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     6001 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     6061 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     6121 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     6181 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     6241 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     6301 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     6361 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     6421 catgattacg aattaattcg agctcggta
//

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