| 质粒名称: | pTetR-LasR-pLuxR-GFP |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 171159 |
| 存储实验室: | Matthew Chang |
|
载体骨架基本信息 |
|
| 载体名称: | pSB1A2 |
| 载体出品公司: | -- |
| 空载体大小: | 2079 bp |
| 完整质粒大小: | 3966 bp |
| 载体修饰: | -- |
| 载体类型: | 细菌表达，合成生物学 |
| 筛选标记: | -- |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | lasR,transcriptional regulator LasR |
| 物种来源: | Pseudomonas aeruginosa |
| 插入大小: | 723 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | pTet |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | XbaI（克隆期间销毁） |
| 3’克隆位点: | SpeI （克隆期间销毁） |
| 5’测序引物 | Nil |
| 3’测序引物 | Nil |
|
文献引用方法 |
|
| 材料和方法部分: | pTetR-LasR-pLuxR-GFP was a gift from Matthew Chang (Addgene plasmid # 171159 ; http://n2t.net/addgene:171159 ; RRID:Addgene_171159) |
| 参考文献部分: | Engineering microbes to sense and eradicate Pseudomonas aeruginosa, a human pathogen. Saeidi N, Wong CK, Lo TM, Nguyen HX, Ling H, Leong SS, Poh CL, Chang MW. Mol Syst Biol. 2011 Aug 16;7:521. doi: 10.1038/msb.2011.55. 10.1038/msb.2011.55 PubMed 21847113 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pTetR-LasR-pLuxR-GFP 3977 bp ds-DNA circular SYN 15-JUL-2021
DEFINITION Sensing Device with Reporter protein.
ACCESSION .
VERSION .
KEYWORDS pTetR-LasR-pLuxR-GFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3977)
AUTHORS Saeidi N, Wong CK, Lo TM, Nguyen HX, Ling H, Leong SS, Poh CL, Chang
MW
TITLE Engineering microbes to sense and eradicate Pseudomonas aeruginosa,
a human pathogen.
JOURNAL Mol Syst Biol. 2011 Aug 16;7:521. doi: 10.1038/msb.2011.55.
PUBMED 21847113
REFERENCE 2 (bases 1 to 3977)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 15, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..3977
/organism="synthetic DNA construct"
/mol_type="other DNA"
terminator 83..154
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 170..197
/label=T7Te terminator
/note="phage T7 early transcription terminator"
misc_feature 204..224
/label=BioBrick suffix
/note="universal suffix for all parts"
primer_bind complement(230..247)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(412..1000)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(492..511)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(1171..2031)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 1794..1813
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(2032..2136)
/gene="bla"
/label=AmpR promoter
primer_bind 2204..2222
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
terminator 2226..2269
/label=bacterial terminator
/note="putative bacterial transcription terminator"
misc_feature 2272..2293
/label=BioBrick prefix
/note="BioBrick prefix for parts that do not start with
""ATG"""
protein_bind 2294..2312
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 2319..2337
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
RBS 2356..2367
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
terminator 3113..3184
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 3200..3227
/label=T7Te terminator
/note="phage T7 early transcription terminator"
CDS 3324..63
/codon_start=1
/product="Aequoria victoria green fluorescent protein"
/label=GFP
/translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITHGMDELYK"
primer_bind complement(3354..3382)
/label=GFP-R
/note="GFP, reverse primer. Does NOT anneal to EGFP"
primer_bind 3977..20
/label=GFP-F
/note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
1 gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa
61 taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
121 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
181 gggtgggcct ttctgcgttt atatactagt agcggccgct gcaggcttcc tcgctcactg
241 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
301 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
361 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccacag
421 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
481 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
541 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
601 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
661 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
721 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
781 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
841 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
901 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
961 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
1021 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
1081 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
1141 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
1201 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
1261 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
1321 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
1381 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
1441 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
1501 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
1561 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
1621 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
1681 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
1741 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
1801 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
1861 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
1921 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
1981 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
2041 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
2101 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
2161 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
2221 gcagaatttc agataaaaaa aatccttagc tttcgctaag gatgatttct ggaattcgcg
2281 gccgcttcta gagtccctat cagtgataga gattgacatc cctatcagtg atagagatac
2341 tgagcactac tagagaaaga ggagaaatac tagatggcct tggttgacgg ttttcttgag
2401 ctggaacgct caagtggaaa attggagtgg agcgccatcc tccagaagat ggcgagcgac
2461 cttggattct cgaagatcct gttcggcctg ttgcctaagg acagccagga ctacgagaac
2521 gccttcatcg tcggcaacta cccggccgcc tggcgcgagc attacgaccg ggctggctac
2581 gcgcgggtcg acccgacggt cagtcactgt acccagagcg tactgccgat tttctgggaa
2641 ccgtccatct accagacgcg aaagcagcac gagttcttcg aggaagcctc ggccgccggc
2701 ctggtgtatg ggctgaccat gccgctgcat ggtgctcgcg gcgaactcgg cgcgctgagc
2761 ctcagcgtgg aagcggaaaa ccgggccgag gccaaccgtt tcatagagtc ggtcctgccg
2821 accctgtgga tgctcaagga ctacgcactg caaagcggtg ccggactggc cttcgaacat
2881 ccggtcagca aaccggtggt tctgaccagc cgggagaagg aagtgttgca gtggtgcgcc
2941 atcggcaaga ccagttggga gatatcggtt atctgcaact gctcggaagc caatgtgaac
3001 ttccatatgg gaaatattcg gcggaagttc ggtgtgacct cccgccgcgt agcggccatt
3061 atggccgtta atttgggtct tattactctc taataatact agagccaggc atcaaataaa
3121 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
3181 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga
3241 gacctgtagg atcgtacagg tttacgcaag aaaatggttt gttatagtcg aataaatact
3301 agagtcacac aggaaagtac tagatgcgta aaggagaaga acttttcact ggagttgtcc
3361 caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg
3421 gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac
3481 tacctgttcc atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga
3541 gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg
3601 tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca
3661 agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag
3721 atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca
3781 tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag
3841 atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg
3901 tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg
3961 aaaagagaga ccacatg
//
