pET-16b

价格:800元

联系方式:I47-825O-882O

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pet16b载体基本信息

出品公司: EMD Biosciences(Novagen)
别名: pET16b, pet 16b
质粒类型: 大肠杆菌蛋白表达
表达水平:
克隆方法: 多克隆位点,限制性内切酶
载体大小: 5711bp
5' 测序引物: T7
5' 测序引物序列:   5'-TAATACGACTCACTATAGGG-3'
载体标签: N-His, N-Factor Xa蛋白酶切位点
载体抗性: Ampicillin
备注:
Hosts: E.coli. Related vectors: pBR322.
产品目录号: 69662-3
稳定性: 瞬时表达 Transient
组成型: 组成型 Constitutive
病毒/非病毒: 非病毒

pet16b载体质粒图谱和多克隆位点信息

pET-16b载体图谱
pET-16b多克隆位点

pET-16b载体特征

Feature Name Start End  
T7_terminator 316 188
T7_Terminal_primer 256 274
T7_transl_en_RBS 419 403
lacO 464 437
T7_promoter 482 464
pBRrevBam_primer 585 566
lacI 860 1951
tet (576 - 1190) 2010 2624
ROP 3263 3454
pGEX_3_primer 3470 3448
Ampicillin 5503 4643
AmpR_promoter 5573 5545
ORF Start End  
ORF frame 1 619 1119
ORF frame 2 992 1951
ORF frame 1 2429 1734
ORF frame 3 2007 2624
ORF frame 2 5503 4643
Enzyme Name Cut
ClaI 24
HindIII 29
BamHI 319
XhoI 324
NdeI 331
NcoI 392
XbaI 431
BglII 497
BclI 1233
ApaI 1430
HpaI 1725
EagI 2287
NruI 2322
MscI 2794
PstI 4961
AatII 5638
EcoRI 5709

pet16b载体简介

The pET-16b vector (Cat. No. 69662-3) carries an N-terminal His•Tag® sequence followed by a Factor Xa site and three cloning sites. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below.
pET Vectors 选择指南

pet16b载体序列

LOCUS       pET-16b    5711 bp     DNA  circular   SYN
DEFINITION  pET-16b
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTOAUTHORAD1|Novagen Inc.|
FEATURES             Location/Qualifiers
     source          1..5711
                     /organism="pET-16b"
                     /mol_type="other DNA"
     terminator      complement(188..316)
                     /label="T7_terminator"
     misc_feature    256..274
                     /label="T7_Terminal_primer"
     misc_feature    complement(403..419)
                     /label="T7_transl_en_RBS"
     misc_feature    complement(437..464)
                     /label="lacO"
     promoter        complement(464..482)
                     /label="T7_promoter"
     misc_feature    complement(566..585)
                     /label="pBRrevBam_primer"
     CDS             619..1119
                     /label="ORF frame 1"
                     /translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF
                     LMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECETSNVIRCRRVCRCLLSD
                     RFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTVVADWRCHLQSGPARAV
                     ANCRGD*"
     misc_feature    860..1951
                     /label="lacI"
                     /translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF
                     LMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECETSNVIRCRRVCRCLLSD
                     RFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTVVADWRCHLQSGPARAV
                     ANCRGD*"
     CDS             992..1951
                     /label="ORF frame 2"
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                     SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
     CDS             complement(1734..2429)
                     /label="ORF frame 1"
                     /translation="MVVIYLPGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQ
                     RVGRHAGDNGLLLAETFGGGTSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPER
                     CRHLSYELHDKEDSHKCGDDSHAPRPPEGADWVEGSQGHRSRSRCLMSELTYINCVAL
                     TARFPVGKPVVPAALMNRPTRGERRFAYWAPGWFFFSPVRRATADCPSPPGPERVAAS
                     GPRWFAPAGENPV*"
     CDS             2007..2624
                     /label="ORF frame 3"
                     /translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
                     FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
                     LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG
                     PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
     gene            2010..2624
                     /label="tet (576 - 1190)"
                     /gene="tet (576 - 1190)"
                     /translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
                     FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
                     LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG
                     PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
     misc_feature    3263..3454
                     /label="ROP"
                     /translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
                     FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
                     LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG
                     PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
     misc_feature    complement(3448..3470)
                     /label="pGEX_3_primer"
                     /translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
                     FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
                     LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG
                     PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
     gene            complement(4643..5503)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
                     FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
                     LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG
                     PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
     CDS             complement(4643..5503)
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(5545..5573)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
   61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
  121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
  181 gcgggatatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag
  241 gccccaaggg gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt
  301 cgggctttgt tagcagccgg atcctcgagc atatgacgac cttcgatatg gccgctgctg
  361 tgatgatgat gatgatgatg atgatgatgg cccatggtat atctccttct taaagttaaa
  421 caaaattatt tctagagggg aattgttatc cgctcacaat tcccctatag tgagtcgtat
  481 taatttcgcg ggatcgagat ctcgatcctc tacgccggac gcatcgtggc cggcatcacc
  541 ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg
  601 gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg
  661 gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc
  721 aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt
  781 cgagatcccg gacaccatcg aatggcgcaa aacctttcgc ggtatggcat gatagcgccc
  841 ggaagagagt caattcaggg tggtgaatgt gaaaccagta acgttatacg atgtcgcaga
  901 gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc
  961 tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg
 1021 cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct
 1081 ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg
 1141 tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt
 1201 gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca
 1261 ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc
 1321 tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt
 1381 ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc
 1441 tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca
 1501 gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca
 1561 aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct
 1621 gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt
 1681 gggatacgac gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca
 1741 ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca
 1801 ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc
 1861 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
 1921 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgta agttagctca
 1981 ctcattaggc accgggatct cgaccgatgc ccttgagagc cttcaaccca gtcagctcct
 2041 tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc
 2101 aactcgtagg acaggtgccg gcagcgctct gggtcatttt cggcgaggac cgctttcgct
 2161 ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc
 2221 aagccttcgt cactggtccc gccaccaaac gtttcggcga gaagcaggcc attatcgccg
 2281 gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg
 2341 ccttccccat tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca
 2401 tgctgtccag gcaggtagat gacgaccatc agggacagct tcaaggatcg ctcgcggctc
 2461 ttaccagcct aacttcgatc actggaccgc tgatcgtcac ggcgatttat gccgcctcgg
 2521 cgagcacatg gaacgggttg gcatggattg taggcgccgc cctatacctt gtctgcctcc
 2581 ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac ctgaatggaa gccggcggca
 2641 cctcgctaac ggattcacca ctccaagaat tggagccaat caattcttgc ggagaactgt
 2701 gaatgcgcaa accaaccctt ggcagaacat atccatcgcg tccgccatct ccagcagccg
 2761 cacgcggcgc atctcgggca gcgttgggtc ctggccacgg gtgcgcatga tcgtgctcct
 2821 gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga atgaatcacc
 2881 gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac
 2941 atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt cagcgccctg
 3001 caccattatg ttccggatct gcatcgcagg atgctgctgg ctaccctgtg gaacacctac
 3061 atctgtatta acgaagcgct ggcattgacc ctgagtgatt tttctctggt cccgccgcat
 3121 ccataccgcc agttgtttac cctcacaacg ttccagtaac cgggcatgtt catcatcagt
 3181 aacccgtatc gtgagcatcc tctctcgttt catcggtatc attaccccca tgaacagaaa
 3241 tcccccttac acggaggcat cagtgaccaa acaggaaaaa accgccctta acatggcccg
 3301 ctttatcaga agccagacat taacgcttct ggagaaactc aacgagctgg acgcggatga
 3361 acaggcagac atctgtgaat cgcttcacga ccacgctgat gagctttacc gcagctgcct
 3421 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
 3481 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
 3541 tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg
 3601 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatatg cggtgtgaaa
 3661 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca
 3721 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
 3781 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
 3841 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
 3901 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
 3961 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
 4021 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
 4081 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
 4141 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
 4201 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
 4261 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
 4321 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
 4381 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
 4441 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
 4501 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
 4561 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
 4621 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
 4681 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
 4741 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
 4801 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
 4861 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
 4921 cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct
 4981 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
 5041 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
 5101 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
 5161 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
 5221 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac
 5281 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
 5341 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
 5401 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
 5461 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
 5521 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
 5581 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
 5641 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
 5701 gtcttcaaga a
//

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