pET-42b(+)

价格:800元

联系方式:I47-825O-882O

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pet42b载体基本信息

出品公司: EMD Biosciences(Novagen)
别名: pET42b, pet 42b, pET-42b(+)
质粒类型: 大肠杆菌蛋白表达
表达水平:
克隆方法: 多克隆位点,限制性内切酶
载体大小: 5929bp
5' 测序引物: T7
5' 测序引物序列:   5'-TAATACGACTCACTATAGGG-3'
载体标签: N-GST, N-His, C-His
载体抗性: Kanamycin
备注:
含有GST融合蛋白;N端Thrombin蛋白酶切位点; 含有N端Factor Xa蛋白酶切位点 ; pET 42 a,b,c 的差异仅在多克隆位点区域。
产品目录号: 70562-3
稳定性: 瞬时表达 Transient
组成型: 组成型 Constitutive
病毒/非病毒: 非病毒

pet42b载体质粒图谱和多克隆位点信息

pet42b载体图谱
pet42b多克隆位点

Feature Name Start End  
T7_terminator 120 1
T7_Terminal_primer 69 87
S15 350 306
6xHIS 410 393
GST (variant) 1091 441
T7_transl_en_RBS 1116 1100
lacO 1161 1134
T7_promoter 1179 1161
tet (300 - 563) 1215 1478
pBRrevBam_primer 1286 1267
lacI 1561 2652
ROP 3224 3415
pGEX_3_primer 3431 3409
pBR322_origin 4449 3830
KanR2 4555 5370
ORF Start End  
ORF frame 3 1091 147
ORF frame 3 1092 1820
ORF frame 1 1693 2652
ORF frame 1 4555 5370
Enzyme Name Cut
XhoI 174
NotI 182
EagI 182
HindIII 189
SalI 195
SacI 206
PstI 214
AscI 216
StuI 226
EcoRI 235
BamHI 241
EcoRV 251
NcoI 254
AgeI 288
KpnI 295
BglII 298
SacII 389
SpeI 420
MscI 883
NdeI 1090
XbaI 1128
ApaI 2131
HpaI 2426
NruI 4643
SmaI 4860
XmaI 4858

pet42b载体简介

The pET-42 series is designed for cloning and high-level expression of peptide sequencesfused with the 220 aa GST•Tag™ protein. Unique sites are shown on the circle map. Note that thesequence is numbered by the pBR322 convention, so the T7 expression region is reversed on thecircle map. The cloning/expression region of the coding strand transcribed by T7 RNA polymeraseis shown below. The f1 origin is oriented so that infection with helper phage will produce virionscontaining single stranded DNA that corresponds to the coding strand. Therefore, single strandedsequencing should be performed using the T7 terminator primer (cat. no. 69337-3). Vector encodedsequence can be completely removed when cloning into the PshAI site (as shown below) and thencleaving the GST fusion protein with Factor Xa.

pet42b载体序列

LOCUS       pET-42b(+)    5929 bp     DNA  circular   SYN
DEFINITION  pET-42b(+), pet42b
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI  
                          http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|Biofeng.com|
COMMENT     VNTOAUTHORAD1|Novagen Inc.|
FEATURES             Location/Qualifiers
     source          1..5929
                     /organism="pET-42 b(+)"
                     /mol_type="other DNA"
     terminator      complement(1..120)
                     /label="T7_terminator"
     misc_feature    69..87
                     /label="T7_Terminal_primer"
     CDS             complement(147..1091)
                     /label="ORF frame 3"
     misc_feature    complement(306..350)
                     /label="S15"
     misc_feature    complement(393..410)
                     /label="6xHIS"
     gene            complement(441..1091)
                     /label="GST (variant)"
                     /gene="GST (variant)"
     CDS             1092..1820
                     /label="ORF frame 3"
     misc_feature    complement(1100..1116)
                     /label="T7_transl_en_RBS"
     misc_feature    complement(1134..1161)
                     /label="lacO"
     promoter        complement(1161..1179)
                     /label="T7_promoter"
     gene            1215..1478
                     /label="tet (300 - 563)"
                     /gene="tet (300 - 563)"
     misc_feature    complement(1267..1286)
                     /label="pBRrevBam_primer"
     misc_feature    1561..2652
                     /label="lacI"
     CDS             1693..2652
                     /label="ORF frame 1"
     misc_feature    3224..3415
                     /label="ROP"
     misc_feature    complement(3409..3431)
                     /label="pGEX_3_primer"
     rep_origin      complement(3830..4449)
                     /label="pBR322_origin"
     gene            4555..5370
                     /label="KanR2"
                     /gene="KanR2"
     CDS             4555..5370
                     /label="ORF frame 1"
ORIGIN
    1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
   61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
  121 tgtttagcag cctaggtatt aatcaattag tggtggtggt ggtggtggtg gtgctcgagt
  181 gcggccgcaa gcttgtcgac ggagctcgcc tgcaggcgcg ccaaggcctg tacagaattc
  241 ggatccccga tatccatgga cccgcgtccc tcaataccgg agccaccacc ggtacccaga
  301 tctgggctgt ccatgtgctg gcgttcgaat ttagcagcag cggtttcttt cataccaatt
  361 gcagtactac cgcgtggcac cagacccgcg gagtgatggt gatggtgatg accagaacca
  421 ctagttgaac catccgattt tggaggatgg tcgccaccac caaacgtggc ttgccagccc
  481 tgcaaaggcc atgctatata cttgctggat ttcaagtact tatcaatttg tgggatagct
  541 tcaatacgtt ttttaaaaca aactaatttt gggaacgcat ccaggcacat tgggtccatg
  601 tataaaacaa catcaagagc gtcatacaac atgaagtcag gatgggttac atgatcacca
  661 tttaaatatg ttttatgaca taaacgatct tcgaacattt tcagcatttc aggtagcttg
  721 ctaagaaaat caactttgag agtttcaaag tctttactat atgcaattct cgaaacaccg
  781 tatctaatat ccaaaaccgc tccttcaagc attgaaatct ctgcacgctc ttttggacaa
  841 ccacccaaca tgttgtgctt gtcagctata taacgtatga tggccataga ctgtgttaat
  901 ttaacatcac catcaatata ataaggaaga ttgggaaact ccaaacccaa ttcaaacttt
  961 ttgtttcgcc atttatcacc ttcatcgcgc tcatacaaat gctcttcata tttttcttca
 1021 agatattcca aaagaagtcg agtgggttgc acaaggccct taattttcca ataacctagt
 1081 ataggggaca tatgtatatc tccttcttaa agttaaacaa aattatttct agaggggaat
 1141 tgttatccgc tcacaattcc cctatagtga gtcgtattaa tttcgcggga tcgagatcga
 1201 tctcgatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg
 1261 ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca
 1321 tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg
 1381 ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac
 1441 tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgagatccc ggacaccatc
 1501 gaatggcgca aaacctttcg cggtatggca tgatagcgcc cggaagagag tcaattcagg
 1561 gtggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat
 1621 cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa
 1681 gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg
 1741 ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg
 1801 caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg
 1861 atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa
 1921 cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa
 1981 gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac
 2041 agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg
 2101 ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt
 2161 ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa
 2221 ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc
 2281 gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt
 2341 accgagtccg ggctgcgcgt tggtgcggac atctcggtag tgggatacga cgataccgaa
 2401 gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg
 2461 caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag
 2521 ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc
 2581 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
 2641 agcgggcagt gagcgcaacg caattaatgt aagttagctc actcattagg caccgggatc
 2701 tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat
 2761 gactagcatg atcgtgctcc tgtcgttgag gacccggcta ggctggcggg gttgccttac
 2821 tggttagcag aatgaatcac cgatacgcga gcgaacgtga agcgactgct gctgcaaaac
 2881 gtctgcgacc tgagcaacaa catgaatggt cttcggtttc cgtgtttcgt aaagtctgga
 2941 aacgcggaag tcagcgccct gcaccattat gttccggatc tgcatcgcag gatgctgctg
 3001 gctaccctgt ggaacaccta catctgtatt aacgaagcgc tggcattgac cctgagtgat
 3061 ttttctctgg tcccgccgca tccataccgc cagttgttta ccctcacaac gttccagtaa
 3121 ccgggcatgt tcatcatcag taacccgtat cgtgagcatc ctctctcgtt tcatcggtat
 3181 cattaccccc atgaacagaa atccccctta cacggaggca tcagtgacca aacaggaaaa
 3241 aaccgccctt aacatggccc gctttatcag aagccagaca ttaacgcttc tggagaaact
 3301 caacgagctg gacgcggatg aacaggcaga catctgtgaa tcgcttcacg accacgctga
 3361 tgagctttac cgcagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
 3421 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
 3481 tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag
 3541 cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg
 3601 caccatatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
 3661 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
 3721 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
 3781 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
 3841 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
 3901 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
 3961 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
 4021 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
 4081 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
 4141 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
 4201 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
 4261 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
 4321 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
 4381 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
 4441 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
 4501 tggtcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc
 4561 catattcaac gggaaacgtc ttgctctagg ccgcgattaa attccaacat ggatgctgat
 4621 ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcga
 4681 ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc
 4741 aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg
 4801 accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc
 4861 gggaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat
 4921 gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac
 4981 agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat
 5041 gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg
 5101 cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat
 5161 aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc
 5221 gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca
 5281 ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag
 5341 tttcatttga tgctcgatga gtttttctaa gaattaattc atgagcggat acatatttga
 5401 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
 5461 tgaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
 5521 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
 5581 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
 5641 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
 5701 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
 5761 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
 5821 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
 5881 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgcca
//

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pet42b载体应用举例

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