| 出品公司: | Agilent |
|---|---|
| 载体名称: | pDM4 |
| 质粒类型: | ORF |
| 高拷贝/低拷贝: | 低拷贝 |
| 启动子: | R6K |
| 载体大小: | 7.1kb |
| 5’测序引物及序列: | P0103-F：gtgacaatcacgaaacgcgg |
| 3’测序引物及序列: | P0103-R：ggtatttattcggcgcaaagtgc |
| 感受态: | S17-1λpir |
| 载体抗性: | Chl |
| 筛选标记: | SacB |
| 克隆菌株: | DH5 alpha; 等 |
| 宿主细胞（系）: | 大肠杆菌 |
| 备注: | 基因编辑 |
| 产品目录号: | #0103 |
| 物种: | 空载体 |
| 温度: | 37 |
| 病毒/非病毒: | 非病毒 |
买家导航
pDM4载体质粒基本信息
pDM4质粒图谱载体图谱和pDM4载体序列质粒序列多克隆位点信息
pDM4质粒载体简介
pDM4质粒序列载体序列
LOCUS pDM4 7104
bp DNA circular SYN 24-JUL-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7104)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 24, 2024 from
SnapGene 6.0.2
https://www.biofeng.com
FEATURES
Location/Qualifiers
source 1..7104
/mol_type="other
DNA"
/organism="synthetic DNA construct"
oriT 623..732
/note="incP
origin of transfer"
CDS 765..1136
/codon_start=1
/gene="traJ"
/product="oriT-
recognizing protein"
/label=traJ
/translation="MADETKPTRKGSPPIKVYCLPDER
RAIEEKAAAAGMSLSAYLLAV
GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTD
DPRTARFGDATILALLAKIEE
KQDELGKVMMGVVRPRAEP"
rep_origin 1754..2142
/label=R6K gamma
ori
/note="gamma
replication origin from E. coli
plasmid R6K;
requires the R6K
initiator protein pi for replication"
protein_bind 2177..2193
/label=lac
operator
/bound_moiety="lac repressor encoded
by lacI"
/note="The lac
repressor binds to the lac operator to
inhibit
transcription in E. coli. This
inhibition can be
relieved by
adding lactose or
isopropyl-beta-
D-thiogalactopyranoside (IPTG)."
promoter 2574..2676
/label=cat
promoter
/note="promoter
of the E. coli cat gene encoding
chloramphenicol
acetyltransferase"
CDS 2677..3336
/codon_start=1
/gene="cat"
/product="chloramphenicol
acetyltransferase"
/label=CmR
/note="confers
resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEA
FQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELV
IWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENM
FFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHV
GRMLNELQQYCDEWQGGA"
promoter 3753..4198
/gene="sacR"
/label=sacB
promoter
/note="sacB
promoter and control region"
CDS 4199..5620
/codon_start=1
/gene="Bacillus
subtilis sacB"
/product="secreted levansucrase that
renders bacterial
growth sensitive
to sucrose"
/label=SacB
/note="negative
selection marker"
/translation="MNIKKFAKQATVLTFTTALLAGGA
TQAFAKETNQKPYKETYGISH
ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLD
VWDSWPLQNADGTVANYHGYH
IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRV
FKDSDKFDANDSILKDQTQEW
SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSA
SDSSLNINGVEDYKSIFDGDG
KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLV
FEANTGTEDGYQGEESLFNKA
YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELN
DDYTLKKVMKPLIASNTVTDE
IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLG
YVSNSLTGPYKPLNKTGLVLK
MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYA
DKQSTFAPSFLLNIKGKKTSV
VKDSILEQGQLTVNK"
ORIGIN
1 gatccttttt gtccggtgtt
gggttgaagg tgaagccggt cggggccgca
gcgggggccg
61 gcttttcagc cttgcccccc
tgcttcggcc gccgtggctc cggcgtcttg
ggtgccggcg
121 cgggttccgc agccttggcc
tgcggtgcgg gcacatcggc gggcttggcc
ttgatgtgcc
181 gcctggcgtg cgagcggaac
gtctcgtagg agaacttgac cttccccgtt
tcccgcatgt
241 gctcccaaat ggtgacgagc
gcatagccgg acgctaacgc cgcctcgaca
tccgccctca
301 ccgccaggaa cgcaaccgca
gcctcatcac gccggcgctt cttggccgcg
cgggattcaa
361 cccactcggc cagctcgtcg
gtgtagctct ttggcatcgt ctctcgcctg
tcccctcagt
421 tcagtaattt cctgcatttg
cctgtttcca gtcggtagat attccacaaa
acagcaggga
481 agcagcgctt ttccgctgca
taaccctgct tcggggtcat tatagcgatt
ttttcggtat
541 atccatcctt tttcgcacga
tatacaggat tttgccaaag ggttcgtgta
gactttcctt
601 ggtgtatcca acggcgtcag
ccgggcagga taggtgaagt aggcccaccc
gcgagcgggt
661 gttccttctt cactgtccct
tattcgcacc tggcggtgct caacgggaat
cctgctctgc
721 gaggctggcc ggctaccgcc
ggcgtaacag atgagggcaa gcggatggct
gatgaaacca
781 agccaaccag gaagggcagc
ccacctatca aggtgtactg ccttccagac
gaacgaagag
841 cgattgagga aaaggcggcg
gcggccggca tgagcctgtc ggcctacctg
ctggccgtcg
901 gccagggcta caaaatcacg
ggcgtcgtgg actatgagca cgtccgcgag
ctggcccgca
961 tcaatggcga cctgggccgc
ctgggcggcc tgctgaaact ctggctcacc
gacgacccgc
1021 gcacggcgcg gttcggtgat
gccacgatcc tcgccctgct ggcgaagatc
gaagagaagc
1081 aggacgagct tggcaaggtc
atgatgggcg tggtccgccc gagggcagag
ccatgacttt
1141 tttagccgct aaaacggccg
gggggtgcgc gtgattgcca agcacgtccc
catgcgctcc
1201 atcaagaaga gcgacttcgc
ggagctggtg aagtacatca ccgacgagca
aggcaagacc
1261 gagcgcctgg gtcacgtgcg
cgtcacgaac tgcgaggcaa acaccctgcc
cgctgtcatg
1321 gccgaggtga tggcgaccca
gcacggcaac acccgttccg aggccgacaa
gacctatcac
1381 ctgctggtta gcttccgcgc
gggagagaag cccgacgcgg agacgttgcg
cgcgattgag
1441 gaccgcatct gcgctgggct
tggcttcgcc gagcatcagc gcgtcagtgc
cgtgcatcac
1501 gacaccgaca acctgcacat
ccatatcgcc atcaacaaga ttcacccgac
ccgaaacacc
1561 atccatgagc cgtatcgggc
ctaccgcgcc ctcgctgacc tctgcgcgac
gctcgaacgg
1621 gactacgggc ttgagcgtga
caatcacgaa acgcggcagc gcgtttccga
gaaccgcgcg
1681 aacgacatgg agcggcacgc
gggcgtggaa agcctggtcg gctggatccg
gccacgatgc
1741 gtccggcgta gaggatctga
agatcagcag ttcaacctgt tgatagtacg
tactaagctc
1801 tcatgtttca cgtactaagc
tctcatgttt aacgtactaa gctctcatgt
ttaacgaact
1861 aaaccctcat ggctaacgta
ctaagctctc atggctaacg tactaagctc
tcatgtttca
1921 cgtactaagc tctcatgttt
gaacaataaa attaatataa atcagcaact
taaatagcct
1981 ctaaggtttt aagttttata
agaaaaaaaa gaatatataa ggcttttaaa
gcttttaagg
2041 tttaacggtt gtggacaaca
agccagggat gtaacgcact gagaagccct
tagagcctct
2101 caaagcaatt ttgagtgaca
caggaacact taacggctga catgggaatt
ccacatgtgg
2161 aattccacat gtggaattgt
gagcggataa caatttgtgg aatcccggga
gagctcaggt
2221 tacccgcatg caagatctat
ctagaagggc cccactagtg acgcgtactc
gagggtcgac
2281 ggtatcgata agcttgatat
acactccgct agcgctgatg tccggcggtg
cttttgccgt
2341 tacgcaccac cccgtcagta
gctgaacagg agggacagct gatagaaaca
gaagccactg
2401 gagcacctca aaaacaccat
catacactaa atcagtaagt tggcagcatc
acccgacgca
2461 ctttgcgccg aataaatacc
tgtgacggaa gatcacttcg cagaataaat
aaatcctggt
2521 gtccctgttg ataccgggaa
gccctgggcc aacttttggc gaaaatgaga
cgttgatcgg
2581 cacgtaagag gttccaactt
tcaccataat gaaataagat cactaccggg
cgtatttttt
2641 gagttatcga gattttcagg
agctaargaa gctaaaatgg agaaaaaaat
cactggatat
2701 accaccgttg atatatccca
atggcatcgt aaagaacatt ttgaggcatt
tcagtcagtt
2761 gctcaatgta cctataacca
gaccgttcag ctggatatta cggccttttt
aaagaccgta
2821 aagaaaaata agcacaagtt
ttatccggcc tttattcaca ttcttgcccg
cctgatgaat
2881 gctcatccgg aattccgtat
ggcaatgaaa gacggtgagc tggtgatatg
ggatagtgtt
2941 cacccttgtt acaccgtttt
ccatgagcaa actgaaacgt tttcatcgct
ctggagtgaa
3001 taccacgacg atttccggca
gtttctacac atatattcgc aagatgtggc
gtgttacggt
3061 gaaaacctgg cctatttccc
taaagggttt attgagaata tgtttttcgt
ctcagccaat
3121 ccctgggtga gtttcaccag
ttttgattta aacgtggcca atatggacaa
cttcttcgcc
3181 cccgttttca ccatgggcaa
atattatacg caaggcgaca aggtgctgat
gccgctggcg
3241 attcaggttc atcatgccgt
ttgtgatggc ttccatgtcg gcagaatgct
taatgaatta
3301 caacagtact gcgatgagtg
gcagggcggg gcgtaatttt tttaaggcag
ttattggtgc
3361 ccttaaacgc ctggttgcta
cgcctgaata agtgataata agcggatgaa
tggcagaaat
3421 tcgaaagcaa attcgacccg
gtcgtcggtt cagggcaggg tcgttaaata
gccgcttatg
3481 tctattgctg gtttaccggt
ttattgacta ccggaagcag tgtgaccgtg
tgcttctcaa
3541 atgcctgagg ccagwttgct
cagctctccc gtggaggtaa taattgacga
tatgatcatt
3601 tattctgcct cccagagcct
gataaaaacg gttagcgctt cgttaataca
gatgtaggtg
3661 ttccacaggg tagccagcag
catcctgcga tgcagatcat cgaattcctg
cagccaagct
3721 agacctaggc cttaagatcc
tttttaaccc atcacatata cctgccgttc
actattattt
3781 agtgaaatga gatattatga
tattttctga attgtgatta aaaaggcaac
tttatgccca
3841 tgcaacagaa actataaaaa
atacagagaa tgaaaagaaa cagatagatt
ttttagttct
3901 ttaggcccgt agtctgcaaa
tccttttatg attttctatc aaacaaaaga
ggaaaataga
3961 ccagttgcaa tccaaacgag
agtctaatag aatgaggtcg aaaagtaaat
cgcgcgggtt
4021 tgttactgat aaagcaggca
agacctaaaa tgtgtaaagg gcaaagtgta
tactttggcg
4081 tcacccctta catattttag
gtcttttttt attgtgcgta actaacttgc
catcttcaaa
4141 caggagggct ggaagaagca
gaccgctaac acagtacata aaaaaggaga
catgaacgat
4201 gaacatcaaa aagtttgcaa
aacaagcaac agtattaacc tttactaccg
cactgctggc
4261 aggaggcgca actcaagcgt
ttgcgaaaga aacgaaccaa aagccatata
aggaaacata
4321 cggcatttcc catattacac
gccatgatat gctgcaaatc cctgaacagc
aaaaaaatga
4381 aaaatatcaa gttcctgaat
tcgattcgtc cacaattaaa aatatctctt
ctgcaaaagg
4441 cctggacgtt tgggacagct
ggccattaca aaacgctgac ggcactgtcg
caaactatca
4501 cggctaccac atcgtctttg
cattagccgg agatcctaaa aatgcggatg
acacatcgat
4561 ttacatgttc tatcaaaaag
tcggcgaaac ttctattgac agctggaaaa
acgctggccg
4621 cgtctttaaa gacagcgaca
aattcgatgc aaatgattct atcctaaaag
accaaacaca
4681 agaatggtca ggttcagcca
catttacatc tgacggaaaa atccgtttat
tctacactga
4741 tttctccggt aaacattacg
gcaaacaaac actgacaact gcacaagtta
acgtatcagc
4801 atcagacagc tctttgaaca
tcaacggtgt agaggattat aaatcaatct
ttgacggtga
4861 cggaaaaacg tatcaaaatg
tacagcagtt catcgatgaa ggcaactaca
gctcaggcga
4921 caaccatacg ctgagagatc
ctcactacgt agaagataaa ggccacaaat
acttagtatt
4981 tgaagcaaac actggaactg
aagatggcta ccaaggcgaa gaatctttat
ttaacaaagc
5041 atactatggc aaaagcacat
cattcttccg tcaagaaagt caaaaacttc
tgcaaagcga
5101 taaaaaacgc acggctgagt
tagcaaacgg cgctctcggt atgattgagc
taaacgatga
5161 ttacacactg aaaaaagtga
tgaaaccgct gattgcatct aacacagtaa
cagatgaaat
5221 tgaacgcgcg aacgtcttta
aaatgaacgg caaatggtac ctgttcactg
actcccgcgg
5281 atcaaaaatg acgattgacg
gcattacgtc taacgatatt tacatgcttg
gttatgtttc
5341 taattcttta actggcccat
acaagccgct gaacaaaact ggccttgtgt
taaaaatgga
5401 tcttgatcct aacgatgtaa
cctttactta ctcacacttc gctgtacctc
aagcgaaagg
5461 aaacaatgtc gtgattacaa
gctatatgac aaacagagga ttctacgcag
acaaacaatc
5521 aacgtttgcg ccaagcttcc
tgctgaacat caaaggcaag aaaacatctg
ttgtcaaaga
5581 cagcatcctt gaacaaggac
aattaacagt taacaaataa aaacgcaaaa
gaaaatgccg
5641 atatcctatt ggcattttct
tttatttctt atcaacataa aggtgaatcc
catatgaact
5701 atataaaagc aggcaaatgg
ctaaccgtat tcctaacctt ttggtaatga
ctccaactta
5761 ttgatagtgt tttatgttca
gataatgccc gatgactttg tcatgcagct
ccaccgattt
5821 tgagaacgac agcgacttcc
gtcccagccg tgccaggtgc tgcctcagat
tcaggttatg
5881 ccgctcaatt cgctgcgtat
atcgcttgct gattacgtgc agctttccct
tcaggcggga
5941 ttcatacagc ggccagccat
ccgtcatcca tatcaccacg tcaaagggtg
acagcaggct
6001 cataagacgc cccagcgtcg
ccatagtgcg ttcaccgaat acgtgcgcaa
caaccgtctt
6061 ccggagactg tcatacgcgt
aaaacagcca gcgctggcgc gatttagccc
cgacatagcc
6121 ccactgttcg tccatttccg
cgcagacgat gacgtcactg cccggctgta
tgcgcgaggt
6181 taccgactgc ggcctgagtt
ttttaagtga cgtaaaatcg tgttgaggcc
aacgcccata
6241 atgcgggctg ttgcccggca
tccaacgcca ttcatggcca tatcaatgat
tttctggtgc
6301 gtaccgggtt gagaagcggt
gtaagtgaac tgcagcaatg gcaacaacgt
tgcgcaaact
6361 attaactggc gaactactta
ctctagcttc ccggcaacaa ttaatagact
ggatggaggc
6421 ggataaagtt gcaggaccac
ttctgcgctc ggcccttccg gctggct
