pMXs-hSOX2

价格:1200元

联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

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载体基本信息

质粒名称: pMXs-hSOX2 
出品公司: 爱迪基因
目录编号: 17218
存储实验室: Shinya Yamanaka

质粒骨架基本信息
质粒名称: pMXs
质粒抗性: 氨苄霉素
空质粒大小: 4600 bp
质粒类型: 哺乳细胞表达
高拷贝/低拷贝: 高拷贝
生长条件: 37 ℃

插入基因信息
插入基因名称: SOX2
物种来源: H. sapiens (human)
插入大小: 960 bp
基因突变: None
克隆方法: 限制性内切酶
5' 克隆位点: attB1(未破坏)
3' 克隆位点: attB2(未破坏)
5' 测序引物 pMX-S1811
3' 测序引物  

文献引用方法
材料和方法部分: pMXs-hSOX2 was a gift from Shinya Yamanaka (Addgene plasmid # 17218)
参考文献部分: Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S. Cell. 2007 Nov 30. 131(5):861-72. 10.1016/j.cell.2007.11.019 PubMed 18035408

载体图谱质粒图谱和多克隆位点信息

pMXs-hSOX2载体图谱

载体简介

载体序列

LOCUS       pMXs-hSOX2 Sequencing Result	5721 bp 	DNA	SYN
DEFINITION  pMXs-hSOX2 Sequencing Result
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..5721
                     /organism="pMXs-hSOX2 Sequencing Result"
                     /mol_type="other DNA"
     misc_feature    complement(130..636)
                     /label="3MoMuLV_LTR"
     misc_feature    complement(130..594)
                     /label="5_LTR2"
     misc_feature    complement(831..855)
                     /label="attB2"
     CDS             complement(872..1825)
                     /label="ORF frame 3"
     misc_feature    complement(1846..1870)
                     /label="attB1"
     misc_feature    complement(1919..3200)
                     /label="psi_plus_pack2"
     misc_feature    complement(1967..1983)
                     /label="pBMN_5_primer"
     misc_feature    complement(2236..2715)
                     /label="gag"
     misc_feature    complement(2321..2337)
                     /label="pBABE_5_primer"
     misc_feature    complement(2353..2375)
                     /label="pLXSN_5_primer"
     misc_feature    complement(2353..2375)
                     /label="MSCV_primer"
     misc_feature    complement(3200..3751)
                     /label="5_LTR2"
     misc_feature    complement(3200..3793)
                     /label="3MoMuLV_LTR"
     promoter        3858..3886
                     /label="AmpR_promoter"
     gene            3928..4788
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             3928..4788
                     /label="ORF frame 1"
     rep_origin      4936..5555
                     /label="pBR322_origin"
ORIGIN
    1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgCAACTATG
   61 GCCATTTAAT GTAAATACTT AAGAAAAAAA ACCAAATTAA TTTTGATACA TGCTGCATGT
  121 GAAAGACCCC CGCTGACGGG TAGTCAATCA CTCAGAGGAG ACCCTCCCAA GGAACAGCGA
  181 GACCACAAGT CGGATGCAAC TGCAAGAGGG TTTATTGGAT ACACGGGTAC CCGGGCGACT
  241 CAGTCAATCG GAGGACTGGC GCGCCGAGTG AGGGGTTGTG GGCTCTTTTA TTGAGCTCGG
  301 GGAGCAGAAG CGCGCGAACA GAAGCGAGAA GCGAACTGAT TGGTTAGTTC AAATAAGGCA
  361 CAGGGTCATT TCAGGTCCTT GGGGCACCCT GGAAACATCT GATGGTTCTC TAGAAACTGC
  421 TGAGGGCTGG ACCGCATCTG GGGACCATCT GTTCTTGGCC CTGAGCCGGG GCAGGAACTG
  481 CTTACCACAG ATATCCTGTT TGGCCCATAT TCAGCTGTTC CATCTGTTCC TGACCTTGAT
  541 CTGAACTTTT CTATTCTCAG TTATGTATTT TTCCATGCCT TGCAAAATGG CGTTACTTAA
  601 GCTAGCTTGC CAAACCTACA GGTGGGGTCT TTCATTCCCC CCTTTTTCTG GAGACTAAAT
  661 AAAATCTTTT ATTTTATCGT CGACCACTGT GCTGGCGGCC GCTCGAGTTT AAATAGTACA
  721 TAATGGATTT CCTTACGCGA AATACGGGCA GACATGGCCT GCCCGGTTAT TATTATTTTT
  781 GACACCAGAC CAACTGGTAA TGGTAGCGAC CGGCGCTCAG CTGGAATATC ACCACTTTGT
  841 ACAAGAAAGC TGGGTCGGCG CGCCCACCCT TTCACATGTG TGAGAGGGGC AGTGTGCCGT
  901 TAATGGCCGT GCCGGGCACC GGGCCGCTCT GGTAGTGCTG GGACATGTGA AGTCTGCTGG
  961 GGGCGGCGGG TTCCGGCACC TCGGCGCCGG GGAGATACAT GCTGATCATG TCCCGGAGGT
 1021 CCCCGGCCTG GCAGGGCGCC CTGGAGTGGG AGGAAGAGGT AACCACAGGG GGGCTGGAGC
 1081 TGGCCTCGGA CTTGACCACC GAACCCATGG AGCCAAGAGC CATGCCAGGG GTGCCCTGCT
 1141 GCGAGTAGGA CATGCTGTAG GTGGGCGAGC CGTTCATGTA GGTCTGCGAG CTGGTCATGG
 1201 AGTTGTACTG CAGGGCGCTC ACGTCGTAGC GGTGCATGGG CTGCATCTGC GCTGCGCCGT
 1261 GCGCATTGAG GCCCGGGTGC TGCGGGTAGC CCAGCTGGTC CTGCATCATG CTGTAGCTGC
 1321 CGTTGCTCCA GCCGTTCATG TGCGCGTAAC TGTCCATGCG CTGGTTCACG CCCGCGCCCA
 1381 GGCCGGCGCC CACCCCGACC CCGCTCGCCA TGCTATTGCC GCCGGGGGCC AGCAGCCCGC
 1441 CGGGCAGCGT GTACTTATCC TTCTTCATGA GCGTCTTGGT TTTCCGCCGG GGCCGGTATT
 1501 TATAATCCGG GTGCTCCTTC ATGTGCAGCG CTCGCAGCCG CTTAGCCTCG TCGATGAACG
 1561 GCCGCTTCTC CGTCTCCGAC AAAAGTTTCC ACTCGGCGCC CAGGCGCTTG CTGATCTCCG
 1621 AGTTGTGCAT CTTGGGGTTC TCCTGGGCCA TCTTGCGCCG CTGCCCGCGG GACCACACCA
 1681 TGAAGGCATT CATGGGCCGC TTGACGCGGT CCGGGCTGTT TTTCTGGTTG CCGCCGGCCG
 1741 CCGCCGCGGT GGAGTTGCCG CCGCCGCCCC CCGAAGTTTG CTGCGGGCCC GGCGGCTTCA
 1801 GCTCCGTCTC CATCATGTTG TACATGGTGA AGGGGGCGGC CGCGGAGCCT GCTTTTTTGT
 1861 ACAAACTTGT GATTTCCCGT ACCACCACAC TGGGATCCTT AATTAACTAG CTAGATCCGG
 1921 CAGTCTAGAG GATGGTCCAC CCCCGGGGTC GGCAGCCTTC ACGTGGGCGG CGTGTATCCA
 1981 AGCTGCGATG CCGTCTACTT TGAGGGCGGT GGGGGTGGTC AGCAGGACTG TGTAAGGTCC
 2041 TTTCCAGCGA GGTTCTAGGT TCTTAGTCTG GTGTCGGCGG ACCCACACTG TGTCGCCGAC
 2101 TCGGTAAGGG TGAGGTACCA CCGGTCGGTC CAGTTGTTCT TGGTAGGCTG CCGCCAGAGG
 2161 TCTCCAGACT TCGTGCTGGA CTAAGTAGAG AGCCTGTAAG TGAGCTTGGA GAGAGGGGCT
 2221 GTTAGTAACT CTTGTCATGT CAGGGTCAGG GAAGTTTACA AGGGGCGGGG GTGCCCCATA
 2281 TAAGATCTCA TATGGCCATA TGGGGGCGCC TAGAGAAGGA GTGAGGGCTG GATAAAGGGA
 2341 GGATCGAGGC GGGGTCGAAC GAGGAGGTTC AAGGGGGAGA GACGGGGCGG ATGGAGGAAG
 2401 AGGAGGCGGA GGCTTAGGGT GTACAAAGGG CTTGACCCAG GGAGGGGGGT CAAAAGCCAA
 2461 GGCTTCCCAG GTCACGATGT AGGGGACCTG GTCTGGGTGT CCATGCGGGC CAGGTGAAAA
 2521 GACCTTGATC TTAACCTGGG TGATGAGGTC TCGGTTAAAG GTGCCGTCTC GCGGCCATCC
 2581 GACGTTAAAG GTTGGCCATT CTGCAGAGCA GAAGGTAACC CAACGTCTCT TCTTGACATC
 2641 TACCGACTGG TTGTGAGCGA TCCGCTCGAC ATCTTTCCAG TGACCTAAGG TCAAACTTAA
 2701 GGGAGTGGTA ACAGTCTGGC CCTAATTTTC AGACAAATAC AGAAACACAG TCAGACAGAG
 2761 ACAACACAGA ACGATGCTGC AGCAGACAAG ACGCGCGGCG CGGCTTCGGT CCCAAACCGA
 2821 AAGCAAAAAT TCAGACGGAG GCGGGAACTG TTTTAGGTTC TCGTCTCCTA CCAGAACCAC
 2881 ATATCCCTCC TATTAGGGGG GTGCACCAAA GAGTCCAAAA CGATCGGGAT TTTTGGACTC
 2941 AGGTCGGGCC ACAAAAACGG CCCCCGAAGT CCCTGGGACG TCTCCCAGGG TTGCGGCCGG
 3001 GTGTTCCGAA CTCGTCAGTT CCACCACGGG TCCGCCAGAT ACAGAGCTAG TTAGCTAACT
 3061 AGTACCGACG CAGGCGCATA AAATCAGTCA TAGACACTAG ACAATCGGAC AGACACAGAT
 3121 AAGTTGCTGG CCAGCTTACC TCCCGGTGGT GGGTCGGTGG TCCCTGGGCA GGGGTCTCCC
 3181 GATCCCGGAC GAGCCCCCAA ATGAAAGACC CCCGCTGACG GGTAGTCAAT CACTCAGAGG
 3241 AGACCCTCCC AAGGAACAGC GAGACCACAA GTCGGATGCA ACTGCAAGAG GGTTTATTGG
 3301 ATACACGGGT ACCCGGGCGA CTCAGTCAAT CGGAGGACTG GCGCGCCGAG TGAGGGGTTG
 3361 TGGGCTCTTT TATTGAGCTC GGGGAGCAGA AGCGCGCGAA CAGAAGCGAG AAGCGAACTG
 3421 ATTGGTTAGT TCAAATAAGG CACAGGGTCA TTTCAGGTCC TTGGGGCACC CTGGAAACAT
 3481 CTGATGGTTC TCTAGAAACT GCTGAGGGCT GGACCGCATC TGGGGACCAT CTGTTCTTGG
 3541 CCCTGAGCCG GGGCAGGAAC TGCTTACCAC AGATATCCTG TTTGGCCCAT ATTCAGCTGT
 3601 TCCATCTGTT CTTGGCCCTG AGCCGGGGCA GGAACTGCTT ACCACAGATA TCCTGTTTGG
 3661 CCCATATTCA GCTGTTCCAT CTGTTCCTGA CCTTGATCTG AACTTTTCTA TTCTCAGTTA
 3721 TGTATTTTTC CATGCCTTGC AAAATGGCGT TACTTAAGCT AGCTTGCCAA ACCTACAGGT
 3781 GGGGTCTTTC ATTATGCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT
 3841 TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG
 3901 CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT
 3961 CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA
 4021 AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC
 4081 GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA
 4141 GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC
 4201 CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT
 4261 ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT
 4321 GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC
 4381 AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA
 4441 CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA
 4501 TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG
 4561 GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT
 4621 AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT
 4681 AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA
 4741 AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA
 4801 GTTTACTCAT ATATACTTTA GATTGATTTG CGGCCGGCCG CAAAAGGATC TAGGTGAAGA
 4861 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT
 4921 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT
 4981 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC
 5041 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC
 5101 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC
 5161 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG
 5221 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT
 5281 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG
 5341 AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG
 5401 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT
 5461 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG
 5521 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT
 5581 GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA
 5641 TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT
 5701 CAGTGAGCGA GGAAGCGGAA G
//

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