| 质粒名称: | pBK092 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 183146 |
| 存储实验室: | Daan Swarts |
|
载体骨架基本信息 |
|
| 载体名称: | pET-His6-MBP-TEV-LIC cloning vector (1M) |
| 载体出品公司: | Scott Gradia |
| 空载体大小: | 6465 bp |
| 完整质粒大小: | 9391 bp |
| 载体类型: | 细菌表达 |
| 载体抗性: | 卡那霉素50 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | CrtSPARTA operon (6xHis-MBP-CrtTIR-APAZ and CrtAgo),6xHis-MBP-CrtTIR-APAZ,CrtAgo |
| 物种来源: | Crentalea thermophila: DSM 14807 |
| 插入大小: | 4049 bp |
| GenBank编号 | WP_092459739.1 WP_092459742.1 |
| 启动子: | T7 |
| 标签/融合蛋白: |
6xHis（插件上的N端子）
MBP（插入件上的N端子）
|
| 克隆方法: | Gibson克隆 |
| 5’测序引物 | gatgaagccctgaaagacgcgcag |
| 3’测序引物 | ttcgccaatccggatatagttcc |
|
文献引用方法 |
|
| 材料和方法部分: | pBK092 was a gift from Daan Swarts (Addgene plasmid # 183146 ; http://n2t.net/addgene:183146 ; RRID:Addgene_183146) |
| 参考文献部分: | Short prokaryotic Argonaute systems trigger cell death upon detection of invading DNA. Koopal B, Potocnik A, Mutte SK, Aparicio-Maldonado C, Lindhoud S, Vervoort JJM, Brouns SJJ, Swarts DC. Cell, 2022. 10.1016/j.cell.2022.03.012 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pBK092 9388 bp ds-DNA circular SYN 21-APR-2022
DEFINITION pET-6xHis-MBP-TEV-CrtTIR-APAZ/CrtAgo - Heterologous CrtSPARTA
expression.
ACCESSION .
VERSION .
KEYWORDS pBK092
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9388)
AUTHORS Koopal B, Potocnik A, Mutte SK, Aparicio-Maldonado C, Lindhoud S,
Vervoort JJM, Brouns SJJ, Swarts DC
TITLE Short prokaryotic Argonaute systems trigger cell death upon
detection of invading DNA
JOURNAL Cell, 2022.
REFERENCE 2 (bases 1 to 9388)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 21, 2022 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9388
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS complement(319..1401)
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
primer_bind 1363..1382
/label=LacI-R
/note="LacI, reverse primer"
promoter complement(1402..1479)
/gene="lacI"
/label=lacI promoter
primer_bind 1685..1704
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind 1788..1807
/label=T7
/note="T7 promoter, forward primer"
promoter 1788..1806
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 1807..1831
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 1846..1868
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 1887..1904
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
CDS 1914..3014
/codon_start=1
/gene="malE (mutated)"
/product="maltose binding protein from E. coli"
/label=MBP
/note="This version of the gene does not encode a signal
sequence, so MBP will remain in the cytosol."
/translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
WYAVRTAVINAASGRQTVDEALKDAQT"
primer_bind 2988..3011
/label=MBP-F
/note="Maltose binding protein, forward primer"
CDS 3027..3047
/codon_start=1
/product="tobacco etch virus (TEV) protease recognition and
cleavage site"
/label=TEV site
/translation="ENLYFQS"
CDS 6039..6056
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(6109..6127)
/label=T7 Term
/note="T7 terminator, reverse primer"
terminator 6123..6170
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
rep_origin 6207..6662
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6294..6313)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6504..6525
/label=F1ori-F
/note="F1 origin, forward primer"
CDS complement(6755..7570)
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
primer_bind 7478..7497
/label=Kan-R
/note="Kanamycin resistance gene, reverse primer"
primer_bind 7562..7581
/label=pENTR-R
/note="pENTR vectors, reverse primer"
rep_origin 7692..8280
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8181..8200
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 8434..8451
/label=L4440
/note="L4440 vector, forward primer"
misc_feature 8466..8605
/label=bom
/note="basis of mobility region from pBR322"
primer_bind complement(8531..8550)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 8691..8713
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
CDS complement(8707..8898)
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/label=rop
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
ORIGIN
1 gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg
61 tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg
121 tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag
181 aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact
241 gggttgaagg ctctcaaggg catcggtcga gatcccggtg cctaatgagt gagctaactt
301 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
361 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt
421 ttttcttttc accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga
481 gagttgcagc aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt
541 ggttaacggc gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat
601 atccgcacca acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg
661 atcgttggca accagcatcg cagtgggaac gataccctca ttcagcattt gcatggtttg
721 ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt
781 gcgagtgaga tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg
841 gcccgctaac agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg
901 cgtaccgtct tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag
961 aaataacgcc ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag
1021 cggatagtta atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt
1081 acaggcttcg acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc
1141 ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt
1201 ggcaacgcca atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat
1261 gtaattcagc tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct
1321 ggcctggttc accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc
1381 gtataacgtt actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca
1441 tgccataccg cgaaaggttt tgcgccattc gatggtgtcc gggatctcga cgctctccct
1501 tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg
1561 ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg
1621 ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc
1681 catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg
1741 ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaattaa tacgactcac
1801 tataggggaa ttgtgagcgg ataacaattc ccctctagaa ataattttgt ttaactttaa
1861 gaaggagata taccatgggt tcttctcacc atcaccatca ccatggttct tctatgaaaa
1921 tcgaagaagg taaactggta atctggatta acggcgataa aggctataac ggtctcgctg
1981 aagtcggtaa gaaattcgag aaagataccg gaattaaagt caccgttgag catccggata
2041 aactggaaga gaaattccca caggttgcgg caactggcga tggccctgac attatcttct
2101 gggcacacga ccgctttggt ggctacgctc aatctggcct gttggctgaa atcaccccgg
2161 acaaagcgtt ccaggacaag ctgtatccgt ttacctggga tgccgtacgt tacaacggca
2221 agctgattgc ttacccgatc gctgttgaag cgttatcgct gatttataac aaagatctgc
2281 tgccgaaccc gccaaaaacc tgggaagaga tcccggcgct ggataaagaa ctgaaagcga
2341 aaggtaagag cgcgctgatg ttcaacctgc aagaaccgta cttcacctgg ccgctgattg
2401 ctgctgacgg gggttatgcg ttcaagtatg aaaacggcaa gtacgacatt aaagacgtgg
2461 gcgtggataa cgctggcgcg aaagcgggtc tgaccttcct ggttgacctg attaaaaaca
2521 aacacatgaa tgcagacacc gattactcca tcgcagaagc tgcctttaat aaaggcgaaa
2581 cagcgatgac catcaacggc ccgtgggcat ggtccaacat cgacaccagc aaagtgaatt
2641 atggtgtaac ggtactgccg accttcaagg gtcaaccatc caaaccgttc gttggcgtgc
2701 tgagcgcagg tattaacgcc gccagtccga acaaagagct ggcaaaagag ttcctcgaaa
2761 actatctgct gactgatgaa ggtctggaag cggttaataa agacaaaccg ctgggtgccg
2821 tagcgctgaa gtcttacgag gaagagttgg cgaaagatcc acgtattgcc gccactatgg
2881 aaaacgccca gaaaggtgaa atcatgccga acatcccgca gatgtccgct ttctggtatg
2941 ccgtgcgtac tgcggtgatc aacgccgcca gcggtcgtca gactgtcgat gaagccctga
3001 aagacgcgca gactaatggg atcgaggaaa acctgtactt ccaatccaat gcaagaaata
3061 aaattttcat aagccacgcc acaccagagg atgatgattt taccaggtgg ttatcgctaa
3121 aactcattgg tctgggttat gaagtttggt gtgatatcct tttcttggac aagggagttg
3181 acttttggag caccattgaa aaagagatac gggaaaacac ctgtaaattc ctaattgttt
3241 cttccacagc aggaaataag cgggaaggtg tcttgaaaga actggcggta gcaacgaaag
3301 ttaaaaagca ccttcaagat gatatgttca tcataccgct tgctattgat gaaaatctgt
3361 cttatgatga catcaatatc gaaattgtcc gtttaaacgc catcgacttt aaaaaatcct
3421 gggcaaaagg attgcaggac ttgctggacg cattcgaaaa gcagaatgtt ccaaaaaaac
3481 ctcctgacca ttcaaaaagc aatttgcttt accaacaaat tttccttcat gacaagcaag
3541 cgattgagaa ggaagaaacc tatgattcta actggttccc tattatttca tttcctaatg
3601 aactaaggtt tcatagatac gattggcgtt tgccaaaaca atttgatgta agaaccctcg
3661 catttccagc tattcgctac aaagagtatc tgtgcacgtt tgcatgggag tatgatttca
3721 tacaccaatt accaaaaacc gaaacataca atggccagga atcaattaga atttctacaa
3781 gtgatattct atcaggtagg tatgacactg attttatcag aaactacgaa tgtcaaagac
3841 tgattgtcca gcttatcaac aaggcatttg agcttagaat gaaagataag aatgtaaggg
3901 aatatcaaat gtcgaaaacc tttgcttatt ggattgaaaa aggcaaactg gaaaaagaca
3961 aatttgaaaa gattaagtta gtcggcaaac agaaaaataa atactggcac tttgggattt
4021 cggcagccgg taagctctat ccctctcccg ttttaatggt ttcgagtcat attatcttta
4081 ccatggatgg tattaatctt atcaaatcta aaagcattca gcattcatct cgaagaaaac
4141 agggtaagaa ttggtggaat gacaaatggc gggaaaaatt acttgccttt atccgatttc
4201 tttcagatga tcaaaacgct atttacctaa atgtaggaag cgaagagaaa atcctgattt
4261 caaataaacc tctgaaattt tttgggaaaa tgagttatgt aacgccttct gaagtaactt
4321 tggaagaaga atcagtatta gcagatataa acaactttga agaagatacg gaagatttag
4381 acgaattaga agatattgaa taactcgaag cttagatttt aaataacaat actaaggagg
4441 aattacatat gaaggaattg atctacatag aagaaccctc tatcctcttt gctcatggtc
4501 agaaatgtac tgatcctaga gatggattgg cattattcgg acctctgaat cagatatatg
4561 gtattaaaag tggagtcgtt ggaacacaaa aaggtttgca gattttcaaa tcctatctgg
4621 ataaaattca gaaaccaata tacaaccata acaatattac aagacccatg ttcccgggtt
4681 ttgaagcagt ttttggctgc aagtgggaat ctcaaaacat tgtattcaaa gaaataactg
4741 acgaagaaat tagaagatat ttgtttaatg ccagcacaca taaacggacg tatgatttgg
4801 taacgctttt taatgacaaa attattaccg ccaataaaaa tgatgaagaa cgagttgatg
4861 tttggtttgt cattgttcct gaagaaattt acaagtattg ccggcccaat tcagttcttc
4921 caaatgagtt ggtccagacg aaatctctaa tctcgaaatc gaaagcaaag tcgtttcgat
4981 atacaccaac gctattcgaa gagttcaaca agaaactgaa agaggtagag aaagaagcaa
5041 aaacctataa ctacgatgca cagtttcacg atcagctaaa agctcgatta ctggaacata
5101 cgattccaac tcaaatactg agagaaagca cattagcatg gagggatttt aagaacacct
5161 ttggtgctcc aataagggat tttagtaaga ttgaaggtca tctggcctgg acaatttcaa
5221 ctgctgcgta ctacaaagcc ggtggcaaac catggaaact tggtgacatc cgacccggag
5281 tatgctattt aggattagtt tacaaaaaaa tagagaaaag taaaaatccc caaaatgcgt
5341 gttgcgcagc tcaaatgttc ttggataacg gtgatggcac tgtctttaaa ggtgaagttg
5401 gcccttggta caaccctgaa aagggcgaat atcatttgaa gcccaaggag gctaaagctc
5461 ttttgactca agcattagaa tcgtacaagg aacaaaacaa aagctacccg aaagaagtat
5521 tcatacatgc cagaactcgc tttaatgatg aggagtggaa tgcatttaat gaagtaaccc
5581 caaaaaatac aaaccttgtt ggggtgacaa ttactaaatc aaaaccatta aaattataca
5641 agacagaagg agcttttcca ataatgagag ggaatgcata tatagtggat gaaaaaaaag
5701 cgtttttatg gacattaggt tttgttccta aactgcaatc tactctttca atggaagttc
5761 cgaatcctat tttcattgaa ataaataaag gagaagccga aattcaacaa gtattgaagg
5821 acatacttgc attaacaaaa ttgaattaca atgcttgtat ttatgcagac ggagaaccag
5881 tgacacttcg ttttgcgaat aaaatcggag aaatacttac ggccagtaca gaaataaaaa
5941 caccaccact tgcatttaaa tactacatat aataacattg gaagtggata acggatccga
6001 attcgagcgc cgtcgacaag cttgcggccg cactcgagca ccaccaccac caccactgag
6061 atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat
6121 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag
6181 gaactatatc cggattggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
6241 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
6301 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
6361 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
6421 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
6481 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
6541 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
6601 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaactagtaa cgtttacaat
6661 ttcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
6721 acattcaaat atgtatccgc tcatgaatta attcttagaa aaactcatcg agcatcaaat
6781 gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa agccgtttct
6841 gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt
6901 ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa
6961 ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt
7021 tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac
7081 tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga aatacgcgat
7141 cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca
7201 gcgcatcaac aatgttttca cctgaatcag gatattcttc taatacctgg aatgctgttt
7261 tcccggggat cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga
7321 tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat
7381 cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat
7441 acaatcgata gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat
7501 ataaatcagc atccatgttg gaatttaatc gcggcctaga gcaagacgtt tcccgttgaa
7561 tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg
7621 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
7681 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
7741 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
7801 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
7861 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
7921 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
7981 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
8041 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
8101 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
8161 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
8221 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
8281 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
8341 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
8401 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
8461 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
8521 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
8581 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
8641 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
8701 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
8761 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
8821 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
8881 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat
8941 gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg
9001 gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa
9061 aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag
9121 ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt
9181 ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga
9241 cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc
9301 agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac
9361 ccgtggggcc gccatgccgg cgataatg
//
