pIEx/Bac-3

价格:1700元

联系方式:I47-825O-882O

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pIEx-Bac-3载体基本信息

出品公司: Novagen
载体名称: pIEx/Bac-3
质粒类型: 昆虫细胞表达载体;蛋白纯化
高拷贝/低拷贝: 高拷贝
启动子: IE-1
克隆方法: 多克隆位点,限制性内切酶
载体大小: 6802 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: N-His tag, C-Strep tagII
载体抗性: 氨苄青霉素(Ampicillin)
筛选标记: --
备注: 具有两套标签,可以采用两套纯化方案进行蛋白纯化。
产品目录号: #71726
稳定性: --
组成型: --
病毒/非病毒: 杆状病毒

pIEx-Bac-3载体质粒图谱和多克隆位点信息

pIEx-Bac-3载体图谱



pIEx-Bac-3多克隆位点

pIEx-Bac-3载体简介

The pIEx/Bac-3 vectors are dual-purpose vectors that offer unique flexibility for cloning and expression of proteins in 

Spodoptera-derived insect cells. These vectors contain the Autographa californica nuclear polyhedrosis virus (AcNPV) 

enhancer/immediate early promoter combination (hr5/ie1) for plasmid-mediated and early baculovirus expression, and the AcNPV 

p10 very late promoter for robust baculovirus-mediated expression. All pIEx/Bac plasmids carry AcNPV-derived virus regions 

to mediate recombination at the polh locus. The pIEx/Bac-3 vector contains an N-terminal His•Tag® coding sequence with 10 

histidines followed by an HRV 3C protease cleavage site and a multiple cloning site, with the option to include a C-terminal 

thrombin protease cleavage site and 8 aa Strep•Tag® II coding sequence. pIEx/Bac-3 is also available as a 3C/LIC vector. The 

3C/LIC vector is prepared for rapid directional cloning of PCR-amplified DNA. Using specifically designed primers for 

amplification, inserts can be efficiently cloned without the need for restriction enzyme digestion or ligation.

pIEx-Bac-3载体序列

LOCUS       pIEx/Bac-3   6802 bp    DNA   circular            17-OCT-2006
DEFINITION  pIEx/BAC/p10 10xHis/3cLIC/Tb/Strep.
SOURCE      
  ORGANISM  
FEATURES             Location/Qualifiers
     misc_recomb     complement(2922..4340)
                     /dnas_title="1629"
                     /vntifkey="86"
                     /label=1629
     rep_origin      6277..6732
                     /dnas_title="f1 origin"
                     /vntifkey="33"
                     /label=f1 origin
     CDS             complement(5288..6145)
                     /dnas_title="Ap"
                     /vntifkey="4"
                     /label=Ap
     rep_origin      complement(4527..4527)
                     /dnas_title="origin"
                     /vntifkey="33"
                     /label=origin
     misc_recomb     4..453
                     /dnas_title="lef-2"
                     /vntifkey="86"
                     /label=lef-2
     misc_recomb     complement(491..1096)
                     /dnas_title="ORF 603"
                     /vntifkey="86"
                     /label=ORF 603
     rep_origin      4360..5119
                     /dnas_title="pUC Ori"
                     /vntifkey="33"
                     /label=pUC Ori
     enhancer        1196..1677
                     /dnas_title="hr5"
                     /vntifkey="9"
                     /label=hr5
     polyA_signal    2748..2753
                     /dnas_title="PolyA signal"
                     /vntifkey="25"
                     /label=PolyA signal
     5'UTR           2229..2278
                     /dnas_title="ie1 5'-UTR"
                     /vntifkey="52"
                     /label=ie1 5'-UTR
     promoter        2279..2391
                     /dnas_title="p10 promoter + 5'UTR"
                     /vntifkey="29"
                     /label=p10 promoter + 5'UTR
     misc_feature    2277..2280
                     /dnas_title="Bsa I jct"
                     /vntifkey="21"
                     /label=Bsa I jct
     promoter        1682..2228
                     /dnas_title="ie1 promoter"
                     /vntifkey="29"
                     /label=ie1 promoter
     terminator      2571..2877
                     /dnas_title="ie1 Terminator"
                     /vntifkey="43"
                     /label=ie1 Terminator
     protein_bind    2529..2552
                     /dnas_title="Strep-Tag II"
                     /vntifkey="31"
                     /label=Strep-Tag II
     protein_bind    2436..2458
                     /dnas_title="3c"
                     /vntifkey="31"
                     /label=3c
     misc_feature    2505..2521
                     /dnas_title="Thrombin site"
                     /vntifkey="21"
                     /label=Thrombin site
     misc_binding    2400..2429
                     /dnas_title="10x His-Tag"
                     /vntifkey="20"
                     /label=10x His-Tag
BASE COUNT     1822 a   1464 c   1508 g   2008 t
ORIGIN      
          1 ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag
         61 agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac
        121 aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat
        181 gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg
        241 gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac
        301 cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat
        361 aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac
        421 tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat
        481 ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa
        541 ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac
        601 agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt
        661 cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt
        721 atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt
        781 ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat
        841 tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt
        901 tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa
        961 ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt
       1021 taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt
       1081 taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta
       1141 tcaatatata gttgctgatc agatctgatc atggagataa ttaaaatgat aagggcgcgt
       1201 aaaacacaat caagtatgag tcataagctg atgtcatgtt ttgcacacgg ctcataaccg
       1261 aactggcttt acgagtagaa ttctacttgt aacgcacgat cagtggatga tgtcatttgt
       1321 ttttcaaatc gagatgatgt catgttttgc acacggctca taaactcgct ttacgagtag
       1381 aattctacgt gtaacgcacg atcgattgat gagtcatttg ttttgcaata tgatatcata
       1441 caatatgact catttgtttt tcaaaaccga acttgattta cgggtagaat tctacttgta
       1501 aagcacaatc aaaaagatga tgtcatttgt ttttcaaaac tgaactcgct ttacgagtag
       1561 aattctacgt gtaaaacaca atcaagaaat gatgtcattt gttataaaaa taaaagctga
       1621 tgtcatgttt tgcacatggc tcataactaa actcgcttta cgggtagaat tctacgcgcg
       1681 tcgatgtctt tgtgatgcgc gcgacatttt tgtaggttat tgataaaatg aacggatacg
       1741 ttgcccgaca ttatcattaa atccttggcg tagaatttgt cgggtccatt gtccgtgtgc
       1801 gctagcatgc ccgtaacgga cctcgtactt ttggcttcaa aggttttgcg cacagacaaa
       1861 atgtgccaca cttgcagctc tgcatgtgtg cgcgttacca caaatcccaa cggcgcagtg
       1921 tacttgttgt atgcaaataa atctcgataa aggcgcggcg cgcgaatgca gctgatcacg
       1981 tacgtccctc gtgttccgtt caaggacggt gttatcgacc tcagattaat gtttatcggc
       2041 cgactgtttt cgtatccgct caccaaacgc gtttttgcat taacattgta tgtcggcgga
       2101 tgttctatat ctaatttgaa taaataaacg ataaccgcgt tggttttaga gggcataata
       2161 aaagaaatat tgttatcgtg ttcgccatta gggcagtata aattgacgtt catgttggat
       2221 attgtttcag ttgcaagttg acactggcgg cgacaagatc gtgaacaacc aagtgactcc
       2281 gggaccttta attcaaccca acacaatata ttatagttaa ataagaatta ttatcaaatc
       2341 atttgtatat taattaaaat actatactgt aaattacatt ttatttacaa tccatggcac
       2401 atcaccacca tcatcaccat caccaccacg gtgcacttga agtcctcttt cagggacccg
       2461 ggtaccagga tcctgtacaa gtcgacgcgg ccgcagagct cgctctggtg ccacgcggta
       2521 gttccgcttg gagccacccg cagttcgaaa agtaagtgat taacctcagg ttatacatat
       2581 attttgaatt taattaatta tacatatatt ttatattatt tttgtctttt attatcgagg
       2641 ggccgttgtt ggtgtggggt tttgcataga aataacaatg ggagttggcg acgttgctgc
       2701 gccaacacca cctcccttcc ctcctttcat catgtatctg tagataaaat aaaatattaa
       2761 acctaaaaac aagaccgcgc ctatcaacaa aatgataggc attaacttgc cgctgacgct
       2821 gtcactaacg ttggacgatt tgccgactaa accttcatcg cccagtaacc aatctaggta
       2881 gctgagcgca tgcaagctga tccgggttat tagtacattt attaagcgct agattctgtg
       2941 cgttgttgat ttacagacaa ttgttgtacg tattttaata attcattaaa tttataatct
       3001 ttagggtggt atgttagagc gaaaatcaaa tgattttcag cgtctttata tctgaattta
       3061 aatattaaat cctcaataga tttgtaaaat aggtttcgat tagtttcaaa caagggttgt
       3121 ttttccgaac cgatggctgg actatctaat ggattttcgc tcaacgccac aaaacttgcc
       3181 aaatcttgta gcagcaatct agctttgtcg atattcgttt gtgttttgtt ttgtaataaa
       3241 ggttcgacgt cgttcaaaat attatgcgct tttgtatttc tttcatcact gtcgttagtg
       3301 tacaattgac tcgacgtaaa cacgttaaat agagcttgga catatttaac atcgggcgtg
       3361 ttagctttat taggccgatt atcgtcgtcg tcccaaccct cgtcgttaga agttgcttcc
       3421 gaagacgatt ttgccatagc cacacgacgc ctattaattg tgtcggctaa cacgtccgcg
       3481 atcaaatttg tagttgagct ttttggaatt atttctgatt gcgggcgttt ttgggcgggt
       3541 ttcaatctaa ctgtgcccga ttttaattca gacaacacgt tagaaagcga tggtgcaggc
       3601 ggtggtaaca tttcagacgg caaatctact aatggcggcg gtggtggagc tgatgataaa
       3661 tctaccatcg gtggaggcgc aggcggggct ggcggcggag gcggaggcgg aggtggtggc
       3721 ggtgatgcag acggcggttt aggctcaaat gtctctttag gcaacacagt cggcacctca
       3781 actattgtac tggtttcggg cgccgttttt ggtttgaccg gtctgagacg agtgcgattt
       3841 ttttcgtttc taatagcttc caacaattgt tgtctgtcgt ctaaaggtgc agcgggttga
       3901 ggttccgtcg gcattggtgg agcgggcggc aattcagaca tcgatggtgg tggtggtggt
       3961 ggaggcgctg gaatgttagg cacgggagaa ggtggtggcg gcggtgccgc cggtataatt
       4021 tgttctggtt tagtttgttc gcgcacgatt gtgggcaccg gcgcaggcgc cgctggctgc
       4081 acaacggaag gtcgtctgct tcgaggcagc gcttggggtg gtggcaattc aatattataa
       4141 ttggaataca aatcgtaaaa atctgctata agcattgtaa tttcgctatc gtttaccgtg
       4201 ccgatattta acaaccgctc aatgtaagca attgtattgt aaagagattg tctcaagctc
       4261 ggatcgatcc cgcacgccga taacaagcct tttcattttt actacagcat tgtagtggcg
       4321 agacacttcg ctgtcgtcga ggtttaaacg cttcctcgct cactgactcg ctgcgctcgg
       4381 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
       4441 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
       4501 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
       4561 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
       4621 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
       4681 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
       4741 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
       4801 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
       4861 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
       4921 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
       4981 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
       5041 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
       5101 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
       5161 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
       5221 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
       5281 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
       5341 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
       5401 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
       5461 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
       5521 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
       5581 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
       5641 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
       5701 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
       5761 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
       5821 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
       5881 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
       5941 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
       6001 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
       6061 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
       6121 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
       6181 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
       6241 gggttccgcg cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa
       6301 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
       6361 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
       6421 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
       6481 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
       6541 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
       6601 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
       6661 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
       6721 cgtttacaat ttcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
       6781 gggcctcttc gctattacgc ca
//

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