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pAAV-TRE-CAG-Fluc载体质粒基本信息
pAAV-TRE-CAG-Fluc质粒图谱载体图谱和pAAV-TRE-CAG-Fluc载体序列质粒序列多克隆位点信息
pAAV-TRE-CAG-Fluc质粒载体简介
pAAV-TRE-CAG-Fluc质粒序列载体序列
LOCUS KC152482 8687 bp DNA circular SYN 03-JUN-2013
DEFINITION Expression vector pAAV-TRE-CAG-Fluc, complete sequence.
ACCESSION KC152482
VERSION KC152482.1 GI:507593971
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pAAV-TRE-CAG-Fluc|
PUBMED 23571608
REMARK Publication Status: Online-Only
REFERENCE 2 (bases 1 to 8687)
AUTHORS Chen,Y. and Sprengel,R.
JOURNAL Submitted (08-NOV-2012) Molecular Neurobiology, Max Planck
institute for medical research, Jahnstr. 29, Heidelberg, BW 69120,
Germany
FEATURES Location/Qualifiers
source 1..8687
/organism="Expression vector pAAV-TRE-CAG-Fluc"
/mol_type="other DNA"
/db_xref="taxon:1337869"
repeat_region 19..162
/note="AAV2 ITR"
/rpt_type=inverted
/rpt_type=terminal
misc_feature 245..542
/note="tetracycline responsive element: TRE"
regulatory 543..559
/regulatory_class="promoter"
/note="CMVmin"
regulatory 564..1504
/regulatory_class="promoter"
/note="CMV earlyenhancer/chicken beta actin promoter: CAG"
misc_feature 1521..3173
/note="firefly luciferase"
misc_feature 3174..3227
/note="2A peptide from Thosea asigna virus"
misc_feature 3240..4088
/note="tTSKid"
misc_feature 3240..3872
/note="Tet repressor: tetR[B/E]"
misc_feature 3873..3890
/note="nuclear localization signal: NLS"
misc_feature 3897..4082
/note="KRAB (Kruppel-associated box) domain; from Rat zinc
finger protein (kid-1)"
misc_feature 4089..4142
/note="2A peptide from Thosea asigna virus"
gene 4155..4811
/gene="mKO"
CDS 4155..4811
/gene="mKO"
/note="fluorescent protein"
/codon_start=1
/transl_table=11
/product="monomeric Kusabira-orange"
/protein_id="AGM53875.1"
/db_xref="GI:507593972"
/translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLR
VTMAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGG
SASVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDV
TMYLKLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS
"
ncRNA 4842..5438
/ncRNA_class="other"
/product="post-transcriptional regulatory element: WPRE"
misc_feature 5471..5681
/note="bovine growth hormone polyA signal: bGH pA"
repeat_region complement(5691..5834)
/note="AAV2 ITR"
/rpt_type=inverted
/rpt_type=terminal
gene 7023..7685
/gene="Bla"
CDS 7023..7685
/gene="Bla"
/note="ampicillin resistance"
/codon_start=1
/transl_table=11
/product="beta-lactamase"
/protein_id="AGM53876.1"
/db_xref="GI:507593973"
/translation="MSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKH
LTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELN
EAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAG
WFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIK
HW"
ORIGIN
1 gggggggggg ggggggggtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
61 gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga
121 gcgcgcagag agggagtggc caactccatc actaggggtt cctagatcta ggatcacgcg
181 ttctagtcct agggatatct tcgaaccatg gttaattaag tttaaacatc gatcaattcg
241 agctcgactt tcacttttct ctatcactga tagggagtgg taaactcgac tttcactttt
301 ctctatcact gatagggagt ggtaaactcg actttcactt ttctctatca ctgataggga
361 gtggtaaact cgactttcac ttttctctat cactgatagg gagtggtaaa ctcgactttc
421 acttttctct atcactgata gggagtggta aactcgactt tcacttttct ctatcactga
481 tagggagtgg taaactcgac tttcactttt ctctatcact gatagggagt ggtaaactcg
541 acctatataa gcagagctcg gtaccattga cgtcaataat gacgtatgtt cccatagtaa
601 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
661 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
721 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
781 acatctacgt attagtcatc gctattacca tggtcgaggt gagccccacg ttctgcttca
841 ctctccccat ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat
901 tttgtgcagc gatgggggcg gggggggggg gggggcgcgc gccaggcggg gcggggcggg
961 gcgaggggcg gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc
1021 tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg
1081 cgcggcgggc gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct
1141 cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg
1201 gcccttctcc tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt
1261 ggctgcgtga aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg
1321 ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc
1381 tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc
1441 ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattg
1501 gatctagtcg attcgaattc atggaagacg ccaaaaacat aaagaaaggc ccggcgccat
1561 tctatccgct agaggatgga accgctggag agcaactgca taaggctatg aagagatacg
1621 ccctggttcc tggaacaatt gcttttacag atgcacatat cgaggtgaac atcacgtacg
1681 cggaatactt cgaaatgtcc gttcggttgg cagaagctat gaaacgatat gggctgaata
1741 caaatcacag aatcgtcgta tgcagtgaaa actctcttca attctttatg ccggtgttgg
1801 gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga catttataat gaacgtgaat
1861 tgctcaacag tatgaacatt tcgcagccta ccgtagtgtt tgtttccaaa aaggggttgc
1921 aaaaaatttt gaacgtgcaa aaaaaattac caataatcca gaaaattatt atcatggatt
1981 ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt cgtcacatct catctacctc
2041 ccggttttaa tgaatacgat tttgtaccag agtcctttga tcgtgacaaa acaattgcac
2101 tgataatgaa ctcctctgga tctactgggt tacctaaggg tgtggccctt ccgcatagaa
2161 ctgcctgcgt cagattctcg catgccagag atcctatttt tggcaatcaa atcattccgg
2221 atactgcgat tttaagtgtt gttccattcc atcacggttt tggaatgttt actacactcg
2281 gatatttgat atgtggattt cgagtcgtct taatgtatag atttgaagaa gagctgtttt
2341 tacgatccct tcaggattac aaaattcaaa gtgcgttgct agtaccaacc ctattttcat
2401 tcttcgccaa aagcactctg attgacaaat acgatttatc taatttacac gaaattgctt
2461 ctgggggcgc acctctttcg aaagaagtcg gggaagcggt tgcaaaacgc ttccatcttc
2521 cagggatacg acaaggatat gggctcactg agactacatc agctattctg attacacccg
2581 agggggatga taaaccgggc gcggtcggta aagttgttcc attttttgaa gcgaaggttg
2641 tggatctgga taccgggaaa acgctgggcg ttaatcagag aggcgaatta tgtgtcagag
2701 gacctatgat tatgtccggt tatgtaaaca atccggaagc gaccaacgcc ttgattgaca
2761 aggatggatg gctacattct ggagacatag cttactggga cgaagacgaa cacttcttca
2821 tagttgaccg cttgaagtct ttaattaaat acaaaggata ccaggtggcc cccgctgaat
2881 tggagtcgat attgttacaa caccccaaca tcttcgacgc gggcgtggca ggtcttcccg
2941 acgatgacgc cggtgaactt cccgccgccg ttgttgtttt ggagcacgga aagacgatga
3001 cggaaaaaga gatcgtggat tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg
3061 gaggagttgt gtttgtggac gaagtaccga aaggtcttac cggaaaactc gacgcaagaa
3121 aaatcagaga gatcctcata aaggccaaga agggcggaaa gtccaaattg ctcgagggca
3181 gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctgca ccgggatcca
3241 tgtccagatt agataaaagt aaagtgatta acagcgcatt agagctgctt aatgaggtcg
3301 gaatcgaagg tttaacaacc cgtaaactcg cccagaagct aggtgtagag cagcctacat
3361 tgtattggca cgtgcgcaac aagcagactc ttatgaacat gctttcagag gcaatactgg
3421 cgaagcatca cacccgttca gcaccgttac cgactgagag ttggcagcag tttctccagg
3481 aaaatgctct gagtttccgt aaagcattac tggtccatcg tgatggagcc cgattgcata
3541 tagggacctc tcctacgccc ccccagtttg aacaagcaga ggcgcaacta cgctgtctat
3601 gcgatgcagg gttttcggtc gaggaggctc ttttcattct gcaatctatc agccatttta
3661 cgttgggtgc agtattagag gagcaagcaa caaaccagat agaaaataat catgtgatag
3721 acgctgcacc accattatta caagaggcat ttaatattca ggcgagaacc tctgctgaaa
3781 tggccttcca tttcgggctg aaatcattaa tatttggatt ttctgcacag ttagatgaaa
3841 aaaagcatac acccattgag gatggtaata aaccaaaaaa gaagagaaag ctagcagtgt
3901 cagtgacatt tgaagatgtg gctgtgctct ttactcggga cgagtggaag aagctggatc
3961 tgtctcagag aagcctgtac cgtgaggtga tgctggagaa ttacagcaac ctggcctcca
4021 tggcaggatt cctgtttacc aaaccaaagg tgatctccct gttgcagcaa ggagaggacc
4081 cgtatctcga gggcagagga agtcttctaa catgcggtga cgtggaggag aatcccggcc
4141 ctgcaccggg atccatggtg agtgtgatta aaccagaaat gaagatgagg tactacatgg
4201 acggctccgt caatgggcat gagttcacaa ttgaaggtga aggcacaggc agaccttacg
4261 agggacatca agagatgaca ctacgcgtca caatggccaa gggcgggcca atgcctttcg
4321 cgtttgactt agtgtcacac gtgttctgtt acggccacag accttttact aaatatccag
4381 aagagatacc agactatttc aaacaagcat ttcctgaagg cctgtcatgg gaaaggtcgt
4441 tggagttcga agatggtggg tccgcttcag tcagtgcgca tataagcctt agaggaaaca
4501 ccttctacca caaatccaaa tttactgggg ttaactttcc tgccgatggt cctatcatgc
4561 aaaaccaaag tgttgattgg gagccatcaa ccgagaaaat tactgccagc gacggagttc
4621 tgaagggtga tgttacgatg tacctaaaac ttgaaggagg cggcaatcac aaatgccaat
4681 tcaagactac ttacaaggcg gcaaaaaaga ttcttaaaat gccaggaagc cattacatca
4741 gccatcgcct cgtcaggaaa accgaaggca acattactga gctggtagaa gatgcagtag
4801 ctcattcctg atgtacaagt aaagcggccg caagcttatc gataatcaac ctctggatta
4861 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
4921 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
4981 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
5041 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
5101 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
5161 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
5221 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg
5281 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
5341 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
5401 gagtcggatc tccctttggg ccgcctcccc gcctgatcga taccgtcgac tagagctcgc
5461 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
5521 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
5581 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
5641 aagggggagg attgggaaga caatagcagg catgctgggg agagatctga ggaaccccta
5701 gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgcccgggca
5761 aagcccgggc gtcgggcgac ctttggtcgc ccggcctcag tgagcgagcg agcgcgcaga
5821 gagggagtgg ccaacccccc cccccccccc cctgcagcct ggcgtaatag cgaagaggcc
5881 cgcaccgatc gcccttccca acagttgcgt agcctgaatg gcgaatggcg cgacgcgccc
5941 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
6001 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
6061 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
6121 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
6181 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
6241 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
6301 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
6361 tttaacaaaa tattaacgtt tacaatttcc tgatgcggta ttttctcctt acgcatctgt
6421 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
6481 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
6541 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
6601 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
6661 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
6721 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
6781 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
6841 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
6901 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
6961 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
7021 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
7081 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
7141 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
7201 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
7261 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
7321 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
7381 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
7441 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
7501 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
7561 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
7621 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
7681 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
7741 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
7801 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
7861 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
7921 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
7981 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
8041 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
8101 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
8161 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
8221 ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa
8281 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
8341 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
8401 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
8461 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
8521 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
8581 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
8641 aaccgcctct ccccgcgcgt tggccgattc attaatgcag gctgcag
//
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