| 出品公司: | Novagen |
|---|---|
| 载体名称: | pIEX-1,pIEX1 |
| 质粒类型: | 昆虫细胞表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | -- |
| 启动子: | -- |
| 载体大小: | 3897 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | -- |
买家导航
pIEX1载体基本信息
pIEX1载体图谱质粒图谱和多克隆位点信息
pIEX1载体简介
pIEX1载体序列
LOCUS pIEx-1 3897 bp ds-DNA circular SYN 25-4-2014
DEFINITION Vector for high-level expression in insect cells of proteins with a
cleavable N-terminal 10xHis-S-Tag cassette plus a C-terminal HSV
tag.
ACCESSION .
VERSION .
KEYWORDS pIEx-1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3897)
AUTHORS Novagen (EMD Millipore)
TITLE Direct Submission
JOURNAL Exported 2017/2/28 from SnapGene 2.3.2
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..3897
/organism="synthetic DNA construct"
/lab_host="Insect Cells"
/mol_type="other DNA"
enhancer 1..483
/gene="Autographa californica hr5"
/note="hr5 enhancer"
/note="baculovirus early transcription enhancer"
promoter 487..1078
/note="IE1 promoter"
/note="promoter of the ie1 gene from the baculovirus
Autographa californica"
CDS 1084..1086
/codon_start=1
/product="start codon"
/note="ATG"
/translation="M"
CDS 1090..1119
/codon_start=1
/product="10xHis affinity tag"
/note="10xHis"
/translation="HHHHHHHHHH"
CDS 1129..1173
/codon_start=1
/product="affinity and epitope tag derived from pancreatic
ribonuclease A"
/note="S-Tag"
/translation="KETAAAKFERQHMDS"
CDS 1189..1206
/codon_start=1
/product="thrombin recognition and cleavage site"
/note="thrombin site"
/translation="LVPRGS"
CDS 1225..1239
/codon_start=1
/product="enterokinase recognition and cleavage site"
/note="enterokinase site"
/translation="DDDDK"
misc_feature 1234..1348
/note="MCS"
/note="multiple cloning site"
CDS 1354..1386
/codon_start=1
/product="HSV (herpes simplex virus) epitope tag"
/note="HSV tag"
/translation="QPELAPEDPED"
terminator 1414..1721
/note="IE1 terminator"
/note="terminator of the ie1 gene from the baculovirus
Autographa californica"
promoter 1751..1855
/gene="bla"
/note="AmpR promoter"
CDS 1856..2716
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 2887..3475
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cgcgtaaaac acaatcaagt atgagtcata agctgatgtc atgttttgca cacggctcat
61 aaccgaactg gctttacgag tagaattcta cttgtaacgc acgatcagtg gatgatgtca
121 tttgtttttc aaatcgagat gatgtcatgt tttgcacacg gctcataaac tcgctttacg
181 agtagaattc tacgtgtaac gcacgatcga ttgatgagtc atttgttttg caatatgata
241 tcatacaata tgactcattt gtttttcaaa accgaacttg atttacgggt agaattctac
301 ttgtaaagca caatcaaaaa gatgatgtca tttgtttttc aaaactgaac tcgctttacg
361 agtagaattc tacgtgtaaa acacaatcaa gaaatgatgt catttgttat aaaaataaaa
421 gctgatgtca tgttttgcac atggctcata actaaactcg ctttacgggt agaattctac
481 gcgcgtcgat gtctttgtga tgcgcgcgac atttttgtag gttattgata aaatgaacgg
541 atacgttgcc cgacattatc attaaatcct tggcgtagaa tttgtcgggt ccattgtccg
601 tgtgcgctag catgcccgta acggacctcg tacttttggc ttcaaaggtt ttgcgcacag
661 acaaaatgtg ccacacttgc agctctgcat gtgtgcgcgt taccacaaat cccaacggcg
721 cagtgtactt gttgtatgca aataaatctc gataaaggcg cggcgcgcga atgcagctga
781 tcacgtacgt ccctcgtgtt ccgttcaagg acggtgttat cgacctcaga ttaatgttta
841 tcggccgact gttttcgtat ccgctcacca aacgcgtttt tgcattaaca ttgtatgtcg
901 gcggatgttc tatatctaat ttgaataaat aaacgataac cgcgttggtt ttagagggca
961 taataaaaga aatattgtta tcgtgttcgc cattagggca gtataaattg acgttcatgt
1021 tggatattgt ttcagttgca agttgacact ggcggcgaca agatcgtgaa caaccaagtg
1081 accatggcac accaccatca ccaccatcac caccatcact cttctggtaa agaaaccgct
1141 gctgcgaaat ttgaacgcca gcacatggac tcgccaccgc cttctggtct ggtcccccgg
1201 ggcagcgcag gttctggtac gattgatgac gacgacaaga gtccgggctt ctcctcaacg
1261 gatcccaatt gggagctcgt gtacacggcg cgcctgcagg tcgacggtac cggttcgaag
1321 cttgcggccg cacagctgta tacacgtgca agccagccag aactcgcccc ggaagacccc
1381 gaggatctcg agcactaagt gattaacctc aggttataca tatattttga atttaattaa
1441 ttatacatat attttatatt atttttgtct tttattatcg aggggccgtt gttggtgtgg
1501 ggttttgcat agaaataaca atgggagttg gcgacgttgc tgcgccaaca ccacctccct
1561 tccctccttt catcatgtat ctgtagataa aataaaatat taaacctaaa aacaagaccg
1621 cgcctatcaa caaaatgata ggcattaact tgccgctgac gctgtcacta acgttggacg
1681 atttgccgac taaaccttca tcgcccagta accaatctag acgtcaggtg gcacttttcg
1741 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
1801 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
1861 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
1921 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
1981 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
2041 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
2101 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
2161 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
2221 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
2281 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
2341 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
2401 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
2461 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
2521 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
2581 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
2641 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
2701 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
2761 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
2821 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
2881 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
2941 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
3001 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
3061 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
3121 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
3181 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
3241 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
3301 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
3361 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
3421 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
3481 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
3541 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
3601 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
3661 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
3721 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
3781 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
3841 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgaat tcccggg
//
