pIEX-1

价格:1500元

联系方式:I47-825O-882O

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pIEX1载体基本信息

出品公司: Novagen
载体名称: pIEX-1,pIEX1
质粒类型: 昆虫细胞表达载体
高拷贝/低拷贝: 高拷贝
克隆方法: --
启动子: --
载体大小: 3897 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素
筛选标记: --
克隆菌株: --
宿主细胞(系): --
备注: --

pIEX1载体质粒图谱和多克隆位点信息

pIEx-1 载体图谱

pIEX1载体简介

pIEX1载体序列

LOCUS       pIEx-1                3897 bp ds-DNA    circular SYN 25-4-2014
DEFINITION  Vector for high-level expression in insect cells of proteins with a 
            cleavable N-terminal 10xHis-S-Tag cassette plus a C-terminal HSV 
            tag.
ACCESSION   .
VERSION     .
KEYWORDS    pIEx-1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
  JOURNAL   Exported 2017/2/28 from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="synthetic DNA construct"
                     /lab_host="Insect Cells"
                     /mol_type="other DNA"
     enhancer        1..483
                     /gene="Autographa californica hr5"
                     /note="hr5 enhancer"
                     /note="baculovirus early transcription enhancer"
     promoter        487..1078
                     /note="IE1 promoter"
                     /note="promoter of the ie1 gene from the baculovirus 
                     Autographa californica"
     CDS             1084..1086
                     /codon_start=1
                     /product="start codon"
                     /note="ATG"
                     /translation="M"
     CDS             1090..1119
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /note="10xHis"
                     /translation="HHHHHHHHHH"
     CDS             1129..1173
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /note="S-Tag"
                     /translation="KETAAAKFERQHMDS"
     CDS             1189..1206
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /note="thrombin site"
                     /translation="LVPRGS"
     CDS             1225..1239
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /note="enterokinase site"
                     /translation="DDDDK"
     misc_feature    1234..1348
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             1354..1386
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /note="HSV tag"
                     /translation="QPELAPEDPED"
     terminator      1414..1721
                     /note="IE1 terminator"
                     /note="terminator of the ie1 gene from the baculovirus 
                     Autographa californica"
     promoter        1751..1855
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             1856..2716
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2887..3475
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgcgtaaaac acaatcaagt atgagtcata agctgatgtc atgttttgca cacggctcat
       61 aaccgaactg gctttacgag tagaattcta cttgtaacgc acgatcagtg gatgatgtca
      121 tttgtttttc aaatcgagat gatgtcatgt tttgcacacg gctcataaac tcgctttacg
      181 agtagaattc tacgtgtaac gcacgatcga ttgatgagtc atttgttttg caatatgata
      241 tcatacaata tgactcattt gtttttcaaa accgaacttg atttacgggt agaattctac
      301 ttgtaaagca caatcaaaaa gatgatgtca tttgtttttc aaaactgaac tcgctttacg
      361 agtagaattc tacgtgtaaa acacaatcaa gaaatgatgt catttgttat aaaaataaaa
      421 gctgatgtca tgttttgcac atggctcata actaaactcg ctttacgggt agaattctac
      481 gcgcgtcgat gtctttgtga tgcgcgcgac atttttgtag gttattgata aaatgaacgg
      541 atacgttgcc cgacattatc attaaatcct tggcgtagaa tttgtcgggt ccattgtccg
      601 tgtgcgctag catgcccgta acggacctcg tacttttggc ttcaaaggtt ttgcgcacag
      661 acaaaatgtg ccacacttgc agctctgcat gtgtgcgcgt taccacaaat cccaacggcg
      721 cagtgtactt gttgtatgca aataaatctc gataaaggcg cggcgcgcga atgcagctga
      781 tcacgtacgt ccctcgtgtt ccgttcaagg acggtgttat cgacctcaga ttaatgttta
      841 tcggccgact gttttcgtat ccgctcacca aacgcgtttt tgcattaaca ttgtatgtcg
      901 gcggatgttc tatatctaat ttgaataaat aaacgataac cgcgttggtt ttagagggca
      961 taataaaaga aatattgtta tcgtgttcgc cattagggca gtataaattg acgttcatgt
     1021 tggatattgt ttcagttgca agttgacact ggcggcgaca agatcgtgaa caaccaagtg
     1081 accatggcac accaccatca ccaccatcac caccatcact cttctggtaa agaaaccgct
     1141 gctgcgaaat ttgaacgcca gcacatggac tcgccaccgc cttctggtct ggtcccccgg
     1201 ggcagcgcag gttctggtac gattgatgac gacgacaaga gtccgggctt ctcctcaacg
     1261 gatcccaatt gggagctcgt gtacacggcg cgcctgcagg tcgacggtac cggttcgaag
     1321 cttgcggccg cacagctgta tacacgtgca agccagccag aactcgcccc ggaagacccc
     1381 gaggatctcg agcactaagt gattaacctc aggttataca tatattttga atttaattaa
     1441 ttatacatat attttatatt atttttgtct tttattatcg aggggccgtt gttggtgtgg
     1501 ggttttgcat agaaataaca atgggagttg gcgacgttgc tgcgccaaca ccacctccct
     1561 tccctccttt catcatgtat ctgtagataa aataaaatat taaacctaaa aacaagaccg
     1621 cgcctatcaa caaaatgata ggcattaact tgccgctgac gctgtcacta acgttggacg
     1681 atttgccgac taaaccttca tcgcccagta accaatctag acgtcaggtg gcacttttcg
     1741 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     1801 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     1861 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     1921 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     1981 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     2041 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     2101 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     2161 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     2221 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     2281 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     2341 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     2401 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     2461 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     2521 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     2581 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     2641 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     2701 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     2761 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     2821 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     2881 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     2941 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     3001 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     3061 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     3121 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     3181 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     3241 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     3301 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     3361 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     3421 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     3481 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     3541 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     3601 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     3661 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     3721 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     3781 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     3841 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgaat tcccggg
//

      

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