pSuper.Retro.Neo

价格:2500元

联系方式:I47-825O-882O

买家导航

pSR-Neo载体基本信息

出品公司: OligoEngine
载体名称: pSuper.Retro.Neo, pSuper-Retro-Neo, pSuper Retro Neo, pSR-Neo, pSRneo, pSuperRetro-Neo
质粒类型: 哺乳动物细胞逆病毒RNA干扰载体,RNAi
高拷贝/低拷贝: 低拷贝
启动子: CMV
克隆方法: 多克隆位点,限制性内切酶
载体大小: 7641bp
5' 测序引物及序列: pSR-F: GGAAGCCTTGGCTTTTG , binding site: 1241-1257
3' 测序引物及序列: pSR-R: CGAACGTGACGTCATC , binding site: 2645-2629
载体标签: --
载体抗性: 氨苄
筛选标记: 新霉素(Neomycin)
备注: 逆病毒基因沉默载体。
产品目录号: VEC-PRT-0003/0004
稳定性: 稳表达
组成型: 组成型
病毒/非病毒: 逆病毒

pSR-Neo载体质粒图谱和多克隆位点信息

pSuper retro neo载体图谱



RNAi-Ready pSIREN-RetroQ载体特征

pSR-Neo载体简介

pSR-Neo载体序列

LOCUS       pSUPER.retro.neo	7641 bp 	DNA     circular	SYN
DEFINITION  pSUPER.retro.neo
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..7641
                     /organism="pSUPER.retro.neo"
                     /mol_type="other DNA"
     misc_feature    654..1403
                     /label="psi_plus_pack"
     misc_feature    990..1397
                     /label="gag"
     misc_feature    1333..1355
                     /label="MSCV_primer"
     misc_feature    1333..1355
                     /label="pLXSN_5_primer"
     misc_feature    1371..1387
                     /label="pBABE_5_primer"
     misc_feature    1421..1437
                     /label="pBluescriptKS_primer"
     promoter        complement(1560..1578)
                     /label="T7_promoter"
     promoter        complement(1589..2205)
                     /label="CMV_immearly_promoter"
     misc_feature    complement(1652..1672)
                     /label="CMV_fwd_primer"
     misc_feature    complement(1838..2125)
                     /label="CAG_enhancer"
     misc_feature    complement(2490..2509)
                     /label="H1_primer"
     misc_feature    complement(2690..2713)
                     /label="MSCV_rev_primer"
     misc_feature    3091..3110
                     /label="mPGK_F_primer"
     CDS             3243..4037
                     /label="ORF frame 3"
     gene            3246..4034
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
     CDS             complement(3552..4088)
                     /label="ORF frame 2"
     terminator      4101..4149
                     /label="synth_PA_terminator"
     promoter        complement(4735..4764)
                     /label="lac_promoter"
     gene            complement(5847..6707)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(5847..6707)
                     /label="ORF frame 2"
     promoter        complement(6749..6777)
                     /label="AmpR_promoter"
     misc_feature    complement(6936..6958)
                     /label="pGEX_3_primer"
     misc_feature    complement(7157..7288)
                     /label="lacZ_a"
     misc_feature    7271..7293
                     /label="M13_pUC_fwd_primer"
     misc_feature    7421..7440
                     /label="pBRrevBam_primer"
ORIGIN
    1 TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT TGCAAGGCAT
   61 GGAAAATACA TAACTGAGAA TAGAGAAGTT CAGATCAAGG TTAGGAACAG AGAGACAGCA
  121 GAATATGGGC CAAACAGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC AGGGCCAAGA
  181 ACAGATGGTC CCCAGATGCG GTCCCGCCCT CAGCAGTTTC TAGAGAACCA TCAGATGTTT
  241 CCAGGGTGCC CCAAGGACCT GAAATGACCC TGTGCCTTAT TTGAACTAAC CAATCAGTTC
  301 GCTTCTCGCT TCTGTTCGCG CGCTTCTGCT CCCCGAGCTC AATAAAAGAG CCCACAACCC
  361 CTCACTCGGC GCGCCAGTCC TCCGATAGAC TGCGTCGCCC GGGTACCCGT ATTCCCAATA
  421 AAGCCTCTTG CTGTTTGCAT CCGAATCGTG GACTCGCTGA TCCTTGGGAG GGTCTCCTCA
  481 GATTGATTGA CTGCCCACCT CGGGGGTCTT TCATTTGGAG GTTCCACCGA GATTTGGAGA
  541 CCCCTGCCCA GGGACCACCG ACCCCCCCGC CGGGAGGTAA GCTGGCCAGC GGTCGTTTCG
  601 TGTCTGTCTC TGTCTTTGTG CGTGTTTGTG CCGGCATCTA ATGTTTGCGC CTGCGTCTGT
  661 ACTAGTTAGC TAACTAGCTC TGTATCTGGC GGACCCGTGG TGGAACTGAC GAGTTCTGAA
  721 CACCCGGCCG CAACCCTGGG AGACGTCCCA GGGACTTTGG GGGCCGTTTT TGTGGCCCGA
  781 CCTGAGGAAG GGAGTCGATG TGGAATCCGA CCCCGTCAGG ATATGTGGTT CTGGTAGGAG
  841 ACGAGAACCT AAAACAGTTC CCGCCTCCGT CTGAATTTTT GCTTTCGGTT TGGAACCGAA
  901 GCCGCGCGTC TTGTCTGCTG CAGCGCTGCA GCATCGTTCT GTGTTGTCTC TGTCTGACTG
  961 TGTTTCTGTA TTTGTCTGAA AATTAGGGCC AGACTGTTAC CACTCCCTTA AGTTTGACCT
 1021 TAGGTCACTG GAAAGATGTC GAGCGGATCG CTCACAACCA GTCGGTAGAT GTCAAGAAGA
 1081 GACGTTGGGT TACCTTCTGC TCTGCAGAAT GGCCAACCTT TAACGTCGGA TGGCCGCGAG
 1141 ACGGCACCTT TAACCGAGAC CTCATCACCC AGGTTAAGAT CAAGGTCTTT TCACCTGGCC
 1201 CGCATGGACA CCCAGACCAG GTCCCCTACA TCGTGACCTG GGAAGCCTTG GCTTTTGACC
 1261 CCCCTCCCTG GGTCAAGCCC TTTGTACACC CTAAGCCTCC GCCTCCTCTT CCTCCATCCG
 1321 CCCCGTCTCT CCCCCTTGAA CCTCCTCGTT CGACCCCGCC TCGATCCTCC CTTTATCCAG
 1381 CCCTCACTCC TTCTCTAGGC GCCGGAATTA GATCGATCTC TCGAGGTCGA CGGTATCGAT
 1441 AAGCTTGGTA CCGAGCTCGG ATCCACTAGT CCAGTGTGGT GGAATTCTGC AGATATCCAG
 1501 CACAGTGGCG GCCGCTCGAG TCTAGAGGGC CCGTTTAAAC GCTAGCCAGC TTGGGTCTCC
 1561 CTATAGTGAG TCGTATTAAT TTCGATAAGC CAGTAAGCAG TGGGTTCTCT AGTTAGCCAG
 1621 AGAGCTCTGC TTATATAGAC CTCCCACCGT ACACGCCTAC CGCCCATTTG CGTCAATGGG
 1681 GCGGAGTTGT TACGACATTT TGGAAAGTCC CGTTGATTTT GGTGCCAAAA CAAACTCCCA
 1741 TTGACGTCAA TGGGGTGGAG ACTTGGAAAT CCCCGTGAGT CAAACCGCTA TCCACGCCCA
 1801 TTGATGTACT GCCAAAACCG CATCACCATG GTAATAGCGA TGACTAATAC GTAGATGTAC
 1861 TGCCAAGTAG GAAAGTCCCA TAAGGTCATG TACTGGGCAT AATGCCAGGC GGGCCATTTA
 1921 CCGTCATTGA CGTCAATAGG GGGCGTACTT GGCATATGAT ACACTTGATG TACTGCCAAG
 1981 TGGGCAGTTT ACCGTAAATA CTCCACCCAT TGACGTCAAT GGAAAGTCCC TATTGGCGTT
 2041 ACTATGGGAA CATACGTCAT TATTGACGTC AATGGGCGGG GGTCGTTGGG CGGTCAGCCA
 2101 GGCGGGCCAT TTACCGTAAG TTATGTAACG CGGAACTCCA TATATGGGCT ATGAACTAAT
 2161 GACCCCGTAA TTGATTACTA TTAATAACTA GTCAATAATC AATGTCAACG CGTATATCTG
 2221 GCCCGTACAT CGCGAAGCAG CGCAAAACGC CTAACCCTAA GCAGATTCTT CATGCAATTG
 2281 TCGGTCAAGC CTTGCCTTGT TGTAGCTTAA ATTTTGCTCG CGCACTACTC AGCGACCTCC
 2341 AACACACAAG CAGGGAGCAG ATACTGGCTT AACTATGCGG CATCAGAGCA GATTGTACTG
 2401 AGAGTGCACC ATAGGGGATC GGGAGATCTG TGGTCTCATA CAGAACTTAT AAGATTCCCA
 2461 AATCCAAAGA CATTTCACGT TTATGGTGAT TTCCCAGAAC ACATAGCGAC ATGCAAATAT
 2521 TGCAGGGCGC CACTCCCCTG TCCCTCACAG CCATCTTCCT GCCAGGGCGC ACGCGCGCTG
 2581 GGTGTTCCCG CCTAGTGACA CTGGGCCCGC GATTCCTTGG AGCGGGTTGA TGACGTCAGC
 2641 GTTCGAATTC TACCGGGTAG GGGAGGCGCT TTTCCCAAGG CAGTCTGGAG CATGCGCTTT
 2701 AGCAGCCCCG CTGGGCACTT GGCGCTACAC AAGTGGCCTC TGGCCTCGCA CACATTCCAC
 2761 ATCCACCGGC CGGTAGGCGC CAACCGGCTC CGTTCTTTGG TGGCCCCTTC GCGCCACCTT
 2821 CTACTCCTCC CCTAGTCAGG AAGTTCCCCC CCGCCCCGCA GCTCGCGTCG TGCAGGACGT
 2881 GACAAATGGA AGTAGCACGT CTCACTAGTC TCGTGCAGAT GGACAGCACC GCTGAGCAAT
 2941 GGAAGCGGGT AGGCCTTTGG GGCAGCGGCC AATAGCAGCT TTGCTCCTTC GCTTTCTGGG
 3001 CTCAGAGGCT GGGAAGGGGT GGGTCCGGGG GCGGGCTCAG GGGCGGGCTC AGGGGCGGGG
 3061 CGGGCGCCCG AAGGTCCTCC GGAGGCCCGG CATTCTGCAC GCTTCAAAAG CGCACGTCTG
 3121 CCGCGCTGTT CTCCTCTTCC TCATCTCCGG GCCTTTCGAC CTGCAGCCCA AGCTAGCTTA
 3181 CCGGACTCAG ATCAGCTTGA TTCTTCTGAC ACAACAGTCT CGAACTTAAG GCTAGAGCCA
 3241 CCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT
 3301 TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT
 3361 CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC
 3421 TGCAGGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG
 3481 TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC
 3541 AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA
 3601 TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC
 3661 GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG
 3721 AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG
 3781 ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA
 3841 ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC CGCTATCAGG
 3901 ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT
 3961 TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC
 4021 TTGACGAGTT CTTCTGAGCG GGACTCTGGG GTTCGAAATG ACCGACCAAG CGACGCCCAA
 4081 CCTGCCATCA CGATGGCCGC AATAAAATAT CTTTATTTTC ATTACATCTG TGTGTTGGTT
 4141 TTTTGTGTGA ATCGATAGCG ATAAGGATCG GGGATGCATC GATAAAATAA AAGATTTTAT
 4201 TTAGTCTCCA GAAAAAGGGG GGAATGAAAG ACCCCACCTG TAGGTTTGGC AAGCTAGAGA
 4261 ACCATCAGAT GTTTCCAGGG TGCCCCAAGG ACCTGAAATG ACCCTGTGCC TTATTTGAAC
 4321 TAACCAATCA GTTCGCTTCT CGCTTCTGTT CGCGCGCTTC TGCTCCCCGA GCTCAATAAA
 4381 AGAGCCCACA ACCCCTCACT CGGCGCGCCA GTCCTCCGAT AGACTGCGTC GCCCGGGTAC
 4441 CCGTGTATCC AATAAACCCT CTTGCAGTTG CATCCGACTT GTGGTCTCGC TGTTCCTTGG
 4501 GAGGGTCTCC TCTGAGTGAT TGACTACCCG TCAGCGGGGG TCTTTCATGG GTAACAGTTT
 4561 CTTGAAGTTG GAGAACAACA TTCTGAGGGT AGGAGTCGAA TATTAAGTAA TCCTGACTCA
 4621 ATTAGCCACT GTTTTGAATC CACATACTCC AATACTCCTG AAATAGTTCA TTATGGACAG
 4681 CGCAGAAAGA GCTGGGGAGA ATTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA
 4741 CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA
 4801 ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA
 4861 TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG
 4921 CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG
 4981 GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA
 5041 GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC
 5101 CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA
 5161 GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG
 5221 ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT
 5281 CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT
 5341 GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG
 5401 TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC
 5461 AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC
 5521 ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA
 5581 GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC
 5641 AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG
 5701 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA
 5761 AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT
 5821 ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA
 5881 GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG
 5941 ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA
 6001 CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT
 6061 CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT
 6121 AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA
 6181 CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA
 6241 TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA
 6301 AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT
 6361 GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA
 6421 GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG
 6481 CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC
 6541 TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA
 6601 TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT
 6661 GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT
 6721 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT
 6781 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC
 6841 GTCTAAGAAA CCATTATTAT CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC
 6901 TTTCGTCTCG CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG
 6961 ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA
 7021 GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT AACTATGCGG CATCAGAGCA GATTGTACTG
 7081 AGAGTGCACC ATATGCGGTG TGAAATACCG CACAGATGCG TAAGGAGAAA ATACCGCATC
 7141 AGGCGCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG GGCGATCGGT GCGGGCCTCT
 7201 TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA GGCGATTAAG TTGGGTAACG
 7261 CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCGC AAGGAATGGT GCATGCAAGG
 7321 AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG
 7381 CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG
 7441 CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGC
 7501 GATTAGTCCA ATTTGTTAAA GACAGGATAT CAGTGGTCCA GGCTCTAGTT TTGACTCAAC
 7561 AATATCACCA GCTGAAGCCT ATAGAGTACG AGCCATAGAT AAAATAAAAG ATTTTATTTA
 7621 GTCTCCAGAA AAAGGGGGGA A
//

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pSR-Neo载体应用举例

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