pDEST8

2000 2300 2600 3000 询价

价格：元

联系方式：I47-825O-882O

买家导航

pDEST8载体质粒基本信息

出品公司:	Invitrigen
载体名称:	pDEST8
质粒类型:	Gateway载体；昆虫细胞表达载体
高拷贝/低拷贝:	高拷贝
启动子:	Polyhedrin
克隆方法:	Gateway
载体大小:	6526bp
5' 测序引物及序列:	5'd[AAATGATAACCATCTCGC]3'
3' 测序引物及序列:	--
载体标签:	无标签
载体抗性:	氨苄青霉素
筛选标记:	Gentamycin庆大霉素
克隆菌株:	ccdB阳性筛选 DB3.1 感受态细胞，阴性筛选用 OmniMAX2-T1 感受态细胞
备注:	--
产品目录号:	11804-010
稳定性:	稳表达
组成型/诱导型:	组成型
病毒/非病毒:	非病毒

pDEST8质粒图谱载体图谱和pDEST8载体序列质粒序列多克隆位点信息

pDEST8载体图谱

pDEST8质粒载体简介

Gateway昆虫细胞表达载体包括：

pDEST8 不带标签
pDEST10 His标签 N末端
pDEST20 GST标签 N末端

pDEST8质粒序列载体序列

CGTATACTCCGGAATATTAATAGATCATGGAGATAATTAAAATGATAACCATCTCGCAAATAAATAAGTA
TTTTACTGTTTTCGTAACAGTTTTGTAATAAAAAAACCTATAAATATTCCGGATTATTCATACCGTCCCA
CCATCGGGCGCGGATCATCACAAGTTTGTACAAAAAAGCTGAACGAGAAACGTAAAATGATATAAATATC
AATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAACATATCCAGTCAC
TATGGCGGCCGCTAAGTTGGCAGCATCACCCGACGCACTTTGCGCCGAATAAATACCTGTGACGGAAGAT
CACTTCGCAGAATAAATAAATCCTGGTGTCCCTGTTGATACCGGGAAGCCCTGGGCCAACTTTTGGCGAA
AATGAGACGTTGATCGGCACGTAAGAGGTTCCAACTTTCACCATAATGAAATAAGATCACTACCGGGCGT
ATTTTTTGAGTTATCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACC
ACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCT
ATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTA
TCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGAC
GGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTT
CATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTG
TTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCC
TGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCA
TGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTCTG
TGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCG
TAAACGCGTGGATCCGGCTTACTAAAAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTA
TAAGAATATATACTGATATGTATACCCGAAGTATGTCAAAAAGAGGTGTGCTATGAAGCAGCGTATTACA
GTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGGTAAG
CACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATG
GCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGAAATGCAG
TTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTG
ACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGA
ACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCG
GTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACC
TGATGTTCTGGGGAATATAAATGTCAGGCTCCCTTATACACAGCCAGTCTGCAGGTCGACCATAGTGACT
GGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATTGATATT
TATATCATTTTACGTTTCTCGTTCAGCTTTCTTGTACAAAGTGGTGATAGCTTGTCGAGAAGTACTAGAG
GATCATAATCAGCCATACCACATTTGTAGAGGTTTTACTTGCTTTAAAAAACCTCCCACACCTCCCCCTG
AACCTGAAACATAAAATGAATGCAATTGTTGTTGTTAACTTGTTTATTGCAGCTTATAATGGTTACAAAT
AAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAA
ACTCATCAATGTATCTTATCATGTCTGGATCTGATCACTGCTTGAGCCTAGGAGATCCGAACCAGATAAG
TGAAATCTAGTTCCAAACTATTTTGTCATTTTTAATTTTCGTATTAGCTTACGACGCTACACCCAGTTCC
CATCTATTTTGTCACTCTTCCCTAAATAATCCTTAAAAACTCCATTTCCACCCCTCCCAGTTCCCAACTA
TTTTGTCCGCCCACAGCGGGGCATTTTTCTTCCTGTTATGTTTTTAATCAAACATCCTGCCAACTCCATG
TGACAAACCGTCATCTTCGGCTACTTTTTCTCTGTCACAGAATGAAAATTTTTCTGTCATCTCTTCGTTA
TTAATGTTTGTAATTGACTGAATATCAACGCTTATTTGCAGCCTGAATGGCGAATGGACGCGCCCTGTAG
CGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCG
CCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATC
GGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGA
TGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTT
AATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAG
GGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAA
CAAAATATTAACGTTTACAATTTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTA
TTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATT
GAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCT
TCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTG
GGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAA
TGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACT
CGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACG
GATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTAC
TTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCG
CCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTA
GCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAA
TAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTAT
TGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAG
CCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCG
CTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGAT
TGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAA
ATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAG
ATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTT
GCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACT
GTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTC
TGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACG
ATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGA
ACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAA
AGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAA
CGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCG
TCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGC
CTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTG
AGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGC
CTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCAGACCAGCCGCGTAACCTGGCA
AAATCGGTTACGGTTGAGTAATAAATGGATGCCCTGCGTAAGCGGGTGTGGGCGGACAATAAAGTCTTAA
ACTGAACAAAATAGATCTAAACTATGACAATAAAGTCTTAAACTAGACAGAATAGTTGTAAACTGAAATC
AGTCCAGTTATGCTGTGAAAAAGCATACTGGACTTTTGTTATGGCTAAAGCAAACTCTTCATTTTCTGAA
GTGCAAATTGCCCGTCGTATTAAAGAGGGGCGTGGCCAAGGGCATGGTAAAGACTATATTCGCGGCGTTG
TGACAATTTACCGAACAACTCCGCGGCCGGGAAGCCGATCTCGGCTTGAACGAATTGTTAGGTGGCGGTA
CTTGGGTCGATATCAAAGTGCATCACTTCTTCCCGTATGCCCAACTTTGTATAGAGAGCCACTGCGGGAT
CGTCACCGTAATCTGCTTGCACGTAGATCACATAAGCACCAAGCGCGTTGGCCTCATGCTTGAGGAGATT
GATGAGCGCGGTGGCAATGCCCTGCCTCCGGTGCTCGCCGGAGACTGCGAGATCATAGATATAGATCTCA
CTACGCGGCTGCTCAAACCTGGGCAGAACGTAAGCCGCGAGAGCGCCAACAACCGCTTCTTGGTCGAAGG
CAGCAAGCGCGATGAATGTCTTACTACGGAGCAAGTTCCCGAGGTAATCGGAGTCCGGCTGATGTTGGGA
GTAGGTGGCTACGTCTCCGAACTCACGACCGAAAAGATCAAGAGCAGCCCGCATGGATTTGACTTGGTCA
GGGCCGAGCCTACATGTGCGAATGATGCCCATACTTGAGCCACCTAACTTTGTTTTAGGGCGACTGCCCT
GCTGCGTAACATCGTTGCTGCTGCGTAACATCGTTGCTGCTCCATAACATCAAACATCGACCCACGGCGT
AACGCGCTTGCTGCTTGGATGCCCGAGGCATAGACTGTACAAAAAAACAGTCATAACAAGCCATGAAAAC
CGCCACTGCGCCGTTACCACCGCTGCGTTCGGTCAAGGTTCTGGACCAGTTGCGTGAGCGCATACGCTAC
TTGCATTACAGTTTACGAACCGAACAGGCTTATGTCAACTGGGTTCGTGCCTTCATCCGTTTCCACGGTG
TGCGTCACCCGGCAACCTTGGGCAGCAGCGAAGTCGAGGCATTTCTGTCCTGGCTGGCGAACGAGCGCAA
GGTTTCGGTCTCCACGCATCGTCAGGCATTGGCGGCCTTGCTGTTCTTCTACGGCAAGGTGCTGTGCACG
GATCTGCCCTGGCTTCAGGAGATCGGAAGACCTCGGCCGTCGCGGCGCTTGCCGGTGGTGCTGACCCCGG
ATGAAGTGGTTCGCATCCTCGGTTTTCTGGAAGGCGAGCATCGTTTGTTCGCCCAGGACTCTAGCTATAG
TTCTAGTGGTTGGCTA

pAAVLINK-EF1a-HA-5'-ITSN1	pBBR1MCS2-Tac-mCherry
pENTR5'/CMVp	pCEP4-Flag-N
pAAVLINK-EF1a-HA-5'-DIP2C	pcDNA3.1-mRFP
pET28a-mOrange(M163A)	pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CADPS-FLAG
pLVX-AmCyan1-N1	pcDNA4/TO/Myc-His/LacZ
pQE-16	pcDNA3.1/Zeo(-)
pBad33	pLenti6/V5-GW/lacZ
pDB262	pHBT-GFP-NOS
pGFP22	pBluescript II KS(-)
pGEX-6p-1-AT5G48650	pIEX-1
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PHIP-FLAG	pGL4.21[luc2P/Puro]
YEp16	pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM5A-FLAG
pDEST8	pQLinkN
pMAL-p5x	FUW-tetO-hOCT4
pBR327	pCEP4-DsRed2
pAAVLINKv1-pCALM1-Cre-3'-RAI1-FLAG	pAAVLINK-EF1a-5'-PXDN
pDNAPolbeta_TA	pCRII-TOPO/22-1
pcDNA6.2/nTC-Tag-DEST	pFN10A (ACT)
pCDEF-HA-Ub-K63R	YEp620
pIEX-3	pIEx/Bac-3
pET-53-DEST	pBTB-5
pAAVLINKv1-pCALM1-Cre-3'-ITSN1-FLAG	pS-hydro_TA
pBARMTE1	pAAV-hSyn-Cepheid1s-WPRE
pAP1(PMA)-TA-Luc	pENTR-L1pLaclacZaL4
pcDNA3.1+N-DYK-P2A	pHOOK1
ppyrG	YEp24
pSwitch	pHisB-Sumo-NeMeHg
pGEX-6p-1-hsG3BP2b	pVK101
pSuper.Retro.Neo	pCRE-DD-AmCyan1
pMMB66EH	Pgex-HRV3C
pFastBac1-His-C	pNov.aro-bgl1-640
pCold-SUMO	pIZT/V5-His
pSP65-F1+	pHT01
pMCS-Green-Renilla-Luc	pRI101-AN
pBABE-HAII	pAMβ1
pSW510	pHR31
pEYFP-Mem	pAAV-CAG-NES-YTnC
pWY113 pET28a_AtmtE2	p19D+
pAX01-ComK	pDM4
pGL4.18[luc2P/Neo]	pPM408
pAAV-EFS-DIO-GRAB_OT1.0	pYES2-EGFP
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-POGZ-FLAG	pYC25
pIRESpuro3	p1JI008
pUTK21	pHIL-D2
pLC4-14	pSEAP2-Basic
pL-brevisGH1-TA	pShuttle-CMV
pAAVLINK-EF1a-HA-5-RALGAPB	pENTR U6
pRetroX-Tight-Hyg	pcDNA3.1-CNTRFv1-HA-His
pAAV-TRE-CAG-Fluc	pFR-Luc
pHY300PLK	pUC19

pEG302 22aa SunTag NtDRMcd (no NLS) g4+g	pBIG1a
pMIH-TOMM20-Halo	LeGO-iG
p6XHis-T7(P266L)	Fuw-dCas9-Dnmt3a_IM
pcDNA3 Connexin43-GFP-APEX2	pXR002:EF1a-dCasRx-2A-EGFP
murine TCR OTI-2A.pMIG II	pCITEp65
pGHYB	pcDNA5-T/O-Ds1-mCherry
SIRT6 Flag	pHCMV_DUGV_GP
pMR-A-3	pCDNA3 Flag p38 alpha
pLTR-RD114A	pcDNA3.1(+) CircRNA Mini Vector
pRK5-HA-SH3BP4 W92A	pLIC-SM-HA
pET16B.Pfu	pNG9
pCutamp	GW1-Peredox-mCherry-NLS
BCR/ABL P190 transgenic construct	pJRH-1179 U6-reci Gag-Cas9 v2
pEGFP-C1 F-tractin-EGFP	pCI-6.20
pGP-CMV-Gcamp6m	pCS2+HyPer7-NLS
pCMT-flp	pCMV-HA-JMJD3
pKAR2-Br512	pAKTaq
pShuttle-CMV-GFP U6promoter	pINDUCER21-puro_Gateway-3xFLAG
pLenti-MP2	CAPTURE2_pLVX-EF1a-dCas9-CBio-IRES-zsGre
PX551	phage UbiC G3BP1-SNAP
mAzurite-8xbiRhoBAST-24xMS2v6	MG1655 ΔendA ΔrecA (DE3)
EGFP-LC3	pEBTet_NAD-Snifit-mito
pBSK	pEF1a-Usp9x_CD-mCherry
shYAP1	pBK092
mARG1 cassette 3	BB3cK_pGAP_23*_pTEF_Cas9
GFP-MAP1B	pscAAV-CAG-GFP
PA-GFPmt	pAc-sgRNA-Cas9
pTA-HUH	GAG-CRErec
pT7-SpCas9_sgRNA-site1 (RTW443)	pGL3 3'UTR reporter WT 1.3 kb CD274 Hs 3
pKO (Hyg)	pQUAST>stop>mCD8-GFP
pDawn-sfGFP	pLminP_Luc2P_RE4
CAMK2D gRNA (BRDN0001146112)	pBLO 64.1
Myc tag WT PTB	pTM1_VP30_ZEBOV
pcDNA3.1-TYMS	PB533_15F11-sfGFP
pQCXIP-GFP-LacR	pSI-Target-AID-NG
AdEasier-1 cells (strain)	pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA
CD109-bio-His	pCI-neo.mCherry-NEDD4
pHIV-Luciferase	pX330_SpCas9_USP7 Exon 3 gRNA
MP6	pGEX6p-1-BCCP-PupE
pQCasTns(Vch)-entry(BsaI)	pDriver
pCRISPR-cBEST	pMXs-hSOX2
pCDH-DCX-TurboID-V5-puro	pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W
Double UP mNeon to mScarlet	pT7-hMLH1dn for IVT
pCAG-FAST-p10	pAAV2/9n
pcDNA3.1 SIRT6_H133Y	pAAVS1-PC-GCaMP6f
pPB-AMBRA1-3xFLAG	LentiGuide_DKO
pRK5-HA-Ubiquitin-K29R	pcDNA3.1_AktPH-mCherry
AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-	pcDNA3.1-ERBB4
pRH2502	pET28GST-LIC
pBiFC-VN155(I152L)	pCMV-mA1-BE3

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pDEST8

买家导航

pDEST8载体质粒基本信息

pDEST8质粒图谱载体图谱和pDEST8载体序列质粒序列多克隆位点信息

pDEST8质粒载体简介

pDEST8质粒序列载体序列

pDEST8载体图谱质粒图谱pdf版和pDEST8载体序列质粒序列等相关资料下载

pDEST8质粒载体应用举例

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