| 质粒名称: | pLTR-RD114A |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 17576 |
| 存储实验室: | Jakob Reiser |
|
载体骨架基本信息 |
|
| 载体名称: | pEGFP-C1 (Clontech) |
| 载体出品公司: | -- |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 3417 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | RD114 envelope glycoprotein,hybrid RD114 Env with cytoplasmic tail domain derived from the MLV 4070A Env |
| 物种来源: | RD114 retrovirus |
| 插入大小: | 2146 bp |
| 基因突变: | -- |
| GenBank编号 | NC_009889 |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pLTR-RD114A was a gift from Jakob Reiser (Addgene plasmid # 17576 ; http://n2t.net/addgene:17576 ; RRID:Addgene_17576) |
| 参考文献部分: | Transduction of bone-marrow-derived mesenchymal stem cells by using lentivirus vectors pseudotyped with modified RD114 envelope glycoproteins. Zhang XY, La Russa VF, Reiser J. J Virol. 2004 Feb . 78(3):1219-29. 10.1128/JVI.78.3.1219-1229.2004 PubMed 14722277 |
买家导航
pLTRRD114A载体基本信息
pLTRRD114A载体图谱质粒图谱和多克隆位点信息
pLTRRD114A载体简介
pLTRRD114A载体序列
LOCUS pLTR-RD114A 6258 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pLTR-RD114A
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6258)
AUTHORS Zhang XY, La Russa VF, Reiser J
TITLE Transduction of bone-marrow-derived mesenchymal stem cells by using
lentivirus vectors pseudotyped with modified RD114 envelope
glycoproteins.
JOURNAL J Virol. 2004 Feb . 78(3):1219-29.
PUBMED 14722277
REFERENCE 2 (bases 1 to 6258)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6258
/organism="synthetic DNA construct"
/mol_type="other DNA"
polyA_signal 3017..3138
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3054..3073)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 3108..3127
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(3145..3600)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3282..3303)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 3494..3513
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 3627..3731
/gene="bla"
/label=AmpR promoter
promoter 3733..4090
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(3756..3776)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 3941..4076
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 4003..4022
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 4125..4919
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(4179..4198)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 4789..4808
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(5107..5126)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 5151..5198
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 5527..6115
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6016..6035
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaacaatgc tgcttgtgcc
61 tggctagaag cacaagagga ggaagaggtg ggttttccag tcacacctca ggtaccttta
121 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
181 ctggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
241 acacaaggct acttccctga ttggcagaac tacacaccag ggccaggggt cagatatcca
301 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
361 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatggaat ggatgaccct
421 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
481 gagctgcatc cggagtactt caagaactgc tgacatcgag cttgctacaa gggactttcc
541 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
601 tgctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
661 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagctgatc
721 ccccgggctg caggaattta tgaaatcctt tatgggggac cccccccttt gtcaaccttg
781 ctcaattcct tctccccctc cgatcctaag actgatttac aagcccgact aaaagggctg
841 caagcggtgc aggcccaaat ctggacaccc ctggccgaat tgtaccggcc aggacatcca
901 caaactagcc acccatttca ggtgggagac tccgtgtacg tccggcggca ccgctctcaa
961 ggattggagc ctcgttggaa gggaccttac atcgtcctgc tgaccacgcc caccgccata
1021 aaggttgacg ggatcgccgc ctggattcac gcatcgcacg ccaaggcagc cccaaaaacc
1081 cctggaccag aaactcccaa aacctggaag ctccgccgtt cggagaaccc tcttaagata
1141 agactctccc gtgtctgact gctaatccac cttgtccctg tactaaccca aaatgaaact
1201 cccaacagga atggtcattt tatgtagcct aataatagtt cgggcagggt ttgacgaccc
1261 ccgcaaggct atcgcattag tacaaaaaca acatggtaaa ccatgcgaat gcagcggagg
1321 gcaggtatcc gaggccccac cgaactccat ccaacaggta acttgcccag gcaagacggc
1381 ctacttaatg accaaccaaa aatggaaatg cagagtcact ccaaaaaatc tcacccctag
1441 cgggggagaa ctccagaact gcccctgtaa cactttccag gactcgatgc acagttcttg
1501 ttatactgaa taccggcaat gcagggcgaa taataagaca tactacacgg ccaccttgct
1561 taaaatacgg tctgggagcc tcaacgaggt acagatatta caaaacccca atcagctcct
1621 acagtcccct tgtaggggct ctataaatca gcccgtttgc tggagtgcca cagcccccat
1681 ccatatctcc gatggtggag gacccctcga tactaagaga gtgtggacag tccaaaaaag
1741 gctagaacaa attcataagg ctatgcatcc tgaacttcaa taccacccct tagccctgcc
1801 caaagtcaga gatgacctta gccttgatgc acggactttt gatatcctga ataccacttt
1861 taggttactc cagatgtcca attttagcct tgcccaagat tgttggctct gtttaaaact
1921 aggtacccct acccctcttg cgatacccac tccctcttta acctactccc tagcagactc
1981 cctagcgaat gcctcctgtc agattatacc tcccctcttg gttcaaccga tgcagttctc
2041 caactcgtcc tgtttatctt cccctttcat taacgatacg gaacaaatag acttaggtgc
2101 agtcaccttt actaactgca cctctgtagc caatgtcagt agtcctttat gtgccctaaa
2161 cgggtcagtc ttcctctgtg gaaataacat ggcatacacc tatttacccc aaaactggac
2221 aggactttgc gtccaagcct ccctcctccc cgacattgac atcatcccgg gggatgagcc
2281 agtccccatt cctgccattg atcattatat acatagacct aaacgagctg tacagttcat
2341 ccctttacta gctggactgg gaatcaccgc agcattcacc accggagcta caggcctagg
2401 tgtctccgtc acccagtata caaaattatc ccatcagtta atatctgatg tccaagtctt
2461 atccggtacc atacaagatt tacaagacca ggtagactcg ttagctgaag tagttctcca
2521 aaataggagg ggactggacc tactaacggc agaacaagga ggaatttgtt tagccttaca
2581 agaaaaatgc tgtttttatg ctaacaagtc aggaattgtg agaaacaaaa taagaaccct
2641 acaagaagaa ttacaaaaac gcagggaaag cctggcatcc aaccctctct ggaccgggct
2701 gcagggcttt cttccgtacc tcctacctct cctgggaccc ctactcaccc tcctactcat
2761 actaaccatt gggccatgcg ttttcaatcg attggtccaa tttgttaaag acaggatctc
2821 agtggtccag gctctggttt tgactcagca atatcaccag ctaaaaccca tagagtacga
2881 gccatgaaga tccaccggat ctagataact gatcataatc agccatacca catttgtaga
2941 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
3001 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
3061 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
3121 actcatcaat gtatcttaac gcgtaaattg taagcgttaa tattttgtta aaattcgcgt
3181 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
3241 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
3301 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
3361 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
3421 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
3481 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
3541 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
3601 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
3661 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
3721 aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg
3781 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
3841 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
3901 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
3961 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
4021 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
4081 cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
4141 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
4201 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
4261 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc
4321 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
4381 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
4441 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
4501 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
4561 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
4621 cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg
4681 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
4741 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
4801 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
4861 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
4921 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
4981 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
5041 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag
5101 gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa
5161 aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca
5221 gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc
5281 ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt
5341 cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa
5401 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
5461 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
5521 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
5581 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
5641 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
5701 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
5761 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
5821 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
5881 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
5941 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
6001 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
6061 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
6121 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
6181 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gccatgcatt agttattacc
6241 ggactcagat ctcgagac
//
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