| 质粒名称: | pTA-HUH |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 221152 |
| 存储实验室: | Sheng Yang |
|
载体骨架基本信息 |
|
| 载体名称: | pMD18T |
| 载体出品公司: | Clontech |
| 完整质粒大小: | 6871bp |
| 载体类型: | 细菌表达、, Yeast Expression |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | HisG-URA3-HisG |
| 物种来源: | Yarrowia lipolytica, Salmonella |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | HindIII (unknown if destroyed) |
| 3’克隆位点: | EcoRI (unknown if destroyed) |
|
文献引用方法 |
|
| 材料和方法部分: | pTA-HUH was a gift from Sheng Yang (Addgene plasmid # 221152 ; http://n2t.net/addgene:221152 ; RRID:Addgene_221152) |
| 参考文献部分: | Multiplex gene editing of the Yarrowia lipolytica genome using the CRISPR-Cas9 system. Gao S, Tong Y, Wen Z, Zhu L, Ge M, Chen D, Jiang Y, Yang S. J Ind Microbiol Biotechnol. 2016 Aug;43(8):1085-93. doi: 10.1007/s10295-016-1789-8. Epub 2016 Jun 27. 10.1007/s10295-016-1789-8 [pii] PubMed 27349768 |
买家导航
pTA-HUH载体质粒基本信息
pTA-HUH质粒图谱载体图谱和pTA-HUH载体序列质粒序列多克隆位点信息
pTA-HUH质粒载体简介
pTA-HUH质粒序列载体序列
LOCUS pTA-HUH 6871 bp DNA circular SYN 15-APR-2025
DEFINITION HisG-URA3-HisG recycling type marker in pTAs for Yarrowia
lipolytica.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6871)
AUTHORS Gao S, Tong Y, Wen Z, Zhu L, Ge M, Chen D, Jiang Y, Yang S
TITLE Multiplex gene editing of the Yarrowia lipolytica genome using the
CRISPR-Cas9 system.
JOURNAL J Ind Microbiol Biotechnol. 2016 Aug;43(8):1085-93. doi:
10.1007/s10295-016-1789-8. Epub 2016 Jun 27.
PUBMED 27349768
REFERENCE 2 (bases 1 to 6871)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 15, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1007/s10295-016-1789-8"; journalName: "J Ind Microbiol
Biotechnol"; date: "2016-08"; volume: "43"; issue: "8"; pages:
"1085-93"
COMMENT Sequence Label: pTA-HUH
FEATURES Location/Qualifiers
source 1..6871
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind complement(29..51)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 151..170
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 364..386
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 378..395
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 379..395
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4650..4666)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4650..4666)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4663..4685)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4674..4690
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(join(4698..4704,4705..4722,4723..4728))
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4743..4764
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(4881..4898)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5052..5640)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5132..5151)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(5811..6602,6603..6671))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6434..6453
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6672..6776)
/gene="bla"
/label=AmpR promoter
primer_bind 6844..6862
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttggggct gttccagtca
421 atcagggtat tgaagctcat ggtctttact ccatcacagg gttccgcctt atccggccta
481 cagaacccaa aatatcaacg cattacgtag gcctgataag cgcagcgcca tcaggcgtca
541 gatcactcca tcatcttctc gatcggcagt accagaatcg agctggcgcc aagcgctttc
601 agtttctcca tggtttccca gaacaacgtt tcgctgctga ccatgtgcat cgccacgcgc
661 tgttgctcgc ctgccagcgg cagaattgtc ggcctttcgg cgcctggcag cagggcgata
721 acctcttcca ggcgttcact tggcgcgtgc atcatgatgt atttcgattc gcgcgcctga
781 atcacgccct gaatacgggt cagcaattta tcgatcagct cttgcttgct ctgtgccatc
841 tcaccgtcgc gctgaatcag acaggcttta gagcggtaga taacttcgac ttcacgcagg
901 ccgttagctt caagcgtcgc gccggtagag accaaatcgc agatagcgtc ggccagcccc
961 gcgcgcggcg cgacttcgac agaaccattt aacagacacg atttaaaaga gacgcctttc
1021 tggtcgaggt agcgtttgag gaggtgcgga tatgaggtag cgatacgttt accgtccagc
1081 gcggccgggc cgtcccaggc ttcgtcaacc ggtgttgcca gcgataaacg gcagccgccg
1141 aagtcaagac ggcgcagggt taaatagcgt ggatcttcgc cctgtgcgcg gcggttgagt
1201 agctcttctt ccagcacgtt ttcgccgata ataccgagat cgaccacgcc atccattacc
1261 agacccggaa tgtcatcatc acgcacgcgc aggatatcaa tcggcatgtt ttccgccatc
1321 gcaatcaggc gctgagtgtg taaattaatt tttatgccgc agcgggccag caattctcgt
1381 gaatcatcgc ttaaacggcc tgatttctga atagctatgc gtaagcgggt gttgtctaac
1441 attctgcgtt cctctttatc ctgtctgaac cggtctgtat cgcgcgccaa aaaaaaagcc
1501 cccggaagat gatcttccgg gggctttctc atgcgttcat gcaccactgg aagatccggg
1561 atcccgggtg tagtggtagt gcagtggtgg tattgtgact ggggatgtag ttgagaataa
1621 gtcatacaca agtcagcttt cttcaagcct catataagta taagtagttc aatcgtatta
1681 gctacttgta cccagcatct ccgtatcgag aaacacaaca acatgcccca ttggacagat
1741 catgcggata cacaggttgt gcagtatcat acatactcga tcagacaggt cgtctgacca
1801 tcatacaagc tgaacaagcg ctccatactt gcacgctctc tatatacaca gttaaattac
1861 atatccatag tctaacctct aacagttaat cttctggtaa gcctcccagc cagccttctg
1921 gtatcgcttg gcctcctcaa taggatctcg gttctggccg tacagacctc ggccgacaat
1981 tatgatatcc gttccggtag acatgacatc ctcaacagtt cggtactgct gtccgagagc
2041 gtctcccttg tcgtcaagac ccaccccggg ggtcagaata agccagtcct cagagtcgcc
2101 cttaggtcgg ttctgggcaa tgaagccaac cacaaactcg gggtcggatc gggcaagctc
2161 aatggtctgc ttggagtact cgccagtggc cagagagccc ttgcaagaca gctcggccag
2221 catgagcaga cctctggcca gcttctcgtt gggagagggg accaggaact ccttgtactg
2281 ggagttctcg tagtcagaga cgtcctcctt cttctgttca gagacagttt cctcggcacc
2341 agctcgcagg ccagcaatga ttccggttcc gggtacaccg tgggcgttgg tgatatcgga
2401 ccactcggcg attcggtaga caccgttctt gtactggtgc ttgacagtgt tgccaatatc
2461 tgcgaacttt ctgtcctcga acaggaagaa accgtgctta agagcaagtt ccttgagggg
2521 gagcacagtg ccggcgtagg tgaagtcgtc aatgatgtcg atatgggtct tgatcatgca
2581 cacataaggt ccgaccttat cggcaagctc aatgagctcc ttggtggtgg taacatccag
2641 agaagcacac aggttggttt tcttggctgc cacgagcttg agcactcgag cggcaaaggc
2701 ggacttgtgg acgttagctc gagcttcgta ggagggcatt ttggtggtga agaggagact
2761 gaaataaatt tagtctgcag aactttttat cggaacctta tctggggcag tgaagtatat
2821 gttatggtaa tagttacgag ttagttgaac ttatagatag actggactat acggctatcg
2881 gtccaaatta gaaagaacgt caatggctct ctgggcgtcg cctttgccga caaaaatgtg
2941 atcatgatga aagccagcaa tgacgttgca gctgatattg ttgtcggcca accgcgccga
3001 aaacgcagct gtcagaccca cagcctccaa cgaagaatgt atcgtcaaag tgatccaagc
3061 acactcatag ttggagtcgt actccaaagg cggcaatgac gagtcagaca gatactcgtc
3121 gaccttttcc ttgggaacca ccaccgtcag cccttctgac tcacgtattg tagccaccga
3181 cacaggcaac agtccgtgga tagcagaata tgtcttgtcg gtccatttct caccaacttt
3241 aggcgtcaag tgaatgttgc agaagaagta tgtgccttca ttgagaatcg gtgttgctga
3301 tttcaataaa gtcttgagat cagtttggcc agtcatgttg tggggggtaa ttggattgag
3361 ttatcgccta cagtctgtac aggtatactc gctgcccact ttatactttt tgattccgct
3421 gcacttgaag caatgtcgtt taccaaaagt gagaatgctc cacagaacac accggggtac
3481 cccgctgttc cagtcaatca gggtattgaa gctcatggtc tttactccat cacagggttc
3541 cgccttatcc ggcctacaga acccaaaata tcaacgcatt acgtaggcct gataagcgca
3601 gcgccatcag gcgtcagatc actccatcat cttctcgatc ggcagtacca gaatcgagct
3661 ggcgccaagc gctttcagtt tctccatggt ttcccagaac aacgtttcgc tgctgaccat
3721 gtgcatcgcc acgcgctgtt gctcgcctgc cagcggcaga attgtcggcc tttcggcgcc
3781 tggcagcagg gcgataacct cttccaggcg ttcacttggc gcgtgcatca tgatgtattt
3841 cgattcgcgc gcctgaatca cgccctgaat acgggtcagc aatttatcga tcagctcttg
3901 cttgctctgt gccatctcac cgtcgcgctg aatcagacag gctttagagc ggtagataac
3961 ttcgacttca cgcaggccgt tagcttcaag cgtcgcgccg gtagagacca aatcgcagat
4021 agcgtcggcc agccccgcgc gcggcgcgac ttcgacagaa ccatttaaca gacacgattt
4081 aaaagagacg cctttctggt cgaggtagcg tttgaggagg tgcggatatg aggtagcgat
4141 acgtttaccg tccagcgcgg ccgggccgtc ccaggcttcg tcaaccggtg ttgccagcga
4201 taaacggcag ccgccgaagt caagacggcg cagggttaaa tagcgtggat cttcgccctg
4261 tgcgcggcgg ttgagtagct cttcttccag cacgttttcg ccgataatac cgagatcgac
4321 cacgccatcc attaccagac ccggaatgtc atcatcacgc acgcgcagga tatcaatcgg
4381 catgttttcc gccatcgcaa tcaggcgctg agtgtgtaaa ttaattttta tgccgcagcg
4441 ggccagcaat tctcgtgaat catcgcttaa acggcctgat ttctgaatag ctatgcgtaa
4501 gcgggtgttg tctaacattc tgcgttcctc tttatcctgt ctgaaccggt ctgtatcgcg
4561 cgccaaaaaa aaagcccccg gaagatgatc ttccgggggc tttctcatgc gttcatgcac
4621 cactggaaga tccggaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
4681 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
4741 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
4801 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
4861 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
4921 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
4981 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
5041 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
5101 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
5161 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
5221 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
5281 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
5341 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
5401 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
5461 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
5521 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
5581 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
5641 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
5701 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
5761 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
5821 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
5881 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
5941 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
6001 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
6061 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
6121 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
6181 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
6241 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
6301 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
6361 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
6421 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
6481 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
6541 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
6601 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
6661 tgaatactca tactcttcct ttttcaatct tattgaagca tttatcaggg ttattgtctc
6721 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
6781 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
6841 aaaaataggc gtatcacgag gccctttcgt c
//
