pYC25

价格:1200元

联系方式:I47-825O-882O

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载体基本信息

出品人: Irene Castano Navarro(2015)
载体名称: pYC25
质粒类型: 酿酒酵母载体;复制型载体;过表达载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: TEF1
载体大小: 6418 bp
5' 测序引物及序列: M13/pUC fwd primer 5’ CCAGTCACGACGTTGTAAAACG 3’
3' 测序引物及序列: GFP-R primer 5’ ACCATCTAATTCAACAAGAATTGGGACAAC 3’
载体标签: 黄色荧光蛋白YFP tag
载体抗性: 氨苄青霉素
筛选标记: URA3
克隆菌株: DH10B感受态细胞 ,30°C
宿主细胞(系): 出芽酵母(酿酒酵母)、白色念珠菌
备注: --
产品目录号: #63914
稳定性: 瞬表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pYC25载体图谱



pYC25多克隆位点

载体简介

载体序列

LOCUS       Exported                6418 bp ds-DNA     circular SYN 14-AUG-2016
DEFINITION  synthetic circular DNA
KEYWORDS    pYC25
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6418)
  AUTHORS   Biofeng Lab.
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6418
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        666..737
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             738..1598
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1753..2372
                     /direction=RIGHT
                     /note="pBR322"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2681..2711
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2719..2735
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2743..2759
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2780..2798
                     /note="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2816..3214
                     /gene="S. cerevisiae TEF1"
                     /note="TEF1 promoter"
                     /note="promoter for EF-1-alpha"
     misc_feature    3218..3235
                     /note="MCS"
     primer_bind     3218..3234
                     /note="SK primer"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3238..3954
                     /codon_start=1
                     /note="YFP"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(3993..4009)
                     /note="KS primer"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(4424..4442)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4452..4468)
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      4613..5068
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     CDS             complement(5199..6002)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /note="URA3"
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(6003..6218)
                     /gene="S. cerevisiae URA3"
                     /note="URA3 promoter"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagcaggt tagactaaca tgcaaaaagt ttatattcat ttttgtagtt attcttgttt
      121 tcattaggat gattacacac tgtggatgtg gattaatcga tcattgttaa tttagtgtag
      181 ctactaccat ttcaaacaaa acgtaaattc ttgcagtcgt actggatctg tgaatctatt
      241 agtatatatg aattaaagta gcttgtacat attattctgt tgaatcatat cgcagagcat
      301 tggttgaaat ccaaaatata aaaatgtaat atcacaaaaa ataatactaa ttctaacatt
      361 aatgggtcag atttttagtg aatacttaaa tttataatat cgtctattta agctagcaaa
      421 tggaacaaca tttaaagtaa gaacatcata tctacatgaa aatgtatatt tcaatctgac
      481 taataacgca gagcacatct ttcagtgatg tctgtcacat gatcaaaaag aattgtattt
      541 aaataattca taataaaagc ttaaaaaatt acaataatga aaataaagta aataatgaca
      601 tgggtaagag ttccgaaata gaatcttagt gtacaaaaca aaaattgcat cattagagat
      661 ccccacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
      721 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
      781 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
      841 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
      901 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
      961 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     1021 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     1081 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     1141 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     1201 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     1261 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     1321 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     1381 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     1441 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     1501 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     1561 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     1621 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     1681 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     1741 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     1801 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     1861 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     1921 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     1981 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     2041 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     2101 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     2161 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     2221 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     2281 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     2341 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     2401 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     2461 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     2521 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     2581 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     2641 caattaatgt gagttacctc actcattagg caccccaggc tttacacttt atgcttccgg
     2701 ctcctatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     2761 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctcatag
     2821 cttcaaaatg tttctactcc ttttttactc ttccagattt tctcggactc cgcgcatcgc
     2881 cgtaccactt caaaacaccc aagcacagca tactaaattt cccctctttc ttcctctagg
     2941 gtgtcgttaa ttacccgtac taaaggtttg gaaaagaaaa aagagaccgc ctcgtttctt
     3001 tttcttcgtc gaaaaaggca ataaaaattt ttatcacgtt tctttttctt gaaaattttt
     3061 tttttgattt ttttctcttt cgatgacctc ccattgatat ttaagttaat aaacggtctt
     3121 caatttctca agtttcagtt tcatttttct tgttctatta caactttttt tacttcttgc
     3181 tcattagaaa gaaagcatag caatctaatc taagttttct agaactagtg gatcctaatg
     3241 agtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat
     3301 gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa
     3361 cttaccctta aattgatttg cactactgga aaactacctg ttccatggcc aacacttgtc
     3421 actactttgg gttatggtct aatgtgcttt gctagatacc cagatcatat gaaacggcat
     3481 gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa
     3541 gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat
     3601 agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg
     3661 gaatacaact ataactcaca caatgtatac atcactgcag acaaacaaaa gaatggaatc
     3721 aaagctaact tcaaaattag acacaacatt gaagatggag gtgttcaact agcagaccat
     3781 tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg
     3841 tcctatcaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt
     3901 gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa atagagatcc
     3961 cccgggctgc aggaattcga tatcaagctt atcgataccg tcgacctcga gaacacaacc
     4021 cacagctacc accatcaaca atatttatat atataacgta cacatagaaa tcacacaaac
     4081 agagtattta ttcttaacta catgaactac catcagaccg tctgggccac tatataatgt
     4141 gccattcata aacgtgatca ctttacgtag caggcaaccc caggtgaaaa tttttcagcg
     4201 agctgccaga ttgtcaggtg aaaaactgaa aaaaacttct gggcgatgag cttgtggcgg
     4261 gaaattaagt atataaagca gttagtttct tctgcttctt gtggttctgg gtattcttgt
     4321 aatatttgga agaatagaaa atcaagacta ctgcctttct tttcatatta tagaggaaga
     4381 aatacgcacg aacacgatat agaggtaaag gtacccaatt cgccctatag tgagtcgtat
     4441 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     4501 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     4561 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg cgacgcgccc
     4621 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     4681 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     4741 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     4801 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     4861 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     4921 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     4981 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     5041 tttaacaaaa tattaacgtt tacaatttcc tgatgcggta ttttctcctt acgcatctgt
     5101 gcggtatttc acaccgcata gggtaataac tgatataatt aaattgaagc tctaatttgt
     5161 gagtttagta tacatgcatt tacttataat acagtttttt agttttgctg gccgcatctt
     5221 ctcaaatatg cttcccagcc tgcttttctg taacgttcac cctctacctt agcatccctt
     5281 ccctttgcaa atagtcctct tccaacaata ataatgtcag atcctgtaga gaccacatca
     5341 tccacggttc tatactgttg acccaatgcg tctcccttgt catctaaacc cacaccgggt
     5401 gtcataatca accaatcgta accttcatct cttccaccca tgtctctttg agcaataaag
     5461 ccgataacaa aatctttgtc gctcttcgca atgtcaacag tacccttagt atattctcca
     5521 gtagataggg agcccttgca tgacaattct gctaacatca aaaggcctct aggttccttt
     5581 gttacttctt ctgccgcctg cttcaaaccg ctaacaatac ctgggcccac cacaccgtgt
     5641 gcattcgtaa tgtctgccca ttctgctatt ctgtatacac ccgcagagta ctgcaatttg
     5701 actgtattac caatgtcagc aaattttctg tcttcgaaga gtaaaaaatt gtacttggcg
     5761 gataatgcct ttagcggctt aactgtgccc tccatggaaa aatcagtcaa gatatccaca
     5821 tgtgttttta gtaaacaaat tttgggacct aatgcttcaa ctaactccag taattccttg
     5881 gtggtacgaa catccaatga agcacacaag tttgtttgct tttcgtgcat gatattaaat
     5941 agcttggcag caacaggact aggatgagta gcagcacgtt ccttatatgt agctttcgac
     6001 atgatttatc ttcgtttcct gcaggttttt gttctgtgca gttgggttaa gaatactggg
     6061 caatttcatg tttcttcaac actacatatg cgtatatata ccaatctaag tctgtgctcc
     6121 ttccttcgtt cttccttctg ttcggagatt accgaatcaa aaaaatttca aagaaaccga
     6181 aatcaaaaaa aagaataaaa aaaaaatgat gaattgaatt gaaaagctgt ggtatggtgc
     6241 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     6301 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     6361 accgtctccg ggaggtgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcga
//

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