pBABE-HAII

价格:1250元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Addgene
载体名称: pBABE-HAII
质粒类型: 逆病毒载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: MSCV
载体大小: 5206 bp
5' 测序引物及序列: pBABE 5' primer 5'CTTTATCCAGCCCTCAC3'
3' 测序引物及序列: pBABE 3' primer 5'ACCCTAACTGACACACATTCC3'
载体标签:
载体抗性: 氨苄青霉素
筛选标记: Puromycin
克隆菌株: DH5α、HB101等
宿主细胞(系): 常规哺乳动物细胞系
备注:

推荐使用逆病毒包装载体:pUMVCpCMV-VSV-G
 
产品目录号: #14738
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 逆转录病毒

载体质粒图谱和多克隆位点信息

pBABE-HAII载体图谱



pBABE-HAII多克隆位点

载体简介

载体序列

LOCUS       pBABE HAII	5206 bp 	DNA	SYN
DEFINITION  pBABE HAII
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..5206
                     /organism="pBABE HAII"
                     /mol_type="other DNA"
     misc_feature    12..479
                     /label="5_LTR2"
     misc_feature    18..479
                     /label="3MoMuLV_LTR"
     misc_feature    549..1350
                     /label="psi_plus_pack"
     misc_feature    1318..1334
                     /label="pBABE_5_primer"
     misc_feature    complement(1445..1465)
                     /label="pBABE_3_primer"
     misc_feature    complement(1451..1666)
                     /label="SV40_enhancer"
     promoter        1463..1731
                     /label="SV40_promoter"
     rep_origin      1630..1707
                     /label="SV40_origin"
     misc_feature    1692..1711
                     /label="SV40pro_F_primer"
     CDS             complement(1765..2439)
                     /label="ORF frame 2"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGRAVLRALDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLFAKA*"
     gene            1789..2388
                     /label="puro (variant)"
                     /gene="puro (variant)"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGRAVLRALDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLFAKA*"
     CDS             1789..2388
                     /label="ORF frame 1"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA*"
     misc_feature    2487..3080
                     /label="3MoMuLV_LTR"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA*"
     misc_feature    2529..3080
                     /label="5_LTR2"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA*"
     gene            complement(4218..5078)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA*"
     CDS             complement(4218..5078)
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(5120..5148)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
   61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
  121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
  181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
  241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
  301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
  361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
  421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
  481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
  541 taagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
  601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
  661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
  721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
  781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
  841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt
  901 ctgactgtgt ttctgtattt gtctgaaaat tagggccaga ctgttaccac tcccttaagt
  961 ttgaccttag atcactggaa agatgtcgag cggctcgctc acaaccagtc ggtagatgtc
 1021 aagaagagac gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg
 1081 ccgcgagacg gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca
 1141 cctggcccgc atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct
 1201 tttgaccccc ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttctt
 1261 ccatccgcgc cgtctctccc ccttgaacct cctctttcga ccccgcctca atcctccctt
 1321 tatccagccc tcactccttc tctaggcgcc ggccggatca atggcttcaa gttatccata
 1381 tgatgtgccc gactatgcct cacttggagg accctctgga tccggaattc tccgtcgacc
 1441 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
 1501 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
 1561 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
 1621 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
 1681 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
 1741 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttaccat gaccgagtac
 1801 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
 1861 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
 1921 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
 1981 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
 2041 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
 2101 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
 2161 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
 2221 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
 2281 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgagtgc
 2341 ccgaaggacc gcgcgacctg gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac
 2401 gacccgcagc gcccgaccga aaggagcgca cgaccccatg catcgataaa ataaaagatt
 2461 ttatttagtc tccagaaaaa ggggggaatg aaagacccca cctgtaggtt tggcaagcta
 2521 gcttaagtaa cgccattttg caaggcatgg aaaaatacat aactgagaat agagaagttc
 2581 agatcaaggt caggaacaga tggaacagct gaatatgggc caaacaggat atctgtggta
 2641 agcagttcct gccccggctc agggccaaga acagatggaa cagctgaata tgggccaaac
 2701 aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag
 2761 atgcggtcca gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag
 2821 gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt
 2881 tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac tcggggcgcc
 2941 agtcctccga ttgactgagt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt
 3001 gcatccgact tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc
 3061 gtcagcgggg gtctttcatt tccgacttgt ggtctcgctg ccttgggagg gtctcctctg
 3121 agtgattgac tacccgtcag cgggggtctt cacatgcagc atgtatcaaa attaatttgg
 3181 ttttttttct taagtattta cattaaatgg ccatagttgc attaatgaat cggccaacgc
 3241 gcggggagag gcggtttgcg tattggcgct cttccgcttc ctcgctcact gactcgctgc
 3301 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
 3361 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
 3421 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
 3481 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
 3541 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
 3601 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
 3661 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
 3721 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
 3781 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
 3841 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
 3901 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
 3961 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
 4021 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
 4081 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
 4141 agatcctttt aaattaaaaa tgaagtttgc ggccgcaaat caatctaaag tatatatgag
 4201 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
 4261 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
 4321 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
 4381 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
 4441 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
 4501 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
 4561 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
 4621 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
 4681 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
 4741 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
 4801 atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc
 4861 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
 4921 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
 4981 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
 5041 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
 5101 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
 5161 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccac
//

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