| 出品公司: | Addgene |
|---|---|
| 载体名称: | pBABE-HAII |
| 质粒类型: | 逆病毒载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | MSCV |
| 载体大小: | 5206 bp |
| 5' 测序引物及序列: | pBABE 5' primer 5'CTTTATCCAGCCCTCAC3' |
| 3' 测序引物及序列: | pBABE 3' primer 5'ACCCTAACTGACACACATTCC3' |
| 载体标签: | 无 |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | Puromycin |
| 克隆菌株: | DH5α、HB101等 |
| 宿主细胞（系）: | 常规哺乳动物细胞系 |
| 备注: |
推荐使用逆病毒包装载体：pUMVC、pCMV-VSV-G 。 |
| 产品目录号: | #14738 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 逆转录病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pBABE HAII 5206 bp DNA SYN
DEFINITION pBABE HAII
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..5206
/organism="pBABE HAII"
/mol_type="other DNA"
misc_feature 12..479
/label="5_LTR2"
misc_feature 18..479
/label="3MoMuLV_LTR"
misc_feature 549..1350
/label="psi_plus_pack"
misc_feature 1318..1334
/label="pBABE_5_primer"
misc_feature complement(1445..1465)
/label="pBABE_3_primer"
misc_feature complement(1451..1666)
/label="SV40_enhancer"
promoter 1463..1731
/label="SV40_promoter"
rep_origin 1630..1707
/label="SV40_origin"
misc_feature 1692..1711
/label="SV40pro_F_primer"
CDS complement(1765..2439)
/label="ORF frame 2"
/translation="MGSCAPFGRALRVVGRASGTGLAGHAPGRAVLRALDVGGDGEAE
PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
KLFAKA*"
gene 1789..2388
/label="puro (variant)"
/gene="puro (variant)"
/translation="MGSCAPFGRALRVVGRASGTGLAGHAPGRAVLRALDVGGDGEAE
PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
KLFAKA*"
CDS 1789..2388
/label="ORF frame 1"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
ETSAPRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA*"
misc_feature 2487..3080
/label="3MoMuLV_LTR"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
ETSAPRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA*"
misc_feature 2529..3080
/label="5_LTR2"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
ETSAPRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA*"
gene complement(4218..5078)
/label="Ampicillin"
/gene="Ampicillin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
ETSAPRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA*"
CDS complement(4218..5078)
/label="ORF frame 3"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter complement(5120..5148)
/label="AmpR_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
541 taagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt
901 ctgactgtgt ttctgtattt gtctgaaaat tagggccaga ctgttaccac tcccttaagt
961 ttgaccttag atcactggaa agatgtcgag cggctcgctc acaaccagtc ggtagatgtc
1021 aagaagagac gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg
1081 ccgcgagacg gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca
1141 cctggcccgc atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct
1201 tttgaccccc ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttctt
1261 ccatccgcgc cgtctctccc ccttgaacct cctctttcga ccccgcctca atcctccctt
1321 tatccagccc tcactccttc tctaggcgcc ggccggatca atggcttcaa gttatccata
1381 tgatgtgccc gactatgcct cacttggagg accctctgga tccggaattc tccgtcgacc
1441 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
1501 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
1561 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
1621 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
1681 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
1741 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttaccat gaccgagtac
1801 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
1861 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
1921 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
1981 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
2041 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
2101 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
2161 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
2221 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
2281 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgagtgc
2341 ccgaaggacc gcgcgacctg gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac
2401 gacccgcagc gcccgaccga aaggagcgca cgaccccatg catcgataaa ataaaagatt
2461 ttatttagtc tccagaaaaa ggggggaatg aaagacccca cctgtaggtt tggcaagcta
2521 gcttaagtaa cgccattttg caaggcatgg aaaaatacat aactgagaat agagaagttc
2581 agatcaaggt caggaacaga tggaacagct gaatatgggc caaacaggat atctgtggta
2641 agcagttcct gccccggctc agggccaaga acagatggaa cagctgaata tgggccaaac
2701 aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag
2761 atgcggtcca gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag
2821 gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt
2881 tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac tcggggcgcc
2941 agtcctccga ttgactgagt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt
3001 gcatccgact tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc
3061 gtcagcgggg gtctttcatt tccgacttgt ggtctcgctg ccttgggagg gtctcctctg
3121 agtgattgac tacccgtcag cgggggtctt cacatgcagc atgtatcaaa attaatttgg
3181 ttttttttct taagtattta cattaaatgg ccatagttgc attaatgaat cggccaacgc
3241 gcggggagag gcggtttgcg tattggcgct cttccgcttc ctcgctcact gactcgctgc
3301 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
3361 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
3421 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
3481 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
3541 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
3601 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
3661 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
3721 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
3781 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
3841 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
3901 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
3961 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
4021 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
4081 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
4141 agatcctttt aaattaaaaa tgaagtttgc ggccgcaaat caatctaaag tatatatgag
4201 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
4261 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
4321 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
4381 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
4441 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
4501 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
4561 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
4621 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
4681 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
4741 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
4801 atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc
4861 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
4921 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
4981 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
5041 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
5101 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
5161 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccac
//
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